• 제목/요약/키워드: Quality control mechanism

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Construction of a High-Quality Yeast Two-Hybrid Library and Its Application in Identification of Interacting Proteins with Brn1 in Curvularia lunata

  • Gao, Jin-Xin;Jing, Jing;Yu, Chuan-Jin;Chen, Jie
    • The Plant Pathology Journal
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    • v.31 no.2
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    • pp.108-114
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    • 2015
  • Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated. An yeast two-hybrid (Y2H) library is a very useful platform to further unravel novel pathogenic factors in C. lunata. To construct a high-quality full length-expression cDNA library from the C. lunata for application to pathogenesis-related protein-protein interaction screening, total RNA was extracted. The SMART (Switching Mechanism At 5' end of the RNA Transcript) technique was used for cDNA synthesis. Double-stranded cDNA was ligated into the pGADT7-Rec vector with Herring Testes Carrier DNA using homologous recombination method. The ligation mixture was transformed into competent yeast AH109 cells to construct the primary cDNA library. Eventually, a high qualitative library was successfully established according to an evaluation on quality. The transformation efficiency was about $6.39{\times}10^5$ transformants/$3{\mu}g$ pGADT7-Rec. The titer of the primary cDNA library was $2.5{\times}10^8cfu/mL$. The numbers for the cDNA library was $2.46{\times}10^5$. Randomly picked clones show that the recombination rate was 88.24%. Gel electrophoresis results indicated that the fragments ranged from 0.4 kb to 3.0 kb. Melanin synthesis protein Brn1 (1,3,8-hydroxynaphthalene reductase) was used as a "bait" to test the sufficiency of the Y2H library. As a result, a cDNA clone encoding VelB protein that was known to be involved in the regulation of diverse cellular processes, including control of secondary metabolism containing melanin and toxin production in many filamentous fungi was identified. Further study on the exact role of the VelB gene is underway.

QoS Aware Cross-layer MAC Protocol in wireless Sensor Networks (무선 센서 네트워크에서 QoS를 인지하는 Cross-layer MAC 프로토콜)

  • Park, Hyun-Joo;Kim, Seong-Cheol
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.12
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    • pp.2811-2817
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    • 2010
  • In this paper we propose the QAC-MAC that supports Quality of Service(QoS) and saves energy resources of the sensor node, and hence prolonging the lifetime of the sensor network with multiple sink nodes. Generally, the nodes nearest to the sink node often experience heavy congestion since all data is forwarded toward the sink through those nodes. So this critically effects on the delay-constraint data traffics. QAC-MAC uses a hybrid mechanism that adapts scheduled scheme for medium access and scheduling and unscheduled scheme based on TDMA for no data collision transmission. Generally speaking, characteristics of the real-time traffic with higher priority tends to be bursty and has same destination. QAC-MAC adapts cross-layer concept to rearrange the data transmission order in each sensor node's queue, saves energy consumption by allowing few nodes in data transmission, and prolongs the network lifetime.

Simulating Arsenic Concentration Changes in Small Agricultrual Reservoir Using EFDC-WASP Linkage Model (EFDC-WASP 연계모형을 이용한 소규모 농업용 저수지 비소 농도 모의)

  • Hwang, Soonho;Shin, Sat Byeol;Song, Jung-Hun;Yoon, Kwang Sik;Kang, Moon Seong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.60 no.5
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    • pp.29-40
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    • 2018
  • Even if a small amount of arsenic (As) is entering to small agricultural reservoir from upper streams, small agricultural reservoir becomes sensitive to changes in arsenic concentration depending on the water level in case of accumulation continuously because of its scale. If we want to manage arsenic concentration in small agricultural reservoir, it is very important to understand arsenic changes in agricultural reservoir. In spite of the fact that modeling is the most accurate method for analyzing arsenic concentration changes in small agricultural reservoirs, but, it is difficult to monitor arsenic change everyday. So, if data is prepared for modeling arsenic changes, water quality modeling is more effective than monitoring. Therefore, in this study, arsenic concentration changes was simulated and arsenic concentration change mechanism in small reservoir was analyzed using hydrological and water quality monitoring data and by conducting EFDC (Environment Fluid Dynamics Code)-WASP (Water Quality Analysis Simulation Program) linkage. EFDC-WASP coupling technique was very useful for modeling arsenic changes because EFDC can consider hydrodynamic and WASP can perform arsenic concentration simulation, separately. As a results of this study, during dry season, As concentration was maintained relatively high arsenic concentrations. Therefore, water level control will be needed for managing As concentration of reservoir.

