• Title/Summary/Keyword: R&D Phase

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Study on the production and management of aquatic animals : direct detection of Edwardsiella tarda using an enzyme linked immunosorbent assay (수생산물의 생산과 관리에 관한 기초연구 : ELISA법을 이용한 Edwardssiella tarda의 직접 검출)

  • Chung, Soon-Yoon;Sohn, Sang-Gyu;Chung, Joon-Ki;Huh, Min-Do;Jeong, Hyun-Do
    • Journal of fish pathology
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    • v.10 no.2
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    • pp.75-86
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    • 1997
  • Solid phase enzyme linked immunosorbent assay (ELISA) was developed to detect the whole cells of Edwardsiella tarda from infected tissues of flounder. Cross-reaction test was performed by ELISA against fish pathogens such as A. hydrophila ATCC7966. V. anguillarum HYUFP5001, Y, ruckeri 11-4, E. ictaluri and Streptococcus sp. NG8206. Rabbit anti-E, tarda Edk-2 sera highly cross-reacted with A. hydrophila ATCC7966 and V. anguillarum HUFP5001. However, the cross-reaction was removed by using the anti-serum pre-adsorbed with A, hydrophila ATCC7966 FKC. The intra-species cross-reaction among E. tarda isolates was very high. ELISA with the whole cell antigens present in tissue homogenate appeared with highly decreased sensitivity, presumably by the co-coating of lipid or proteins in tissues. Thus, it would be necessary to use the infected tissue homogenates diluted more than 100 times with PBS for diagnosis. Interestingly, compared with the using of FKC antigen, the direct detection of viable cells in tissue homogenate showed more sensitive results with detection limit of $1{\times}10^3$ cells/ml in buffer or diluted tissue homogenate. Consequently, the ELISA method developed in this study was specific, rapid and sensitive for diagnosing edwardsiellosis.

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An Overview of the Herbal Remedies and other Non-conventional Therapies for 2009 Novel Influenza A(H1N1) (2009년 유행 신종인플루엔자 A(H1N1)의 한약 및 기타 비전형적 치료에 대한 고찰)

  • Sun, Seung-Ho;Jang, In-Soo;Baik, You-Sang;Bae, Sun-Jae;Han, Chang-Ho
    • The Journal of Internal Korean Medicine
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    • v.30 no.3
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    • pp.558-570
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    • 2009
  • Background : Since March 2009, when the first patient of novel influenza A (H1N1) was reported, many deaths have occurred in North and Central America. The start of the 2009 influenza pandemic was declared by WHO Director-General Dr. Margaret Chan on 11 June 2009, and the level of influenza pandemic alert raised from phase 5 to phase 6. There was no vaccine yet developed, and many experts worried that the novel H1N1 virus could kill as many or more as did the influenza pandemic in 1918-1919. Objective : To evaluate the possibility of treatment for 2009 novel influenza A (H1N1) using herbal remedies and other non-conventional therapies. Methods : We researched the clinical studies for novel H1N1 influenza virus-related herbal medicine or non-conventional medicine treatment using internet search engines including PubMed and CNKI. In addition, we reviewed many reports and clinical practice guidelines (CPG) for influenza A (H1N1). Results : Two case series were selected after reviewing 701 papers, and two CPG published by the Chinese government and Jilin province identified. They reported that the clinical symptoms were no more significant than seasonal influenza, and the condition of patients more than 45 years old was milder than those less than 45 years old. There are no patients with gastric problems, and oseltamivir has been used at the same time in all patients. Conclusion : The efficacy and effectiveness of herbal medicine and other non-conventional treatments for the novel influenza A (H1N1) is questionable, and more studies are needed to draw a firm conclusion. However, in the severe acute respiratory syndrome (SARS) experience in 2002/2003, it was demonstrated that herbal medicine can relieve all symptoms of SARS patients, promote absorption of lung inflammation, improve the degree of blood oxygen saturation, regulate immunological functions, reduce the required dosage of glucocorticoid and other medicines, and reduce case fatality rate. In light of the current situation that there is no vaccine or conventional treatment yet available, the study of herbal medicine and other non-conventional therapies are also necessary for appropriate evaluation.

