• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.113-113
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• 2017

Downy mildew (DM), caused by several species in the Peronosclerospora and Scleropthora genera, is a major maize (Zea mays L.) disease in tropical or subtropical regions. DM is an obligate parasite species in the higher plants and spreads by oospores, wind, and mycelium in seed surface, soil, and living hosts. Owing to its geographical distribution and destructive yield reduction, DM is one of the most severe maize diseases among the maize pathogens. Positional cloning in combination with phenotyping is a general approach to identify disease resistant gene candidates in plants; however, it requires several time-consuming steps including population or fine mapping. Therefore, in the present study, we suggest a new combination strategy to improve the identification of disease resistant gene candidates. Downy mildew (DM) resistant maize was selected from five cultivars using the spreader row technique. Positional cloning and bioinformatics tools identified the DM resistant QTL marker (bnlg1702) and 47 protein coding genes annotations. Eventually, 5 DM resistant gene candidates, including bZIP34, Bak1, and Ppr, were identified by quantitative RT-PCR without fine mapping of the bnlg1702 locus. Specifically, we provided DM resistant gene candidates with our new strategy, including field selection by the spreader row technique without population preparation, the DM resistance region identification by positional cloning using bioinformatics tools, and expression level profiling by quantitative RT-PCR without fine mapping. As whole genome information is available for other crops, we propose applying our novel protocol to other crops or for other diseases with suitable adjustment.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.16 no.1 s.106
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• pp.40-49
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• 2006

A novel disturbance measurement method, model based disturbance observer (MBDO) for optical disk drives (ODDs), is proposed and the disturbance analysis using the proposed method is performed under various conditions. In ODDs, the quantitative and qualitative analysis for the generated disturbance during normal operation is very important to successful servo loop design. However, the disturbance measurement is difficult, and high precision measurement is necessary. Furthermore, the conventional disturbance measurement method using a LDV (laser Doppler vibrometer) has many difficulties in eccentricity direction due to the vertical movement of an optical disk. To solve this problem, the MBDO is proposed. First, the relationship between the servo loop for ODDs and the generated disturbance are briefly reviewed. Second, the principle of the MBDO is introduced, and the disturbance measurement results, which are measured by the MBDO and a LDV, are compared. In these experiments, test DVD-ROM disks are used to generate quantitative/qualitative disturbance. Then, the disturbance analysis under various conditions is performed using waterfall technique. This technique clearly shows the disturbance trend from the inner part of an optical disk to the outer part of it. Finally, the various disturbances measurement results are summarized and some remarks for it are commented.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.6
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• pp.57-68
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• 2009

Recently, damage scale of human-induced disaster is sharply increased but its occurrences and damages are so uncertain that it is hard to construct a resonable response & mitigation plan for infrastructures. Therefore, the needs for a advanced risk management technique based on a probabilistic and stochastic risk evaluation theory is increased. In this study, these evaluation methods were investigated and a advanced disaster risk evaluation method, which is based on the probabilistic or stochastic risk assessment theory and also is a quantitative evaluation technique, was suggested. With this method, the safety changes as the result of fire damage management for recent 40 years was analyzed. And the result was compared with that of Japan. Through the consilience of the traditional risk assessment method and this method, a stochastical estimation technique for the uncertainty of future disaster's damage could support a cost-effective information for a resonable decision making on disaster mitigation.

• Korean Journal of Construction Engineering and Management
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• v.17 no.4
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• pp.40-48
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• 2016

The construction industry has been made diversified on the design process depending on qualitative growth of customers' demands. But this approach has lead to problems such as falling of building values due to lack of awareness of defects caused by long term utilization. So, the relationship on the characteristics of buildings and defects should be clearly analyzed to prevent falling of building values. This study, therefore, proposed a technique to quantify the relationship between building facade elements and defects. The technique was developed by applying pixel concept to the outside of the buildings. It has a feature to determine the clear relationship by presenting quantitative data that have been recognized qualitatively. The proposed technique is referred to "Pixelization Method". It separates building facade into unit compartment and makes database by assigning a code depending on the characteristics. Through the method, this study is expected to create a foundation for the quantitative analysis of relationship between building facade elements and defects as a basis on active responding to the defects.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.11
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• pp.1069-1076
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• 2007

This paper presents the road simulator control technology for reproducing the road input signal to implement the real road data. The simulator consists of the hydraulic pump, servo valve, hydraulic actuator and its control equipment. The QFT(Quantitative Feedback Theory) is utilized to control the simulator effectively. The control system illustrates a tracking performance of the closed-loop controller with low order transfer function G(s) and pre-filter F(s) for a parametric uncertain model. A force controller is designed to communicate the control signal between simulator and digital controller. Tracking specification is satisfied with upper and lower bound tolerances on the steep response of the system to the reference signal. The efficacy of the QFT force controller is verified through the numerical simulation, in which combined dynamics and actuation of the hydraulic servo system are tested. The simulation results show that the proposed control technique works well under uncertain hydraulic plant system. The conventional software (Labview) is used to make up for the real controller in the real-time basis, and the experimental works show that the proposed algorithm works well for a single road simulator.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.3
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• pp.280-289
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• 2008

