• Title/Summary/Keyword: R-MAT

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Morphological and Phylogenetic Characteristics of Tuber himalayense Collected from Rhizosphere of Quercus dentata in Korea

  • Park, Hyeok;Gwon, Ju-Hui;Lee, Jong-Chul;Kim, Hyun Suk;Seo, Geon-Sik;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.49 no.1
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    • pp.101-108
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    • 2021
  • We collected the ascomata of Tuber species from the rhizosphere of Quercus dentata in Danyang, Korea. We observed the morphological characteristics of ectomycorrhizal roots and ascomata, and identified the species based on the results of the phylogenetic analysis conducted using the DNA sequences of an internal transcribed spacer, a large-subunit rDNA, translation elongation factor 1-α DNA (TEF1), and MAT. Finally, we identified the fungal species as Tuber himalayense B.C. Zhang & Minter, which has not been recorded previously in Korea. We evaluated the morphological characteristics and conducted phylogenetic analysis of the ascoma and mycorrhiza (associated with Q. dentata) of T. himalayense.

Properties of Malonyl-CoA Decarboxylase from Rhizobium trifolii

  • An, Jae-Hyung;Lee, Gha-Young;Song, Jong-Hee;Lee, Dai-Woon;Kim, Yu-Sam
    • BMB Reports
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    • v.32 no.4
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    • pp.414-418
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    • 1999
  • A novel gene for malonyl-CoA decarboxylase was discovered in the mat operon, which encodes a set of genes involved in the malonate metabolism of Rhizobium trifolii (An and Kim, 1998). The subunit mass determined by SDS-PAGE was 53 kDa, which correspond to the deduced mass from the sequence data. The molecular mass of the native enzyme determined by field flow fractionation was 208 kDa, indicating that R. trifolii malonyl-CoA decarboxylase is homotetrameric. R. trifolii malonyl-CoA decarboxylase converted malonyl-CoA to acetyl-CoA with a specific activity of 100 unit/mg protein. Methylmalonyl-CoA was decarboxylated with a specific activity of 0.1 unit/mg protein. p-Chloromercuribenzoate inhibited this enzyme activity, suggesting that thiol group(s) is(are) essential for this enzyme catalysis. Database analysis showed that malonyl-CoA decarboxylase from R. trifolii shared 32.7% and 28.1% identity in amino acid sequence with those from goose and human, respectively, and it would be located in the cytoplasm. However, there is no sequence homology between this enzyme and that from Saccharopolyspora erythreus, suggesting that malonyl-CoA decarboxylases from human, goose, and R. trifolii are in the same class, whereas that from S. erythreus is in a different class or even a different enzyme, methylmalonyl-CoA decarboxylase. According to the homology analysis, Cys-214 among three cysteine residues in the enzyme was found in the homologous region, suggesting that the cysteine was located at or near the active site and plays a critical role in catalysis.

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Anti-Proliferative Effects of Dendrophthoe pentandra Methanol Extract on BCR/ABL-Positive and Imatinib-Resistant Leukemia Cell Lines

  • Zamani, Afiqah;Jusoh, Siti Asmaa Mat;Al-Jamal, Hamid Ali Nagi;Sul'ain, Mohd Dasuki;Johan, Muhammad Farid
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.11
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    • pp.4857-4861
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    • 2016
  • Background: Imatinib mesylate, a tyrosine kinase inhibitor specifically targeting the BCR/ABL fusion protein, induces hematological remission in patients with chronic myeloid leukemia (CML). However, the majority of CML patients treated with imatinib develop resistance with prolonged therapy. Dendrophthoe pentandra (L.) Miq. is a Malaysian mistletoe species that has been used as a traditional treatment for several ailments such as smallpox, ulcers, and cancers. Methods: We developed a resistant cell line (designated as K562R) by long-term co-culture of a BCR/ABL positive CML cell line, K562, with imatinib mesylate. We then investigated the anti-proliferative effects of D. pentandra methanol extract on parental K562 and resistant K562R cells. Trypan blue exclusion assays were performed to determine the IC50 concentration; apoptosis and cell cycle analysis were conducted by flow cytometry. Results: D. pentandra extract had greater anti-proliferative effects towards K562R ($IC50=192{\mu}g/mL$) compared to K562 ($500{\mu}g/mL$) cells. Upon treatment with D. pentandra extract at the IC50. concentration: K562 but not K562R demonstrated increase in apoptosis and cell cycle arrest in the G2/M phase. Conclusion: D. pentandra methanol extract exerts potent anti-proliferative effect on BCR/ABL positive K562 cells.

