• Title/Summary/Keyword: Q&A System

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SKF96365 impedes spinal glutamatergic transmission-mediated neuropathic allodynia

  • Qiru Wang;Yang Zhang;Qiong Du;Xinjie Zhao;Wei Wang;Qing Zhai;Ming Xiang
    • The Korean Journal of Physiology and Pharmacology
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    • v.27 no.1
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    • pp.39-48
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    • 2023
  • Spinal nerve injury causes mechanical allodynia and structural imbalance of neurotransmission, which were typically associated with calcium overload. Storeoperated calcium entry (SOCE) is considered crucial elements-mediating intracellular calcium homeostasis, ion channel activity, and synaptic plasticity. However, the underlying mechanism of SOCE in mediating neuronal transmitter release and synaptic transmission remains ambiguous in neuropathic pain. Neuropathic rats were operated by spinal nerve ligations. Neurotransmissions were assessed by whole-cell recording in substantia gelatinosa. Immunofluorescence staining of STIM1 with neuronal and glial biomarkers in the spinal dorsal horn. The endoplasmic reticulum stress level was estimated from qRT-PCR. Intrathecal injection of SOCE antagonist SKF96365 dose-dependently alleviated mechanical allodynia in ipsilateral hind paws of neuropathic rats with ED50 of 18 ㎍. Immunofluorescence staining demonstrated that STIM1 was specifically and significantly expressed in neurons but not astrocytes and microglia in the spinal dorsal horn. Bath application of SKF96365 inhibited enhanced miniature excitatory postsynaptic currents in a dosage-dependent manner without affecting miniature inhibitory postsynaptic currents. Mal-adaption of SOCE was commonly related to endoplasmic reticulum (ER) stress in the central nervous system. SKF96365 markedly suppressed ER stress levels by alleviating mRNA expression of C/ EBP homologous protein and heat shock protein 70 in neuropathic rats. Our findings suggested that nerve injury might promote SOCE-mediated calcium levels, resulting in long-term imbalance of spinal synaptic transmission and behavioral sensitization, SKF96365 produces antinociception by alleviating glutamatergic transmission and ER stress. This work demonstrated the involvement of SOCE in neuropathic pain, implying that SOCE might be a potential target for pain management.

Physiological and transcriptome analysis of acclimatory response to cold stress in marine red alga Pyropia yezoensis

  • Li-Hong Ma;Lin Tian;Yu-Qing Wang;Cong-Ying Xie;Guo-Ying Du
    • ALGAE
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    • v.39 no.1
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    • pp.17-30
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    • 2024
  • Red macroalga Pyropia yezoensis is a high valuable cultivated marine crop. Its acclimation to cold stress is especially important for long cultivation period across winter in coasts of warm temperate zone in East Asia. In this study, the response of P. yezoensis thalli to low temperature was analyzed on physiology and transcriptome level, to explore its acclimation mechanism to cold stress. The results showed that the practical photosynthesis activity (indicated by ΦPSII and qP) was depressed and pigment allophycocyanin content was decreased during the cold stress of 48 h. However, the Fv/Fm and non-photochemical quenching increased significantly after 24 h, and the average growth rate of thalli also rebounded from 24 to 48 h, indicating a certain extent of acclimation to cold stress. On transcriptionally, the low temperature promoted the expression of differentially expressed genes (DEGs) related to carbohydrate metabolism and energy metabolism, while genes related to photosynthetic system were depressed. The increased expression of DEGs involved in ribosomal biogenesis and lipid metabolism which could accelerate protein synthesis and enhance the degree of fatty acid unsaturation, might help P. yezoensis thallus cells to cope with cold stress. Further co-expression network analysis revealed differential expression trends along with stress time, and corresponding hub genes play important roles in the systemic acquired acclimation to cold stress. This study provides basic mechanisms of P. yezoensis acclimation to cold temperature and may aid in exploration of functional genes for genetic breeding of economic macroalgae.

Expression of SARS-3CL Protease in a Cell-Free Protein Synthesis System (무세포 단백질 합성법을 이용한 활성형 SARS-3CL protease의 발현)

  • Park, Sun-Joo;Kim, Yong-Tae
    • Journal of Life Science
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    • v.22 no.4
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    • pp.552-558
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    • 2012
  • Severe acute respiratory syndrome (SARS) is a severe respiratory infectious disease caused by a novel human coronavirus, SARS-CoV. The 3CL protease is a key enzyme in the proteolytic processing of replicase polyprotein precursors, pp1a and pp1ab, which mediate all the functions required for viral genomic replication and transcription. Therefore, this enzyme is a target for the development of chemotherapeutic agents against SARS. A large quantity of active SARS-3CL protease is required for development of anti-SARS agents. Here we have constructed overexpression vector for the production of the SARS-3CL protease. The gene encoding SARS-3CL protease was amplified using polymerase chain reaction and cloned into the pET29a expression vector, resulting in pET29a/SARS-3CLP. Recombinant SARS-3CL protease was successfully synthesized by the dialysis mode of the cell-free protein expression system, and purified by three-step fast protein liquid chromatography using HighQ and MonoP column chromatographies and Sephacryl S-300 gel filtration. In addition, the produced SARS-3CL protease was found to be an active mature form. This study provides efficient methods not only for the development of anti-SARS materials from natural sources, but also for the study of basic properties of the SARS-3CL protease.

