• Title/Summary/Keyword: Quantitative Research

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EEG Recording Method for Quantitative Analysis (정량적 분석을 위한 뇌파 측정 방법)

  • Heo, Jaeseok;Chung, Kyungmi
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.4
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    • pp.397-405
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    • 2019
  • Quantitative electroencephalography (QEEG) has been widely used in research and clinical fields. QEEG has been widely used to objectively document cerebral changes for the purpose of identifying the electrophysiological biomarkers across various clinical symptoms and for the stimulation of specific cortical regions associated with cognitive function. In electroencephalography (EEG), the difference in quantitative and qualitative analyses is discriminated not by its measurement methods and relevant clinical or research environments, but by its analysis methods. When performing a qualitative analysis, it is possible for a medical technologist or experienced researchers to read the EEG waveforms to exclude artifacts. However, the quantitative analysis is still based on mathematical modeling, and all EEG data are included for the analysis, leading the results to be affected by unexpected artifacts. In the hospital setting, the case that the medical technologists in charge of the EEG test perform academic research has been little reported, compared to other clinical physiological measurement-based research. This is because there are few laboratories specialized in clinical physiological research. In this respect, this study is expected to be utilized as a basic reference material for medical technologists, students, and academic researchers, all of whom would like to conduct a quantitative analysis.

A Study on Liquified Petroleum Gas(LPG) Fuel Quantitative Method using Coriolis Mass Flowmeter (코리올리 질량유량계를 이용한 액화석유가스(LPG) 정량 측정 방법 연구)

  • Park, Tae-Seong;Seong, Sang-Rae;Yim, Eui-Soon;Lee, Joung-Min;Lee, Myung-Sig;Kang, Hyung-Kyu
    • Journal of the Korean Institute of Gas
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    • v.22 no.6
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    • pp.109-122
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    • 2018
  • Domestic LPG meters are being tested for LPG quantification in accordance with the "Measures Act". The LPG meter is re-tested every three years in accordance with the "Enforcement Decree of the Measures Act". The maximum permissible error within the test is within ${\pm}1.0%$, and the tolerance is within ${\pm}1.5%$. For the quantitative measurement of LPG, a hydrometer for LPG, a balance, and a pressure vessel are used. The volume of LPG varies in depending on the temperature and pressure. The current quantitative measurement method of LPG requires the measurement of temperature, pressure and density in order to determine the volume of LPG, respectively, and some equipments are needed accordingly. Coriolis mass flowmeter, on the other hand, measure the mass flow, density and temperature at the same time, and can be converted and calculated to the required values using a computer program, also it is widely applied in the industrial field. In this study, the volume of LPG was measured using a Coriolis mass flowmeter as a basic study of LPG quantitative measurement. In addition, it is shown that it is possible to apply for the LPG quantitative measurement using the Coriolis mass flowmeter by comparing it with the conventional LPG quantitative measurement method.

Study on the analytical method using GC-MS for the accident preparedness substances (실험실 GC-MS를 이용한 사고대비물질 분석방법 연구)

  • Kim, Kijoon;Lee, Jinseon;Lee, Suyeong;Hwang, Seungryul;Kim, Younghee;Seok, Gwangseol
    • Analytical Science and Technology
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    • v.26 no.1
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    • pp.80-85
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    • 2013
  • The sixty nine accident preparedness substances (APS) having high probability of chemical accident are controlled under the Toxic Chemicals Control Act (TCCA). Around the world, there has been a growing interest in the analysis of chemical warfare agent (CWAs). When a chemical accident occurs, it is generally required to detect and identify APS. However, the quantitative analytical data remain limited in Korea. In this study, an analytical method using GC/MS for volatile organic chemicals was established and a quantitative analysis method was studied. The calibration curve for 25 chemicals were obtained and 21 chemicals showed higher coefficient of determination ($r^2$ >0.998).

Collaborative Action Research: A Case in Korean Earth Science Classrooms

  • Oh, Phil-Seok
    • Journal of the Korean earth science society
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    • v.26 no.4
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    • pp.317-328
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    • 2005
  • This study is a report of the collaborative action research which has been conducted between a Korean earth science teacher and science education researcher. A two-year long action research effort was made in order to improve the teacher’s earth science classrooms in pursuit of constructivist principles of learning. The process of the action research was described with the aim of increasing the awareness of science teachers and science education researchers regarding action research. Quantitative evidence was presented to indicate the effectiveness of the collaborative action research in creating constructivist learning environments in the classrooms. The process and quantitative evidence from the action research permitted a consideration of implications for future efforts to improve science classrooms.

A Novel Marker for the Species-Specific Detection and Quantitation of Shigella sonnei by Targeting a Methylase Gene

  • Cho, Min Seok;Ahn, Tae-Young;Joh, Kiseong;Kwon, Oh-Sang;Jheong, Won-Hwa;Park, Dong Suk
    • Journal of Microbiology and Biotechnology
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    • v.22 no.8
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    • pp.1113-1117
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    • 2012
  • Shigella sonnei is a causal agent of fever, nausea, stomach cramps, vomiting, and diarrheal disease. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection of S. sonnei using a primer pair based on the methylase gene for the amplification of a 325 bp DNA fragment. The qPCR primer set for the accurate diagnosis of Shigella sonnei was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.

