• Title/Summary/Keyword: RAP

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Identification of Sex-specific Expression Markers in the Giant Tiger Shrimp (Penaeus monodon)

  • Khamnamtong, Bavornlak;Thumrungtanakit, Supaporn;Klinbunga, Sirawut;Aoki, Takashi;Hirono, Ikuo;Menasveta, Piamsak
    • BMB Reports
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    • v.39 no.1
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    • pp.37-45
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    • 2006
  • Bulked segregant analysis (BSA) and AFLP were used for isolation of genomic sex determination markers in Penaeus monodon. A total of 256 primer combinations were tested against 6-10 bulked genomic DNA of P. monodon. Five and one candidate female- and male-specific AFLP fragments were identified. Female-specific fragments were cloned and further characterized. SCAR markers derived from FE10M9520, FE10M10725.1, FE10M10725.2 and FE14M16340 provided the positive amplification product in both male and female P. monodon. Further analysis of these markers using SSCP and genome walk analysis indicated that they were not sex-linked. In addition, sex-specific (or differential) expression markers in ovaries and testes of P. monodon were analyzed by RAP-PCR (150 primer combinations). Twenty-one and fourteen RAP-PCR fragments specifically/differentially expressed in ovaries and testes of P. monodon were successfully cloned and sequenced. Expression patterns of 25 transcripts were tested against the first stranded cDNA of ovaries and testes of 3-month-old and broodstock-sized P. monodon (N = 5 and N = 7 - 10 for females and N = 4 and N = 5 - 7 for males, respectively). Five (FI-4, FI-44, FIII-4, FIII-39 and FIII-58) and two (M457-A01 and MII-51) derived RAP-PCR markers revealed female- and male-specific expression patterns in P. monodon. Surprisingly, MII-5 originally found in testes showed a higher expression level in ovaries than did testes of juvenile shrimps but a temporal female-specific pattern in P. monodon adults.

Changes of Triglyceride Composition in Adlay Powder during Storage (율무가루 저장 중 Triglyceride 조성의 변화)

  • Han, Ji-Sook;Rhee, Sook-Hee;Cheigh, Hong-Sik
    • Korean Journal of Food Science and Technology
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    • v.23 no.1
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    • pp.102-108
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    • 1991
  • Raw adlay powder(RAP)was prepared and the changes of triglyceride composition in RAP lipid during storage at $35^{\circ}C$ for six months were studied. The RAP lipid consisted of 28 kinds of triglycerides and the major triglyceride in RAP lipid were those of OOL(24.14%), OLL(24.06%), OOO(12.58%), POL(9.01%), POO(8.87%), LLL(7.91%)and PLL(5.80%). During the storage at $35^{\circ}C$ for six months, the relative amounts of the triglyceride containing linoleic acid(OLL : 15.25%, LLL : 2.93%)considerably decreased, but those containing oleic acid(OOO : 23.77%. POO : 12.62%) increased. The triglycerides of LLA(0.44%)and PPLn(0.12%)disappeared during the storage.

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The Behavior of Rammed Aggregate Piers (RAP) in Soft Ground (I) (연악지반의 쇄석다짐말뚝에 대한 거동 분석 (I))

  • Bae, Kyung-Tae;Lee, Chong-Kyu
    • Journal of the Korean Geotechnical Society
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    • v.23 no.4
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    • pp.169-183
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    • 2007
  • Numerical analysis was performed to investigate the behavior of rammed aggregate piers (RAP) in soft ground with various interface conditions, area replacement ratio, aspect ratio and surcharge loads of pile and soil. And field modulus load test was carried out to predict the input parameters. Field prototype (unit cell) tests are in progress to compare the result of numerical analysis. Also a modified load transfer equation of RAP on soft foundation was proposed. According to the results, the behavior of RAP depended on such as interface conditions, settlement characteristics (free strain) and stress concentration ratio. On the other hand, maximun stress concentration ratio increased as area replacement ratio and aspect ratio increased, and it was remarkably affected by surcharge loads.

A PSYCHIATRIC STUDY ON THE CHILDREN WITH RECURRENT ABDOMINAL PAIN (반복적 복통 환아의 정신과적 연구(反復的 腹痛 患兒의 精神科的 硏究))

