• Parasites, Hosts and Diseases
• /
• v.49 no.4
• /
• pp.445-448
• /
• 2011

A survey was performed to find out the intermediate hosts of Gnathostoma nipponicum in Jeju-do (Province), the Republic of Korea. In August 2009 and 2010, a total of 82 tadpoles, 23 black-spotted pond frogs (Rana nigromaculata), 7 tiger keelback snakes (Rhabdophis tigrinus tigrinus), 6 red-tongue viper snakes (Agkistrodon ussuriensis), and 2 cat snakes (Elaphe dione) were collected in Jeju-do and examined by the pepsin-HCl digestion method. Total 5 gnathostome larvae were detected in 3 (50%) of 6 A. ussuriensis, 70 larvae in 3 of 7 (42.9%) R. tigrinus tigrinus, and 2 larvae in 2 of 82 (8.7%) frogs. No gnathostome larvae were detected in tadpoles and cat snakes. The larvae detected were a single species, and $2.17{\times}0.22mm$ in average size. They had characteristic head bulbs, muscular esophagus, and 4 cervical sacs. Three rows of hooklets were arranged in the head bulbs, and the number of hooklets in each row was 29, 33, and 36 posteriorly. All these characters were consistent with the advanced third-stage larvae of G. nipponicum. It has been first confirmed in Jeju-do that R. nigromaculata, A. ussuriensis, and R. tigrinus tigrinus play a role for intermediate and/or paratenic hosts for G. nipponicum.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.3
• /
• pp.448-458
• /
• 2020

We investigated the therapeutic effects of microRNA-139-5p in relation to osteoporosis of bone marrow-derived mesenchymal stem cell (BMSCs) and its underlying mechanisms. In this study we used a dexamethasone-induced in vivo model of osteoporosis and BMSCs were used for the in vitro model. Real-time quantitative polymerase chain reaction (RT-PCR) and gene chip were used to analyze the expression of microRNA-139-5p. In an osteoporosis rat model, the expression of microRNA-139-5p was increased, compared with normal group. Down-regulation of microRNA-139-5p promotes cell proliferation and osteogenic differentiation in BMSCs. Especially, up-regulation of microRNA-139-5p reduced cell proliferation and osteogenic differentiation in BMSCs. Overexpression of miR-139-5p induced Wnt/β-catenin and down-regulated NOTCH1 signaling in BMSCs. Down-regulation of miR-139-5p suppressed Wnt/β-catenin and induced NOTCH1 signaling in BMSCs. The inhibition of NOTCH1 reduced the effects of anti-miR-139-5p on cell proliferation and osteogenic differentiation in BMSCs. Activation of Wnt/β-catenin also inhibited the effects of anti-miR-139-5p on cell proliferation and osteogenic differentiation in BMSCs. Taken together, our results suggested that the inhibition of microRNA-139-5p promotes osteogenic differentiation of BMSCs via targeting Wnt/β-catenin signaling pathway by NOTCH1.

• BMB Reports
• /
• v.40 no.3
• /
• pp.419-425
• /
• 2007

In vitro directed evolution through DNA shuffling is a powerful molecular tool for creation of new biological phenotypes. E. coli $\beta$-galactosidase and $\beta$-glucuronidase are widely used, and their biological function, catalytic mechanism, and molecular structures are well characterized. We applied an in vitro directed evolution strategy through DNA shuffling and obtained five mutants named YG6764, YG6768, YG6769, YG6770 and YG6771 after two rounds of DNA shuffling and screening, which exhibited more $\beta$-glucuronidase activity than wild-type $\beta$-galactosidase. These variants had mutations at fourteen nucleic acid sites, resulting in changes in ten amino acids: S193N, T266A, Q267R, V411A, D448G, G466A, L527I, M543I, Q626R and Q951R. We expressed and purified those mutant proteins. Compared to the wild-type protein, five mutant proteins exhibited high $\beta$-glucuronidase activity. The comparison of molecular models of the mutated and wildtype enzymes revealed the relationship between protein function and structural modification.

