• Asian Pacific Journal of Cancer Prevention
• /
• 제17권11호
• /
• pp.4959-4963
• /
• 2016

Objective:Cervical cancer is one of the major causes of mortality of Uyghur women in Xinjiang, China. Although increased expression of orexin receptor (OxR), known to be strongly expressed in human placenta, has a proven relation to some cancers, there have been few studies of cervical cancer. Thus, we explored this question by evaluating the expression of orexin receptor as a biomarker for screening early stage of cervical cancer in Uyghur women with highest occurrence rate of cervical cancer in China. Study Design: We used polymerase chain reaction (PCR) and immunohistochemical staining to determine the expression of both Ox1R and Ox2R in cervical cancer and cervicitis biopsies collected from Uyghur women infected with human papilloma virus (HPV)16. The expression rate was compared between cervical cancers of low, intermediate and high differentiation and cervicitis. Results:Although there was no significant difference in the expression rate of Ox1R between groups, Ox2R was significantly overexpressed in cervical cancer patients when compared to the cervicitis group. Ox1R was negative in normal human placenta while Ox2R was positive. Conclusions: While expression of Ox1R had no correlation with invasion or metastatic potential, Ox2R demonstrated elevation in cervical cancer with heterogeneity in groups with different metastatic potential, in the human placenta as well, implying that it might serve as an indicator of invasive capacity along with other indices.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권12호
• /
• pp.4937-4944
• /
• 2015

MicroRNAs (miRNAs) are emerging as critical regulators in carcinogenesis and tumor progression. Recently, miR-99a has been reported as a tumor suppressor gene in various human cancers, but its functions in the context of anaplastic thyroid cancer (ATC) remain unknown. In this study, we reported that miR-99a was commonly downregulated in ATC tissue specimens and cell lines with important functional consequences. Overexpression of miR-99a not only dramatically reduced ATC cell viability by inducing cell apoptosis and accumulation of cells at G1 phase, but also inhibited tumorigenicity in vivo. We then screened and identified a novel miR-99a target, mammalian target of rapamycin (mTOR), and it was further confirmed by luciferase assay. Up-regulation of miR-99a would markedly reduce the expression of mTOR and its downstream phosphorylated proteins (p-4E-BP1 and p-S6K1). Similar to restoring miR-99a expression, mTOR down-regulation suppressed cell viability and increased cell apoptosis, whereas restoration of mTOR expression significantly reversed the miR-99a antitumor activity and the inhibition of mTOR/p-4E-BP1/p-S6K1 signal pathway profile. In clinical specimens and cell lines, mTOR was commonly overexpressed and its protein levels were statistically inversely correlated with miR-99a expression. Taken together, our results demonstrated for the first time that miR-99a functions as a tumor suppressor and plays an important role in inhibiting the tumorigenesis through targeting the mTOR/p-4E-BP1/p-S6K1 pathway in ATC cells. Given these, miR-99a may serve as a novel prognostic/diagnostic and therapeutic target for treating ATC.

• Journal of Korea Trade
• /
• 제23권5호
• /
• pp.118-128
• /
• 2019

Purpose - This paper examines the international R&D contest in which the extent of intellectual property right (IPR) affects both the size of prize for the winning firm and the extent of positive spillover through cost of firms. Recognizing the possibility of incomplete protection of IPR, the present paper analyzes the effect of changes in the extent of IPR on payoffs to firms and social welfare. Design/methodology - This paper examines coordination of IPRs by countries in economic integration. The paper then develops a general model of international R&D contest with incomplete protection of IPR. An increase in the extent of IPR augments the share of the prize the winning firm can appropriate, while decreasing the positive cost externality. To derive sharper results, the paper considers the cases of linear and fixed spillovers. Findings - Under plausible assumptions, an increase in the IPR augments the payoff to each firm and the aggregate payoffs as well. The paper also shows that the number of firms participating in the R&D contest can be endogenously determined in the two-stage R&D contest. The higher the extent of cost spillover, and the larger the effective prize, the more firms participate in the international R&D contest. Originality/value - Existing studies assume that firms winning the R&D contest enjoy perfect IPR to the output of their R&D activities. This is a very restrictive assumption in that other firms can copy the new products or processes. By allowing for the incompleteness of the IPR, the present paper develops a more realistic model of R&D contest. The novelty of the present paper is to allow for the possibility that the higher extent of IPR increases the prize and decreases positive cost externality at the same time. The findings of the present paper can serve as a basis for government policy toward R&D activities of firms and protection of IPRs.

