• The Korean Journal of Ecology
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• v.20 no.6
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• pp.413-421
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• 1997

Ecological characteristics of Rumex acetosella have been studied both in the laboratory and in the field. R. acetosella grows well straight up after germination, elongating and thickening their rhizomes with sprouts erupting along their bodies of the rhizome. The number and development of leaves and ramets reach their peak by April. The size of leaves and the cover degree of the plant increase from April to May. R. acetosella is an ephemeral, a short life mode plant, finishing its life cycle in July or August. The germination and seedling growth of selected species exposed to aqueous extracts of R. acetosella were in inverse proportion to concentration. The growth of Rumex japonicus and Digitaria sanguinalis cultivated in soil with R. acetosella was more inhibited than that of the control. Twelve chemical compounds were identified from R. acetosella by GC and HPLC and verified by bioassay with the same chemical reagents. It would be assdumed that these chemical substances are responsible for the allelopathic effect of R. acetosella. In short, R. acetosella plants grow vigorously from April and maintain their superior competitive ability to other plants in forming their communities by emitting chemical substances into their environment.

• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.244-252
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• 2007

Escherichia coli has a PhoR-PhoB two-component regulatory system to detect and respond to the changes of environmental phosphate concentration. For the E. coli W3110 strain growing under phosphate-limiting condition, the changes of global gene expression levels were investigated by using DNA microarray analysis. The expression levels of some genes that are involved in phosphate metabolism were increased as phosphate became limited, whereas those of the genes involved in ribosomal protein or amino acid metabolism were decreased, owing to the stationary phase response. The upregulated genes could be divided into temporarily and permanently inducible genes by phosphate starvation. At the peak point showing the highest expression levels of the phoB and phoR genes under phosphate-limiting condition, the phoB- and/or phoR-dependent regulatory mechanisms were investigated in detail by comparing the gene expression levels among the wild-type and phoB and/or phoR mutant strains. Overall, the phoB mutation was epistatic over the phoR mutation. It was found that PhoBR and PhoB were responsible for the upregulation of the phosphonate or glycerol phosphate metabolism and high-affinity phosphate transport system, respectively. These results show the complex regulation by the PhoR-PhoB two-component regulatory system in E. coli.

• Water Engineering Research
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• v.2 no.4
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• pp.219-229
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• 2001

Synthetic unit hydrograph equations for rainfall run-off characteristics analysis and estimation of design flood have long and quite frequently been presented, the Snyder and SCS synthetic unit hydrograph. The major inputs to the Snyder and SCS synthetic unit hydrograph are lag time and peak coefficient. In this study, the methods for estimating lag time and peak coefficient for small watersheds proposed by Zhao and McEnroe(1999) were applied to the Kum river basin in Korea. We investigated lag times of relatively small watersheds in the Kum river basin in Korea. For this investigation the recent rainfall and stream flow data for 10 relatively small watersheds with drainage areas ranging from 134 to 902 square kilometers were gathered and used. 250 flood flow events were identified along the way, and the lag time for the flood events was determined by using the rainfall and stream flow data. Lag time is closely related with the basin characteristics of a given drainage area such as channel length, channel slope, and drainage area. A regression analysis was conducted to relate lag time to the watershed characteristics. The resulting regression model is as shown below: ※ see full text (equations) In the model, Tlag is the lag time in hours, Lc is the length of the main river in kilometers and Se is the equivalent channel slope of the main channel. The coefficient of determinations (r$^2$)expressed in the regression equation is 0.846. The peak coefficient is not correlated significantly with any of the watershed characteristics. We recommend a peak coefficient of 0.60 as input to the Snyder unit-hydrograph model for the ungauged Kum river watersheds

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.4
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• pp.439-447
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• 1996

