• 대한약침학회지
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• 제26권4호
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• pp.307-318
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• 2023

Objectives: Bacterial biofilm is regarded as a significant threat to the production of safe food and the arise of antibiotic-resistant bacteria. The objective of this investigation is to evaluate the quorum sensing inhibitory effect of Nepeta curviflora methanolic extract. Methods: The effectiveness of the leaves at sub-inhibitory concentrations of 2.5, 1.25, and 0.6 mg/mL on the virulence factors and biofilm formation of P. aeruginosa was evaluated. The effect of N. curviflora methanolic extract on the virulence factors of P. aeruginosa, including pyocyanin, rhamnolipid, protease, and chitinase, was evaluated. Other tests including the crystal violet assay, scanning electron microscopy (SEM), swarming motility, aggregation ability, hydrophobicity and exopolysaccharide production were conducted to assess the effect of the extract on the formation of biofilm. Insight into the mode of antiquorum sensing action was evaluated by examining the effect of the extract on the activity of N-Acyl homoserine lactone (AHL) and the expression of pslA and pelA genes. Results: The results showed a significant attenuation in the production of pyocyanin and rhamnolipid and in the activities of protease and chitinase enzymes at 2.5 and 1.25 mg/mL. In addition, N. curviflora methanolic extract significantly inhibited the formation of P. aeruginosa biofilm by decreasing aggregation, hydrophobicity, and swarming motility as well as the production of exopolysaccharide (EPS). A significant reduction in AHL secretion and pslA gene expression was observed, indicating that the extract inhibited quorum sensing by disrupting the quorum-sensing systems. The quorum-sensing inhibitory effect of N. curviflora extract appears to be attributed to the presence of kaempferol, quercetin, salicylic acid, rutin, and rosmarinic acid, as indicated by LCMS analysis. Conclusion: The results of the present study provide insight into the potential of developing anti-quorum sensing agents using the extract and the identified compounds to treat infections resulting from quorum sensing-mediated bacterial pathogenesis.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2003년도 춘계학술발표논문집 (상)
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• pp.173-176
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• 2003

최근 분산 컴퓨팅 환경에서 데이터와 서비스의 복제는 통신비용의 감소, 데이터 가용성 증가, 그리고 단일 서버의 병목현상을 피하기 위해 필수적이다. 기존의 대표적인 복제 프로토콜로 네트워크를 논리적으로 구성하는 Tree quorum 프로토콜이 있다 Tree quorum프로토콜은 최선의 경우 가장 우수한 읽기 성능을 보이는 반면 트리의 높이가 증가할수록 노드의 수가 기하급수적으로 증가한다는 단점을 가지고 있다. 따라서 본 논문에서는 Tree quorum 프로토콜의 장점을 가지며, 급격한 노드 증가에 따른 성능 저하 문제를 해결하기 위한 가상 트리 프로토콜을 제안한다. 제안된 가상 트리 프로토콜은 안정된 삼각형 구조의 노드 구성과 가상 구조를 통한 Quorum 프로토콜의 사용으로 Tree quorum 프로토콜에 비해 적은 읽기 비용을 가지며, 적은 수의 노드 구성에서도 높은 읽기 가용성을 갖는다.

• 한국멀티미디어학회논문지
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• 제20권8호
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• pp.1369-1378
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• 2017

Pub/Sub (Publish/Subscribe) paradigm is a simple and easy to use model for interconnecting applications in a distributed environment. In general, subscribers register their interests in a topic or a pattern of events and then asynchronously receive events matching their interest, regardless of the events' publisher. In order to build a low latency lightweight pub/sub system for Internet of Things (IoT) services, we propose a GQSFPS (Group Quorum System-based Fog Pub/Sub) system that is a core component in the event-driven service oriented architecture framework for IoT services. The GQSFPS organizes multiple installed pub/sub brokers in the fog servers into a group quorum based P2P (peer-to-peer) topology for the efficient searching and the low latency accessing of events. Therefore, the events of IoT are cached on the basis of group quorum, and the delay-sensitive IoT applications of edge devices can effectively access the cached events from group quorum fog servers in low latency. The performance of the proposed GQSFPS is evaluated through an analytical model, and is compared to the GQPS (grid quorum-based pud/sub system).

