• Journal of Powder Materials
• /
• v.8 no.1
• /
• pp.20-25
• /
• 2001

Most of mold manufacturing procedures have been automated by the introduction of NC machine tool and CAD/CAM system. But the three-dimensional surface curvature of the mold must be done by hand work of well-skilled workers. Magnetic abrasive polishing powders were investigated for surface polishing for 3D curvature. This study aims to investigate homogeneously distributed hard phase in Fe matrix and strong bonding between Fe-matrix and hard phase. The NbC powder, $B_4C$ powder and $Al_2O_3$ powder were mixed in Fe-matrix respectively. Mixed Fe-hard phase powders were compacted by press and then these were melted by plasma melting. According to SEM, XRD and OM observation, Fe-NbC magnetic abrsive powder had the most homogeneous distribution and strong bonding. As a result of magnetic polishing, the surface roughness before magnetic polishing, 1 ${\mu}m$ $R_{max}$, was reduced to 0.2 ${\mu}m$ $R_{max}$ over the entire inner surface of the tube.

• Bulletin of the Korean Chemical Society
• /
• v.28 no.11
• /
• pp.2009-2014
• /
• 2007

The aminolysis of diphenyl thiophosphinic chloride (2) with substituted anilines in acetonitrile at 55.0 oC is investigated kinetically. Kinetic results yield large Hammett ρX (ρnuc = ?3.97) and Bronsted βX (βnuc = 1.40) values. A concerted mechanism involving a partial frontside nucleophilic attack through a hydrogen-bonded, four-center type transition state is proposed on the basis of the primary normal kinetic isotope effects (kH/kD = 1.0-1.1) with deuterated aniline (XC6H4ND2) nucleophiles. The natural bond order charges on P and the degrees of distortion of 42 compounds: chlorophosphates [(R1O)(R2O)P(=O)Cl], chlorothiophosphates [(R1O)(R2O)P(=S)Cl], phosphonochloridates [(R1O)R2P(=O)Cl], phosphonochlorothioates [(R1O)R2P(=S)Cl], chlorophosphinates [R1R2P(=O)Cl], and chlorothiophosphinates [R1R2P(=S)Cl] are calculated at the B3LYP/ 6-311+G(d,p) level in the gas phase.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.22 no.4
• /
• pp.75-80
• /
• 1995

It is important to estimate the possibility of recovery in physiologically damaged woody plant. It is suggested that C.E.R(cambial electrical resistance) might be a useful method to predict the permanent wilting point. D/A and A/D converter can be used to measure the C.E.R and it took only 10-20 msec for a measurement and the values were stable during this study. A computer could be used for the continual measurement of C.E.R. There were very big daily changes of C.E.R. was changed according to the changes of indoor temperature, but the phase was slightly different. It is reasoned that daily changes in C.E.R. is induced by the changes of water potential and cambial thickness. It was difficult to detect the changes of C.E.R. caused by changes in soil moisture under high soil water potential. Under low soil water potential, the changes in soil moisture under high soil water potential. Under low soil water potential, the changes of C.E.R. can be detected. After wilting, C.E.R. is increased very rapidly. When C.E.R. is not decreased by watering, it will be permanent wilting point. But it takes several days to confirm the permanent wilting point. To predict the possibility of recovery from wilting, the values of C.E.R. have no meaning. But the changes of C.E.R. are significant. Therefore we can predict the permant wilting point in woody plant by monitoring the change of C.E.R. by the computer.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.8
• /
• pp.2419-2424
• /
• 2013

Guanosine-5'-diphosphate 3'-diphosphate (ppGpp) serves as alarmone in bacterial stringent responses. In this study, an affinity column was constructed by immobilizing ppGpp to NHS-Sepharose for isolating ppGpp-binding proteins. A novel ppGpp-binding protein, YjgA, was isolated and characterized by MALDI-TOF MS (matrix-assisted laser desorption ionization-time-of-flight mass spectrometry) coupled with two-dimensional gel electrophoresis. YjgA and truncated forms of YjgA were cloned and over-expressed in BL21 (DE3). The binding affinity of YjgA to ppGpp was determined by equilibrium dialysis. The interaction of YjgA with ppGpp was very specific, considering that the dissociation constant of YjgA with ppGpp was measured as $5.2{\pm}2.0{\mu}M$, while the affinities to GTP and GDP were about 60 and 30 times weaker than ppGpp. Expression of yjgA gene in Escherichia coli K-12 MG1655 was examined by reverse transcription polymerase chain reaction (RT-PCR). RT-PCR results revealed that yjgA was expressed from early to late stationary phase. The yjgA deletion mutant exhibited decreased cell number at stationary phase compared to parent strain and the over-expression of YjgA increased the cell number. These results suggested that YjgA might stimulate cell division under stationary phase. In most prokaryotic genome, about half of the protein candidates are hypothetical, that are expected to be expressed but there is no experimental report on their functions. The approach utilized in this study may serve as an effective mean to probe the functions of hypothetical proteins.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.16 no.6
• /
• pp.610-617
• /
• 2011

