Two soybean cultivars, ‘Lee’ and ‘Peking’, were used to evaluate the competition and interaction of rhizobium strains PRC205 (R. fredii, fast-grower) and USDA110 (B. japonicum, slow-grower). Strains were inoculated separately on the root parts of a split-root growth system. Both root sides were inoculated simultaneously with four combinations of strain treatment to evaluate the competition of strains. And to evaluate the interaction of strains one side of split-root system was inoculated a week prior to the other side. Nodule mass and dry weight of the plants were measured 3 weeks after treatments. PRC205 showed no effective nodulation and no competing ability with USDA110 on Lee cultivar, however, contrary results on Peking cultivar. Top dry weight of Lee inoculated with PRC205 was much lower than that of any other inoculation treatments, however, in Peking that with PRC205 was higher than that with USDA110. There were no differences in root dry weight among the inoculation treatments. USDA110 used as primary inoculant suppressed nodule mass of opposite side, secondary inoculant, severely in both cultivars. PRC205 showed same tendency as USDA110 in Peking, but revealed little suppression effects on USDA110 used as secondary inoculant in Lee. USDA110 used as primary inoculant in Lee and PRC205 in Peking showed much more dry weight of soybean plants than that of other treatments.
Objectives: To explore the expression of astrocyte elevated gene-1 (AEG-1) in cervical cancer and analyze its correlation with microvascular density (MVD), nuclear factor kappaB (NF-kB p65) and vascular endothelial growth factor (VEGF). Materials and Methods: Immunohistochemical MaxVision method was adopted to detect the expression level of AEG-1, NF-kB p65 and VEGF in 45 samples of invading cervical cancer and 12 samples of cervicitis from The First Affiliated Hospital of Wenzhou Medical University. Tumor microvascular endothelial marker CD34 combined with Weidner was used to determine the MVD in cervical cancer tissue. The positive expression and staining conditions of AEG-1, NF-kB p65 and VEGF in cervical cancer tissues were observed under a light microscope. Correlations between expression of AEG-1 protein and those of NF-Kb p65 and VEGF, as well as MVD, were analyzed using Pearson correlation. Results: The expression levels of AEG-1 were $0.186{\pm}0.043$ in cervical cancer and $0.051{\pm}0.002$ in chronic cervicitis (p<0.01). Moreover, expression of AEG-1 was related to vascular invasion and lymphatic metastasis of cervical cancer (p<0.01), but not with age of the patients, differentiation degree, tumour size, pathological type and parametrial infiltration (p>0.05). Pearson correlation analysis showed that the expression of AEG-1 was linked with NF-kB p65 (r=0.501, p=0.000), VEGF (r=0.718, p=0.000) as well as MVD in cervical cancer tissue (r=0.815, p=0.000). Conclusions: AEG-1 is highly expressed in cervical cancer and promotes angiogenesis, which might be related to the fact that AEG-1 activating the signal pathway of NF-kB could up-regulate the level of VEGF expression.
Objectives: The purpose of the study is to investigate the correlation and influencing factors of oral health awareness, oral health behaviors, self-esteem and OHIP-14. Methods: A self-reported questionnaire was filled out by 313 childcare teachers in Jeonnam from June 4 to 14, 2013. The questionnaire consisted of 3 questions of general characteristics, 4 questions of occupation, 1 question of oral health education experience, and 1 question of oral health education participation. The instrument for awareness and behavior of oral health were modified and consisted of 10 questions of awareness and 10 questions of behavior by Likert 5 scale. Cronbach's alpha was 0.718 in awareness and 0.812 in behavior. Instrument for self-esteem was modified from Rosenberg. Self-esteem questionnaire consisted fo 5 questions of positive answers and 5 questions of negative answers by Likert 5 scale. Cronbach's alpha in self esteem was 0.846 in the study. Oral Health Impact Profile-14(OHIP-14) was adapted from Slade by Likert 5 scale and consisted fo 14 questions. Cronbach's alpha was 0.934 in the study. Data were analyzed by chi square test, t-test, one way ANOVA, Scheffe multiple range test, Pearason's correlation test, and stepwise multiple regression test. Results: There were positive correlations between oral health awareness, oral health behavior(r=0.502), and self-esteem(r=0.332), but negative correlations with OHIP-14. Oral health behavior showed positive correlations with self-esteem(r=0.230). The factors on oral health awareness were high oral health behavior and self esteem, low OHIP-14, and active participation in education. Self-esteem was closely related to high with high oral health awareness. low OHIP-14, low job satisfaction. Conclusions: Childcare teachers play the very important roles in the development of oral health education program for children and continuous education.
