• Title/Summary/Keyword: R-T Characteristics

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Complete genome sequence of Marinobacter salarius HL2708#2 isolated from a lava sea water environment on Jeju Island (제주용암 해수 환경에서 분리한 Marinobacter salarius HL2708#2의 유전체 해독)

  • Oh, Hyun-Myung;Kim, Dae-Hyun;Han, Seong-Jeong;Song, Jong-Ho;Kim, Kukhyun;Jang, Dongil
    • Korean Journal of Microbiology
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    • v.55 no.1
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    • pp.69-73
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    • 2019
  • During screening of microbes for compounds having cosmetic benefits, we isolated Marinobacter salarius HL2708#2 from lava seawater on Jeju Island, Republic of Korea. The complete genome sequence was determined. Strain HL27080#2 features a circular chromosome of 4,304,603 bp with 57.21% G+C content and a 244,163 bp plasmid with 53.14% G+C. There were 4,180 protein coding sequences identified, along with 49 transfer RNA and 18 ribosomal RNA noncoding genes. The genome harbored genes for the utilization of alcohol, maltose/starch, and monosaccharide as sole carbon sources. Genes responsible for halophilic characteristics and heavy metal resistance could be annotated, as well as aromatic and alkane hydrocarbons. Contrary to the prior report that M. salarius is negative for nitrate and nitrite reduction, nitrate/nitrite reductase along with nitrate/nitrate transporters and nitronate monooxygenase were evident, suggesting that strain HL2708#2 may be able to denitrify extracellular nitroalkenes to ammonia.

Genome sequence of Actinomyces georgiae KHUD_A1 isolated from dental plaque of Korean elderly woman (한국 노인 여성의 치태에서 분리된 Actinomyces georgiae KHUD_A1의 유전체 염기서열 해독)

  • Moon, Ji-Hoi;Shin, Seung-Yun;Hong, Won Young;Jang, Eun-Young;Yang, Seok Bin;Ryu, Jae-In;Lee, Jin-Yong;Lee, Jae-Hyung
    • Korean Journal of Microbiology
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    • v.55 no.1
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    • pp.74-76
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    • 2019
  • Gram-positive anaerobic bacilli Actinomyces spp. commonly reside on mucosal surfaces of the oropharynx, gastrointestinal tract, and urogenital tract. Here, we first report the draft genome sequence of Actinomyces georgiae KHUD_A1, isolated from dental plaque of a Korean elderly woman. The genome is 2,652,059 bp in length and has a GC content of 68.06%. The genome includes 2,242 protein-coding genes, 9 rRNAs, and 64 tRNA. We identified 157 KHUD_A1 strain-specific genes, including genes encoding CPBP family intramembrane metalloprotease, bile acid: sodium symporter family protein, Txe/YoeB family addiction module toxin and Phd/YefM family antitoxin. The sequence information of A. georgiae KHUD_A1 will help understand the general characteristics of the bacterial species and the genome diversity of the genus Actinomyces.

A Study on the Dimensional Stabilization of Domestic Small-Diameter Logs by Polyethylene Glycol Treatment - Comparison of Ring-Porous Wood and Diffuse-Porous Wood - (Polyethylene glycol(PEG)처리에 의한 국내산 소경재의 치수안정화에 관한 연구II - 환경재와 산공재의 비교 -)

  • Kwon, Goo-Joong;Kwon, Sung-Min;Kim, Nam-Hun
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.4
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    • pp.8-15
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    • 2003
  • This study was carried out to investigate the dimensional stability of wood treated with PEG for better utilization of domestic small-diameter logs. Four species of ring- porous wood(Quercus mongolica FISCH, Quercus variabilis BLUME, Fraxinus rhynchophylla HANCE, Paulownia coreana UYEKI,) and four species of diffuse-porous wood(Prunus sargentii REHDER, Betula davurica PALL, Populus tomentiglandulosa T. LEE, Cornus controversa HEMSLEY) were used for this experiment. The shrinkage of wood decreased with increasing the concentration of PEG. The shrinkage of diffuse- porous woods was lower than that of ring-porous woods. The weight gain increased in proportional to the PEG concentration. Diffuse-porous woods showed a little higher weight gain than ring-porous woods. Bulking effect also increased with increasing the PEG concentration, but was in inverse proportional to the molecular weight of PEG. Diffuse-porous woods showed higher bulking effect than ring-porous woods. Consequently, diffuse-porous woods showed better dimensional stability than ring- porous woods. It was considered that dimensional stability was affected by characteristics of wood such as vessel and tylosis, and density.