Quantitative evaluation of the molecular marker using droplet digital PCR

  • Shin, Wonseok;Kim, Haneul;Oh, Dong-Yep;Kim, Dong Hee;Han, Kyudong
    • Genomics & Informatics
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    • v.18 no.1
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    • pp.4.1-4.6
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    • 2020
  • Transposable elements (TEs) constitute approximately half of Bovine genome. They can be a powerful species-specific marker without regression mutations by the structure variation (SV) at the time of genomic evolution. In a previous study, we identified the Hanwoo-specific SV that was generated by a TE-association deletion event using traditional PCR method and Sanger sequencing validation. It could be used as a molecular marker to distinguish different cattle breeds (i.e., Hanwoo vs. Holstein). However, PCR is defective with various final copy quantifications from every sample. Thus, we applied to the droplet digital PCR (ddPCR) platform for accurate quantitative detection of the Hanwoo-specific SV. Although samples have low allele frequency variation within Hanwoo population, ddPCR could perform high sensitive detection with absolute quantification. We aimed to use ddPCR for more accurate quantification than PCR. We suggest that the ddPCR platform is applicable for the quantitative evaluation of molecular markers.

Screening Analysis of 10 Adrenal Steroids by Matrix-Assisted Laser Desorption Ionization-Tandem Mass Spectrometry

  • Kim, Sun-Ju;Jung, Hyun-Jin;Chung, Bong-Chul;Choi, Man-Ho
    • Mass Spectrometry Letters
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    • v.2 no.3
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    • pp.69-72
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    • 2011
  • Defective synthesis of the steroid hormones by the adrenal cortex has profound effects on human development and homeostasis. Due to the time-consuming chromatography procedure combined with mass spectrometry, the matrix-assisted laser desorption ionization method coupled to the linear ion-trap tandem mass spectrometry (MALDI-LTQ-MS/MS) was developed for quantitative analysis of 10 adrenal steroids in human serum. Although MALDI-MS can be introduced for its applicability as a high-throughput screening method, it has a limitation on reproducibility within and between samples, which renders poor reproducibility for quantification. For quantitative MALDI-MS/MS analysis, the stable-isotope labeled internal standards were used and the conditions of crystallization were tested. The precision and accuracy were 3.1~35.5% and 83.8~138.5%, respectively, when a mixture of 10 mg/mL ${\alpha}$-cyano-4-hydroxycinnamic acid in 0.2% TFA of 70% acetonitrile was used as the MALDI matrix. The limit of quantification ranged from 5 to 340 ng/mL, and the linearity as a correlation coefficient was higher than 0.988 for all analytes in the calibration range. Clinical applications include quantitative analyses of patients with congenital adrenal hyperplasia. The devised MALDI-MS/MS technique could be successfully applied to diagnosis of clinical samples.

Quantitative Analysis of Microencapsulation of $\beta$-Galactosidase (유당분해효소의 미세캡슐화 수율 측정)

  • Shin, Myung Gon;Chang, Pahn Shick;Min, Bong Kee;Kwak, Hae Soo
    • Analytical Science and Technology
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    • v.5 no.4
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    • pp.471-475
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    • 1992
  • We have developed the methodology for the quantitative analysis of microencapsulation yield and optimized the conditions for the microencapsulation of ${\beta}$-galactosidase by butter oil. The degree of ${\beta}$-galactosidase deactivation by the microencapsulation process was the value of 5.2% of initial activity. And the yield for the microencapsulation of ${\beta}$-galactosidase by the indirect, heat treatment, and enzymatic methodology were 92.6%, 88.6%, and 94.1%, respectively.

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Mapping of Quantitative Trait Loci Affecting Growth Traits in a Japanese Native Chicken Cross

  • Rikimaru, K.;Sasaki, O.;Koizumi, N.;Komatsu, M.;Suzuki, K.;Takahashi, Hideaki
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.10
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    • pp.1329-1334
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    • 2011
  • The Hinai-dori is a breed of chicken native to Akita Prefecture, Japan. An $F_2$ resource population produced by crossing low- and high-growth lines of the Hinai-dori breed was analyzed to detect quantitative trait loci (QTL) for growth traits. Highly significant QTLs for body weight at 10 and 14 weeks of age and average daily gain between 4 and 10 weeks and between 10 and 14 weeks of age were accordingly mapped in a common region between ADL0198 and ABR0287 on chromosome 1 and between MCW0240 and ABR0622 on chromosome 4, respectively. A significant QTL for body weight at 4 weeks of age and a significant QTL for average daily gain between 0 and 4 weeks of age were mapped for the first time to the same region flanking ABR0204 and ABR0284 on chromosome 1. These QTLs are good candidates for application in the development of marker-assisted selection strategies for increasing growth efficiencies in the Hinai-dori breed and native breeds of chickens in Asia.

Customer Electronic Loyalty towards Online Business: The role of Online Trust, Perceived Mental Benefits and Hedonic Value

  • NGUYEN, Minh Ha;KHOA, Bui Thanh
    • Journal of Distribution Science
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    • v.17 no.12
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    • pp.81-93
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    • 2019
  • Purpose: The success of electronic commerce businesses is the ability to retain the customers and inspire their loyalty in online shopping. The purpose of this study is to develop a model to study the effect of perceived mental benefits, online trust, and hedonic value on the elements of electronic loyalty. Research design, data and methodology: Mixed research method was applied in this study with qualitative and quantitative research method. Qualitative data was collected through focus group discussion with electronic commerce experts. Quantitative data was collected through a survey of 917 customers, in which conducted in four cities and one province in Vietnam. SmartPLS software is used for processing quantitative data. Results: The study points out that four constructs of the mental benefit concept, although not entirely, have an impact on online trust and hedonic value. At the same time, two antecedents of electronic loyalty's three elements are online trust and hedonic value. Conclusions: Through the positive influence between the elements in the conceptual model, the study has shown that the perceived mental benefits, online trust, and hedonic value are important factors to shape the electronic loyalty in developing countries, such as Vietnam. This study proposed some scientific and managerial implications.