Most cattle breeds have a coat color pattern that is characteristic for the breed. Korean cattle(Hanwoo) has a coat color ranging from yellowish brown to dark brown including a red coat color. Variation in the Hanwoo coat color is likely to be the effects of modified genes segregating within the Hanwoo breed. MC1R encoded by the Extension(E) locus was almost fixed with recessive red e allele in the Hanwoo, but other gene(s) might be affecting the variation of the Hanwoo coat color into yellowish to red brown. We have analyzed a segregation of coat color in the F2 families generated from two Hanwoo bulls(yellowish brown) mated to six F1 dams(black) derived from Hanwoo and Holstein crosses. Segregation of coat color in the offspring found a ratio of 1(yellowish brown) : 1(black) and this ratio indicates that a single gene may play a major role for the Hanwoo coat color. We further investigated SNPs in MC1R, ASIP and TYRP1 loci to determine genetic cause of the Hanwoo coat color. Several polymorphisms within ASIP intron 2 and TYRP1 exons were found but not conserved within the Hanwoo population. However, the segregation of the MC1R e allele was completely associated with the Hanwoo coat color. Based on this information, it is clear that the MC1R e allele is mainly responsible for the yellowish red Hanwoo coat color. Further study is warrant to identify possible genetic interaction between MC1R e allele and other coat color related gene(s) for the variation of Hanwoo coat color from yellowish brown to dark brown. (Key words : Hanwoo, Coat color, SNP, MC1R, ASIP, TYRP1)
Korean native chickens are a very valuable chicken population in Korea and their prices are higher than that of commercial broilers. In order to discriminate two commercial Korean native chicken populations (CCP1 and CCP2), single nucleotide polymorphisms (SNPs) from mitochondrial (mt) DNA D-loop sequences and LEI0258 marker polymorphisms in the major histocompatibility complex (MHC) region were investigated. A total of 718 birds from nine populations were sampled and 432 mtDNA sequences were obtained. Of these, two commercial Korean native chicken populations (363 birds) were used for investigation of their genetic relationship and breed differentiation. The sequence data classified the chickens into 20 clades, with the largest number of birds represented in clade 1. Analysis of the clade distribution indicated the genetic diversity and relation among the populations. Based on the mtDNA sequence analysis, three selected SNPs from mtDNA polymorphisms were used for the breed identification. The combination of identification probability (Pi) between CCP1 and CCP2 using SNPs from mtDNA and LEI0258 marker polymorphisms was 86.9% and 86.1%, respectively, indicating the utility of these markers for breed identification. The results will be applicable in designing breeding and conservation strategies for the Korean native chicken populations and also used for the development of breed identification markers.
Purpose: The purpose of this study was to identify the relationship between fatigue, and self-efficacy in patients with lung cancer. Method: The data was collected from January 21th to April 8th 2001. The study subjects were recruited from K hospital in Pusan, Korea. Their fatigue was measured using the 22-item Revised Piper Fatigue Scale developed by Piper et al. (1998) and translated by the investigators, and self-efficacy was measured using the 10-item General Self-efficacy Questionnaire developed by Lee, Schwarzer & Jerusalem. The data were analyzed by SPSS 10.0 program using frequency, percentage, mean and standard deviation, t-test, ANOVA, Post-hoc test (Scheffe's test), and Pearson Correlation Coefficients. Result: 1) The mean fatigue score was $118.48{\pm}33.02$ (range: 22-220). The scores for sub-dimension were $35.40{\pm}10.06$ in behavior/severity, $25.98{\pm}9.06$ in affective score, $27.88{\pm}10.06$ in sensory score, and $29.22{\pm}8.27$ in cognitive/mood. The mean self-efficacy score was $28.80{\pm}5.85$. 2). There were significant differences in the fatigue of patients with lung cancer on income per month (F=4.651, p= .014), 'present pain' (F=2.601, p= .012), 'change of weight' (F=5.911, p= .005), by general characteristics. 3) There were significant differences in the self-efficacy of patients with lung cancer on 'religion' (F=3.732, p= .031), 'employment status' (F=5.525, p= .003), 'past therapy' (F=2.869, p= .034), by general characteristics 4) There was a significant negative correlation between fatigue and self-efficacy (r=- .528, p= .000). Conclusion: Patients with lung cancer experience fatigue. Increased fatigue is associated with decreased self-efficacy. Nurses must provide patients with nursing care for the less occurrence of fatigue and interventions to manage self-efficacy for them.
The purpose of this study was to identity degree on self-care agency & self efficacy of hemodialisis patients. The subjects for the study consisted of 260 hemodialisis patients and the data were collected from April 1st. 2002 to April 30. 2002. The instruments used for this study were the Self-carer As Inventory Scale(Genden & Taylor. 1998) and Concrete Self Efficacy Scale(Joo-Hyen Kim. 1995). The Self-carer As Inventory translated by So Hyang-Sook and was modified by auther of this study. The data were analysed by using SPSS/WIN program and included number. percentange. t-test, ANOV A and Pearson Correlation Coefficient. The results of the study are summarized as follows. 1. As the average score Self-care agency indicated $132.21\pm19.11$ points and Self efficacy showed $597.33\pm130.95$points. it can be said that Self-care agency and Self efficacy were high. 2. In the relationship between general and hemodialisis cure characteristics and Self care agency were significant difference in age(F=3.065. p=0.018). married(F=3.160. p= 0.029). religion(F=4.128. p=0.003). hemodialisis cure duration(F=2.615. p=0.049). 3. In the relationship between general and hemodialisis cure characteristics and Self efficacy were significant difference in age (F=2.992. p=0.019). married(F=4.427. p=0.013), job(before attack-F=1.926. p=0.044. after attack-F=1.784. p=0.048). incomes (F=2.235. p=0.041), hemodialisis cure frequency (F=1.718. p=0.042). diet practice (F=2.248. p=0.025). 4. Self efficacy was significantly related to Self-care agency(r=0.474. p<(0.001). That is the higher the self efficacy. the higher the level of Self-care agency. The result in the above showed that more concern and endeavors need to improve hope. medical support. self efficacy, self-care agency of hemodialisis patients.