A Study on Optimal Reinforcing Type of Precast Retaining Wall Reinforced by Micropiles (마이크로파일로 보강된 프리캐스트 콘크리트 옹벽의 최적보강형태에 관한 연구)

  • Kim, Hong-Taek;Park, Jun-Yong;Yoo, Chan-Ho
    • Journal of the Korean Geotechnical Society
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    • v.22 no.11
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    • pp.89-99
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    • 2006
  • The PCRW (Precast Concrete Retaining Wall) has many advantages compared with cast in place concrete retaining wall : shorter construction period, excellency of quality and minimum interference with the adjacent structure and traffics. However, shallow foundation type of PCRW, which has comparatively better ground condition, has some disadvantages such as difficulty in transportation and higher cost due to the size of PCRW being expanded by resisting only with self-weight if there is no other supplementary reinforcement. The presented study, in order to complement such disadvantages of PCRW, have applied the micropile method. The micropile method has advantages like low-cost and high-efficiency and does not require huge space, because it can be executed with small size equipment. However, the mechanical behavior characteristics of the PCRW reinforced by micropile, which is installed to improve the reinforcement effect, is not yet clearly identified and there is no suggested standard as to the length, diameter, install angle and install position of micropiles. Hence, this method is yet being designed depend on engineer's experience. In this study, various laboratory model tests as to sliding and overturning were performed in order to identify and present the optimum type of reinforcement and reinforcement effect of the PCRW reinforced by micropiles. In addition, it also executed numerical analysis for the purpose of verifying the optimum type of reinforcement for micropiles based on the results of laboratory model tests. The optimum reinforcement type of micropiles was estimated by model test and numerical analysis. The length of micropiles is 0.4 times wall height and the diameter is 0.04 times wall length.

Relationships among Organizational Commitment, Job Satisfaction, and Learning Organization Culture in One Korean Private Organization

  • LIM, Taejo
    • Educational Technology International
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    • v.8 no.1
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    • pp.17-39
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    • 2007
  • The purpose of this study was to identify the relationships among organizational commitment, job satisfaction, and learning organization culture. This study was conducted in five sub-organizations of one Korean conglomerate company. One thousand employees were randomly and proportionately selected, with 669 useable cases obtained, for a response rate of 67%. The organizational commitment instrument used from the "affective, continuance, and normative commitment" scale (ACNCS) of Allen and Meyer (1990). The "Dimensions of Learning Organization Questionnaire" of Watkins and Marsick (1997) was used to measure learning organization culture. The short form MSQ (Minnesota Satisfaction Questionnaire), developed by Weiss, Dawis, England, and Lofquist (1967), was the third instrument used. Descriptive statistics, correlational statistics, and inferential statistics (ANOVA and t-tests) were used. Organizational commitment (except for continuance) is moderately and positively related to job satisfaction and moderately and positively related to learning organization culture. In addition, learning organization culture is weakly to moderately and positively related to job satisfaction. No differences by age were found. Four-year college graduates are more likely to have higher creating continuous learning opportunities in learning organization culture than graduate school degree holders. Males are likely to have higher affective and continuance organizational commitment than females. Employees working in R&D, Engineering, and Manufacturing (REM) are likely to have higher continuance organizational commitment than do other types of jobs. Employees are more likely to have higher learning organization culture and job satisfaction than assistant managers. Assistant managers have higher continuance organizational commitment than managers. Managers generally have higher organizational commitment, learning organization culture, job satisfaction than assistant managers. They also have higher learning organization culture than employees. Employees who had worked for less than four years in their current job and organization have higher promoting inquiry and dialogue in learning organization culture than those who have worked for ten years or more. Employees in the insurance organization have higher affective organizational commitment, learning organization culture, and job satisfaction than those of the other organizations. Finally, employees of the electronic company have higher continuance organizational commitment in learning organization culture than those of other companies. In summary, this research enables CEOs and HRD and HRM practitioners to view organizational commitment, learning organization culture, and job satisfaction as important variables in exploring diverse ranges of topics related to the workplace. And then, they can diversely apply their management, interventions and practices to fit these diverse characteristics.

