• Biomedical Science Letters
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• v.22 no.3
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• pp.83-97
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• 2016

The measurement of viral load in HIV-1 infected patients is essential for the establishment of a therapeutic strategy. Several commercial assays have shown shortcomings in quantifying rare genotypes of HIV-1 such as minor groups of N and O. In this study, the HIV-1 RT-qPCR assay was developed. The primers and probe of HIV-1 were designed to target the pol gene and to increase the detection efficiency of various subtypes including group N and O. The HIV-1 quantitative RT-qPCR assay was assessed for its analytical performance and clinical evaluation. The LoD was determined to 33.9 IU/ml. The LoD of several subtypes including A, C, D, CRF_01AE, F, CRF_02AG, G and H, were determined to less than 40 IU/ml. The HIV-1 quantitative RT-qPCR assay was evaluated using the China National Reference Panel of HIV-1 RNA to determine the analytical performance. The results were all within the acceptable range. The clinical evaluation was performed at Hunan CDC in China. The clinical evaluation results were compared with those of the China domestic commercial kit. A significant correlation (fresh samples; $R^2=0.84$, P<0.001, frozen samples; $R^2=0.76$, P<0.001) between the two systems was observed for 64 fresh samples and 76 frozen samples with viral loads, and the Bland-Altman plot showed good agreement (98.4%, 96.1%, respectively). In conclusion, the HIV-1 quantitative RT-qPCR assay had comparable analytical performance with several commercial kits. The study provides basic data for the research of HIV-1 diagnosis and the development of P < HIV-1 molecular diagnostic assay.

• Korean Journal of Environment and Ecology
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• v.17 no.4
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• pp.316-323
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• 2004

In order to monitor the vegetation restoration in the evergreen broad-leaved forest, eighteen experimental plots including control plots were established at Wando Arboretum in 1996. Several treatments such as density control, selective cutting and warm temperate species planting were done in the experimental plots. Seven years after the restoration experiments, the important percentage of Quercus acuta showed a tendency to a higher increase in the experiment plots than control plots in Q. Acutu. Also the important percentage of Q. Acuta in Q. acute Carpinus tschonoskii community increased in the tree layer. Pinus densiflora community was increased highly in important percentage of Q. Acuta, As a whole, vegetation structure in the experiment plot showed successional stage to Q. Acutu community. In addition, important percentage of evergreen broad-leaved trees and shrubs and number of warm temperate species and basal area were increased in the experiment plots while the introduced evergreen broad-leaved trees were declined.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.16 no.1
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• pp.49-55
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• 2020

Creep rupture data for Alloy 690 steam generator tubes in a pressurized water reactor are essentially needed to demonstrate a severe accident scenario on thermally-induced tube failures caused by hot gases in a damaged reactor core. The rupture data were obtained using the tube specimens under different applied-stress levels at 650℃, 700℃, 750℃, 800℃, and 850℃. Important creep constants were proposed using various creep laws in terms of Norton power law, Monkman-Grant (M-G) relation, damage tolerance factor (λ), and Zener-Hollomon parameter (Z). In addition, a creep activation energy (Q) value for Alloy 690 tube was reasonably determined using experimental data. Creep behaviors such as creep strength, creep rates, rupture elongation showed the results of temperature dependence well. Modified M-G plot improved a correlation of the creep rate and rupture life. Damage tolerance factor for Alloy 690 tubes was found to be λ =2.20 in an average value. Creep activation energy for Alloy 690 tube was optimized for Q=350 (kJ/mol). A plot of Z parameter obeyed a good linearity, and the same creep mechanism was inferred to be operative in the present test conditions.