The Effect of Compressed Video Traffic over ABR on Satellite ATM Networks (위성 ATM 망에서 압축된 비디오 트래픽의 ABR 서비스에 미치는 영향)

  • 김성철;이상은
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.24 no.9A
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    • pp.1285-1294
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    • 1999
  • In this paper we consider the performance of TCP video traffic over ABR with Long-Range Dependent VBR traffic. As compressed coded video traffics are increasing rapidly over Internet, lots of studies are being done for transmitting those traffics efficiently using limited network resources. We consider here the transmitting video service over ABR service in ATM networks, especially satellite networks. CBR or VBR services are suggested in transmitting the video traffic in ATM Forum TM 4.0. But ABR service connection, which is considered as appropriate service for data traffic, can be established with a small amount of bandwidth, MCR (Minimum cell rate). Furthermore ABR service can control the source's transmitting rate using feedback mechanism. Using this feature ABR service can be used in some applications which can control their quality of services corresponding to network loads. Compressed video sources with MPEG-2 are used for Long-Range Dependent VBR traffic here. We model the compressed video source to resemble the MPEG-2 transport streams. These compressed video traffic streams are consisted of three different frames, I-frame, P-frame, and B-frame. So when a network are overloaded, we can control the quality of service using this traffic features. TCP Traffics over ABR need large buffers in ATM switch to satisfy their QoS with background VBR traffics, which have high deviations in bandwidth. Furthermore satellite ATM networks with large feedback delay need large buffers corresponding RTT delay. The performance comparisons among EFCI and ER switch (ERICA+) switches in the network circumstances described above were shown in this paper. We also considered the case with ON-OFF VBR traffics.

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Design of Vision Based Punching Machine having Serial Communication

  • Lee, Young-Choon;Lee, Seong-Cheol;Kim, Seong-Min
    • 제어로봇시스템학회:학술대회논문집
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    • 2005.06a
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    • pp.2430-2434
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    • 2005
  • Automatic FPC punching instrument for the improvement of working condition and cost saving is introduced in this paper. FPC(flexible printed circuit) is used to detect the contact position of K/B and button like a cellular phone. Depending on the quality of the printed ink and position of reference punching point to the FPC, the resistance and current are varied to the malfunctioning values. The size of reference punching point is 2mm and the above. Because the punching operation is done manually, the accuracy of the punching degree is varied with operator's condition. Recently, The punching accuracy has deteriorated severely to the 2mm punching reference hall so that assembly of the K/B has hardly done. To improve this manual punching operation to the FPC, automatic FPC punching system is introduced. Precise mechanical parts like a 5-step stepping motor and ball screw mechanism are designed and tested and low cost PC camera is used for the sake of cost down instead of using high quality vision systems for the FA. 3D Mechanical design tool(Pro/E) is used to manage the exact tolerance circumstances and avoid design failures. Simulation is performed to make the complete vision based punching machine before assembly, and this procedure led to the manufacturing cost saving. As the image processing algorithms, dilation, erosion, and threshold calculation is applied to obtain an exact center position from the FPC print marks. These image processing algorithms made the original images having various noises have clean binary pixels which is easy to calculate the center position of print marks. Moment and Least square method are used to calculate the center position of objects. In this development circumstance, Moment method was superior to the Least square one at the calculation of speed and against noise. Main control panel is programmed by Visual C++ and graphical Active X for the whole management of vision based automatic punching machine. Operating modes like manual, calibration, and automatic mode are added to the main control panel for the compensation of bad FPC print conditions and mechanical tolerance occurring in the case of punch and die reassembly. Test algorithms and programs showed good results to the designed automatic punching system and led to the increase of productivity and huge cost down to law material like FPC by avoiding bad quality.

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Characteristics of Korean Trauma Patients: A Single-center Analysis Using the Korea Trauma Database

  • Park, Youngeun;Chung, Min;Lee, Gil Jae;Lee, Min A;Park, Jae Jeong;Choi, Kang Kook;Hyun, Sung Youl;Jeon, Yang Bin;Ma, Dae Sung;Yoon, Yong-Cheol;Lee, Jungnam;Yoo, Byungchul
    • Journal of Trauma and Injury
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    • v.29 no.4
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    • pp.155-160
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    • 2016
  • Purpose: Two years have passed since a level I trauma center was officially opened in the Gacheon Gil Hospital, South Korea. We analyzed 2014 and 2015 registered patient data from the Korean Trauma Data Base (KTDB) to identify trends in trauma patient care and factors that influence the quality of trauma care at the Gacheon Gil trauma center. Methods: Data was extracted from the KTDB included patient age, sex, systolic blood pressure at emergency room arrival, revised trauma score, injury severity score, trauma injury severity score, transfusion amount, and the cause of death was analyzed. Results: A total of 3269 trauma patients were admitted to our trauma center in 2014 and 3225 in 2015. Demographics and mechanism of injury were not significantly different between years. The severity of trauma injury was decreased in 2015 although the mortality rate was slightly increased. This requires further analysis. Conclusion: The aim of this study was to determine the general status and trends in trauma incidence and management outcomes for the Incheon area. We noted no significant changes in trauma status from 2014 to 2015. We need to collect and review trauma patient data over a long period in order to elucidate trauma incidence and management trends in the trauma field. Finally, studies using trauma patient data will indicate appropriate quality control factors for trauma care and help to improve the quality of trauma management.