Analysis of whole genome sequencing and virulence factors of Vibrio vulnificus 1908-10 isolated from sea water at Gadeok island coast

  • Hee-kyung Oh;Nameun Kim;Do-Hyung Kim;Hye-Young Shin;Eun-Woo Lee;Sung-Hwan Eom;Young-Mog Kim
    • Fisheries and Aquatic Sciences
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    • v.26 no.9
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    • pp.558-568
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    • 2023
  • Vibrio vulnificus is an aquatic bacterium causing septicemia and wound infection in humans. To understand this pathogen at the genomic level, it was performed whole genome sequencing of a cefoxitin-resistant strain, V. vulnificus 1908-10 possessing virulence-related genes (vvhA, viuB, and vcgC) isolated from Gadeok island coastal seawater in South Korea. The genome of V. vulnificus 1908-10 consisted of two circular contigs and no plasmid. The total genome size was estimated to be 5,018,425 bp with a guanine-cytosine (GC) content of 46.9%. We found 119 tRNA and 34 rRNA genes respectively in the genome, along with 4,352 predicted protein sequences. Virulence factor (VF) analysis further revealed that V. vulnificus 1908-10 possess various virulence genes in classes of adherence, antiphagocytosis, chemotaxis and motility, iron uptake, quorum sensing, secretion system, and toxin. In the comparison of the presence/absence of virulence genes, V. vulnificus 1908-10 had fur, hlyU, luxS, ompU, pilA, pilF, rtxA, rtxC, and vvhA. Of the 30 V. vulnificus comparative strains, 80% of the C-genotype strains have all of these genes, whereas 40% of the E-genotype strains have all of them. In particular, pilA were identified in 80% of the C-type strains and 40% of the E-type strains, showing more difference than other genes. Therefore, V. vulnificus 1908-10 had similar VF characteristics to those of type C strains. Multifunctional-autoprocessing repeats-in-toxin (MARTX) toxin of V. vulnificus 1908-10 contained 8 A-type repeats (GXXGXXXXXG), 25 B.1-type repeats (TXVGXGXX), 18 B2-type repeats (GGXGXDXXX), and 7 C-type repeats (GGXGXDXXX). The National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) showed that the RtxA protein of V. vulnificus 1908-10 had the effector domain in the order of cross-liking domain (ACD)-C58_PaToxP-like domain- α/β hydrolase-C58_PaToxP-like domain.

Evaluation of Growth Characteristics of T hree Monocot Plants Native to Korea according to Cultivation Environments in Greenhouse (온실재배환경에 따른 자생 단자엽식물 3종의 생육 특성 평가)

  • Yu-Jin Kim;Hamin Lee;Boran Ji;Kichan Kim;Bo-Kook Jang;Cheol Hee Lee;Ju-Sung Cho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.47-47
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    • 2022
  • 단자엽식물은 잎의 곡선과 직선의 조화가 아름다워 관상용으로 가치가 높은 식물이다. 이로 인해 자생 단자엽식물의 실내 관상 및 조경용 소재개발 연구가 활성화되고 있으나 재배에 대한 정보는 미비한 실정이다. 본 연구에서는 국내 자생 단자엽식물인 참김의털[Festuca ovina L. var. coreana (St.-Yves) St.-Yves], 청사초(Carex breviculmis R.Br.), 골풀[Juncus decipiens (Buchenau) Nakai]을 대상으로 몇 가지 재배 조건에 대한 생육 차이를 확인하였다. 국립생물자원관으로부터 분양 받은 세 종의 종자는 휴면 여부와 발아 적온을 확인하기 위해 종자를 페트리디쉬에 파종한 후 15, 20, 25, 30℃(명 또는 암조건) 및 25/15℃(16/8h, 명/암) 조건에 배치하였다. 재배실험은 유리온실에서 수행되었으며, 토양에 파종 후 4주간 육묘한 다음 균일한 생육상태의 묘를 7cm 포트에 이식하여 실험의 재료로 사용하였다. 토양실험은 시판 원예용상토와 마사토를 혼합하여 사용하였으며, 비율을 1:1, 2:1 및 3:1로 조성하였다. 차광 환경은 55, 75% 차광과 무차광 조건으로 달리 하였으며, 추비는 재배 6, 8, 10주차에 500, 1,000ppm의 하이포넥스(NPK 7-10-6) 용액을 엽면시비 하였다. 토양실험을 제외한 모든처리구에서는 원예상토 단용을 사용하였으며, 이식 후 12주간 재배하였다. 발아 실험 결과, 참김의털은 명조건에서 25/15와 15℃를 제외하고 발아율이 10% 이하로 조사되었으며, 암조건에서 온도가 증가할수록 발아율이 감소하였다. 청사초는 모든 온도에서 90%이상 발아하였으며, 암조건의 경우 발아율이 10% 이하로 조사되어 광발아성 종자로 확인되었다. 골풀은 실험이 진행된 30일 동안 모든온도에서 발아하지 않았으나, 재배실험을 위해 토양에 파종된 골풀은 파종 약3주 이후 발아가 관찰되었다. 재배실험 결과, 참김의털과 청사초의 경우 2:1로 혼합한 토양에서 엽수와 분얼수, 생체중 등이 가장 높게 조사되어 관상적 가치가 높았으며, 골풀은 3:1의 혼합토양에서 생육이 가장 우수하였다. 차광은 세 종 모두에게서 생육에 부정적인 영향을 미쳤으며, 청사초와 골풀은 500ppm의 하이포넥스를 시비하였을 때 생육이 촉진되었다.