Monthly test day production for 12,020 records, were collected from six of the largest specialized dairy farms located in central region of the Kingdom of Saudi Arabia. The records described lactating cows in four parities and two seasons of calving. Monthly test day records were fitted using Wood's model $At{{^b}{_e}}^{-ct}$ with multiple and additive error term. Linear and non-linear regression models were used to find the estimates of the parameters necessary to draw the lactation curves. The shape of the lactation curves of different parities showed that third lactation has the heighest peak (43.08 kg) for linear regression model and (42.08 kg) for non-linear regression model. Fourth lactation has the lowest peak (24.00kg) for linear regression model and (25.64 kg) for non-linear regression models. Cows of second and third lactations reached the peak at 58 day for both linear and non-linear regression models. Cows of first lactation were more persistent and had late peak at 68 and 67 days for both models respectively. While, third lactation cows were lower persistent and had early peak at 58 day for both models. Cows calved at winter months have higher starting values (A), higher ascending slope (b) and higher decending slope (c). Least square means of milk yield of the first four parities and for overall data were 6,653, 7,659, 7,482, 6,988 and 7,614 kg respectively. The corresponding lactation period were 358, 367, 350, 363 and 364 days respectively.

• Computers and Concrete
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• v.13 no.3
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• pp.411-421
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• 2014

A central pullout test was conducted to investigate the bonding properties between high strength rebar and reactive powder concrete (RPC), which covered ultimate pullout load, ultimate bonding stress, free end initial slip, free end slip at peak load, and load-slip curve characteristics. The effects of varying rebar buried length, thickness of protective layer and diameter of rebars on the bonding properties were studied, and how to determine the minimum thickness of protective layer and critical anchorage length was suggested according the test results. The results prove that: 1) Ultimate pull out load and free end initial slip load increases with increase in buried length, while ultimate bonding stress and slip corresponding to the peak load reduces. When buried length is increased from 3d to 4d(d is the diameter of rebar), after peak load, the load-slip curve descending segment declines faster, but later the load rises again exceeding the first peak load. When buried length reaches 5d, rebar pull fracture occurs. 2) As thickness of protective layer increases, the ultimate pull out load, ultimate bond stress, free end initial slip load and the slip corresponding to the peak load increase, and the descending section of the curve becomes gentle. The recommended minimum thickness of protective layer for plate type members should be the greater value between d and 10 mm, and for beams or columns the greater value between d and 15 mm. 3) Increasing the diameter of HRB500 rebars leads to a gentle slope in the descending segment of the pullout curve. 4) The bonding properties between high strength steel HRB500 and RPC is very good. The suggested buried length for test determining bonding strength between high strength rebars and RPC is 4d and a formula to calculate the critical anchorage length is established. The relationships between ultimate bonding stress and thickness of protective layer or the buried length was obtained.

• Biomedical Science Letters
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• v.8 no.4
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• pp.211-215
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• 2002

The baculovirus/Sf9 cell expression can be employed as a powerful system for producing large amounts of the human erythrocyte glucose transporter, GLUT1 heterologously In order to exploit the system further, it is necessary to develop a convenient method for demonstrating that the transporter expressed in insect cells is biologically active. To achieve this, we have expressed the human CLUT1 in insect cells and photolabelled the expressed protein with [$^3$H] cytochalasin B, a potent inhibitor of the human erythrocyte glucose transporter. Subsequently, the labelled proteins were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Membranes labelled with [$^3$H] cytochalasln B in the presence of L-Glucose yielded a single sharp peak of labelling of apparent $M_r$ 45,000 on SDS/polyacrylamide gels. The mobility of this peak corresponded exactly to that of the band detected by anti-glucose transporter antibodies on Western blots of membranes prepared from insect cells infected with recombinant virus. In addition, the sharpness of the radioactive peak provides further evidence for the conclusion that the expressed protein is much less heavily and heterogeneously glycosylated than its erythrocyte counterpart. No peak of labelling was seen with the membranes prepared from non-infected Sf9 cells. Furthermore, the incorporation of label into this peak was completely inhibited by the presence of 500 mM-D-Glucose during tile photolabelling procedure, showing the stereoselectivity of the labelling. These evidences clearly show that human glucose transporter expressed in insect cells exhibits native-like biological activity, and that photolabelling with [$^3$H] cytochalasin B can be a convenient means for analysing the biological activity of the transport protein expressed in insect cells.