• Journal of the Korea Institute of Military Science and Technology
• /
• v.24 no.4
• /
• pp.435-448
• /
• 2021

This study describes the depth and straight motion control performance depending on control surface combinations of a supercavitating underwater vehicle. When an underwater vehicle experiences supercavitation, friction resistance can be minimized, thus achieving the effect of super-high-speed driving. Six degrees of freedom modeling of the underwater vehicle are performed and the guidance and control loops are designed with not only a cavitator and an elevator, but also a rudder and a differential elevator to improve the stability of the roll and yaw axis. The control performance based on the combination of control surfaces is analyzed by the root-mean-square error for keeping depth and straight motion.

• Korean Journal of Applied Biomechanics
• /
• v.29 no.3
• /
• pp.173-183
• /
• 2019

Objective: The aim of this study was to investigate the correlation coefficients between anthropometric parameters of the foot and kinetic variables during running. Method: This study was conducted on 21 healthy young adults (age: $24.8{\pm}2.1yes$, height: $177.2{\pm}5.8cm$, body mass: $73.3{\pm}7.3kg$, foot length: $256.5{\pm}12.3mm$) with normal foot type and heel strike running. To measure the anthropometric parameters, radiographs were taken on the frontal and sagittal planes, and determined the length and width of each segment and the navicular height. Barefoot running was performed at a preferred velocity ($3.0{\pm}0.2m/s$) and a fixed velocity (4.0 m/s) on treadmill (Bertec, USA) in order to measure the kinetic variables. The vertical impact peak force, the vertical active peak force, the braking peak force, the propulsion peak force, the vertical force at mid-stance (vertical ground reaction when the foot is fully landed in mid-stance or at the point where the weight was uniformly distributed on the foot) and the impact loading rate were calculated. Pearson's correlation coefficient was used to investigate the relationship between anthropometric variables and kinetical variables. The significance level was set to ${\alpha}=.05$. Results: At the preferred velocity running, the runner with longer forefoot had lower active force (r=-.448, p=.041) than the runner with short forefoot. At the fixed velocity, as the navicular height increases, the vertical force at full landing moment increases (r= .671, p= .001) and as the rearfoot length increases, the impact loading rate decreases (r=- .469, p= .032). Conclusion: There was a statistically significant difference in the length of fore-foot and rearfoot, and navicular height. Therefore it was conclude that anthropometric properties need to be considered in the foot study. It was expected that the relationship between anthropometric parameters and kinetical variables of foot during running can be used as scientific criteria and data in various fields including performance, injury and equipment development.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.29 no.6
• /
• pp.441-448
• /
• 2018

Recently, measurements on clutter loss due to buildings in urban/suburban areas at 3, 6, 10, 18, and 24 GHz have been performed by the Radio Research Agency with the purpose of predicting the clutter loss close to actual urban/suburban propagation for 5G mobile communication. In this work, we have compared the urban clutter loss to suburban clutter loss for a transmit antenna height of 85 m. Furthermore, we have estimated the error between the predicted loss as per ITU-R P.2108 and the measured clutter loss. Our results indicate that for higher frequencies, the measured clutter loss in urban/suburban areas is higher and so lower than the predicted clutter loss. In conclusion, it is necessary to improve the prediction model for clutter loss by taking into account the measured clutter loss in urban/suburban areas in the prediction model.

