• 제목/요약/키워드: R-MAT

검색결과 140건 처리시간 0.023초

Identification of Amino Acid Residues in the Carboxyl Terminus Required for Malonate-Responsive Transcriptional Regulation of MatR in Rhizobium leguminosarum bv. trifolii

  • Lee, Hwan-Young;Kim, Yu-Sam
    • BMB Reports
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    • 제34권4호
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    • pp.305-309
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    • 2001
  • MatR in Rhizobium trifolii is a malonate-responsive transcription factor that regulates the expression of genes, matABC, enabling decarboxylation of malonyl-CoA into acetyl-CoA, synthesis of malonyl-CoA from malonate and CoA, and malonate transport. According to an analysis of the amino acid sequence homology, MatR belongs to the GntR family The proteins of this family have two-domain folds, the N-terminal helix-turn-helix DNA-binding domain and the C-terminal ligand-binding domain. In order to End the malonate binding site and amino acid residues that interact with RNA polymerase, a site-directed mutagenesis was performed. Analysis of the mutant MatR suggests that Arg-160 might be involved in malonate binding, whereas Arg-102 and Arg-174 are critical for the repression activity by interacting with RNA polymerase.

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고기능성 침낭형 온수매트 제작 (The Production of High Functional Hot Mat with Sleeping Type)

  • 이상헌;강정욱;원우연
    • 한국전기전자재료학회논문지
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    • 제28권6호
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    • pp.400-402
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    • 2015
  • The typical winter heating unit heating mat. The product is to be found in the development of new water heating mat today. The product that circulates the water through the hose inside the mat with your existing heating mat electric heated mat is different from boiling water. However, no clear standards for noise and safety, consumer choice is giving confusing information about the product is low. We were to develop a high heat retention and stability than conventional sleeping mats to produce creative than traditional hot mats, heated mats general comparison with experimental results is a more efficient heat retention mat is produced.

5-Aza-2'-deoxycytidine Induces Hepatoma Cell Apoptosis via Enhancing Methionine Adenosyltransferase 1A Expression and Inducing S-Adenosylmethionine Production

  • Liu, Wei-Jun;Ren, Jian-Guo;Li, Ting;Yu, Guo-Zheng;Zhang, Jin;Li, Chang-Sheng;Liu, Zhi-Su;Liu, Quan-Yan
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권11호
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    • pp.6433-6438
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    • 2013
  • In hepatocellular cancer (HCC), lack of response to chemotherapy and radiation treatment can be caused by a loss of epigenetic modifications of cancer cells. Methionine adenosyltransferase 1A is inactivated in HCC and may be stimulated by an epigenetic change involving promoter hypermethylation. Therefore, drugs releasing epigenetic repression have been proposed to reverse this process. We studied the effect of the demethylating reagent 5-aza-2'-deoxycitidine (5-Aza-CdR) on MAT1A gene expression, DNA methylation and S-adenosylmethionine (SAMe) production in the HCC cell line Huh7. We found that MAT1A mRNA and protein expression were activated in Huh7 cells with the treatment of 5-Aza-CdR; the status of promoter hypermethylation was reversed. At the same time, MAT2A mRNA and protein expression was significantly reduced in Huh7 cells treated with 5-Aza-CdR, while SAMe production was significantly induced. However, 5-Aza-CdR showed no effects on MAT2A methylation. Furthermore, 5-Aza-CdR inhibited the growth of Huh7 cells and induced apoptosis and through down-regulation of Bcl-2, up-regulation of Bax and caspase-3. Our observations suggest that 5-Aza-CdR exerts its anti-tumor effects in Huh7 cells through an epigenetic change involving increased expression of the methionine adenosyltransferase 1A gene and induction of S-adenosylmethionine production.

SWAT모형을 이용한 유량 및 유사 예측 정확성 평가 및 최적관리 기법 효과 분석 (Evaluation of SWAT Flow and Sediment Estimation and Effects of Soil Erosion Best Management Practices)

  • 이지민;류지철;강현우;강형식;금동혁;장춘화;최중대;임경재
    • 한국농공학회논문집
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    • 제54권1호
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    • pp.99-108
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    • 2012
  • Soil erosion and sediment from agricultural farmland has caused various negative impacts on environment in recent years. The effect of rice straw mat on soil erosion has been investigated by many researchers these days. In this study, the SWAT model was applied to Hongcheon watershed to evaluate SWAT flow and sediment, and the effect of rice straw mat on sediment yield at watershed outlet was evaluated. The Nash-Sutcliffe model efficiency (NSE) and coefficient of determination ($R^2$) values for flow simulation (calibration period) were 0.66 and 0.67, and the NSE values for sediment was 0.90. The calibrated parameters were used to analyze the reduction of sediment yield in the farmland with rice straw mat. Average daily sediment yield without rice straw mat was 49.8 ton/day and sediment yield with rice straw mat was 25.5 ton/day, and the reduction rate was 38.7 %. Also, average daily sediment yield with/without rice straw mat were 97.5 ton/day and 190.7 ton/day during the rainy season (Jun. 2008 - Aug. 2009), with the reduction rate 46.3 %.

실리콘을 첨가한 주석 산화물 박막의 전기 화학적 특성 (Electrochemical Characteristics of Silicon-Doped Tin Oxide Thin Films)

  • 이상헌;박건태;손영국
    • 한국재료학회지
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    • 제12권4호
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    • pp.240-247
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    • 2002
  • Tin oxide thin films doped with silicon as anodes for lithium secondary battery were fabricated by R.F. magnetron sputtering technique. The electrochemical results showed that the irreversible capacity was reduced during the first discharge/charge cycle, because the audition of silicon decreased the oxidic state of Tin. Capacity was increased with the increase of substrate temperature, however decreased with the increase of RTA temperatures. The reversible capacity of thin films fabricated under the substrate temperature of $300^{\circ}C$ and the Ar:$O_2$ratio of 7:3 was 700mA/g.