• 한국안전학회지
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• 제24권5호
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• pp.69-76
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• 2009

The estimation of costs from industrial accidents is very important because they have a serious effect on individuals, companies, and nation. The department of labor estimates the cost of accidents by using the "Heinrich" method. From that method, the scale of accident cost can be approximately computed, but accurate calculation of uninsured cost is not easy. Therefore, a better method of calculating uninsured cost caused by industrial accident is necessary. This study aimed to construct an estimation method of uninsured cost according to domestic circumstances. The results of this study are as follows: (1) This study derived applicable factors for quantitative estimation of industrial accident cost (2) This study made the equation that the calculation of each item of uninsured cost was possible (3) This study applied the uninsured cost by degrees of disaster to individual items (4) The subjects and types of occurrence in uninsured cost were analyzed and presented. Theses results will provide a basis for further researchers of uninsured cost.

• 한국습지학회지
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• 제19권1호
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• pp.112-121
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• 2017

본 연구는 레이더의 중첩관측영역을 활용한 정량적 강수량 추정방법을 제안하였다. 이를 위해 국내의 조밀한 지상우량계 관측망과 레이더 관측망의 중첩관측영역을 이용하였다. 결과적으로 중첩관측영역 내 레이더간의 계통오차를 확인하였으며, 이를 이용하여 새로운 정량적 강수량 추정방법을 개발하였다. 이 방법으로 추정된 강우강도는 기존의 정량적 강수량 추정방법보다 모든 강우강도 범위에서 강수량이 적절하게 추정되는 것으로 나타났다.

• Journal of Information Processing Systems
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• 제1권1호
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• pp.79-85
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• 2005

In this paper, we dealt with substantial asset analysis methodology applied to two-dimensional asset classification and qualitative evaluation method according to the business process. Most of the existent risk analysis methodology and tools presented classification by asset type and physical evaluation by a quantitative method. We focused our research on qualitative evaluation with 2-dimensional asset classification. It converts from quantitative asset value with purchase cost, recovery and exchange cost, etc. to qualitative evaluation considering specific factors related to the business process. In the first phase, we classified the IT assets into tangible and intangible assets, including human and information data asset, and evaluated their value. Then, we converted the quantitative asset value to the qualitative asset value using a conversion standard table. In the second phase, we reclassified the assets using 2-dimensional classification factors reflecting the business process, and applied weight to the first evaluation results. This method is to consider the organization characteristics, IT asset structure scheme and business process. Therefore, we can evaluate the concrete and substantial asset value corresponding to the organization business process, even if they are the same asset type.

• Journal of Microbiology and Biotechnology
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• 제32권8호
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• pp.976-981
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• 2022

Phenanthrenes are bioactive phenolic compounds found in genus Dioscorea, in which they are distributed more in peel than in flesh. Recent studies on phenanthrenes from Dioscorea sp. peels have revealed the potential for valuable biomaterials. Herein, an analytical method using high-performance liquid chromatography (HPLC) for quantitation of bioactive phenanthrenes was developed and validated. The calibration curves were obtained using the phenanthrenes (1-3) previously isolated from Dioscorea batatas concentrations in the range of 0.625-20.00 ㎍/ml with a satisfactory coefficient of determination (R²) of 0.999. The limit of detection (LOD) and the limit of quantification (LOQ) values of the isolated phenanthrenes ranged from 0.78-0.89 and 2.38-2.71 ㎍/ml, respectively. The intraday and interday precision ranged from 0.25-7.58%. The recoveries of the isolated phenanthrenes were from 95 to 100% at concentrations of 1.25, 2.5, and 5.0 ㎍/ml. Additionally, phenanthrenes (1-3) were found in all investigated peel extracts. Hence, the developed method was encouraging for the quantitative analysis of phenanthrenes in genus Dioscorea.

