• Journal of the Korean Mathematical Society
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• v.53 no.2
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• pp.403-414
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• 2016

Let R be a ring, and let M be a left R-module. If M is Rad-supplementing, then every direct summand of M is Rad-supplementing, but not each factor module of M. Any finite direct sum of Rad-supplementing modules is Rad-supplementing. Every module with composition series is (Rad-)supplementing. M has a Rad-supplement in its injective envelope if and only if M has a Rad-supplement in every essential extension. R is left perfect if and only if R is semilocal, reduced and the free left R-module $(_RR)^{({\mathbb{N})}$ is Rad-supplementing if and only if R is reduced and the free left R-module $(_RR)^{({\mathbb{N})}$ is ample Rad-supplementing. M is ample Rad-supplementing if and only if every submodule of M is Rad-supplementing. Every left R-module is (ample) Rad-supplementing if and only if R/P(R) is left perfect, where P(R) is the sum of all left ideals I of R such that Rad I = I.

• Korean Journal of Microbiology
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• v.46 no.2
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• pp.122-126
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• 2010

RAD53 functions as an effector kinase of checkpoint pathways in Saccharomyces cerevisiae, which plays a central role to regulate many downstream cellular processes in response to DNA damage. It also involves in transcriptional activation of various genes including RNR genes which encode the key enzyme required for dNTP synthesis. In this study, we identified CYC8 as a suppressor for the hydroxyurea sensitivity of $rad53{\Delta}$ mutation. $Rad53{\Delta}$ mutant transformed with a multi-copy plasmid containing CYC8 showed increased hydroxyurea resistance. In contrast, TUP1 which forms a complex with CYC8 did not function as a suppressor. In the case of mutations, both $cyc8{\Delta}$ and $tup1{\Delta}$ suppressed hydroxyurea sensitivity of $rad53{\Delta}$. Since CYC8 can propagate as a prion in yeast, overexpression of CYC8 induced misfolding of the normal CYC8 proteins, resulting in dominant cyc8-phenotype. Therefore, it is suggested that CYC8 can act as a multi-copy suppressor due to its prion property. It was observed that the levels of RNR transcription were increased in the yeast strains containing either multi-copies of CYC8 gene or $cyc8{\Delta}$ mutation, suggesting that the increased level of RNR will elevate the intracellular pools of dNTPs, which, in turn, suppress the phenotype of $rad53{\Delta}$ mutation.

• The Korean Journal of Nuclear Medicine
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• v.34 no.2
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• pp.144-153
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• 2000

Purpose: The purpose of this study was to evaluate the relationship between radiation-induced activation of DNA repair genes and radiation induced apoptosis in A431 cell line. Materials and Methods: Five and 25 Gys of gamma radiation were given to A431 cells by a Cs-137 cell irradiator. Apoptosis was evaluated by flow cytometry using annexin V-fluorescein isothiocyanate and propidium iodide staining. The expression of DNA repair genes was evaluated by both Northern and Western blot analyses. Results: The number of apoptotic cells increased with the increased radiation dose. It increased most significantly at 12 hours after irradiation. Expression of p53, p21, and hRAD50 reached the highest level at 12 hours after 5 Gy irradiation. In response to 25 Gy irradiation, hRAD50 and p21 were expressed maximally at 12 hours, but p53 and GADD45 genes showed the highest expression level after 12 hours. Conclusion: Induction of apoptosis and DNA repair by ionizing radiation were closely correlated. The peak time of inducing apoptosis and DNA repair was 12 hours in this study model. hRAD50, a recently discovered DNA repair gene, was also associated with radiation-induced apoptosis.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.13
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• pp.5211-5217
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• 2015

Background: We earlier used PCR-dHPLC for mutation analysis of BRCA1 and BRCA2. In this article we report application of targeted resequencing of 30 genes involved in hereditary cancers. Materials and Methods: A total of 91 patient samples were analysed using a panel of 30 genes in the Illumina HiScan SQ system. CLCBio was used for mapping reads to the reference sequences as well as for quality-based variant detection. All the deleterious mutations were then reconfirmed using Sanger sequencing. Kaplan Meier analysis was conducted to assess the effect of deleterious mutations on disease free and overall survival. Results: Seventy four of the 91 samples had been run earlier using the PCR-dHPLC and no deleterious mutations had been detected while 17 samples were tested for the first time. A total of 24 deleterious mutations were detected, 11 in BRCA1, 4 in BRCA2, 5 in p53, one each in RAD50, RAD52, ATM and TP53BP1. Some 19 deleterious mutations were seen in patients who had been tested earlier with PCR-dHPLC [19/74] and 5/17 in the samples tested for the first time, Together with our earlier detected 21 deleterious mutations in BRCA1 and BRCA2, we now had 45 mutations in 44 patients. BRCA1c.68_69delAG;p.Glu23ValfsX16 mutation was the most common, seen in 10/44 patients. Kaplan Meier survival analysis did not show any difference in disease free and overall survival in the patients with and without deleterious mutations. Conclusions: The NGS platform is more sensitive and cost effective in detecting mutations in genes involved in hereditary breast and/or ovarian cancers.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10457-10462
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• 2015

