• 제목/요약/키워드: RAPD Markers

검색결과 303건 처리시간 0.036초

RAPD 방법을 이용한 국내 수집 인삼 (Panax ginseng C. A. Meyer)의 다양성 분석 (Analysis of Diversity of Panax ginseng Collected in Korea by RAPD Technique)

  • 서상덕;육진아;차선경;김현호;성봉재;김선익;최재을
    • 한국약용작물학회지
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    • 제11권5호
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    • pp.377-384
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    • 2003
  • 본 연구는 고려인삼 재래종의 유전적 차이를 RAPD marker로 평가하였다. 재래종은 우리나라 주요 인삼재배 단지인 괴산, 금산, 남원, 포천, 양주, 연천, 영주로부터 수집한 인삼에서 10개체를 임의 선발하여 사용하였다. 9개 지역의 90개체를 대상으로 RAPD분석을 한 결과 48개의 primer 중 OPA 7, OPA 13, URP 2, URP 3, UBC 3의 5개 primer가 재현성이 있고 재래종 내 개체간에도 다형성인 band를 보였다. 재래종 집단간보다 재래종 내에서 유전적 다양성이 낮았다. 재래종 집단 간 또는 재래종 집단내의 유전적 차이가 있다는 것은 이 집단들이 헤테로라는 것을 의미한다. 이러한 결과는 우리나라에서 재배중인 고려 인삼은 유전 자원의 혼합으로 헤테로라는 것을 암시한다. 선발된 5개의 primer를 이용하여 90개체를 집괴분석한 결과 국내 재래종 인삼은 5개군으로 그리고 3개의 아군으로 구분되었다. 국내 재래종 인삼은 유전적 변이가 크므로 인삼 육종을 위한 재료로 이용될 수 있을 것이다.

PCR-RAPD 기법을 이용한 세포 유형이 다른 무릇 (Scilla scilloides Complex) 체세포클론의 유전적 분석 (Genetic Analysis of Somaclones Derived from Different Cytotype Plants of Scilla scilloides Complex using RAPD)

  • 오정순;방재욱
    • 식물조직배양학회지
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    • 제26권4호
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    • pp.235-240
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    • 1999
  • 무릇 (S. scilloides Complex)에서 게놈 유형이 다른 AA, BB 및 AABB 게놈 식물의 조직배양을 통하여 유도된 재분화체를 대상으로 RAPD기법을 이용한 유전적 분석을 통해 게놈의 안정성과 게놈 유형에 따른 차이를 분석한 결과, 적용한 20가지의 primer중 Al, A2및 A3에서 게놈 유형에 따른 다형현상을 관찰할 수 있었다. RAPD분석 결과 AA게놈에서 39.2%로 다형성이 세 가지 유형 중 가장 낮게 나타났으며, BB 게놈에서는 72.3%로 가장 높은 다형성을 보였고, AABB 게놈에서는 45.7%의 다형성이 관찰되었다. AA유형에서 관찰된 총 110개의 밴드 중 특이 밴드가 A3에서 하나 나타나 0.9%의 변이율을 보였으며, BB 게놈의 경우 총 116개의 밴드 중 특이 밴드는 A3에서 5개 나타나 4.3%의 변이율을 보여 주었고, AABB유형에서는 총 103개의 밴드 중 특이 밴드가 Al에서 2개, A2에서 1개, A3에서 2개로 총 5개가 나타나 4.9%의 변이율을 나타내었다. RAPD 분석은 게놈 유형이 다른 무릇 체세포클론의 유전적인 안정성 분석에 유용하게 이용될 수 있다.

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Genetic Analysis of Haimen Chicken Populations Using Decamer Random Markers

  • Olowofeso, O.;Wang, J.Y.;Zhang, P.;Dai, G.J.;Sheng, H.W.;Wu, R.;Wu, X.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권11호
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    • pp.1519-1523
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    • 2006
  • Through a screening and selection approach method, decamer random markers were used in a technique called random amplified polymorphic DNA (RAPD) assay with 252 genomic DNAs isolated from four major Haimen chicken populations: Rugao (62), Jiangchun (62), Wan-Nan (63) and Cshiqishi (65). A total of 3-score decamer random primers (S241-S260, S1081-S1100 and S1341-S1360) were employed in the preliminary RAPD-polymerase chain reaction (RAPD-PCR) assay with 50 random template DNA samples from all the populations. Four (6.67%) of the primers that produced obvious polymorphic patterns, interpretable and reproducible bands were selected and used with both the individual DNAs from each population and with pooled DNA samples of the four populations in subsequent analyses. The selected primers produced a total of 131 fragments with molecular size ranging from 835 to 4,972 base pairs (bp) when used with the individual DNAs; 105 (80.15%) of these fragments were polymorphic. With the pooled DNAs, 47 stable and characteristic bands with molecular size ranging from 840 to 4,983 bp, of which 23 (48.94%) polymorphic, were also generated. The band-sharing coefficient (BSC) calculated for the individuals in the population and among populations of bulked samples was between 0.8247 (Rugao) and 0.9500 (Cshiqishi); for pairwise populations, it was between 0.7273 (Rugao vs. Wan-Nan) and 0.9367 (Jiangchun vs. Cshiqishi) chicken populations. Using the BSC for individual and pairwise populations, the Nei's standard genetic distances between the chicken populations were determined and ranged from 0.0043 (Jiangchun vs. Cshiqishi) to 0.1375 (Rugao vs. Cshiqishi). The reconstructed dendrogram linked the Jiangchun and Cshiqishi chickens as closely related populations, followed by Wan-Nan, while the Rugao was the most genetically distant among the populations.

Biochemical Characterization and Genetic Diversity of Pongamia pinnata (L.) Pierre in Eastern India

  • Kumari, Kanchan;Sinha, Amrita;Singh, Sanjay;Divakara, B.N.
    • Journal of Forest and Environmental Science
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    • 제29권3호
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    • pp.200-210
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    • 2013
  • Biochemical characteristics of 24 Pongamia pinnata genotypes (candidate plus trees) from three agroclimatic zones were estimated and molecular characterization through RAPD markers was done. Various biochemical characters viz. seed oil, total carbohydrates, protein, acid value and Iodine number recorded significant variation among different genotypes. The highest seed oil content was 41.87% while seeds of 14 genotypes recorded above average (32.11%) for the trait. Seed oil and protein content exhibited a significant positive correlation and moderate heritability. Out of the initially selected twenty-five random primers, twenty-two RAPD primers were found to be highly reproducible and produced a total of 183 loci of which 147 (80.32%) loci were polymorphic. Percentage of polymorphism varied from 44% to 100% with an average of 80.62%. High level of genetic variation was found among different genotypes of P. pinnata. Both molecular and oil content (biochemical) markers appeared useful in analyzing the extent of genetic diversity in Pongamia and the result of these analyses will help to better understand the genetic diversity and relationship among populations. Overall, the Pongamia genotypes included in the study showed a correlation with their geographical origins such that genotypes from the same region tend to have higher genetic similarity as compared to those from different regions. However, in UPGMA based Nei's analysis, some genotypes were found not to be grouped based on geographical origins possibly due to the exchange of germplasm over time between farmers across the regions. The results from oil content analyses showed that several genotypes in 'Central and Western Plateau' agroclimatic zone of Jharkhand displayed a good potential for high oil content. The study provides insight about P. pinnata populations in Jharkhand (India) and constitutes a set of useful background information that can be used as a basis for future breeding strategy and improvement of the species.