• Computers and Concrete
• /
• 제5권4호
• /
• pp.375-388
• /
• 2008

Crack opening governs many transfer properties that play a pivotal role in durability analyses. Instead of trying to combine continuum and discrete models in computational analyses, it would be attractive to derive from the continuum approach an estimate of crack opening, without considering the explicit description of a discontinuous displacement field in the computational model. This is the prime objective of this contribution. The derivation is based on the comparison between two continuous variables: the distribution if the effective non local strain that controls damage and an analytical distribution of the effective non local variable that derives from a strong discontinuity analysis. Close to complete failure, these distributions should be very close to each other. Their comparison provides two quantities: the displacement jump across the crack [U] and the distance between the two profiles. This distance is an error indicator defining how close the damage distribution is from that corresponding to a crack surrounded by a fracture process zone. It may subsequently serve in continuous/discrete models in order to define the threshold below which the continuum approach is close enough to the discrete one in order to switch descriptions. The estimation of the crack opening is illustrated on a one-dimensional example and the error between the profiles issued from discontinuous and FE analyses is found to be of a few percents close to complete failure.

• 한국응용과학기술학회지
• /
• 제9권2호
• /
• pp.107-117
• /
• 1992

The synthesis and solution properties of polyglycerol alkyl ether($R_{12}Gn$) are described. The phase behavior, surface tension, cloud point and HLB value of polyglycerol dodecyl ether in aqueous solution and in mixed solution of surfactant /water /oil have been investigated and compared with values of polyoxyethylene dodecyl ether. The surface tension showed that $R_{12}Gn$ have sufficiently low values of surface tension and cmc to serve as useful polyoxyethylene alkyl ether. The mesophases appearing in the $R_{12}Gn$ systems were more stable in a high temperature range than the mesophases of polyoxyethylene alkyl ether systems. The cloud point and HLB data indicated that addition of one glycerol group was equivalent to the addition of three oxyethylene group units, as far as the hydrophilic property was concerned. The phase diagrams of the polyglycerol alkyl ether /dodecane /water systems showed that the solubilizing and emulsifying powers of $R_{12}Gn$ were greater than those of polyoxyethylene alkyl ether. It is concluded that the polyglycerol chain can be even more useful as hydrophilic group of nonionic surfactants than the polyoxyethylene chain.

• Journal of Microbiology and Biotechnology
• /
• 제34권4호
• /
• pp.911-919
• /
• 2024

Solar UVB irradiation cause skin photoaging by inducing the high expression of matrix metalloproteinase (MMPs) to inhibit the expression of Type1 procollagen synthesis. 1-Kestose, a natural trisaccharide, has been indicated to show a cytoprotective role in UVB radiation-induced-HaCaT cells. However, few studies have confirmed the anti-aging effects. In the present study, we evaluated the anti-photoaging and pathological mechanism of 1-kestose using Human keratinocytes (HaCaT) cells. The results found that 1-kestose pretreatment remarkably reduced UVB-generated reactive oxygen species (ROS) accumulation in HaCaT cells. 1-Kestose suppressed UVB radiation-induced MMPs expressions by blocking MAPK/AP-1 and NF-κB p65 translocation. 1-Kestose pretreatment increased Type 1 procollagen gene expression levels by activating TGF-β/Smad signaling pathway. Taken together, our results demonstrate that 1-kestose may serve as a potent natural trisaccharide for inflammation and photoaging prevention.