Evaluation of Pollutant Removal Efficiency through Field Test-Bed Experiment in the Rural Small Stream (저수지 유입하천 현장적용실험을 통한 수질정화효율 평가)

  • Choi, Sun Hwa;Oh, Jong Min;Kim, Tae-Hoon
    • Journal of Korea Water Resources Association
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    • v.47 no.12
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    • pp.1135-1143
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    • 2014
  • This study was carry out to evaluate of water purification in oxidation pond with filamentous algae mat. It is the water treatment process in the small rural streams to remove the organic materials and nutrients. We used the filamentous algae mat (FAM) which selectively predominate the filamentous algae to prevent the additional contamination by algae outflow, and we conducted a experiment on the water treatment process using the aquatic plants such as Eichhornia crassipes. The removal efficiencies (%) of water quality parameters were SS 80.9%, COD 74.6%, TN 76.8%, TP 84.4%, DTN 93.8% and DTP 98.3%, respectively. Temperature, a effect factor, was $21.8{\pm}5.9^{\circ}C$ during the operating period, according to temperature had no effect on the removal efficiencies of pollutants. Hydraulic retention time (HRT) strongly correlated with removal efficiencies (%) of SS and TP having r=0.414 (p<0.005), r=0.446 (p<0.005), respectively, and when HRT was 5day had highly removal efficiency (%) in SS and TP. TN and TP removal efficiency increased with ratio decreasing in both COD/TN and COD/TP of Influent.

Identification of 19 Species of Poisonous Plants from Jeju Island and Construction of a Database Using DNA-barcoding (DNA-barcoding을 이용한 제주도 자생 독성 식물 19종의 종 식별 및 데이터베이스 구축)

  • Kwon, Eunchae;Kim, Joo-Young;Chang, Miwha;Lee, Minji;Moon, Seohyun;Lee, Won-Hae
    • Korean Journal of Plant Resources
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    • v.35 no.2
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    • pp.346-361
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    • 2022
  • Food poisoning accidents caused by poisonous plants occur every year. As certain poisonous plants are mistaken for edible plants causing food poisoning, accurate species identification of poisonous plants is required. DNA barcodes suitable for species identification of poisonous plants and database that can be used for accurate species identification are necessary for their use in forensic cases. In this study, species identification of 19 poisonous plants native to Jeju Island using seven DNA barcodes (trnH-psbA, trnL-trnF, trnL intron, rbcL, matK, ITS1-ITS4, 18S rRNA) was performed to construct a database containing sequence information and DNA barcode universality. trnL-trnF barcode and ITS1-ITS4 barcode were the easiest markers for PCR amplification and sequence retrieval, and the combination of the two barcodes enabled single species identification in 18 out of 19 plants. Therefore, when an investigation of unknown poisonous plants is requested, combination of trnL-trnF and ITS1-ITS4 barcodes is considered as a primary marker for species identification. The database of recommended DNA barcodes for each poisonous plant presented in this study will be helpful in plants poisoning cases.

Case Study on 5kWp Transparent Thin-Film BIPV System (5kW급 투광형 박막 BIPV시스템의 실증연구)

  • An, Young-Sub;Kim, Sung-Tae;Lee, Sung-Jin;Song, Jong-Hwa;Hwang, Sang-Kun;Yoon, Jong-Ho
    • Journal of the Korean Solar Energy Society
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    • v.30 no.4
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    • pp.29-35
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    • 2010
  • This study has been carried out empirical research on Transparent Thin-film BIPV modules, BIPV modules installed on the exterior of the building are applied a laminated module 1kWp, double-glazing module 3kWp and triple-glazing module 1kWp. Applied to the total capacity of BIPV modules are 5kWp. In this study, design and construction process of BIPV systems is presented. In addition, through monitoring of the BIPV system, the temperature and the power characteristics of each module were analyzed. During the measurement period, the module temperature measurement results, the maximum surface temperature of $51.5^{\circ}C$ triple-glazing BIPV module showed the highest, followed by double-glazing BIPV module $49.1^{\circ}C$, $44.7^{\circ}C$ laminated modules, respectively. Power output results, the daily average double-layer modules showed 4.10kWh/day, triple-glazing module 1.57kWh, respectively 1.81kWh laminated modules. In particular, the power efficiency of triple-glazing BIPV module was lower than the power efficiency of the laminated BIPV module. This phenomenon is considered to be affected by the module temperature. In the future, BIPV modules in this study the relationship between module temperature and power characteristics plans to identify.