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Characteristics of Seedling Quality of Rhus javanica L. 1-year-old Container Seedling by Fertilization Level (붉나무 1년생 용기묘의 시비수준별 묘목품질 특성)

  • Yang Soo Kim;Sang Geun Kim;Jung Suk Um;Geun Sik Kim;Ki Seon Song;Jae Sun Yi
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.74-74
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    • 2022
  • 본 연구는 붉나무의 기능성 성분의 산업화 활용을 위한 고품질의 붉나무 1년생 용기묘 대량생산을 위해 실시되었다. 붉나무 1년생 용기묘의 묘목품질의 향상을 위해 다양한 농도의 시비처리하여 우량묘 생산에 적합한 시비수준을 구명하고자 하였다. 시비실험은 무시비구 포함하여 수용성 복합비료 (N:P:K=20:20:20, v/v)를 1,000mg·L-1, 2,000mg·L-1, 3,000mg·L-1 수준으로 실시하였다. 시비처리는 붉나무 용기묘의 간장과 근원경 생장은 1,000mg·L-1 시비처리구에서 생장이 가장 좋게 나타났으며 시비수준이 증가할수록 감소하는 경향을 보였다. 뿌리형태 특성분석에서도 전체뿌리길이, 투영단면적, 표면적 및 뿌리부피가 간장과 근원경 생장과 동일한 경향을 보이는 것으로 조사되었다. 건물 생산량은 부위별 잎, 줄기, 뿌리 및 전체가 모두 1,000mg·L-1 시비처리구에서 가장 높았으며, 그 이상의 시비수준에서는 점점 감소하는 경향을 보였다. H/D(Hight/Root collar diameter)율은 전체가 3.82(무시비구) ~ 4.90, T/R(Top/Root)율은 전체가 0.56(1,000mg·L-1 시비처리구) ~ 0.82로 조사되었다. 시비처리 에 의한 붉나무의 LWR(Leaf dry weight ratio)은 무시비구, SWR(Shoot dry weight ratio)은 3,000mg·L-1 시비처리구, 그리고 RWR(Root dry weight ratio)은 1,000mg·L-1 시비처리구에서 유의적 차이를 보이며 높게 나타났다. 묘목품질지수(QI)의 경우 1,000mg·L-1 시비처리구에서 12.13으로 다른 시비수준들보다 월등히 높았다. 본 실험의 결과를 종합하면, 뿌리발달이 좋은 우량한 붉나무 생산에 적절한 시비수준은 1,000mg·L-1 정도인 것으로 판단된다.

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A study on knowledge, self-efficacy and compliance in Reumatic arthritis Patients (류마티스 관절염 환자의 지식, 자기효능감 및 치료이행과의 관계연구)