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Effect of 3-Amino-1,2,4-triazole on Microstructure and Properties of Maleated HDPE/Maleated EPDM Blend (3-Amino-1,2,4-triazole이 Maleated HDPE/Maleated EPDM 블렌드의 미세구조 및 물성에 미치는 영향)

  • Kim, Tae Hyun;Chang, Young-Wook;Lee, Yong Woo;Kim, Dong Hyun
    • Elastomers and Composites
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    • v.49 no.1
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    • pp.24-30
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    • 2014
  • 3-Amino-1,2,4-triazole (ATA) (2.5 and 5.0 phr) was incorporated into a immiscible maleated ethylene propylene diene rubber(mEPDM)/maleated high density polyethylene(mHDPE) (50 wt%/50 wt%) blend by melt mixing. Effects of the ATA on structure, mechanical and rheological properties of the blend was investigated. FT-IR and DMA results revealed that supramolecular hydrogen bonding interactions between the polymer chains occur by reaction of ATA with maleic anhydride grafted onto the component polymers in the blend, which induces the physical crosslinks in the blend. FE-SEM analysis showed that mEPDM forms a dispersed phase in continuous mHDPE matrix, and the blend with the ATA has finer phase morphology as compared to the blend without the ATA. By the addition of ATA in the blend, there were significant increases in tensile strength, modulus and elongation-at-break as well as elastic recoverability. Melt rheology studies revealed that ATA induced substantial increase in storage modulus and complex viscosity of the blend at the melt state.

Production of Rhizobium meliloti M14 Inoculum by Semi-continuous Cultivation (반연속식(半連續式) 배양(培養)에 의(依)한 Rhizobium meliloti M14의 균체생산(菌體生産))

  • Choi, Woo Young;Sohn, Jong Rok;Kim, Moon Kyu
    • Korean Journal of Agricultural Science
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    • v.11 no.2
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    • pp.322-327
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    • 1984
  • As a basic studies for the laboratory scale production of alfalfa inoculum, Rhizobium meliloti M14 was characterized for its carbon and nitrogen sources, and some parameters for broth cultivation in a chemostat were studied by semi-continuous operation. The result s obtained were as follows. 1. Growth rate of the strain was increased by disaccharides than by monosaccharides tested, and pentoses resulted in poor growth than hexoses. Sugar alcohols including inositol supported the best growth among sugars. 2. Mannitol in the yeast-mannitol-broth was substituted by natural carbon sources such as malt extract or molasses. 3. Ten per cent of fresh yeast water appeared to supply enough amount of growth factor s for the strain, and the effect was equivalent to 0.24 percent of the commercial yeast extract powder. 4. Batch growth of the stain in a chemostat, New Brunswick Micro Ferm 28L, reached in the early stationary growth phase of $5{\sim}7{\times}10^9cells/ml$ after 36 hours of incubation. The culture at this stage was switched to semi-continuous cultivation, and the culture broth of four-fifth of the working volume was recovered every 24 hours when the maximal count was obtained.

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An Analytical Study on the Revegetation Methods for Highway Slopes (고속도로 절·성토 비탈면 녹화 공법의 적용 실태 연구)

  • Kim, Namchoon;Song, Hokyung;Park, Gwansoo;Jeon, Giseong;Lee, Sanghwa;Lee, Byungjoon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.2
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    • pp.1-15
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    • 2007
  • A variety of revegetation methods are being utilized and developed. However, most of revegetation methods used on highway slopes in Korea are based on foreign-introduced plant varieties to stabilize road surfaces and to administer afforestation for surface covering at an earlier phase. Therefore. it results in various problems. such as failure to achieve harmony with the surrounding vegetation and 10 cause re-denudation of slopes as the foreign-in introduced plant varieties wane out from 2~3 years after hydro-seeding, etc. In addition, some of the revegetation plants seeded in the earlier phase grow excessively high, thus causes successional problems, such as to inhibit the invasion of the secondary vegetation from the surrounding areas, etc. Therefore, in this study, 160 slopes located in the nationwide express highways have been investigated and analyzed in order to produce basic data for restoration of ecological environment in slopes created on a long-term basis by investigating and analyzing locational characteristics of cut and filled slopes in express highways, status of revegetation methods, characteristics of soil and plant-ecological environment. 1. Investigation on cut and embanked slopes in express highways was carried out in the total of 160 locations, which include 108 cut slopes and 52 embanked slopes. As a whole, the most frequently used revegetation method was seed spray, which was found to be used in the total of 55 target slops investigated. 2. Planting method of Wistaria floribunda applied to some of the blasted rock zones was found to cause damages as Wistaria floribunda trailed up the surrounding vegetation and the secondary invaded trees. In order to prevent this, this method must be used only in the lowest parts of large-sized slopes. Also, it will be required to administer continuous management and maintenance in the areas already planted with this plants. 3. The areas of blasted rock and ripping rock slopes were applied with coir net (net + seeding) method. However, many of these areas failed in achieving ground covering. Most areas where revegetation was in progress, they were covered with Eragrostis curvula(Weeping lovegrass) only. In areas with soil, such as decomposition of granite, where afforestation is difficult. In this slopes, soil base must be improved by hrdroseeding with thin-layer vegetation base application methods in order to achieve success in afforestation with native plants. 4. Woody species, rather than herb species, are more helpful in stabilization of slope surfaces. Therefore, it is important to be able to grow and protect woody species on highway slopes. Growth of woody vegetation is most largely influenced by soil depth. Thus, when hydro-seeding woody plants, it is recommended to apply at the upper layer of the slopes, which is capable to sufficiently provide the fundamentals required in plant growth.

