• Title/Summary/Keyword: RABITS

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Purification of Anti-ox Red Blood Cell IgG Antibody for T subset Assay (T subset정량(定量)을 위한 항우적혈구(抗牛赤血球) IgG항체(抗體)의 분리.정제(分離.精製)(I))

  • Ha, Youn-Mun;Lee, Jean-Yong;Lim, Soo-Duk
    • The Journal of the Korean Society for Microbiology
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    • v.15 no.1
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    • pp.71-75
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    • 1980
  • Antisera to ox red blood cell were prepared by intraperitoneal hypermultiple injections without adjuvant in outbred white rabits. Purified IgG fraction from these rabbits anti-ox red blood cell antiserum for T subset assay was obtained by precipitation with 50% saturated ammonium sulphate followed by DEAE-cellulose chromatography and Sephadex G-150 gel filtration. These purified IgG fraction was compared with Cappel company standard IgG fraction for $T_G$ subpopulation assay. We used home-made IgG fraction and obtained favorable results in $T_G$ subopulation assay as Cappel company standard IgG fraction.

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A Comparative Study About the Effect of Nonablative Laser and Thermal energy on Rabbit Knee Joint Capsular Properties (비융해성 LASER 및 열에너지의 가토 슬관절막에 대한 영향 비교)

  • Byun Ki-Yong;Rhee Kwang-Jin;Lee Jun-Ho
    • Clinics in Shoulder and Elbow
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    • v.4 no.1
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    • pp.1-12
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    • 2001
  • Purpose: To evaluate the histologic effect(LM and EM findings)of nonablative LASER and thermal energy on knee joint capsule of rabbit. Material and Methods: The nonablative LASER and thermal energy was applied to the rabits(average age 36 weeks, weight 5 ㎏). There were divided into 4 groups with 6 rabbits in each. The group I received 6 watts of LASER, group Ⅱ 12 watts of LASER, group Ⅲ 60° of thermal energy, and group Ⅳ 70° of thermal energy. The histologic study included H-E, Massons trichrome stain and electron microscopy at immediate, 3 weeks and 6 weeks after operation. Results: The histologic finding in immediate after operation was shown a fibrous degeneration of collagen on all groups and related to the energy level. The histologic finding after 3 weeks showed fibrosis and this fibrosis related the level of energy. Especially the group IV was shown flattening of capsule and deep fibrosis. The histologic finding after 6 weeks was shown marked recovery of collagen arrangement and capillary proliferation in group Ⅰ,Ⅱ and Ⅲ. But in the group Ⅳ not recovered. Conclusion: The nonablative LASER or thermal energy can cause degeneration, fibrosis and contracture of joint capsular collagen.

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