• Tuberculosis and Respiratory Diseases
• /
• v.55 no.5
• /
• pp.440-448
• /
• 2003

Background : Development of rapid drug susceptibility testing provides the opportunity for rapid identification of individuals with drug resistant tubercle bacilli, allowing selection of appropriate therapeutic regimens. Methods : A total of 502 drug resistant isolates were subjected to reverse blot hybridization assay to detect mutations within genes (rpoB, katG, inhA, and ahpC) associated with rifampicin (RMP) and isoniazid (INH) resistance. Results : Among the 264 RMP resistant strains ($RMP^R$) tested, the most prevalent mutation was the Ser531Leu seen in 121 strains (46%). The second common mutation occurred in 84 strains (32%) at codon 526. And 27 strains (10%) showed the mutation at codon 516. Among all 469 INH resistant strains ($INH^R$), the katG mutation was responsible for INH. The inhA mutation was present in 88 strains (19%). In 11 isolates (2%), coexisting of the katG and inhA mutations were identified. Reverse hybridization assay successfully detected over 80% of $INH^R$ and over 92% of $RMP^R$ among Korean isolates. CONCLUSION: Reverse hybridization was useful for rapid detection of $INH^R$ and $RMP^R$.

• Journal of Korean Academy of Nursing
• /
• v.36 no.3
• /
• pp.439-448
• /
• 2006

Purpose: The purpose of this study was to show a relationship between health perception and health promoting behaviors in chronic low back pain patients. Method: The subjects for this study were 213 persons who the visited hospital with low back pain-related problems. Results: The higher the level of the health perception in chronic back pain patients was the higher the rate of the practice of health promoting behaviors (r=0.393, p<.001). The health perception T score was $50.00{\pm}10.00$. As for health promoting behaviors, the T score was $49.99{\pm}10.00$. The subscale of the highest mean score was interpersonal support $(2.96{\pm}0.64)$ and the subscale of the lowest mean score was exercise $(2.13{\pm}0.99)$. Conclusion: This study showed that chronic low back pain patients had a lower level of perception of their health, and their practice to improve their health was not enough. Therefore, nurses should educate and encourage chronic low back pain patients in proper exercises and correct posture to strengthen and maintain lumbar extension muscle power.

• Mass Spectrometry Letters
• /
• v.6 no.4
• /
• pp.112-115
• /
• 2015

In this article, we examine the influences of Ne and He buffer gases under confined Ar⁺ ion cloud in a homemade Paul ion trap in various pressures and confinement times. The trap is of small size (r₀ = 1 cm) operating in a radio frequency (rf) voltage only mode, and has limited accuracy of 13 V. The electron impact and ionization process take place inside the trap and a Faraday cup has been used for the detection. Although the experimental results show that the Ar⁺ ion FWHM with Ne buffer gas is wider than the He buffer gas at the same pressure (1×10^-1 mbar) and confinement time is about 1000 μs, nevertheless, a faster cooling was found with He buffer gas with 500 μs. ultimetly, the obtanied results performed an average cloud tempertures reduced from 1777 K to 448.3 K for Ne (1000 μs) and from 1787.9 K to 469.4 K for He (500 μs)

• Ocean Systems Engineering
• /
• v.9 no.4
• /
• pp.423-448
• /
• 2019

Very Large Floating Structures (VLFS) are one among the solution to pursue an environmentally friendly and sustainable technology in birthing land from the sea. VLFS are extra-large in size and mostly extra-long in span. VLFS may be classified into two broad categories, namely the pontoon type and semi-submersible type. The pontoon-type VLFS is a flat box structure floating on the sea surface and suitable in regions with lower sea state. The semi-submersible VLFS has a deck raised above the sea level and supported by columns which are connected to submerged pontoons and are subjected to less wave forces. These structures are very flexible compared to other kinds of offshore structures, and its elastic deformations are more important than their rigid body motions. This paper presents hydroelastic analysis carried out on an innovative VLFS called truss pontoon Mobile Offshore Base (MOB) platform concept proposed by Srinivasan and Sundaravadivelu (2013). The truss pontoon MOB is modelled and hydroelastic analysis is carried out using HYDRAN-XR^* for regular 0° waves heading angle. Results are presented for variation of added mass and damping coefficients, diffraction and wave excitation forces, RAOs for translational, rotation and deformational modes and vertical displacement at salient sections with respect to wave periods.