• International Journal of Knowledge Content Development & Technology
• /
• 제10권2호
• /
• pp.39-49
• /
• 2020

Accurate and reliable Information improves decision making, reduces costs, and saves time. The ophthalmic Libraries supply quality ophthalmic information to the ophthalmologists to serve the patients in a better way and stimulate the growth of ophthalmic field. This paper aims to study the usage of library collection, services and assistance among the Ophthalmologists - Eye Doctors in India. A survey method is used to obtain the ophthalmologists' usage of library collection, services and assistance. Through convenient sampling method, a structured questionnaire is circulated to the ophthalmologists in India and 633 ophthalmologists are responded. Among the 633 ophthalmologist, 82.15% of the ophthalmologists use the library collection - book. 73.46% of the ophthalmologists use the online data / journals. 89.73% of the ophthalmologists seek assistance to locate books/articles/documents. There exists a significant difference between the ophthalmologists use of Library Service and gender (p value 0.001**). There exists a significant difference between the ophthalmologists use of Library Assistance / help and designation category (p value 0.000**). There exists a significant difference between the ophthalmologists use of Library Assistance / help and working experience (p value 0.017**). There exists a significant difference between Library Services (p value 0.009**), Library Assistance / help (p value 0.000**) and institution type. The study results will help the ophthalmic libraries to serve the user better.

• Smart Structures and Systems
• /
• 제3권3호
• /
• pp.321-340
• /
• 2007

Structural control technologies have attracted great interest from the earthquake engineering community over the last few decades as an effective method of reducing undesired structural responses. Traditional structural control systems employ large quantities of cables to connect structural sensors, actuators, and controllers into one integrated system. To reduce the high-costs associated with labor-intensive installations, wireless communication can serve as an alternative real-time communication link between the nodes of a control system. A prototype wireless structural sensing and control system has been physically implemented and its performance verified in large-scale shake table tests. This paper introduces the design of this prototype system and investigates the feasibility of employing decentralized and partially decentralized control strategies to mitigate the challenge of communication latencies associated with wireless sensor networks. Closed-loop feedback control algorithms are embedded within the wireless sensor prototypes allowing them to serve as controllers in the control system. To validate the embedment of control algorithms, a 3-story half-scale steel structure is employed with magnetorheological (MR) dampers installed on each floor. Both numerical simulation and experimental results show that decentralized control solutions can be very effective in attaining the optimal performance of the wireless control system.

• 한국도서관정보학회지
• /
• 제1권
• /
• pp.1-15
• /
• 1974

The public library and micro-library as institutions of social education have been greatly developed under the influence of the library law enacted 011 Octob~r 28,1963 and the micro-library movement begun in 1961. However, they have still yet many problems to solve in order to accomplish their purpose effectively. As compared with the staff and library materials, buildings are 1norc. than a match for the others. Although many facilities are not complete enough to serve the public, people do not make the best of the facilities in both the public libraries and micro-libraries. The cause of such a result ~vould be taken from a reason that the founders of the libraries could not concentrate their intcrest on the staff and materials, for they were bent on the establishment of external facilities of the libraries. The poorer materials and facilities are, the more effort a2d better quality of the staff are required for their best utilization. As it is the information center of the community, the management of a library should be scientific and reasmable. In addition, librarians have to do their best to serve the public with the spirit of Christianity under the more active suppxt of the government. Finally, institutions of social education in this comn~unityin cluding public libraries and micro-libraries might establish a mutual cooperative organization for more effective library service.

• Genomics & Informatics
• /
• 제15권3호
• /
• pp.98-107
• /
• 2017

MicroRNAs (miRNAs) act as regulators of gene expression by binding to the 3' untranslated region (UTR) of target genes. They perform important biological functions in the various species. Among many miRNAs, miR-21-3p is known to serve vital functions in development and apoptosis in olive flounder. Using genomic and bioinformatic tools, evolutionary conservation of miR-21-3p was examined in various species, and expression pattern was analyzed in olive flounder. Conserved sequences (5'-CAGUCG-3') in numerous species were detected through the stem-loop structure of miR-21-3p. Thus, we analyzed target genes of miR-21-3p. Among them, 3' UTR region of PPIL2 gene indicated the highest binding affinity with miR-21-3p based on the minimum free energy value. The PPIL2 gene showed high expression levels in testis tissue of the olive flounder, whereas miR-21-3p showed rather ubiquitous expression patterns except in testis tissue, indicating that miR-21-3p seems to control the PPIL2 gene expression in a complementary repression manner in various tissues of olive flounder. Taken together, this current study contributes to infer the target gene candidates for the miR-21-3p using bioinformatics tools. Furthermore, our data offers important information on the relationship between miR-21-3p and target gene for further functional study.