Homologous recombination repair (HRR) plays an important role in protection against carcinogenic factors. Genes regulating the HRR mechanisms may impair their functions and consequently result in increased cancer susceptibility. RAD 51 and XRCC3 are key regulators of the HRR pathway and genetic variability in these may contribute to the appearance and progression of various cancers including head and neck cancer (HNC). The aim of the present study was to compare the distribution of genotypes of RAD51 (135G/C, 172 G/T) and XRCC3 (Thr241Met) polymorphisms between HNC patients and controls. Each polymorphism was genotyped using the polymerase chain reaction-restriction fragment length polymerase (PCR-RFLP) technique in 200 pathologically confirmed HNC patients along with 150 blood samples from normal, disease free healthy individuals. We observed that homozygous variant CC genotype of RAD51 135G/C was associated with a 2.5 fold increased HNC risk (OR=2.5; 95%CI=0.69-9.53; p<0.02), while second polymorphism of RAD 51 172 G/T, heterozygous variant GT genotype was associated with a 1.68 fold (OR=1.68; 95%CI=1.08-2.61; p<0.02) elevation when compared with controls. In the case of the Thr241Met polymorphism of XRCC3, we observed a 16 fold (OR=16; 95% CI=3.78-69.67; p<0.0002) increased HNC risk in patients compared to controls. These results further suggested that RAD51 (135G/C, 172 G/T) and XRCC3 (Thr241Met) polymorphisms may be effective biomarkers for genetic susceptibility to HNC. Larger studies are needed to confirm our findings and identify the underlying mechanisms.

• Proceedings of the Optical Society of Korea Conference
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• 1991.06a
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• pp.9-12
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• 1991

방전가열형 구리증기레이저의 비축형 불안정공진기를 사용하여 레이저의 빔발산각을 안정공진기의 경우에 비해 25배 (200 $\mu$rad) 까지 줄였다. 이때 불안정공진기의 배율 M 은 50 이었으며, 평균출력은 안정공진기를 사용할 경우에 비해 53 %를 얻었다. 또한, M이 8.5 일 때 (750 $\mu$rad) 평균출력은 안정공진기의 94% 까지 도달하였다.

• Toxicological Research
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• v.20 no.1
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• pp.21-29
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• 2004

We used cDNA microarray to assess gene expression profiles in hematopoetic cell line, U-937, exposed to low doses of ionizing irradiation. The 1,000 DNA elements on this array were PCR-amplified cDNAs selected from named human cancer related genes. According to the strength of irradiation, the levels of some gene expression were increased or decreased as dose-dependent manner. The gene expressions of Tubulin alpha, protein kinase, interferon-alpha, -beta, -omega receptor and ras homolog gene family H were significantly increased. Especially, Tubulin gene was shown 2.5 fold up-regulated manner under stress of 500 rad irradiation than 200 rad. On the other hand, fibroblast growth factor 12 and four and a half LIM domains, etc. were significantly down-regu-lated. Also, tumor protein 53(TP53) related genes that p53 inducible protein, tumor protein 53-binding protein looks of little significance as radiation sensitive manner. The radio-sensitivity of tubulin gene etc. that we proposed could be useful to rapid and correct survey for the bio-damage by exposure to low dose irradiation.

• Radiation Oncology Journal
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• v.4 no.2
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• pp.155-163
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• 1986

From Nov. 1983 through Feb. 1986, 35 patients of uterine cervical cancer were treated by external radiation therapy and intracavitary radiation therapy using Fletcher-Suit-Delclos applicator. Age of the patients ranged from 32 to 70 years (median age: 53 years). All patients had follow up from 9 to 34 months and median follow up of 20 months.4 patients were in stage I, 25 were in stage II, 5 were in stage III and 1 was in stage IV. Overall regression rate was $80\%$ and uncorrected actuarial 2 year survival rate was $88\%$. The incindence of rectal complications were analyzed. There was no rectal complication in the patients who received less than 7000 rad maximal rectal dose, but 2 out of 17 patients who received more than 7000 rad developed moderate degree (grade 2) of rectal complication. In viewing of our results, Fletcher-Suit-Delclos applicator (3M) seemed to be an appropriate instrument for intracaviary radiation therapy in the patients of uterine cervical cancer.

• Korean Journal of Digital Imaging in Medicine
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• v.7 no.1
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• pp.7-13
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• 2005

Purpose : Image plate (IP) is substituted for film in computed radiography. This study is to investigate into a variation of contrast and detail by the number used of image plate in computed radiography. Materials and Methods : A Contrast-Detail(CD)-RAD 2.0 phantom(Nijmegen hospital, The Netherlands) was used for this study. The computed radiography(CR) CD-RAD phantom images were acquired at 40 kVp, 160 mA, 1.6 mAs, and small focus with the Shimadzu general radiography UD-150B-10 system and Fuji FCR 5000 image process system with speed of 200. The IP used including once, 5000 times, and 10000 times also was used. The numerical value of image quality figures (IQF) was produced by CD-RAD analyser(the program is installed in the directory), and then contrast-detail curve was drawn. Results : In this study, the value of IQF was 3.53 in IP used once, 3.40 in 5000 times, and 3.22 in 10000 times. Conclusions : There was a variation of contrast-detail curve by the number used of IP with contrast-detail phantom in computed radiography. Therefore, it is necessary that the IP with lower IQF and a shift of contrast-detail curve to the lower left part is used.

• Journal of the Korean Society of Safety
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• v.33 no.4
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• pp.46-53
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• 2018

This study examined the maximum resistant moment and nonlinear rotational stiffness of wedge joint between the vertical and horizontal members of system supports. To examine the maximum resistant moment and propose the nonlinear rotation stiffness of wedge joint, 6 specimens were tested and additional 3 specimens, where the horizontal member was welded to the vertical member, were tested to compare the moment capacity of wedge joints. The average maximum moment in the tested wedge joint was 1.183 kNm which represented about 70 % of the maximum moment developed in the welded specimens. And, as simulating nonlinear rotational stiffness of the wedge joint, a tri-linear model was suggested. The rotational stiffness was estimated as 23.095 kNm/rad in first stage, 7.945 kNm/rad in second stage, and 3.073 kNm/rad in third stage. For the failure mode, the specimen with the wedge joint showed the failure of joint between vertical and horizontal members. However, the specimen with welded joint represented the yielding of horizontal members.