• 생약학회지
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• 제50권1호
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• pp.53-58
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• 2019

Morus alba fruit contains several anthocyanins and flavonoids like cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside. Extractions from Morus alba fruit were performed with five different compositions of ethanol/water [v/v]. High-Performance Liquid Chromatography (HPLC) was used for the validation and content determination of cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside. The contents of cyanidin-3-O-rutinoside in ethanol extracts (100%, 70%, 40%, 20% and 0%, ethanol/water [v/v]) were 1.150%, 0.822%, 0.749%, 0.663% and 0.597%, respectively. Quercetin-3-O-rutinoside contents of ethanol extracts (100%, 70%, 40%, 20% and 0%, ethanol/water [v/v]) were 0.167%, 0.161%, 0.159%, 0.155% and 0.096%, respectively. The highest contents of cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside were in the ethanol extract with 100% ethanol/water [v/v]. These HPLC analysis method could be used as basic data for standardization of functional food from Morus alba fruit.

• Natural Product Sciences
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• 제3권1호
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• pp.8-10
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• 1997

A new flavonol glycoside, quercetin 3-rhamnosyl $(1{\rightarrow}3)$ galactoside [5] was isolated from the leaves of M. africana. The known compounds kaempferol 3-rutinoside [1], 3'-O-methylquercetin 3-rutinoside [2], quercetin 3-rutinoside [3], and quercetin 3-rhamnosyl $(1{\rightarrow}6)$ galactoside [4] were also isolated for the first time from this plant. Their structures were determined by chemical and spectroscopic methods.

• Natural Product Sciences
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• 제9권1호
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• pp.7-9
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• 2003

The antioxidative constituents isolated from Hedyotis diffusa were identified as quercetin 3-O-${\beta}$-rutinoside (1) and quercetin 3-O-${\beta}$-glucoside (2). We also isolated asperuloside (3) from this plant. Identification was done based on spectroscopic analysis. Quercetin 3-O-${\beta}$-rutinoside was the stronger antioxidant than quercetin 3-O-${\beta}$-glycoside while asperuloside was inactive.

• International Journal of Industrial Entomology and Biomaterials
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• 제41권2호
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• pp.45-50
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• 2020

Morus alba has white and/or purple fruits with a very sweet taste and low acidity. Most Korean mulberry trees have purple fruits. However, Baekokwang is a unique mulberry genetic resource in Korea with white fruits. In this study, flavonoids contents of Baekokwang mulberry leaf and fruit were analyzed using ultrahigh performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS) technique. UPLC-DAD-QTOF/MS chromatogram showed that 15 flavonoids and 9 flavonoids were isolated and identified from the mulberry leaf and fruit. Total flavonoids contents of Baekokwang leaves and fruits were 812.7 mg and 35.0 mg, respectively. Baekokwang leaves had 4 major flavonoids including quercetin 3-O-(6"-O-malonyl) glucoside, 235.3 ppm, kaempferol 3-O-(6"-O-malonyl) glucoside, 132.3 ppm, kaempferol 3-O-rutinoside (nicotiflorin), 108.1 ppm, and quercetin 3-O-rutinoside (rutin), 103.8 ppm. Baekokwang fruits had 3 major flavonoids including quercetin 3-O-(6"-O-malonyl) glucoside, 13.0 ppm, quercetin 3-O-rutinoside (rutin), 7.8 ppm, and kaempferol 3-O-rutinoside (nicotiflorin), 5.7 ppm. From the above results, mulberry leaves have rich flavonoids compared to its fruits.

• 생약학회지
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• 제24권1호
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• pp.47-53
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• 1993

The seasonal variations of the major six flavonol glycosides(kaempferol 2,6-dirhamnosyl glucoside, quercetin 3-O-rutinoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-rutinoside, quercetin 3-O-coumaroyl glucorhamnoside and kaempferol 3-O-coumaroyl glucorhamnoside) in Ginkgo biloba leaves were investigated. The contents were determined by HPLC on reversed phase $C_{18}$ column. This result showed that the percentage of six flavonol glycosides decreased during the season from 1.57% in May to 0.39% in November. The content of each flavonol glycoside indicated a similar tendency to decrease. However, the contents of rutinosides of kaempferol, quercetin and isorhamnetin fluctuated markedly than those of coumaroyl glucorhamnosides of kaempferol and quercetin and kaempferol 2,6-dirhamnosyl glucoside.