This paper presents design of the quantitative feedback control system of the three axes hydraulic road simulator with respect to the dummy wheel for uncertain multiple input-output(MIMO) feedback systems. This simulator has the uncertain parameters such as fluid compressibility, fluid leakage, electrical servo components and nonlinear mechanical connections. This works have reproduced the random input signal to implement the real road vibration's data in the lab. The replaced $m^2$ MISO equivalent control systems satisfied the design specifications of the original $m^*m$ MIMO control system and developed the mathematical method using quantitative feedback theory based on schauder's fixed point theorem. This control system illustrates a tracking performance of the closed-loop controller with low order transfer function G(s) and pre-filter F(s) having the minimum bandwidth for parameters of uncertain plant. The efficacy of the designed controller is verified through the dynamic simulation with combined hydraulic model and Adams simulator model. The Matlab simulation results to connect with Adams simulator model show that the proposed control technique works well under uncertain hydraulic plant system. The designed control system has satisfied robust performance with stability bounds, tracking bounds and disturbance. The Hydraulic road simulator consists of the specimen, hydraulic pump, servo valve, hydraulic actuator and its control equipments

• Mass Spectrometry Letters
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• v.2 no.3
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• pp.69-72
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• 2011

Defective synthesis of the steroid hormones by the adrenal cortex has profound effects on human development and homeostasis. Due to the time-consuming chromatography procedure combined with mass spectrometry, the matrix-assisted laser desorption ionization method coupled to the linear ion-trap tandem mass spectrometry (MALDI-LTQ-MS/MS) was developed for quantitative analysis of 10 adrenal steroids in human serum. Although MALDI-MS can be introduced for its applicability as a high-throughput screening method, it has a limitation on reproducibility within and between samples, which renders poor reproducibility for quantification. For quantitative MALDI-MS/MS analysis, the stable-isotope labeled internal standards were used and the conditions of crystallization were tested. The precision and accuracy were 3.1~35.5% and 83.8~138.5%, respectively, when a mixture of 10 mg/mL ${\alpha}$-cyano-4-hydroxycinnamic acid in 0.2% TFA of 70% acetonitrile was used as the MALDI matrix. The limit of quantification ranged from 5 to 340 ng/mL, and the linearity as a correlation coefficient was higher than 0.988 for all analytes in the calibration range. Clinical applications include quantitative analyses of patients with congenital adrenal hyperplasia. The devised MALDI-MS/MS technique could be successfully applied to diagnosis of clinical samples.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.170-180
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• 2008

Successful design, construction and maintenance of NATM tunnel demands prediction, control, stability guidelines, the estimation pre-improvement support system and monitoring of surface settlement, gradient and ground displacement with high accuracy. Moreover, urban NATM tunnel under difficult geotechnical conditions is important the estimation and necessary of pre-improvement support system. Various strategies have been proposed for the quantitative estimation of pre-improvement support system. This paper was investigated and analysed an assessment technique for the quantitative estimation of pre-improvement support system on underground space, as mountain and urban tunnel, in detail. The analysis performed on design and construction stage with field database using the proposed stability estimation index by many researcher including the critical strain and the apparent Young's modulus concept.

• Proceedings of the KIEE Conference
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• 2005.10b
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• pp.133-137
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• 2005

In this paper, we investigate visual and quantitative analysis of different tastes in the liquids using multi-array chemical sensor (MACS) based on the ion-selective electrodes (ISEs), which is so called the electronic tongue (E-Tongue) system. We apply the Fuzzy C-means (FCM) algorithm combined with Principal Component Analysis (PCA), which can be used to reduce multi-dimensional data to two- or three-dimensional data, to classify visually data patterns detected by E-Tongue system. The proposed technique can be determined the cluster centers and membership grade of patterns through the unsupervised way. The membership grade of an unknown pattern, which does not shown previously, can be visually and analytically determined. Throughout the experimental trails, the E-tongue system combined with the proposed algorithms is demonstrated robust performance for visual and quantitative analysis for different tastes in the liquids.

• Proceedings of the KOSOMBE Conference
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• v.1997 no.11
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• pp.39-43
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• 1997

Posturography stands or quantitative assessment of body postural stability analysis. The present study developed a balance plate system to monitor patient's center of pressure (COP) movement and to analyze its stability. An equilateral triangular shaped plate was made of duralumin and forces were measured on the three vertices of the plate using industrial load cells. Specially designed electronic circuit picked up force signals ed into data acquisition system to calculate the cartesian coordinates of COP. COP calculation error was less than 2%. The force signals enabled to compute stability measures, which consisted of a variety of clinical parameters related to postural stability. Clinical experiments were carefully designed and performed on 40 normal subjects. The results were that 1) postural stability decreased with age and 2) the best parameters were those of posture deviation measures. A customized PC-based software package was developed to apply the present technique with a great convenience to monitoring and analyzing postural stability in an accurate and quantitative way.