This paper proposes a novel and direct dead-time compensation method of the 3-phase inverter using space vector pulse width modulation(SVPWM) topology. The proposed dead-time compensation method directly compensates the dead-time to the turn-on time of the effective voltage vector according to the current direction of the medium voltage reference. Each phase voltages are determined by the switching times of the effective voltage vectors, and the practical switching times have loss according to the current direction by the dead-time effect in the 3-phase inverter. The proposed method adds the dead-time to the switching time of the effective voltage vector according to the current direction, so it does not require complex d-q transform and controller to compensate the voltage error. The proposed dead-time compensation scheme is verified by the computer simulation and experiments of 3-phase R-L load.

• Proceedings of the Korean Society for Technology of Plasticity Conference
• /
• 2008.10a
• /
• pp.304-307
• /
• 2008

In order to evaluate the effect of permanent softening behavior on springback prediction, 2D-draw bending simulations were compared with experiments for friction stir welded DP590 steel sheets. To account fur the nonlinear hardening behavior, the combined isotropic-kinematic hardening law was utilized with and without considering the permanent softening behavior during reverse loading. Also, the non-quadratic orthotropic yield function, Yld2000-2d, was used to describe the anisotropic initial-yielding behavior of the base sheet while anisotropic properties of the weld zone were ignored for simplicity.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.22 no.3
• /
• pp.205-209
• /
• 2009

In this study, in order to develop the composition ceramics for lead-free ultrasonic motor, (1-x-0.09)$NaNbO_{3-x}BaTiO_3-0,09LiNbO_3$ ceramics were fabricated using a conventional mixed oxide process and their piezoelectric and dielectric characteristics were investigated according to the $BaTiO_3$ substitution. All the specimens showed orthorhombic phase structure without secondary phase, $BaTiO_3$ substitution enhanced density, dielectric constant(${\epsilon}_r$) and electromechanical coupling factor($k_p$), However, mechanical quality factor was deteriorated. Curie temperature of specimens was observed as about $380^{\circ}C$. At the $BaTiO_3$ substitution of 4 mol%, density, electromechanical coupling factor($k_p$), dielectric constant(${\epsilon}_r$) and piezoelectric constant($d_{33}$) of specimen showed the optimum value of $4.493g/cm^3$, 0.236, 175, 70 pC/N, respectively.

• Analytical Science and Technology
• /
• v.36 no.5
• /
• pp.205-215
• /
• 2023

In the present study, an analytical method was proposed for detecting trifluralin in aquatic products at trace concentrations. The method employed QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) and gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) as the sample preparation and measurement, respectively. The effect of the aqueous phase volume used in the QuEChERS was demonstrated, and the ratio of 10:10 (mL) between water and acetonitrile phase was used for 5 g of sample. Besides, dSPE using C18 and primary-secondary amine (PSA) was applied to remove the potential interferences from the food matrices, indicating no remarkable analyte loss. The linear range was built up from 0.50 ㎍ L^-1 to 3.0 ㎍ L^-1 (R² = 0.9993). Other criteria, i.e., repeatability (RSD_r = 0.86-1.96 %), reproducibility (RSD_R = 1.09-2.01 %), and recovery (over 90 %), were in accordance with Appendix F of AOAC (2016) for method performance. Although no trifluralin was detected for the commercial samples (fish, shrimp, and breaded shrimp), the spiked samples performed favorable recoveries and precision.

• Molecules and Cells
• /
• v.38 no.2
• /
• pp.130-137
• /
• 2015

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3'-UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3'-UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.23 no.12
• /
• pp.1380-1387
• /
• 2012

The transmit/receive(T/R) module is a key component in the active phased array system. The brick-type T/R module has been widely used and the miniaturization has been an important factor to get the flexibility of the system configuration. For the miniaturization, multi-function chips(MFC) having a common leg configuration are suitable to reduce the number of required MMICs and a high isolation between transmit and receive paths is necessary for the high gain T/R modules. In this work, we propose a bias timing scheme for the compact T/R module and show the optimum timing based on measurements, in order to improve the feed-back path loop problem and the consequent isolation problem of the common leg configuration. We have implemented high power(7 W/channel) and high T/R gain(35 dB transmit and 30 dB receive gains) within the half size($140{\times}80{\times}16mm^3$) of the conventional T/R modules.