The Effect of Trunk Stabilization Exercise Using a Reformer on Trunk Control Ability, Balance, and Gait Function in Chronic Stroke Patients (리포머를 이용한 체간 안정화 운동이 만성 뇌졸중 환자의 체간 조절 능력과 균형 및 보행 기능에 미치는 영향)

  • Sang-Yong Han;Seong-Ho Jo;Dong-Hwan Park
    • PNF and Movement
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    • v.22 no.2
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    • pp.201-211
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    • 2024
  • Purpose: This study aimed to evaluate the effects of trunk stabilization exercises using a Reformer on trunk control, balance ability, and gait function in chronic stroke patients. Methods: The participants were 24 chronic stroke patients, randomly divided into two groups: trunk stabilization exercise using the Reformer group (TS-R, n = 12) and general trunk stabilization exercise group (GT-E, n = 12). Assessment methods included the Trunk Impairment Scale for trunk control, the AMTI force platform for static balance, the Timed Up and Go test for dynamic balance, and the Dynamic Gait Index for gait function. Assessments were conducted before and after the intervention. The intervention for the TS-R group consisted of bridging exercises using a Reformer, while the GT-E group performed bridging exercises on a mat. All interventions were performed for 17 minutes per session, five times a week, for a total of 20 sessions over four weeks. Statistical analysis was performed using repeated-measures ANOVA to analyze the interaction between groups and time. Results: The results of the repeated measures ANOVA indicated a significant interaction between the groups and time. The TS-R group showed statistically significant differences in all variables before and after the intervention. In contrast, the GT-E group did not show statistically significant differences in any variables before and after the intervention. Conclusion: The findings of this study suggest that trunk stabilization exercises using a reformer are effective in improving trunk control, balance ability, and gait function in chronic stroke patients.

Drainage Performance of Various Subsurface Drain Materials- (배수개선공법개발에 관한 연구(I) -각종 지하배수용 암거재료의 배수성능-)

  • 김철회;이근후;유시조;서원명
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.21 no.3
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    • pp.104-120
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    • 1979
  • I. Title of the Study Studies on the Development of Improved Subsurface Drainage Methods. -Drainage Performance of Various Subsurface Drain Materials- II. Object of the Study Studies were carried out to select the drain material having the highest performance of drainage; And to develop the water budget model which is necessary for the planning of the drainage project and the establishment of water management standards in the water-logged paddy field. III. Content and Scope of the Study 1. The experiment was carried out in the laboratory by using a sand tank model. The drainage performance of various drain materials was compared evaluated. 2. A water budget model was established. Various parameters necessary for the model were investigated by analyzing existing data and measured data from the experimental field. The adaptability of the model was evaluated by comparing the estimated values to the field data. IV. Results and Recommendations 1. A corrugated tube enveloped with gravel or mat showed the highest drainage performance among the eight materials submmitted for the experiment. 2. The drainage performance of the long cement tile(50 cm long) was higher than that of the short cement tile(25 cm long). 3. Rice bran was superior to gravel in its' drain performance. 4. No difference was shown between a grave envelope and a P.V.C. wool mat in their performance of drainage. Continues investigation is needed to clarify the envelope performance. 5. All the results described above were obtained from the laboratory tests. A field test is recommended to confirm the results obtained. 6. As a water balance model of a given soil profile, the soil moisture depletion D, could be represented as follows; $$D=\Sigma\limit_{t=1}^{n}(Et-R_{\ell}-I+W_d)..........(17)$$ 7. Among the various empirical formulae for potential evapotranspiration, Penman's formular was best fit to the data observed with the evaporation pans in Jinju area. High degree of positive correlation between Penman;s predicted data and observed data was confirmed. The regression equation was Y=1.4X-22.86, where Y represents evaporation rate from small pan, in mm/100 days, and X represents potential evapotranspiration rate estimated by Penman's formular. The coefficient of correlation was r=0.94.** 8. To estimate evapotranspiration in the field, the consumptive use coefficient, Kc, was introduced. Kc was defined by the function of the characteristics of the crop soil as follows; $Kc=Kco{\cdot}Ka+Ks..........(20)$ where, Kco, Ka ans Ks represents the crop coefficient, the soil moisture coefficient, and the correction coefficient, respectively. The value of Kco and Ka was obtained from the Fig.16 and the Fig.17, respectively. And, if $Kco{\cdot}Ka{\geq}1.0,$ then Ks=0, otherwise, Ks value was estimated by using the relation; $Ks=1-Kco{\cdot}Ka$. 9. Into type formular, $r_t=\frac{R_{24}}{24}(\frac{b}{\sqrt{t}+a})$, was the best fit one to estimate the probable rainfall intensity when daily rainfall and rainfall durations are given as input data, The coefficient a and b are shown on the Table 16. 10. Japanese type formular, $I_t=\frac{b}{\sqrt{t}+a}$, was the best fit one to estimate the probable rainfall intensity when the rainfall duration only was given. The coefficient a and b are shown on the Table 17. 11. Effective rainfall, Re, was estimated by using following relationships; Re=D, if $R-D\geq}0$, otherwise, Re=R. 12. The difference of rainfall amount from soil moisture depletion was considered as the amount of drainage required. In this case, when Wd=O, Equation 24 was used, otherwise two to three days of lag time was considered and correction was made by use of storage coefficient. 13. To evaluate the model, measured data and estimated data was compared, and relative error was computed. 5.5 percent The relative error was 5.5 percent. 14. By considering the water budget in Jinju area, it was shown that the evaporation amount was greater than the rainfall during period of October to March in next year. This was the behind reasonning that the improvement of surface drainage system is needed in Jinju area.