• Smart Media Journal
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• v.8 no.1
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• pp.82-91
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• 2019

This study provides the analysis and prediction of fruits diseases related to weather conditions (temperature, wind speed, solar power, rainfall and humidity) using Linear Model and Poisson Regression. The main goal of the research is to control the method of fruits diseases and also to prevent diseases using less agricultural pesticides. So, it is needed to predict the fruits diseases with weather data. Initially, fruit data is used to detect the fruit diseases. If diseases are found, we move to the next process and verify the condition of the fruits including their size. We identify the growth of fruit and evidence of diseases with Linear Model. Then, Poisson Regression used in this study to fit the model of fruits diseases with weather conditions as an input provides the predicted diseases as an output. Finally, the residuals plot, Q-Q plot and other plots help to validate the fitness of Linear Model and provide correlation between the actual and the predicted diseases as a result of the conducted experiment in this study.

• Korean Journal of Environment and Ecology
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• v.28 no.1
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• pp.33-44
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• 2014

The purpose of this study was to provide basic information and investigate a vegetation structure around Tohamsan (Mt.) Wetland. Actual vegetation map was made on the basis of watershed around that. Vegetation structure survey was carried out for 8 representative communities of actual vegetation which were Pinus densifrora community, Quercus variabilis community, Cornus controversa community, Q. serrata community, Q. mongolica-Q. serrata community, Salix koreensis community, Q. mongolica community, Q. variabilis-Q. mongolica-Q. serrata community. Tohamsan (Mt.) Wetland is located on 490m above sea level and the area of watershed was $236,272m^2$. Vegetation type were divided into 16 types, and the ratio of Q. mongolica community was 33.1% ($78,209.2m^2$). In order to turn out the structure of 8 representative communties, 32 plots were set up and unit area of plot was $100m^2$. The estimated age of forest is 30~50-years-old, and in the resutls of soil analysis, acidity was pH 4.89 and organic matter was 4.46%.

• Korean Journal of Environment and Ecology
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• v.12 no.3
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• pp.279-289
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• 1998

To monitor vegetation structure of evergreen broad-leaved forest, twenty plots were set up at Wando Arboretum in 1996. Twenty plots were divided into ten experimental plots and control plots. A few evergreen broad-leaved species were planted after selective cutting at ten experimental plots. Dominant species of twenty plots were Quercus acuta, Q. acuta and Carpinus tschonoskii, Pinus densiflora, etc.. Vegetation structure of the experimental plots showed seral stage of succession from P. densiflora community or deciduous broad-leaved forest to Q. acuta community. Dominant species of the experimental plots were changed to Q. acuta or P. densiflora after selective cutting. And a number of species and individuals, coverages etc. were increased at shrub and ground cover layer after treatment.

• The Korean Journal of Quaternary Research
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• v.17 no.2
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• pp.163-163
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• 2003

The study area, Kwangneung Experiment Forest (KEF) is located on the west-central portion of Korean peninsula and belongs to a cool-temperate broadleaved forest zone. At the old-growth deciduous forest near Soribong-peak (533.1m) in KEF, we have established a permanent plot and a flux tower, and the site was registered as a KLTER site and also a KoFlux site. In this study, we aimed to present basic ecological characteristics and synthetic data of carbon budgets and flows, and some monitoring data which are essential for providing important parameters and validation data for the forest dynamics models or biogeochemical dynamics models to predict or interpolate spatially the changes in forest ecosystem structure and function. We made a stemmap of trees in 1 ha plot and analyzed forest stand structure and physical and chemical soil characteristics, and estimated carbon budgets by forest components (tree biomass, soils, litter and so on). Dominant tree species were Quercus serrata and Carpinus laxiflora, and accompanied by Q. aliena, Carpinus cordata, and so on. As a result of a field survey of the plot, density of the trees larger than 2cm in DBH was 1,473 trees per ha, total biomass 261.2 tons/ha, and basal area 28.0 m2/ha. Parent rock type is granite gneiss. Soil type is brown forest soil (alfisols in USDA system), and the depth is from 38 to 66cm. Soil texture is loam or sandy loam, and its pH was from 4.2 to 5.0 in the surface layer, and from 4.8 to 5.2 in the subsurface layer. Seasonal changes in LAI were measured by hemispherical photography at the l.2m height, and the maximum was 3.65. And the spatial distributions of volumetric soil moisture contents and LAIs of the plot were measured. Litterfall was collected in circular littertraps (collecting area： 0.25m2) and mass loss rates and nutrient release patterns in decomposing litter were estimated using the litterbag technique employing 30cm30cm nylon bags with l.5mm mesh size. Total annual litterfall was 5,627 kg/ha/year and leaf litter accounted for 61% of the litterfall. The leaf litter quantity was highest in Quercus serrata, followed by Carpinus laxiflora and C. cordata, etc. Mass loss from decomposing leaf litter was more rapid in C. laxiflora and C. cordata than in Q. serrata litter. About 77% of C. laxiflora and 84% of C. cordata litter disappeared, while about 48% in Q. serrata litter lost over two years. The carbon pool in living tree biomass including below ground biomass was 136 tons C/ha, and 5.6 tons C/ha is stored in the litter layer, and about 92.0 tons C/ha in the soil to the 30cm in depth. Totally more than about 233.6 tons C/ha was stored in DK site. And then we have drawn a schematic diagram of carbon budgets and flows in each compartment of the KEF site.