• 한국식품저장유통학회지
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• 제30권3호
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• pp.383-394
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• 2023

The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was developed and validated to differentiate the morphologically similar ones, Oncorhynchus keta and Oncorhynchus mykiss. Species-specific primers were designed for the COI genes of mtDNA. The species-specific primers designed for O. keta and O. mykiss were selectively amplified by O. keta and O. mykiss DNA, respectively. The sensitivity of O. keta and O. mykiss primers was 1 ng/μL. Quantitative testing showed that the results met the 'Guidelines on Standard Procedures for Preparing Analysis Method such as Food' proposed by the Ministry of Food and Drug Safety. The qPCR method developed and validated in this study for identifying O. keta and O. mykiss has advantages such as speed and field applicability. Therefore, this method is expected to help control forgery and alteration of raw materials in the seafood industry.

• Journal of Veterinary Science
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• 제22권3호
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• pp.40.1-40.12
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• 2021

Background: The microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen. Objectives: The present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees. Methods: A procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration. Results: UR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 10⁴ spores/bee, and the stable detection level was ≥ 2.40 × 10⁵ spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 10⁴ copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min. Conclusions: UR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2935-2940
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• 2007

To diagnose circulatory diseases in the viewpoint of hemodynamics, we need to get quantitative hemodynamic information of blood flows related with the vascular diseases with high spatial resolution of tens micrometer and high temporal resolution in the order of millisecond. For investigating in-vivo hemodynamic phenomena, a new diagnosing technique combining medical radiography and PIV method was newly proposed and developed. This angiographic PIV technique consists of a medical X-ray tube, an X-ray CCD camera, a shutter module for double pulses of X-ray, and a synchronizer. The feasibility of the angiographic PIV technique was tested and quantitative flow velocity field distribution of a flow inside an opaque conduit was acquired by the developed system. It can be used for measuring flow phenomena of nontransparent fluids inside opaque conduits.

• 한국작물학회지
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• 제51권spc1호
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• pp.103-106
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• 2006

Anthocyanins are water-soluble glycosides and acylglycosides of anthocyanidins, having different color variations due to its substitution patterns. Anthocyanins, present in various fruits, vegetables and crops as natural colorant, have been well characterized for its bioactive properties, anti-oxidant, anti-cancer, anti-proliferative and anti-inflammatory properties. During extraction and purification, the factors, such as pH, temperature, oxygen, light, enzymes, nucleophilic agents, sugar derivatives and co-pigments, have affected on anthocyanin stability. For this reason, the extraction method should be thoroughly checked for the qualitative/quantitative analysis of anthocyanin in particular plant material. To identify the optimum extraction method of cyanidin-3-glucoside, major anthocyanin of dark purple-colored grains, Oryza sativa cv. Heugjinjubyeo, Phaselous vulgaris, Phynchosia gngularis, Sesamum indium, Rhynchosia nulubilis and Lablab purpureus, reversed-phase HPLC analysis using solvent system of acetonitrile, methanol and water were accomplished.

• 생약학회지
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• 제30권2호
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• pp.158-162
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• 1999

The rhizomes of Salvia miltiorrhiza Bunge(Labiatae) has been used in Chinese traditional medicine for the treatment of coronary heart diseases and myocardial infarction. As a part of a research for standardization of crude oriental drugs, we have determined the content of tanshinone IIA in Salvia miltiorrhiza purchased from various regions of Korea. The HPLC method by which quantitative analysis was conducted, showed reproducible results and chromatographic isolation of tanshinone IIA was accomplished successively.

• International Journal of Safety
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• 제6권2호
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• pp.8-12
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• 2007

This study includes a case study among plastic process manufacturing companies, based on which, the currently used 4M method is applied in terms of machine, media, man, and management, to conduct quantitative risk evaluation, and thus to contribute to reducing human and material loss as well as preventing accidents in industrial fields. The result of this study is analyzed based on the 4M-risk assessment to find out the hazardous risk elements, and the quantitative evaluation made it predictable the value of risk(frequency $\times$ intensity) in such classified levels as serious risk, critical risk, and intolerable risk. Further, Among the businesses with hazardous risk elements and high frequency of industrial disaster, risk analysis was conducted for each process, and as a result, 38 cases among 76, including those of serious risk, critical risk, and intolerable risk, were improved, and the risk was reduced. Besides, it is thought that with the engineering approach with 4M-Risk Assessment, the attempt to improve safety level contributes to prevention of accidents.