  • Choi, Jin-Sook;Hong, Kang-E;Seo, Jeong-Kee
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.1 no.1
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    • pp.117-129
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    • 1990
  • 35 children(17 boys, 18 girls) with Recurrent Abdominal pain(RAP) without apparent organic causes, referrd from pediatric department of Seoul National University Children's Hospital were studied during 1988. 7. 1 ${\sim}$ 1989. 2. 28. The aims of this study are to examine clinical characteristics of the patients with RAP, to find possible factors influencing the onset and the course of the disorder, to evaluate the psychological status and the relationship with psychiatric diagnoses. The study subjects were compared with the age and sex matched control subjects on measures of social and school functioning, family-environment, behavior traits by CBCL and parent-environment rating scale. All patients and their mothers were interviewed by a child psychiatrist. The results were as follows ; 1) Abdominal pain was found to be more frequent when with a care taker, and related with traumatic life stresses such as separation from parents, death of family members. 2) Family members of the patients with RAP also had a high rate of a history of gastrointestinal dysfunctions with could not be attributed to organic etiologies. 3) Among 35 patients with RAP, 16 were diagnosed as psychiatric disorders. They were adjustment disorders (4), overanxious disorders (4), depression (2), tic disorders (2), attention deficit disorders (2), separation anxiety disorders (1), enuresis (1). 4) On CBCL, the patients with RAP were more internalized, socially less competent and less active compared with the control subjects. 5) According to the parent-environment rating scale, the patients with RAP had more conflicts with their parents about control issues. Family members of the patients with RAP were socially less competent and less abjustable. The parents of the patients with RAP were more affectionate and spent more time with their children but they were unfair and vague on instruction and discipline.

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Analysis of Changes in Hemodynamic Values after Modified Fontan Procedure (변형 Fontan 술후 혈류역학치 변화추이 분석)

  • 안재호
    • Journal of Chest Surgery
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    • v.21 no.5
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    • pp.816-827
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    • 1988
  • Since 1978, We have experienced 87 cases of Fontan operations and the candidates of that increased in numbers recently with the improvement of the diagnostic and operative technique. We studied the prerequisite factors and hemodynamics of 22 cases of Fontan operations, done during the last one year period, which were 3 tricuspid atresia, 16 functional single ventricle and 3 anatomic single ventricle. The mean age was 68 months and the mortality rate 24%, and 9 patients of under 4 years of age were operated with 22.2% mortality rate, but the youngest, 16 months of age, patient survived well without problems. The preoperative pulmonary artery pressure[PAP], pulmonary vascular resistance[PVR] and postoperative right atrial pressure[RAP], left atrial pressure[LAP] value influenced the mortality, but age, preoperative Hb, preoperative PaO2 and pulmonary artery index[PAI] did not. There were favorable survival tendency in under 15mmHg of preop. PAP, 2a of preop. PVR and under 25cmHyO of postop. RAP, under 15cmHyO of postop LAP. The younger, the more pleural effusion and the longer postoperative admission days. The higher preop. Hb related to the higher postop. transpulmonary pressure gradient and the lower preop. PaO2 and PAI. The higher preop. PaO2, the less pleural effusion and postop. admission days. Preop. PAP closely related to preop. PVR and postop. LAP and high PVR increased the pleural effusion and postop. admission days. The larger PAI, the larger CI. We concluded that there were so many factors influencing the postoperative condition, but preop. PAP, PVR, Hb, postop. RAP and LAP were the most ones.

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The Preload-Responsive Regulation of Cardiac Output in Total Artificial Heart Using Dual Adaptive Controller (2중 적응제어방식에 의한 전치환 인공심장의 전부하에 민감한 심박출량 조절)

  • Lee, Sang-Hoon;Kim, In-Young;Ahn, Hyuk;Min, Byoung-Goo
    • Journal of Biomedical Engineering Research
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    • v.14 no.1
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    • pp.41-50
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    • 1993
  • This paper proposes an adaptive technique for the cardiac output regulation of a pendulum type electromechanical TAH. This techinque, which consists of two RLSE's and two PASTC' 5, performs Its controllability over the TAIB so that the m(RAP) and m(LAP) values re- main close to their desired values under she assumption that the variation of m(RAP) and m (LAP) are dominated by the variation of C.0. and the difference between the left and right ventricular output, respectively. To evaluate the performance of the proposed control system, a simulation is performed by using a human model which contains physiologic, drug and treatment, artificial heart and noise models. As a result, dual adaptive controller showed that abnormal m(LAP) and m(RAP) could be recovered to the normal range within 10minute and maintained desired value in steady state. The operation of this controller prored to be robust in spite of the rapid variation of human status.

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Propellant Characteristics used for a Rocket-Assisted Projectile with Aluminium Contents (알루미늄 함량에 따른 로켓보조추진탄용 추진제 특성)

  • Jeong, Jae-Yun;Choi, Sung-Han
    • Journal of the Korean Society of Propulsion Engineers
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    • v.23 no.5
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    • pp.60-66
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    • 2019
  • In this report, the process characteristic(viscosity), mechanical properties, combustion characteristics, ground and flight test results of propellants used for a rocket-assisted projectile are described according to several aluminum contents. As the aluminum content increased, initial viscosity decreased, viscosity build-up accelerated, and combustion rate and pressure exponent decreased. In the ground fire test, the total impulse of the rocket-assisted projectiles containing 10 wt% of aluminum were 5% higher than that of the rocket-assisted projectiles containing 2 wt% and 18 wt% of aluminum. The motor efficiency compared to the theoretical performance was 85.6% with 18 wt% of aluminum, the lowest value among the propellant compositions.