  • Kim, Soon-Bong
    • Journal of muscle and joint health
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    • v.5 no.2
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    • pp.238-252
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    • 1998
  • Reumatic arthritis is a disease with joint pain being one of the key symptoms. The patient suffers from the pain, stiff sensation and edema due to the inflammation taking Place In one or more joints. Accompanying these problems are fatigue, unusual exhaustion, fever, tachycardia and weakness. Inaddition, joints are often deformed and muscles shrink along with the progress of edema, coupled with depression and psychological instability resulting from the loss of the mobile function and limitations on the daily life. Some patients become fed up with the long and hard flight with the disease and just give up, which aggravates the symptoms. Others come to the hospital only when the conditions have become serious. We need to prevent these and guide the patients in the right direction. Against this backdrop, this study aims to look into the relations between the knowledge on the part of the patients together with their feeling of self-efficacy and the compliance. The results are expected to help the patients improve their life, In addition to providing useful materials for setting up appropriate plan for nursing intervention. The study was conducted by distributing questionnaire to 88 patients selected from the out-patient department of a university hospital in Inchon, from April 6 to 27, 1998. The following tools were used the yardstick of self-efficacy, developed in 1997 by the Society for the Health of Rheumatism Patients, was used for measuring the levels of knowledge and the feeling of self-efficacy. The degree of compliance was measured by the data collected from documents in addition to the results of the analysis of the interviews with the patients. The reliability of the tools was confirmed. In the analysis, the general characteristics were expressed in figures and percentages. The levels of knowledge, feeling of self-efficacy, and compliance were expressed in the average values and standard deviations. The relations among the variables following the general characteristics were analysed by the t-test and one-way ANOVA. The Pearson correction coefficient was used for the analysis of factors. Multiple-loop analysis was used to identify the variables affecting the compliance. The following are the results of this study. 1. Among the 88 patients, 18 were men and the remaining 70 were women, with a ratio 1 : 3.87. Regarding the age groups, 23 were between 50 and 59 years old, with those between 50 and 69 accounting for 51.1% of the total. High school graduates or higher amounted to 58%. Religious patients was 67% or 59 persons. Fifty nine percent were unemployed, and 58.3% (49 persons) had two children or fewer. The period of suffering from rheumatism varied between 2 months and IS years, with 70% less than years. 2. The average figure In relation to the of knowledge was 17.63 points over 30 or 58. 76%, which means a medium level. 3. The average figure of the feeling of self-efficacy was 60.06 points. 4. The level of compliance was 3.26, which was above average. 5. The relation between the feeling of self-efficacy and compliance showed an "r" value of 0.37, which was significant. It means that the higher the feeling, the greater the compliance points. 6. The analysis of the knowledge level revealed that the difference is found only between the college graduates and junior-high graduates or lower. 7. The feeling of self-efficacy varied along with the age and education level. 8. The general characteristics of patients as discussed above did not show significant difference with the compliance. 9. Regarding the elements influencing the compliance, the number of children, period of suffering, income, age, feering of self-efficacy, knowledge, and compliance had 54% of significance. In conclusion, rheumatism victims can lead a better life if they are appropriately educated, based on efficient training program from the early days of the disease ; if they become able to manage themselves thanks to the training ; and if they are helped by a program focusing on the increase of the feeling of self-efficacy aimed at changing patient's behavior.

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Effects of CLA-vegetable Oils and CLA-lard Additives on Quality Characteristics of Emulsion-type Sausage (유화형 Sausage의 품질특성에 식물성유와 동물성유 CLA 첨가가 미치는 영향)

  • Lee, J. I.;Lee, J. H.;Kwack, S. C.;Ha, Y. J.;Jung, J. D.;Lee, J. W.;Lee, J. R.;Joo, S. T.;Park, G. B.
    • Journal of Animal Science and Technology
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    • v.45 no.2
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    • pp.283-296
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    • 2003
  • Emulsion-type sausages were manufactured to investigate the effects of CLA-vegetable oils and CLA-lard on quality characteristics of emulsion-type sausage. Each treatments replaced pork back fat with CLA-corn oil (CLA-CO), CLA-safflower seed oil (CLA-SSO) and CLA-lard (CLA-LD) were stored during 1, 7, 14, 21 and 28 days at 4$^{\circ}C$. The changes in physico-chemical properties, texture, thiobarbituric acid reactive substances (TBARS), fatty acid composition and CLA content of each treatments were measured during 1, 7, 14, 21 and 28 days at 4$^{\circ}C$. During storage, pH of control showed higher pH value than that of CLA-vegetable and CLA-lard treatments. The pH values of all treatments was significantly low at storage 14 days (p<0.05). The color L* and a*-value of all the treatments was not significantly changed with the storage periods. Color b*-value of CLA-treatments was higher than that of control during the storage period. Fatty acids composition was changed by addition of CLA-vegetable oils and CLA-lard. All kinds of fatty acids composition were decreased with CLA replacements than that of the control. Whereas linolenic acid content extremely increased by replacement of CLA-vegetable. CLA content of CLA-treatments was extremely increased than that of the control. The cohesiveness, springness, gumminess and brittleness of CLA-LD treatment was significantly higher (P<0.05) than that of the other treatments. The TBARS value of all treatments were significantly increased (P<0.05) as the storage period was passed. TBARS of sausage products containing CLA-CO was the lowest. Summing up the a forementioned results, emulsion-type sausage manufacturing with CLA-vegetable oil and CLA-lard was not affected in physico-chemical properties and texture characteristics. Also, it may be assumed that the high quality sausage can be manufactured with the extent of storage period and CLA accumulation.