• Journal of Animal Science and Technology
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• v.49 no.5
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• pp.559-568
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• 2007

Recently, copy number variations (CNV) of genes or genomic segments have been intensively studied and various analysis methods have been developed. In this study, quantitative oligonucleotide ligation assay (qOLA) was applied to investigate CNV of KIT gene in the Landrace breed. A combined assay using qOLA and pyrosequencing, 6 genotype classes, I1/I1 or I3/i (IBe), I1/I2 or I3/IP, I1/I3, I1/IP or I2/i (IBe), I2/I2and I2/IP, were identified from 44 Landrace pigs. Genotype assignment using grouping features of measurements on a scatter plot showed 100% agreement with those using a statistical assignment by PROC FASTCLUS procedure implemented in the SAS package. Two versions (3100 and 3130) of ABI sequencers gave the same genotyping results, indicating there was no influence on qOLA by different versions of instrument, however, the means of standard deviation and coefficient of variation from the qOLA on a ABI 3130 (2.33 and 4.10) was lower than those from the qOLA on a ABI 3100 (2.67 and 4.81). Effect of proteinase K treatment on the PCR product followed by qOLA was very clear because noise peaks were disappeared and the observed ration fit better to the reference ratio corresponding to each genotype.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.11C
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• pp.1062-1069
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• 2003

This paper presents a new loop shaping technique for design of robust optimal PID controllers in order to satisfy the performance requirements. PID controller can be designed by selecting the suitable weighting factors Q and R. This technique is developed by pushing all two zeros formed by PID controller closely to a larger pole of the second order plant. As a result, a good loop shaping is achieved in the high frequencies region on the Bode plot. For the robust optimal tuning of PID controller for second order system, a new loop shaping procedure is developed via LQR approach.

• Journal of Ginseng Research
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• v.10 no.2
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• pp.180-186
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• 1986

Photosynthesis and respiration of leaf and root of field grown Panax ginseng were investigated according to aerial part sensecence. No apparent photosynthesis activity was detected in senescenced leaf(less than 0.7mg total chlorophyll/g FW) and leaf dark respiration was consistent relation with senescence. Leaf respiratory Q$_{10}$ consistently increased with senscence. Root respiration and Q$_{10}$ tended to decrease with aerial part senescence only in the range of optimum temperature of leaf growth. Apparent photosynthesis or respiration of leaf was negatively or positively correlated, respectively with the increase of air temperature. Root respiration with temperature was well accordance with Arrhenius plot which was not consistent with aerial part senescence. Accelerated senescence may be recommendable for better root yield unless any reserve in stem or leaves contributes to root through translocation.