• Animal Bioscience
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• 제35권2호
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• pp.166-176
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• 2022

Objective: Sperm is particularly susceptible to reactive oxygen species (ROS) stress. Glutathione (GSH) is an endogenous antioxidant that regulates sperm redox homeostasis. However, it is not clear whether boar sperm could utilize cysteine for synthesis GSH to protect sperm quality from ROS damage. Therefore, the present study was undertaken to elucidate the mechanism of how cysteine is involved in protecting boar sperm quality during liquid storage. Methods: Sperm motility, membrane integrity, lipid peroxidation, 4-hydroxyIlonenal (4-HNE) modifications, mitochondrial membrane potential, as well as the levels of ROS, GSH, and, ATP were evaluated. Moreover, the enzymes (GCLC: glutamate cysteine ligase; GSS: glutathione synthetase) that are involved in glutathione synthesis from cysteine precursor were detected by western blotting. Results: Compared to the control, addition of 1.25 mM cysteine to the liquid storage significantly increased boar sperm progressive motility, straight-line velocity, curvilinear velocity, beat-cross frequency, membrane integrity, mitochondrial membrane potential, ATP level, acrosome integrity, activities of superoxide dismutase and catalase, and GSH level, while reducing the ROS level, lipid peroxidation and 4-HNE modifications. It was also observed that the GCLC and GSS were expressed in boar sperm. Interestingly, when we used menadione to induce sperm with ROS stress, the menadione associated damages were observed to be reduced by the cysteine supplementation. Moreover, compared to the cysteine treatment, the γ-glutamylcysteine synthetase (γ-GCS) activity, GSH level, mitochondrial membrane potential, ATP level, membrane integrity and progressive motility in boar sperm were decreased by supplementing with an inhibitor of GSH synthesis, buthionine sulfoximine. Conclusion: These data suggest that boar sperm could biosynthesize the GSH from cysteine in vitro. Therefore, during storage, addition of cysteine improves boar sperm quality via enhancing the GSH synthesis to resist ROS stress.

• 한국환경보건학회지
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• 제43권4호
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• pp.334-348
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• 2017

Objectives: We investigated the impacts of indoor plants on indoor air quality and occupational health, focusing on allergic rhinconjunctivitis and stress among employees in new office buildings. Methods: A total of 34 employees working at new public office buildings were enrolled as subjects (n=17, with indoor plants) and as a control (n=17) group. Before and after introducing indoor plants for three months, indoor air quality measurements including VOCs and aldehydes and questionnaires on sick building syndrome, AR symptoms (ARIA based), stress (DASS 42, KOSS, and SACL), and indoor characteristics were performed and statistically analysed. Results: Among the 34 enrolled subjects, 19 were included in the probable AR subject group (subjects with indoor plants, n=8, control n=11) and completed all questionnaires. Statistical analyses were done for total, AR subject groups, and controls. As a result, it was confirmed that major indoor air pollutants decreased after the introduction of indoor plants (p<0.5). Among major symptoms of allergic rhinoconjunctivitis, watery rhinorrhea, nasal stuffiness, and nasal itching indexes decreased (p<0.5, respectively). A decrease was noted in some areas of work-related stress indexes (mainly KOSS) among the subject group (total and AR) and a decrease of indoor environmental attractiveness among the control group (total and AR) (p<0.5, for all). Conclusions: Indoor plants may help reduce indoor air pollutants and decrease AR symptoms and work-related stress of employees in newly built office buildings. Various further follow-up studies on the mechanism of environmental, physical, and emotional influences and utilization of indoor plants in association with allergic diseases will be needed.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권8호
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• pp.3621-3640
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• 2016

In the process of k-coverage of the target node, there will be a lot of data redundancy forcing the phenomenon of congestion which reduces network communication capability and coverage, and accelerates network energy consumption. Therefore, this paper proposes a novel energy balanced k-coverage control algorithm based on probability model (EBKCCA). The algorithm constructs the coverage network model by using the positional relationship between the nodes. By analyzing the network model, the coverage expected value of nodes and the minimum number of nodes in the monitoring area are given. In terms of energy consumption, this paper gives the proportion of energy conversion functions between working nodes and neighboring nodes. By using the function proportional to schedule low energy nodes, we achieve the energy balance of the whole network and optimizing network resources. The last simulation experiments indicate that this algorithm can not only improve the quality of network coverage, but also completely inhibit the rapid energy consumption of node, and extend the network lifetime.