The Design of th GRACE-LB Algorithm for Congestion Control in Broadband ISDN ATM Network (광대역 ISDN ATM 네트워크의 과잉 밀집 제어를 위한 GRACE-LB 알고리즘의 설계)

  • 곽귀일;송주석
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.18 no.5
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    • pp.708-720
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    • 1993
  • The new preventive control mechanisms for traffic management in BISDN/ATM networks can be divided into Connection Admission Control(CAC), Usage Parameter Control (UPC), and Priority Control. Of these mechanism, Usage Parameter Control continuously monitors the parameters admitted in the network's entry point to guarantee quality of service of connections already admitted. Upon detecting traffic that violates the negotiated parameter, it takes the necessary control measures to prevent congestion. Among these traffic control methods, this paper focuses on the Usage Parameter Control method, and proposes and designs GRACE-LB(Guaranteed Rate Acceptance & Control Element-using Leaky Bucket) which improves upon existing UPC models. GRACE-LB modifies the previous LB model by eliminating the cell buffer, dividing the token Pool into two pools, Long-term pool, Short-term pool, and changing the long-term token generating form using 'Cycle Token' into the same bursty form as the traffic source. Through this, GRACE-LB achieves effective control of the Average Bit Rate(ABR) and burst duration of bursty multimedia traffic which previous LB models found difficult to control. Also, since GRACE-LB can e implemented using only simple operations and there are no cell buffers in it, it has the merit of being easily installed at any place.

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A Study on the Development Strategies of the Priority Job Training Program (우선선정직종훈련제도의 발전방안 연구)

  • Yoo, Kil-Sang
    • The Journal of Korean Institute for Practical Engineering Education
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    • v.2 no.1
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    • pp.120-125
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    • 2010
  • The priority job training program is one of key training programs in Korea. This training program has some fundamental problems. The process of selecting the detailed training jobs does not reveal the true training demand of industry sectors. The contracting-out process and control mechanism of the priority job training program show several weaknesses from the points of success conditions of contracting-out public services. In this paper, I proposed development strategies of the priority job training program. The contract period should be longer than one year so that the training institutions may invest for improving quality of training in the longer perspective and reduce transaction costs. The training fee pay mechanism should be more performance-based.

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Development of a Multi-material Stereolithography System (다중재료 광조형장치 개발)

  • Kim, Ho-Chan;Choi, Jae-Won;Wicker, Ryan
    • Journal of the Korean Society for Precision Engineering
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    • v.27 no.3
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    • pp.135-141
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    • 2010
  • Researchers continue to explore possibilities for expanding additive manufacturing (AM) technologies into direct product manufacturing. One limitation is in the materials available for use in AM that can meet the needs of end-use applications. Stereolithography (SL) is an AM technology well known for its precision and high quality surface finish capabilities. SL builds parts by selectively crosslinking or solidifying photo-curable liquid resins, and the resin industry has been continuously developing new resins with improved performance characteristics. This paper introduces a unique SL machine that can fabricate parts out of multiple SL materials. The technology is based on using multiple vats positioned on a rotating vat carousel that contain different photo-curable materials. To change the material during the process, the build platform is raised out of the current vat, a new vat with a different material is rotated under the platform, and the platform is submerged into the new vat so that the new material can be used. This paper introduces a new vat exchange mechanism, cleaning process, recoating process, resin leveling mechanism and process planning technologies for the implementation of multiple material SL. An overview of the system framework is provided and the system integration and control software is described. In addition, several multiple material test parts are designed, fabricated, and described.

Study on Magnetic Property for Test Coil and Permanent Magnet (Test Coil과 영구자석의 자기 특성 연구)

  • Park, Yun Bum;Kim, Jong Wook;Lee, Jae Seon
    • Journal of the Korean Magnetics Society
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    • v.26 no.5
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    • pp.154-158
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    • 2016
  • A CRDM (Control Rod Drive Mechanism) is an electromagnetic device which drives a control rod assembly linearly to regulate the reactivity of a nuclear core. An RPIS (Rod Position Indication System) is used as a position indicator for a control rod assembly of a CRDM of SMART, and an RPIS consists of permanent magnets and reed switches. SMART is designed for the maximum coolant temperature of $350^{\circ}C$, and the permanent magnets are installed inside of the reactor. The reed switches and electrical circuit are installed outside of the reactor on the other hand. Test coil for a reed switch is test equipment for quality verification of a reed switch, and a test coil consists of a coil and core. In this study, magnetic property of test coil and permanent magnet on a reed switch is compared by using finite element electromagnetic simulation.