Alkenylation of o-xylene with 1,3-Butadiene Over Base Catalysts (염기성 촉매를 이용한 o-xylene과 1,3-Butadiene의 알케닐화 반응)

  • Lee, Jong Seok;Lee, Soo Chool;Kil, Min Ho;Choi, Il Seok;Lee, Jae Sung;Kim, Jae Chang
    • Korean Chemical Engineering Research
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    • v.40 no.6
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    • pp.669-675
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    • 2002
  • The alkenylation of o-xylene with 1,3-butadiene to make OTP(ortho-tolyl Pentene) was carried out over liquid phase NaK alloy, Na metal and the metallic sodium dispersed on the specific support such as NaX and $Al_2O_3$. Liquid phase NaK alloy showed the improved conversion and selectivity when they were pretreated by ultrasound to increase the dispersion. For the case of metallic sodium, the induction period for the formation of homogeneous metal sodium solution with high dispersion was needed before the reaction. In the case of metallic sodium dispersed on support, more than 80 % conversion could be obtained without induction period regardless of supports used. But 85 % of the metallic sodium was resolved into the reaction mixture after reaction for 7 hours. The amount of byproducts, oligomers, produced from OTP and 1,3-butadiene increased with the amount of 1,3-butadiene introduced and the selectivity to OTP was in inversely proportional to the conversion.

TRIO (Triplet Ionospheric Observatory) CINEMA

  • Lee, Dong-Hun;Seon, Jong-Ho;Jin, Ho;Kim, Khan-Hyuk;Lee, Jae-Jin;Jeon, Sang-Min;Pak, Soo-Jong;Jang, Min-Hwan;Kim, Kap-Sung;Lin, R.P.;Parks, G.K.;Halekas, J.S.;Larson, D.E.;Eastwood, J.P.;Roelof, E.C.;Horbury, T.S.
    • Bulletin of the Korean Space Science Society
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    • 2009.10a
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    • pp.42.3-43
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    • 2009
  • Triplets of identical cubesats will be built to carry out the following scientific objectives: i) multi-observations of ionospheric ENA (Energetic Neutral Atom) imaging, ii) ionospheric signature of suprathermal electrons and ions associated with auroral acceleration as well as electron microbursts, and iii) complementary measurements of magnetic fields for particle data. Each satellite, a cubesat for ion, neutral, electron, and magnetic fields (CINEMA), is equipped with a suprathermal electron, ion, neutral (STEIN) instrument and a 3-axis magnetometer of magnetoresistive sensors. TRIO is developed by three institutes: i) two CINEMA by Kyung Hee University (KHU) under the WCU program, ii) one CINEMA by UC Berkeley under the NSF support, and iii) three magnetometers by Imperial College, respectively. Multi-spacecraft observations in the STEIN instruments will provide i) stereo ENA imaging with a wide angle in local times, which are sensitive to the evolution of ring current phase space distributions, ii) suprathermal electron measurements with narrow spacings, which reveal the differential signature of accelerated electrons driven by Alfven waves and/or double layer formation in the ionosphere between the acceleration region and the aurora, and iii) suprathermal ion precipitation when the storm-time ring current appears. In addition, multi-spacecraft magnetic field measurements in low earth orbits will allow the tracking of the phase fronts of ULF waves, FTEs, and quasi-periodic reconnection events between ground-based magnetometer data and upstream satellite data.