• 한국정밀공학회지
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• 제33권6호
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• pp.453-458
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• 2016

CFRP (Carbon Fiber Reinforced Plastic) and CFRP-metal stacks have recently been widely used in the aerospace and automobile industries. When CFRP is machined by a brittle fracture mechanism, defect generation behaviors are different from those associated with metal cutting. The machining quality is strongly dependent on the properties of CFRP materials. Therefore, process control for CFRP machining is necessary to minimize the defects of differently manufactured CFRPs. In this study, defects in drilling of CFRP substrates with a variety of fiber directions and resin types are compared with respect to thrust force. An experimental study on material interface detection is carried out to investigate its benefits in process control.

• 대한기계학회논문집
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• 제17권8호
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• pp.1951-1962
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• 1993

The creation process of a typical machined surface is treated here as a dynamic system. An investigation is carried out to establish a relationship between the characteristics of cutting force fluctuations that cause vibration response of the tool-workpiece system and the formation of surface in face cutting by sintered carbide cutting tool. Cutting force is measured and analyzed in frequency domain. The power spectral densities of cutting force give a useful information in surface generation and it can be used to find out the control factor of surface roughness. The terms, PSD ratio & Normalized spindle frequency PSD, are defined and when the value of power in spindle frequency is absolutely little but relatively large, it is obtained high accuracy surface roughness. The aim of this research is to find surface profile by measured and analyzed cutting force signals. The simulation of surface generation gives the comprechension of its mechanism and help to predict and control the surface quality. In this study, it is suggested what informations about surface generation can be acquired from the cuttuing force signal and an way of generating a better surface.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2007년도 학술발표회 논문집
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• pp.454-458
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• 2007

Nonpoint source (NPS) pollution is a serious problem causing the degradation of soil and water quality. Concentrated overland flow is the primary transport mechanism for a large amount of NPS pollutants from hillslope areas to downslope areas in a watershed. In this study, a soil erosion model, nLS model, to identify transitional overland flow regions (i.e., ephemeral gully head areas) was developed using the kinematic wave overland flow theory. Spatial data, including digital elevation models (DEMs), soil, and landcover, were used in the GIS-based model algorithm. The model was calibrated and validated using gully head locations in a large agricultural watershed, which were identified using 1-m aerial photography. The model performance was better than two previous approaches; the overall accuracy of the nLS model was 72 % to 87 % in one calibration subwatershed and the mean overall accuracy was 75 to 89 % in four validation subwatersheds, showing that the model well predicted potential transitional erosion areas at different watersheds. However, the user accuracy in calibration and validation was still low. To improve the user accuracy and study the effects of DEM resolution, finer resolution DEMs may be preferred because DEM grid is strongly sensitive to estimating model parameters. Information gained from this study can improve assessing soil erosion process due to concentrated overland flow as well as analyze the effect of microtopographic landscapes, such as riparian buffer areas, in NPS control.

• 한국철도학회논문집
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• 제2권4호
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• pp.9-19
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• 1999

The use of compaction grouting evolved in 1950's to correct structural settlement of buildings. Over the almost 50 years, the technology has been developed and is currently used in wide range of applications. Compaction grouting, the injection of a very stiff, 'zero-slump' mortar grout under relatively high pressure, displaces and compacts soils. It can effectively repair natural or man-made soil strength deficiencies in variety of soil formations. Major applications of compaction grouting include densifying loose soils or fill voids caused by sinkholes, poorly compacted fills, broken utilities, improper dewatering, or soft ground tunneling excavation. Other applications include preventing liquefaction, re-leveling settled structures, and using compaction grout bulbs as structural elements of minipiles or underpinning. In this paper, on the basis of the case history constructed in this year, a study has been performed to analyze the basic mechanism of the compaction grouting. Also, the effectiveness of the ground improvement and the bearing capacity of the compaction pile has been verified by the Cone Penetration Test(CPT) and Load Test. Relatively uniform compaction grouting column could be maintained by planning the quality control in the course of grouting. And, the Qualify Control Plan has been conceived using grout pressure, volume of grout and drilling depth.