The purpose of this study was to investigate physical self concept between high school boys and girls and to find various factors affecting them. For the Survey, 331 respondents for boys and 347 for girls were randomly selected from one coeducational high school in Seoul, from May 16 to May 19, 1990. The instrument used in this questionaire survey method was a part of Won Shik Jung's Physical Self Concept Scale for measuring Self Concept, it's reliability was' Cronbach a = 0.718. For the analysis. descriptive statistics were used by calculating frequencies, percentages and mean scores. $X^2-test$, t-test, and analysis of variance were employed to test the differences and statistical significance. The relationship between physical self concept and area variables was analysed by use of Pearson's correlation coiefficients. For the examine of various factors affecting physical self concept, multiple stepwise regression method was employed. The results of this study are summarized as follows: 1. There was no significant difference in physical self concept between male and female students. 2. The scores of physical self concept among the students were negatively correlated with their academic year, even though statistical signification was not found. 3. Physical self concept was classified into two areas; health area and appearance area. There was no significant difference in health area between boys and girls. But, the difference of health area was significant in accordance with the academic years. That, the scores of the health area are dropped as academic year going up. 4. Significant relationship was revealed among health area, appearance area, and physical self concept. The correlation between health area and physical self concept was the highest relationship (r = 0.71) The main factors affecting the physical self concept were (1) father's educational background (2) year (3) number of brother (4) religion (5) father's age. 5. As academic year going up, the rate of counseling with parents are dropped. In conclusion, the result of the study indicate that, a person managed the school health and parents can help students to promote their physical, mental, and emotional health by means of health education and counseling.
Objective: The incidence rate of elbow ulnar collateral ligament injuries is dependent on the throwing speed or pitching type, especially in adolescent baseball players. However, mixed results have been reported due to a lack of controlled biomechanical analysis. Thus, the purpose of this study was to investigate the biomechanical analysis of the elbow in relation to throwing speed and pitching type. Method: Four overhead type high-school baseball players were recruited for this study. The participants were asked to throw balls with different types of pitch and speed. While the throwing speeds were measured, each pitching moment of the elbow was recorded. Descriptive statistics, frequency analysis, mean comparison analysis, and Pearson's correlation analysis were performed in order to examine differences in peak varus and valgus moment during pitching motion in the elbow in all throwing speed and pitching types. Results: There was no significant difference in physical characteristics, throwing speed, and momentum variability among all players. The mean varus moments were 44.38±1.55 Nm, 48.83±1.66 Nm, and 48.94±0.95 Nm, and the moment gaps between varus and valgus were 7.36±3.25 Nm, 7.44±2.02 Nm, and 7.36±2.62 Nm in fastball, curveball, and slider ball, respectively. The varus moment was higher in the curved and slider balls than in the fastballs, and there was no significant differences between the varus moments regarding the pitching type. However, the increase in valgus moment and decrease in moment gap according to throwing speed was significantly increased in the slider ball (r=0.718 and -0.591, respectively). Conclusion: The possibility of elbow injury caused by the valgus moment or moment gapincreases more rapidly in slider balls as the speed increases. Based on our results, appropriate pitching guidelines should be suggested to prevent ulnarligament injuries, especially in adolescent baseball players.
Purpose: To identify perceived importance and performance frequency of nursing interventions with nursing activities of 5 nursing interventions from the physiological domains of NIC used in the home health care nursing. Method: Five nursing interventions in the physiological domain of NIC were selected based on the previous finding. Data were collected from 85 nurses working in 54 home health care centers between August and October, 2004 using mailing survey(return rates : 41.5%). The questionnaire consisted of 96 nursing activities in 5 interventions with definitions, asking perceived importance and performance frequency of them. Result: Skin surveillance was perceived as the most important($3.52{\pm}0.36$) intervention and also performed most frequently($4.43{\pm}0.45$). All the nursing activities in skin surveillance appeared to be used frequently, which was rated over 4 out of 5 point Likert, while only 4 nursing activities in exercise therapy: joint mobility did. Correlations between perceived importance and performance frequency of 5 interventions were all significant (r=0.591-0.718, p=0.000). Conclusion: Identifying the use of particular interventions and nursing activities will help nurses simplify documentations and to deliver better care to the patients in home health care nursing.
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