Crystal structure refinement and microwave dielectric characteristic of $(1-x)CaTiO_3-x(La_{1/3}Nd_{1/3})TiO_3$ ($(1-x)CaTiO_3-x(La_{1/3}Nd_{1/3})TiO_3$계의 결정구조 해석 및 마이크로파 유전 특성)

  • 조남웅;성경필;문종하;최주현
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.8 no.3
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    • pp.478-486
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    • 1998
  • $CaTiO_3-x(La_{1/3}Nd_{1/3}TiO_3\;(0\le \textrm x\le0.8)$ system was prepared by reaction of $CaCO_3,\;LaO_3,\;Nd_2CO_3$ and ,TEX>$TiO_2$ mixture at 1673 K, which can be applied for microwave dielectric ceramic materials. The lattice parameters of(1-x))$CaTiO_3-x(La_{1/3}Nd_{1/3}TiO_3\;(0\le \textrm x\le0.8)$ system increased with the increase of x. Its structure was investigated by Rietveld profile-analysis of XRD in detail. Cations $ La^{3+}$ and Nd^{3+}$ were located at the $Ca^{2+}$ site in the range of $0\le \textrm x\le0.8$. crystal structure in $;(0\le \textrm x\le0.6)$ maintained space group Pnma with CaTiO_3 structure. The tiled and distorted $TiO_6$ was gradually released with the increase of x in $0\le \textrm x\le0.6$ .The structure was changed to a new space group of $Pmn2_1$ at the x value of 0.8. The relative dielectric constant $(\epsilon_r)$ of $(1-x)CaTiO_3-x(La_{1/3} Nd_{1/3})TiO_3$ ($(0\le \textrm x\le0.8)$) system was exponentially decreased by with the increased of x. The temperature coefficient of resonant frequency $(\tau_f)$ decreased with the increase of x in $0\le \textrm x\le0.6$ and then increased again at x=0.8 due to the change of crystal structure. The value of Q$\cdot f_o$ was 13800 (GHz) at x=0.2 and was very low under 2000 (GHz) in 0.4$\leq$x$\leq$0.8.

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Strength and failure characteristics of the rock-coal combined body with single joint in coal

  • Yin, Da W.;Chen, Shao J.;Chen, Bing;Liu, Xing Q.;Ma, Hong F.
    • Geomechanics and Engineering
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    • v.15 no.5
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    • pp.1113-1124
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    • 2018
  • Geological dynamic hazards during deep coal mining are caused by the failure of a composite system consisting of the rock and coal layers, whereas the joint in coal affects the stability of the composite system. In this paper, the compression test simulations for the rock-coal combined body with single joint in coal were conducted using $PFC^{2D}$ software and especially the effects of joint length and joint angle on strength and failure characteristics in a rock-coal combined body were analyzed. The joint length and joint angle exhibit a deterioration effect on the strength and affect the failure modes. The deterioration effect of joint length of L on the strength can be neglected with a tiny variation at ${\alpha}$ of $0^{\circ}$ or $90^{\circ}$ between the loading direction and joint direction. While, the deterioration effect of L on strength are relatively large at ${\alpha}$ between $30^{\circ}$ and $60^{\circ}$. And the peak stress and peak strain decrease with the increase of L. Additionally, the deterioration effect of ${\alpha}$ on the strength becomes larger with the increase of L. With the increase of ${\alpha}$, the peak stress and peak strain first decrease and then increase, presenting "V-shaped" curves. And the peak stress and peak strain at ${\alpha}$ of $45^{\circ}$ are the smallest. Moreover, the failure mainly occurs within the coal and no apparent failure is observed for rock. At ${\alpha}$ between $30^{\circ}$ and $60^{\circ}$, the secondary shear cracks generated in or close to the joint tips, cause the structural instability failure of the combined body. Therefore, their failure models present as a shear failure along partial joint plane direction and partially cutting across the coal body or a shear failure along the joint plane direction. However, at ${\alpha}$ of $60^{\circ}$ and L of 10 mm, the "V-shaped" shear cracks cutting across the coal body cause its final failure. While crack nucleations at ${\alpha}$ of $0^{\circ}$ or $90^{\circ}$ are randomly distributed in the coal, the failure mode shows a V-shaped shear failure cutting across the coal body.