• 생약학회지
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• 제33권4호통권131호
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• pp.263-266
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• 2002

From the herbs of this plant, six compounds were isolated and their structures were characterized as dieicosyl ether (1), scopoletin (2), ${\alpha}-spinasterol-3-O-{\beta}-D-glucopyranoside$ (3), kaempferol (4), quercetin (5), and quercetin 7, 3', 4'-trimethylether-3-O-rutinoside (6) by chemical and spectroscopic evidences. These compounds were isolated for the first time from this plant and compound 6 was first isolated from nature.

• Journal of the Korean Wood Science and Technology
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• 제32권1호
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• pp.59-66
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• 2004

노간주나무 잎을 채취하여 건조시킨 후 분말로 제조하여 1.5 kg을 아세톤-물(7:3, v/v)로 추출하고 헥산, 메틸렌클로라이드, 에틸아세테이트 및 수용성으로 분획하여 동결 건조시켰다. 그 중에서 에틸아세테이트용성과 수용성 분획을 Sephadex LH-20으로 충진한 칼럼에서 메탄올과 에탄올-헥산 혼합액을 용리용매로 사용하여 칼럼크로마토그래피를 실시하였다. 단리된 화합물들은 TLC로 확인한 후 NMR스펙트럼을 사용하여 정확한 구조를 규명하였고 FAB 및 EI-MS로써 분자량을 측정하였다. 주로 많은 양의 (+)-catechin, quercetin-3-O-α-L-rhamnopyranoside와 isoconiferin이 분리되었으며, 소량의 umbelliferone과 quercetin-3-O- β-D-rutinoside도 분리되었다. 각 분획물들과 단리된 화합물들은 DPPH 라디칼 소거법을 이용하여 항산화실험을 실시하였으며, 분획물중에는 에틸아세테이트용성이 그리고 화합물중에는 (+)-catechin과 quercetin-3-O-α-L-rhamnopyranoside이 우수한 항산화 효능이 있는 것으로 나타났다.

• 대한한의학방제학회지
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• 제12권1호
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• pp.255-262
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• 2004

The inhibitory effect of the aerial parts of Fagopyrum esculentum on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was examined. The n-butanol fraction from the methanol extract of title plant showed stronger inhibitory effect than other fractions on DPPH radical. Two flavonoids were isolated from n-butanol fraction having the potent activity and elucidated as quercetin-3-O-${\alpha}$-L-rhamnoside and quercetin-3-O-rutinoside on the basis of spectral evidence. The $IC_{50}$ values of these compounds on DPPH radical were 6.56 ${\mu}M$ and 8.37 ${\mu}M$, respectively.

• 생약학회지
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• 제23권3호
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• pp.126-131
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• 1992

Four flavonoids were isolated from the aerial parts of Euphorbia ebracteolata. On the basis of chemical and spectroscopic evidence, the structures of these compounds were established as $quercetin-3-O-{\beta}-D-glucoside$(isoquercitrin)(I), quercetin-3-O-rutinoside(rutin)(II), kaempferol-3-O-rutinoside(III) and $quercetin-3-O-(2'-O-galloyl)-{\beta}-D-glucoside$(IV) which was the main flavonoidal component in this plant. The isolation of flavonoids from E. ebracteolata is the first example.

• 약학회지
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• 제40권2호
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• pp.170-176
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• 1996

A chemical examination of the aerial parts of Euphorbia pekinensis $R_{UPRECHT}$. (Euphorbiaceae) has led to the isolation of seven hydrolyzable tannins and ten fl avonoid glycosides. The former ones have been identified as gallic acid, methylgallate, 3-O-galloyl shikimic acid, 1,3,4,6-tetra-O-galloyl-${\beta}-_D$-glucose, 1,2,3,4,6-penta-O-galloyl-${\beta}-_D$-glucose, corilagin, geraniin and the latter ones as isoquercitrin, quercitrin, astragalin, afzelin, prunin, rutin, kaempferol-3-O-rutinoside, quercetin-3-O-(2"-O-galloyl)-${\beta}-_D$-glucoside and quercetin-3-O-(2"-O-galloyl)-${\alpha}-_L$-rhamnoside on the basis of chemical and spectroscopic evidence.