• Journal of Microbiology and Biotechnology
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• 제24권3호
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• pp.401-407
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• 2014

Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ${\Delta}luxS$ (ECM101) and ${\Delta}luxS{\Delta}relA{\Delta}spoT$ (ECM201), and the ${\Delta}luxS$ complement strain to ECM201 (ECM202) were created from EHEC O157:H7 EDL933 to investigate how the regulatory systems interact. The phenotypic changes of the mutant strains were characterized and compared with the wild type. The mutant strains exhibited no obvious growth defects, although acid resistance and cellular cytotoxicity were decreased significantly in all the mutant strains. Phenotypic characterization revealed that mutations in the stringent response system (ECM201 and ECM202) influenced the metabolic (defective utilization of arabinose and L-sorbose) and enzymatic activities (decreased trypsin activity, and increased ${\alpha}$-glucosidase activity). In contrast, the quorum sensing system mutant (ECM101) did not display these phenotypes. The motility of the quorum sensing system mutant (ECM101) was unchanged, but mutation in the stringent response system influenced the motility. Our results suggest that quorum sensing interacts with the stringent response regulation system.

• 대한약침학회지
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• 제25권1호
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• pp.37-45
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• 2022

Objectives: The quorum-sensing-inhibitory and anti-biofilm activities of the methanol extract of E. globulus leaves were determined against clinically isolated multidrug-resistant Pseudomonas aeruginosa. Methods: The preliminary anti-quorum-sensing (AQS) activity of eucalyptus was investigated against a biosensor strain Chromobacterium violaceum ATCC 12472 (CV12472) by using the agar well diffusion method. The effect of sub-minimum inhibitory concentrations (sub-MICs) of the methanol extract of eucalyptus on different quorum-sensing-regulated virulence factors, such as swarming motility, pyocyanin pigment, exopolysaccharide (EPS), and biofilm formation, against clinical isolates (CIs 2, 3, and 4) and reference PA01 of Pseudomonas aeruginosa were determined using the swarm diameter (mm)-measurement method, chloroform extraction method, phenol (5%)-sulphuric acid (concentrated) method, and the microtiter plate assay respectively, and the inhibition (%) in formation were calculated. Results: The preliminary AQS activity (violacein pigment inhibition) of eucalyptus was confirmed against Chromobacterium violaceum ATCC 12472 (CV12472). The eucalyptus extract also showed concentration-dependent inhibition (%) of swarming motility, pyocyanin pigment, EPS, and biofilm formation in different CIs and PA01 of P. aeruginosa. Conclusion: Our results revealed the effectiveness of the E. globulus extract for the regulation of quorum-sensing-dependent virulence factors and biofilm formation at a reduced dose (sub-MICs) and suggest that E. globulus may be a therapeutic agent for curing and controlling bacterial infection and thereby reducing the possibility of resistance development in pathogenic strains.

• 한국정보과학회논문지:시스템및이론
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• 제31권9호
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• pp.503-513
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• 2004

