• Journal of Ginseng Research
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• v.41 no.3
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• pp.403-410
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• 2017

Background: Prenyltransferases catalyze the sequential addition of isopentenyl diphosphate units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cis-prenyltransferases (CPTs). The functions of CPTs have been well characterized in bacteria, yeast, and mammals compared to plants. The characterization of CPTs also has been less studied than TPTs. In the present study, molecular cloning and functional characterization of a CPT from a medicinal plant, Panax ginseng Mayer were addressed. Methods: Gene expression patterns of PgCPT1 were analyzed by quantitative reverse transcription polymerase chain reaction. In planta transformation was generated by floral dipping using Agrobacterium tumefaciens. Yeast transformation was performed by lithium acetate and heat-shock for $rer2{\Delta}$ complementation and yeast-two-hybrid assay. Results: The ginseng genome contains at least one family of three putative CPT genes. PgCPT1 is expressed in all organs, but more predominantly in the leaves. Overexpression of PgCPT1 did not show any plant growth defect, and its protein can complement yeast mutant $rer2{\Delta}$ via possible protein-protein interaction with PgCPTL2. Conclusion: Partial complementation of the yeast dolichol biosynthesis mutant $rer2{\Delta}$ suggested that PgCPT1 is involved in dolichol biosynthesis. Direct protein interaction between PgCPT1 and a human Nogo-B receptor homolog suggests that PgCPT1 requires an accessory component for proper function.

• Journal of Korea Water Resources Association
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• v.47 no.1
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• pp.49-62
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• 2014

As the Urban area grows and more land is developed both within the city and in surrounding areas, hydrologic functions of the natural water cycle are altered. Urbanization creates impervious areas that negatively impact stormwater runoff characteristics. these changes to the natural hydrologic cycle result in the increased flooding, decreased groundwater recharge, increased urban heat island effects. Finally, the land use and other activities result in accumulation and washoff of pollutants from surface, resulting in water quality degradation. Therefore, in this study, evaluating and quantitative analysis of the percolation effect through infiltration experiment of permeable pavement, which is one of the ways that can reduce the problem of the dry stream. Also the SWMM model is used to study the effect of the hydrologic cycle for permeable pavement block contribution.

• Molecules and Cells
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• v.21 no.2
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• pp.261-268
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• 2006

The Drosophila methuselah (mth) mutant has an approximately 35 percent increase in average lifespan, and enhanced resistance to various forms of stress, including starvation, high temperature, and dietary paraquat. To examine the transcriptional regulation of mth, we used luciferase assays employing Drosophila S2 cells. Two positive control elements were found at -542 ~ -272 (PE1) and +28 ~ +217 (PE2), where putative binding sites for transcription factors including Dorsal (Dl) were identified. Cotransfection of a Dl expression plasmid with a mth-luciferase reporter plasmid resulted in decreased reporter activity. PE1 and PE2, the minimal elements for strong promoter activity, were required for maximal repression by Dl protein. The N-terminal Rel homology domain (RHD) of Dl was not sufficient for repression of mth. We demonstrated by chromatin affinity precipitation (ChAP) assays in S2 cells that Dl bound to the putative PE1 binding site. Unexpectedly, semi-quantitative RT-PCR analysis revealed that the level of mth transcripts was reduced in dl flies. However, the in vivo result support the view that mth expression is regulated by dl, since it is well known that Dl functions as both a transcriptional activator and repressor depending on what other transcription factors are present. These findings suggest that both innate immunity and resistance to stress are controlled by Dl protein.

• The Transactions of the Korea Information Processing Society
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• v.4 no.7
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• pp.1719-1732
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• 1997

In the software development process, various development product are made in each phase of lifecycle. Formless software is visualized by products, and quality management is implemented by management for products. But, because standardization for products which is made in the development process is not established practically, developers use different development methodologies or documentation specifications. Therefore the reality is that quality evaluation is not implemented along standardized methodology or systematic process. In this paper, we constructed quality management system and designed and implemented analysis phase quality evaluation toolkit. We constructed quality external and internal character to evaluate software quality for quality management and constructed relational metrics and system of qualitative quantitative evaluation element items and, under these systems, we constructed quality evaluation toolkit and Implemented functions and evaluated products.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.4
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• pp.299-307
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• 2015

Severe acute pancreatitis (SAP) is normally related to multiorgan dysfunction and local complications. Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP. The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction. Rats in the sham group were injected with normal saline in identical locations. We also investigated the regulation of experimentally induced SAP on local RAS expression in the pancreas through determination of the activities of serum amylase, lipase and myeloperoxidase, histological and biochemical analysis, radioimmunoassay, fluorescence quantitative PCR and Western blot analysis. The results indicated that valsartan could effectively suppress the local RAS to protect against experimental acute pancreatitis through inhibition of microcirculation disturbances and inflammation. The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists. Moreover, other RAS inhibitors could be a new therapeutic target in acute pancreatitis.