• 제어로봇시스템학회논문지
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• 제13권12호
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• pp.1252-1256
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• 2007

U-learning (ubiquitous learning) systems, which deliver learning materials anytime and anywhere, allow learners to watch live lectures on PDAs, tablet PCs and notebook computers via broadband and wireless Internet. These systems have various problems; first, terminal devices are expensive, and it is difficult to maintain their efficiencies. Secondly, Internet does not guarantee quality of service (QoS), and in general it does not provide real-time services. Finally, the security of these systems is weaker in a local network than in an external network. The USB-based terminal system based on the OSGi service platform was designed as a ubiquitous system, in order to solve those problems. The USB terminals, used in this system, are inexpensive, and it is easy to maintain their performances. Also, this system solves the problems of security in a local network and provides guaranteed QoS. To accomplish this, the number of USB terminals connected to the system has to be limited according to the formula proposed in our paper. This system uses the OSGi specification as a middleware. It supports the discovery mechanism of the USB terminals, maintenance and administration of the system. Finally, this paper shows a driver's license testing system as an example u-learning application1.

• 한국콘텐츠학회논문지
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• 제10권3호
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• pp.80-91
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• 2010

방송 통신의 융합에 따라 차세대 멀티미디어 서비스인 IPTV 서비스가 각광받고 있다. IPTV 서비스는 무한한 채널수용과 미디어의 확장, 품질이 보장되는 서비스의 형태로 발전되면서 기존 네트워크의 트래픽 증가와 서비스 특성에 맞는 품질제어 기술들이 요구되고 이에 따라 플로우별 서비스 품질 제어 기술이 필요하게 되었다. 이를 위해 IETF (Internet Engineering Task Force)에서도 인터넷 상에서 IP QoS를 제공하기 위한 연구가 활발히 이루어지고 있는데 그 중 버퍼 관리 메커니즘으로 RED(Random Early Detection) 알고리즘 및 RED를 변형한 여러 가지 방법들이 제시되고 있으나 다양한 멀티미디어 서비스의 QoS 요구 사항을 충족시키는데 어려움이 있다. 이에 따라, 본 논문에서는 다양한 QoS를 요구하는 인터넷 멀티미디어 서비스를 지원하기 위해 각 서비스가 요구하는 목표 패킷 손실률을 유지시킬 수 있는 QoS적응형 플로우 기반 AQM (Active Queue Management) 알고리즘을 제안하고 ns-2를 이용한 시뮬레이션을 통해 성능 분석을 제시하였다.

• 생명과학회지
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• 제17권6호통권86호
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• pp.847-858
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• 2007

ER-Golgi 분비 경로를 통해서 정확한 구조를 가지면서 post-translational modification 과정을 거친 재조합 단백질의 발현을 최대화하는 것은 ER stress반응에 대한 연구의 중요한 계기가 된다. 세포가 스트레스를 받지 않는 상태라도 ER stress signaling은 재조합 단백질의 생산량을 제한하고 품질을 떨어뜨리는 여러 가지 조건을 만들게 된다. ER stress signaling을 막는 여러 가지 방법들이 제시되고 있으며 표 2는 이러한 방법들 중 일부를 나타내고 있다. 일반적으로는 pro-survival 경로에 관련되어 있는 인자를 촉진하고 pro-apoptosis에 관련되어 있는 인자를 억제하는 것들이다. 그러나 ER stress 반응은 매우 복잡하고 적응과 사멸 기작(adaptation and elimination mechanism)의 중간 역할을 하기 때문에 ER stress에 관련된 주요 인자를 산업적으로 응용하기 위해선 이들의 기능에 대해 보다 깊은 연구가 이루어져야 한다. 현재까지 재조합단백질의 생산량을 최대한으로 높이는 방법은 ER stress 반응이 생기지 않도록 fed-batch process를 개선하고 세포 사멸 기작을 조절하며 단백질의 glycosylation 처리를 하는 것이다.