최근 분산 컴퓨팅 환경에서 데이타와 서비스의 복제는 통신비용의 감소, 데이타 가용성 증가, 그리고 단일 서버의 병목현상을 피하기 위해 필수적이다. 기존의 대표적인 복제 프로토콜로 네트워크를 논리적으로 구성하는 Tree quorum 프로토콜과 Grid프로토콜이 있다. Tree quorum프로토콜은 최선의 경우 가장 우수한 읽기 성능을 보이는 반면 트리의 높이가 증가할수록 노드의 수가 기하급수적으로 증가한다는 단점을 가지고 있다. Grid프로토콜은 읽기 동작에 있어 높은 가용성을 가지는 반면 고장이 없는 환경에서도 같은 읽기 및 쓰기 성능을 보이는 단점을 가지고 있다. 따라서 본 논문에서는 기존의 복제 프로토콜이 가지는 문제점을 해결하고, 대칭적 트리 구조를 이용하여 노드의 장애가 발생하는 환경에서도 우수한 성능을 갖는 복제 프로토콜을 제안한다. 제안된 복제 프로토콜은 Tree quorum 프로토콜에 비해 적은 저기 비용을 가지며, 적은 수의 노드 구성 환경에서도 높은 읽기 가용성을 가진다. 또한 응답시간 면에서도 우수한 성능을 보인다.

• Journal of Microbiology and Biotechnology
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• 제17권10호
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• pp.1598-1606
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• 2007

A new series comprising 7 analogs of N-(sulfanyl ethanoyl)-L-HSL derivatives, 2 analogs of N-(fluoroalkanoyl)-$_L$-HSL derivatives, N-(fluorosulfonyl)-L-HSL, and 2,2-dimethyl butanoyl HSL were synthesized using a solid-phase organic synthesis method. Each of the 11 synthesized compounds was analyzed using NMR and mass spectroscopies, and molecular modeling studies of the 11 ligands were performed using SYBYL packages. Thereafter, a bacterial test was designed to identify their quorum-sensing inhibition activity and antifouling efficacy. Most of the synthesized compounds were found to be effective as quorum-sensing antagonists, where antagonist screening revealed that 10 among the 11 synthesized ligands were able to antagonize the quorum sensing of A. tumefaciens.

• 한국통신학회논문지
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• 제41권7호
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• pp.750-753
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• 2016

Quorum sensing (QS) is a bacterium-to-bacterium cell communication mechanism allowing bio-cell network construction but such mechanism is not well defined yet. We construct a QS-based multiple access network (MAN) and then numerically analyse its average uplink channel capacity as well as BER performance over diffusion-based 3-D molecular communication channels.

• 한국정보보호학회:학술대회논문집
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• 한국정보보호학회 2002년도 종합학술발표회논문집
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• pp.53-56
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• 2002

This paper discusses the quorum based algorithm for group mutual exclusion defined by Yuh-Jzer Joung. Group mutual exclusion[4,5,6] is a generalization of mutual exclusion that allows a resource to be shared by processes of the same group, but requites processes of different groups to use the resource in a mutually exclusive style. Joung proposed a quorum system, which he referred to as the surficial quorum system for group mutual exclusion and two modifications of Maekawa's algorithm[6]. He mentioned that when a process may belong to more than one group, the process must identify one of the groups it belongs when it wishes to enter CS(Critical Section). However, his solution didn't provide mechanism of identifying a group which maximizes the possibility to enter CS. In this paper, we provide a mechanism for identifying that each process belongs to which group.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.545-548
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• 2001

Microbiologically influenced corrosion (MIC) of metal is common in the natural environment and sulfate reducing bacteria are representative microorganisms for MIC. We found that biofilm fomlation by SRB on the metal surface might be controlled by quorum sensing, which is a cell density dependent regulation of cell metabolism. As cell free culture fluids (spent media) of Desulfovibrio vulgaris and D. desulfuricans were tested for quontrn sensing related test strains, it was found that spent media of two SRB induced increased luminescence of Vibrio harveyi BB886 (sensor 1+, sensor 2-) and BB170 (sensor 1-, sensor 2+). Quorum activities of D. vulgaris and D. desulfuricans appeared to be parallel to growth patterns, i.e., it was low in the lag phase, highly increased in the exponential phase, and reached maximum in the stationary phase. Interestingly, however, luminescence of V. harveyi BB886 and BB170 induced by a unit cell mass of the SHB showed a maximal peak in the late lag phase. Hence, it was suspected that quorum sensing of these two SHB play unknown roles in shifting cells from dormant to growth stages.