Correlation of Binder GPC Characteristics and Mechanical Properties of Hot-Mix Recycled Asphalt Mixtures (재생혼합물의 바인더 GPC특성과 역학적 특성과의 상관성 연구)

  • Kim, Kwang-Woo;Hong, Sang-Ki;Cho, Mun-Jin;Doh, Young-Soo
    • International Journal of Highway Engineering
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    • v.7 no.1 s.23
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    • pp.11-20
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    • 2005
  • This study examined the correlation between mechanical properties and LMS(Large molecular size) of binders in hot-recycled asphalt mixtures. Hot-recycled asphalt mixtures were manufactured by various mixing methods. Laboratory tests including indirect tensile strength, wheel tracking test and Kim test were performed for each recycled mixture. Gel-permeation chromatography (GPC) analysis was performed for the binders coated on virgin and old (RAP) aggregates separately. For the purpose of binder aging analysis, a round-shaped virgin coarse aggregates (13mm gravel) were introduced in recycled mixtures. This makes possible to distinguish the virgin aggregates from RAP aggregates in recycled mixtures for GPC sampling. Results of GPC showed that there was significant difference in aging level between the binder coated on RAP and that of virgin aggregates in the same recycled mixture. Regression analysis was performed to correlate mechanical properties to LMS ratio. Results showed that most of the mechanical properties had relatively good correlation with LMS. This trend agree with LMS increase up to some degree, but fails for further LMS increase.

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Rapping as a Means of Improving Self-Expression: A Case Study of Adolescent Survivors of Childhood Cancer (소아암 완치 청소년의 자기표현 경험을 위한 랩 만들기 사례)

  • Choi, Jieun
    • Journal of Music and Human Behavior
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    • v.16 no.2
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    • pp.27-51
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    • 2019
  • This case study aimed to investigate changes in self-expression following participation in a rap making program with adolescent survivors of childhood cancer. The rap making program was constructed based on the contextual support music therapy model. Three adolescent survivors of childhood cancer participated in six 80-minute individual sessions. During the sessions, each participant engaged in the following tasks: song discussion, lyric creation, and rapping over a selected beat. At pre and posttest, the Self-Expression Scale was completed by participants. Their verbal expressions lyrics were observed during the sessions, and individual interviews with the participants were conducted at the completion of the program. The results demonstrated that the mean rating of the Self-Expression Scale increased after the rap making intervention. Analysis of the participants' verbal expressions and lyrics demonstrated that participants were experiencing difficulties adjusting to school that they wanted to resolve. Furthermore, the analysis of the interviews at posttest found that participants experienced positive changes in self-perception, self-expression, and expectations for their future, compared to the pretest when the participants expressed negative self-perceptions due to difficulties in interpersonal relationships at school and physical limitations. This indicates that rap making can be an effective resource for providing this population with the means to recognize positive attributes about themselves and improve self-expression.

Characteristics of Fatigue Resistance of Recycled Asphalt Concretes by Modified Mixing Process (재생 아스팔트 콘크리트 혼합물의 혼합방법 개선에 따른 피로저항 특성)

  • Doh, Young-Soo;Ko, Tae-Young;Kim, Kwang-Woo
    • International Journal of Highway Engineering
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    • v.8 no.4 s.30
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    • pp.135-143
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    • 2006
  • This study evaluated the fatigue resistance characteristics of hot-mix recycled asphalt mixtures which were prepared by a new blending method. Since the binder of RAP shows higher viscosity without being rejuvenated in the ordinary recycled mixture, this new(modified) blending method was developed for obtaining more uniform level of binder viscosity in the recycled mixture. Cold-planned RAP was collected and mix design was performed using 15% RAP content for two virgin aggregate, gneiss and granite. Penetration grade of 60-80 asphalt was used in mixing recycled mixture together with a polymer modifier, LDPE. Indirect tensile fatigue tests were carried out to evaluate characteristics of fatigue resistance of performance of recycled asphalt mixtures. The recycled mixtures with LDPE 6% showed higher repeated loading fatigue life. Fatigue life ratio of new(N) mixing method mixtures was approximately 0.6-0.7 before aging and 0.8-1.0 after aging treatment of ordinary(O) mixing method mixture. This means the N mixture becomes stronger with aging process increase. If further aging is treated, N mixture may be showing stronger resistance than O mixture.

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