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THE INFLUENCE OF THE VITAL BLEACHING AGENTS ON TRANSLUCENCY OF THE BOVINE ENAMEL (생활치 미백제가 우치 법랑질의 투명도에 미치는 영향)

  • Kim, Dong-Jun;Kim, Young-Ju;Kim, Hak-Geun;Park, Eun-Jong;Seo, Eun-Ju;Hwang, Yun-Chan;Oh, Won-Mann;Hwang, In-Nam
    • Restorative Dentistry and Endodontics
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    • v.30 no.3
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    • pp.178-183
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    • 2005
  • This study was done to evaluate whether vital bleaching agents could influence on the translucency of the bovine enamel. The anterior bovine teeth that were extracted one day before and without any gross discoloration were obtained and then were preserved in physiologic saline. 6 mm cylindrical tooth specimens were fabricated with diamond puncher perpendicularly on labial surface of bovine tooth. After embedded in transparent acrylic resin with labial surface being exposed, they were cut to a thickness of 1.2 mm with low speed diamond saw (Isomat, Buehler Co., Lake Bluff, IL, USA). They were smoothly ground to 1 mm thickness of enamel with sandpaper. 24 specimens were randomly divided into 3 groups and control group respectively. Opalescence (10% carbamide peroxide, Ultradent, South Jordan, USA), Rembrandt (10% carbamide peroxide, DenMat, USA) and Opalescence F (15% carbamide peroxide with fluoride, Ultradent, USA) were applied on labial sides of the bovine enamel for 7 days (bleaching agents were reapplied every 24 hours) and the opposite surface was contacted to cotton that soaked in distilled water. The control group was soaked in distilled water. Three stimulus value X, Y and Z were evaluated with colorimeter (Color ' Color Differencerneter, Model TC-6FX, Tokyo Denshoku Co., Japan) on the labial surface of all specimen three times on white and black background plate before the bleaching agents were applied and on 3rd, 5th and 7th day after applied. The degree of translucency was normally assessed by measuring the inverse property, opacity (contrast ratio). 10% Opalescence, 15% Opalescence-F, and control group showed no significant variation in the translucency of bovine enamel, However Rembrandt decreased the translucency of it (p < 0.01).

Machine Transplanting Cultivation with Infant Seedling in Rice Plant II. Effects of Raising Temperature, Duration and Nutritional Residue in Endosperm on Seedling Growth after Transplanting. (벼 어린모(유묘) 기계이앙 재배연구 II. 육묘온도, 육묘일수 및 배유양분잔존량이 이앙후 조기생육에 미치는 영향)

  • Yang, Won-Ha;Yun, Yong-Dae;Song, Moon-Tae;Lee, Moon-Hee;Lim, Moo-Sang;Park, Rae-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.4
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    • pp.434-439
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    • 1989
  • This study was carried out to investigate the minimum days for raising infant rice seedling with different temperature (day/night $^{\circ}C$ : 20/12, 25/18, 30/20) and to compare with seedling growth and rooting ability at 10 days after transplanting with endosperm-intacted and removed seedlings. The minimum days for raising infant rice seedling was shown differently with different temperatures, thus there were turned out by mat formation to be 10 days at 20/12$^{\circ}C$ (day/night), 8 days at 25/18$^{\circ}C$ and 6 days at 30/20$^{\circ}C$, respectively. Seedling height, leaf number and dry weight of top part at the minimum days for raising infant rice seedling were 8-12cm, 1.5-1.7 leaves and 6.9-7. 5cm per seedling, respectively. The seedling growth at 10 days after transplanting was better at high temperatures (25/18$^{\circ}C$) than low temperature (20/12$^{\circ}C$) at transplanting, and the growth of infant rice seedling with endosperm -intacted was better than that with endosperm-removed. This tedency was shown significantly in transplanting at 20/12$^{\circ}C$ (day/night) of 4 days seedling.

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