• Analytical Science and Technology
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• v.15 no.4
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• pp.388-391
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• 2002

Rancidity of a soybean oil is investigated by an electrochmical method. The redox process of the soybean oil is totally irreversible and diffusion-controlled reaction. As scan rates are faster, the anodic peak potential of the soybean oil is shifted to the positive potential and the anodic current is increased. The anodic peak potentials of the soybean oil is not rarely changed up to open-42 days in the both atmosphere and room temperature. After the open times, the anodic peak potential is largely shifted to a negative direction. This indicates the oxidation of the soybean oil becomes easier. The anodic peak current of the soybean oil is decreased gradually up to open-42 days. But after the open times, the anodic peak current increases suddenly. It must be due to the formation of a carbonyl group owing to rancidity of the soybean oil.

• Journal of Welding and Joining
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• v.34 no.6
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• pp.55-61
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• 2016

Recently developed ferritic heat resistance steel, T24 was used to evaluate microstructure characteristics of simulated heat affected zone. Also, correlation between the prior austenite grain size and amount of $M_{23}C_6$ carbide dissolution was discussed. With the increasing of peak temperature, Grain size steadily increased up to $1050^{\circ}C$ and then rapidly increased at $1150^{\circ}C$. Of the peak temperature $950{\sim}1050^{\circ}C$, amounts of $M_{23}C_6$carbide dissolution are low. But Most of $M_{23}C_6$ carbide that is inhibited grain growth were dissolved above $1050^{\circ}C$ and decreased volume fraction of carbide. This indicates that grain growth may be achieved through dissolution of carbide in the base material. As of welding, due to very rapid heating rate, $M_{23}C_6$ carbide exists above equilibrium solution temperature that is $800^{\circ}C$, even at $1050^{\circ}C$. So, It was confirmed that close correlation between carbide dissolution in the base material and grain growth. Calculated grain size has a linear relationship with peak temperature, on the other hand, measured grain size discontinuously increased between $950{\sim}1050^{\circ}C$ and above $1050^{\circ}C$. Grain size of heat affected zone at $1350^{\circ}C$ peak temperature showed maximum 67um and minimum 4um. Also, The number of side showed 3 to 10.

• Journal of the Korea Organic Resources Recycling Association
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• v.8 no.2
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• pp.146-153
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• 2000

To elucidate succession of bacterial populations, especially nitrifying bacteria during the composting of cattle manure with apple pomace, fluorescent in situ hybridization(FISH) using rRNA targeted oligonucleotide probes were applied. The density of ammonia-oxidizing bacteria was ranged from $3,3{\times}10^6cells/g$ dw to $13,4{\times}10^6cells/g$ dw with the peak value after 26 composting days whereas that of nitrite-oxidizing bacteria varied between $6.0{\times}10^6cells/g$ dw and $17.2{\times}10^6cells/g$ dw with the peak value after 7 composting days. And the tendency that the numbers of nitrite-oxidizing bacteria were higher than those of ammonia-oxidizing bacteria, and the peak-time of their densities were the same as that of data determined by the ratio of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria to eubacteria. The peak of ammonia-oxidizing bacteria followed the peak of nitrite-oxidizing bacteria, at the late phase of composting process could be probably caused by the depletion of volatile ammonia of composting materials. Besides these results indicate that FISH method is a useful tool for detection of slow growing nitrifying bacteria.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.43 no.6
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• pp.775-784
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• 2023

In terms of flood management, it is necessary to analyze quantitative rainfall and runoff from a spatial and temporal perspective and to analyze runoff for heavy rainfall events that are concentrated within a short period of time. The simulation and analysis results of rainfall-runoff models vary depending on the type and input data. In particular, rainfall data is an important factor, so calculating areal mean rainfall is very important. In this study, the areal mean rainfall of the Samcheok Osipcheon(Riv.) watersheds located in the mountainous terrain was calculated using the Arithmetic Mean Method, Thiessen's Weighting Method, and the Isohyetal Method, and the rainfall-runoff results were compared by applying the distributional model S-RAT and the lumped model HEC-HMS. The results of the temporal transferability study showed that the combination of the distributional model and the Isohyetal Method had the best statistical performance with MAE of 64.62 m³/s, RMSE of 82.47 m³/s, and R² and NSE of 0.9383 and 0.8547, respectively. It is considered that this study was properly analyzed because the peak flood volume occurrence time of the observed and simulated flows is within 1 hour. Therefore, the results of this study can be used for frequency analysis in the future, which can be used to improve the accuracy of simulating peak flood volume and peak flood occurrence time in mountainous watersheds with steep slopes.