• Journal of the Korean Society of Safety
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• v.18 no.3
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• pp.88-93
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• 2003

The Process Safety Management (PSM) by the Law of Industry, Safety and Health has been performed for preventing major accidents of chemical plants since 1996. In terms of preventing chemical accidents more precisely, it is essential to develop a tool for quantitative risk assessment. For this, KOSHA (Korea Occupational Safety and Health Agency) developed an Integrated Risk Management System (IRMS). The system is designed to assimilate data on chemical plant hazards from external database, to integrate these data with location information(topographic and demographic), and to make them user-friendly accessible. The system consists of several main functions: display of five major Korean petrochemical complex layout display of equipment layout with its information utilizing the external database, zonation of the hazard effected area with consequence analyses, the most probable accident scenario generation, accident/incident database and calculation of frequency of accident using equipment reliability database, etc. The highlight of IRMS is to provide the risk contours using GIS(Geographical Information System) technology. IRMS is intended to manage hazardous installation more systematically and effectively, to reduce the number of accident remarkably, further minimizing production loss in the plant. The system is now under application to about 500 PSM sites as well as and emergency authorities in Korea by KOSHA (Korea Occupational Safety and Health Agency)

• Journal of the Korean Institute of Landscape Architecture
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• v.26 no.3
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• pp.78-78
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• 1998

The purpose of this research is to develop a CAD-based program for data analysis of digital elevation model(DEM) on the aspect of landscape assessment. When handling DEM data as a visual simulation of topographic landscape, it is basic interest to analyze visible area and visualize visual sensitivity distributions. In reference with landscape assessment, more intuitive and interactive visualizing tools are needed, specially in area of visual approach. For adaptability to landscape assessment, algorithmic approaches to visibility analysis and concepts for visual sensitivity calculation in this study were based on processing techniques of entity data control functions used in AutoCAD drawing database. Also, for the purpose of quantitative analysis, grid-type 3DFACE entities were adopted as mesh unit of DEM structure. Developed programs are composed of main part named VSI written in AutoLISP and two of interface modules written in dialog control language(DCL0 for user-oriented interactive usage. Definitions of camera points(view points) and target points(or observed area) are available alternatively in combined methods of representing scenic landscape, scenery, and sequential landscape. In the case of scene landscape(single camera to fixed target point), only visibility analysis in available. And total visibility, frequency of cumulative visibility, and visual sensitivity analysis are available in other cases. Visual sensitivity was thought as view angle(3 dimensional observed visual area) and the strengths were classified in user defined level referring to statistical characteristics of distribution. Visibility analysis routine of the VSI was proved to be more effective in the accuracy and time comparing with similar modules of existing AutoCAD third utility.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.7 no.2
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• pp.53-61
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• 1989

In this paper the quantitative classification parameters of terrain which can be practicable to the interpolation of digital terrain model forming a regular grid pattern have been suggested and objective terrain classification have been established by making a cluster analysis using these parameters. Also, interpolation suitable to the classification of terrain has been used by making a descriminant alaysis from description parameters of terrains. The terrain classification in this paper was dependent upon two parameters of the ratio horizontal area to inclined area and the magnitude of harmonic vectors. And the studying area was seperated to three groups of terrains by these two parameters. Three groups of terrains could be classified into the discriminant functions. By determining the ratio of area and harmonic vector magnitude in any terrains using the above discriminant function, it was possible to discriminate the terrains to apply the interpolation practicable to the terrain characteristics.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.5
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• pp.3107-3113
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• 2014

We present a quantitative analysis of a dipole antenna and its characteristics from the viewpoint of quantum mechanics. The method makes use of a Maxwell equation used in an existing antenna propagation formula. This includes radiation resistance, input reactance, and antenna efficiency as functions of frequency and antenna length. Particular attention is paid to the Schr$\ddot{o}$odinger equation. We accomplish E-field and H-field analyses of a dipole antenna by combining the Maxwell and Schr$\ddot{o}$odinger wave equations. When comparing the existing Maxwell wave equation with the Schr$\ddot{o}$odinger wave equation, quantum-electric movement is more accurate than using the Maxwell wave equation alone.

• Journal of Ocean Engineering and Technology
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• v.30 no.5
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• pp.349-355
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• 2016

Divinycell, which functions as both insulation and a supporting structure, is generally applied in the NO96-type liquefied natural gas (LNG) insulation system. Polymer-material-based Divinycell, which has a high strength and low weight, has been widely used in the offshore, transportation, wind power generation, and civil engineering fields. In particular, this type of material receives attention as an insulation material because its thermal conductivity can be lowered depending on the ambient temperature. However, it is difficult to obtain research results for Divinycell, even though the component materials of the NO96-type LNG cargo containment system, such as 36% nickel steel (invar steel), plywood, perlite, and glass wool, have been extensively studied and reported. In the present study, temperature and strain-rate dependent compressive tests on Divinycell were performed. Both the quantitative experimental data and elastic recovery are discussed. Finally, the mechanical characteristics of Divinycell were compared to the results of polyurethane foam insulation material.