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Structural and Piezoelectric Properties of MnO2-Doped PZT-PSN Ceramics for Ultrasonic Vibrator (초음파 진동자용 MnO2가 Doping된 PZT-PSN 세라믹스의 구조 및 압전 특성)

  • Cha, Yoo-Jeong;Kim, Chang-Il;Kim, Kyoung-Jun;Jeong, Young-Hun;Lee, Young-Jin;Lee, Hai-Gun;Paik, Jong-Hoo
    • Korean Journal of Materials Research
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    • v.19 no.4
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    • pp.198-202
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    • 2009
  • For use in ultrasonic actuators, we investigated the structural and piezoelectric properties of $(1\;-\;x)Pb(Zr_{0.515}Ti_{0.485})O_3$ - $xPb(Sb_{1/2}Nb_{1/2})O_3$ + 0.5 wt% $MnO_2$ [(1 - x)PZT - xPSN + $MnO_2$] ceramics with a variation of x (x = 0.02, 0.04, 0.06, 0.08). All the ceramics, which were sintered at $1250^{\circ}C$ for 2 h, showed a typical perovskite structure, implying that they were well synthesized. A homogeneous micro structure was also developed for the specimens, and their average grain size was slightly decreased to $1.3{\mu}m$ by increasing x to 0.8. Moreover, a second phase with a pyrochlore structure appeared when x was above 0.06, which resulted in the deterioration of their piezoelectric properties. However, the 0.96PZT-0.04PSN+$MnO_2$ ceramics, which corresponds with a morphotropic phase boundary (MPB) composition in the (1 - x)PZT - xPSN + $MnO_2$ system, exhibited good piezoelectric properties: a piezoelectric constant ($d_{33}$) of 325 pC/N, an electromechanical coupling factor ($k_p$) of 70.8%, and a mechanical quality factor ($Q_m$) of 1779. The specimens with a relatively high curie temperature ($T_c$) of $305^{\circ}C$ also showed a significantly high dielectric constant (${\varepsilon}_r$) value of 1109. Therefore, the 0.96PZT - 0.04PSN + $MnO_2$ ceramics are suitable for use in ultrasonic vibrators.

Thermal Properties and Microencapsulation of a Phosphate Flame Retardant with a Epoxy Resin (에폭시 수지를 이용한 인계 난연제의 마이크로캡슐화 및 열적 특성 연구)

  • Baek Kyung-Hyun;Lee Jun-Young;Hong Sang-Hyun;Kim Jung-Hyun
    • Polymer(Korea)
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    • v.28 no.5
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    • pp.404-411
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    • 2004
  • The microcapsules containing triphenyl phosphate (TPP), a flame retardant, were prepared by phase-inversion emulsification technique using the epoxy resin (Novolac type) with excellent physical properties and network structure. This microencapsulation process was adopted for the protection of TPP evaporation and wetting of polymer composite during the polymer blend processing. The TPP, epoxy resin and mixed surfactants were emulsified to oil in water (O/W) by the phase inversion technology and then conducted on the crosslinking of epoxy resin by in-situ polymerization. The capsule size and size distribution of TPP capsules was controlled by mixed surfactant ratio, concentration and TPP contents, The formation and thermal property of TPP capsules were measured by differential scanning calorimetry and thermogravimetric analysis. The morphology and size of TPP capsules were also investigated by scanning and transmission electron microscopies. As the surfactant concentration increased, the TPP capsules were more spherical and mono-dispersed at the same weight ratio of mixed surfactants (F127: SDBS).

A Study to Improve the DC Output Waveforms of AFE Three-Phase PWM Rectifiers (AFE 방식 3상 PWM 정류기의 직류 출력파형 개선에 관한 연구)

  • Jeon, Hyeon-Min;Yoon, Kyoung-Kuk;Kim, Jong-Su
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.6
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    • pp.739-745
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    • 2017
  • Many studies have been conducted to reduce environmental pollution by ships and reduce fuel consumption. As part of this effort, research on power conversion systems through DC distribution systems that link renewable energy with conventional power grids has been pursued as well. The diode rectifiers currently used include many lower harmonics in the input current of the load and distort supply voltage to lower the power quality of the whole system. This distortion of voltage waveforms causes the malfunctions of generators, load devices and inverter pole switching elements, resulting in a large number of switching losses. In this paper, a controller is presented to improve DC output waveforms, the input Power Factor and the THD of an AFE type PWM rectifier used for PLL. DC output voltage waveforms have been improved, and the input Power Factor can now be matched to the unit power factor. In addition, the THD of the input power supply has been proven by simulation to comply with the requirements of IEEE Std514-2014.