A Study on the Integration of Information Extraction Technology for Detecting Scientific Core Entities based on Large Resources (대용량 자원 기반 과학기술 핵심개체 탐지를 위한 정보추출기술 통합에 관한 연구)

  • Choi, Yun-Soo;Cheong, Chang-Hoo;Choi, Sung-Pil;You, Beom-Jong;Kim, Jae-Hoon
    • Journal of Information Management
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    • v.40 no.4
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    • pp.1-22
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    • 2009
  • Large-scaled information extraction plays an important role in advanced information retrieval as well as question answering and summarization. Information extraction can be defined as a process of converting unstructured documents into formalized, tabular information, which consists of named-entity recognition, terminology extraction, coreference resolution and relation extraction. Since all the elementary technologies have been studied independently so far, it is not trivial to integrate all the necessary processes of information extraction due to the diversity of their input/output formation approaches and operating environments. As a result, it is difficult to handle scientific documents to extract both named-entities and technical terms at once. In this study, we define scientific as a set of 10 types of named entities and technical terminologies in a biomedical domain. in order to automatically extract these entities from scientific documents at once, we develop a framework for scientific core entity extraction which embraces all the pivotal language processors, named-entity recognizer, co-reference resolver and terminology extractor. Each module of the integrated system has been evaluated with various corpus as well as KEEC 2009. The system will be utilized for various information service areas such as information retrieval, question-answering(Q&A), document indexing, dictionary construction, and so on.

Implement of Job Reinforcement System based on Google Cloud for Efficient Job Preparations and Self Ability Improvement (효율적인 취업 준비와 자기 능력 향상을 위한 구글 클라우드 기반의 취업 강화 시스템 구현)

  • Kang, Joo-hee;Yang, Byeong-ryeol;Jung, Se-hoon;Kim, Jong-chan;Park, Hong-joon;So, Won-ho;Sim, Chun-bo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.05a
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    • pp.756-759
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    • 2015
  • As the struggle to get a job becomes increasingly ferocious year after year, the website offering a range of employment information have continued to gain popularity. Those website, however, are not of much help when it comes to an interview to face the interviewer. Furthermore, many people suffer depression due to the heavy stress they are under while making preparations for employment or working for an organization. The current employment website do not have a team of professionals to deal with such issues. In an effort to solve those problems, the present study made arrangements to allow users to put in an enormous amount of employment information based on Google App Engine(GAE) and Google Web Toolkit(GWT) and upload interview videos. In addition, a Q&A board was created to help those who prepare for employment or current workers relieve their stress and provide them with access to a team of counseling professionals.

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The Historical Study of Headache in Chinese Ming Dynasty (명대의가(明代醫家)들의 두통(頭痛)에 대한 인식변화에 관한 연구)

  • Chun, Duk-Bong;Maeng, Woong-Jae;Kim, Nam-Il
    • The Journal of Korean Medical History
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    • v.24 no.1
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    • pp.43-56
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    • 2011
  • Everyone once in a life experience headaches as symptoms are very common. According to a study in a country of more than a week and as many as those who have experienced a headache amounts to 69.4%. In addition, the high reported prevalence of migraine in 30s for 80% of all migraine sufferers daily life interfere with work or was affected. In Western medicine, the cause of headaches is traction or deformation of pain induced tissue like scalp, subcutaneous tissue, muscle, fascia, extracranial arteriovenous, nerves, periosteum. But it turns out there are not cause why pain induced tissue is being tracted or deformated. Therefore, most of the western-therapy is mainly conducted with regimen for a temporary symptom reduction. Therefore, I examined how it has been developed in Chinese Ming Dynasty, the perception of headache, change in disease stage and an etiological cause. Oriental medicine in the treatment of headache is a more fundamental way to have an excellent treatment. The recognition of head in "素問($s{\grave{u}}$ $w{\grave{e}}n$)" and "靈樞($l{\acute{i}}ng$ $sh{\bar{u}}$)" began to appear in 'Soul-神($sh{\acute{e}}n$) dwelling place' and 'where to gather all the Yang-'諸陽之會($zh{\bar{u}}$ $y{\acute{a}}ng$ $zh{\bar{i}}$ $hu{\grave{i}}$)'. Also, head was recognized as '六腑($li{\grave{u}}f{\check{u}}$) 淸陽之氣($q{\bar{i}}ng$ $y{\acute{a}}ng$ $zh{\bar{i}}$ $q{\grave{i}}$) and 五臟($w{\check{u}}$ $z{\grave{a}}ng$) 精血($j{\bar{i}}ng$ $xu{\grave{e}}$) gathering place'. More specific structures such as the brain is considered a sea of marrow(髓海-$su{\check{i}}$ $h{\check{a}}i$) in "內經($n{\grave{e}}i$ $j{\bar{i}}ng$)" and came to recognized place where a stroke occurs. Accompanying development of the recognition about head, there had been changed about the perception of headache and the recognition of the cause and mechanism of headache. And the recognition of headache began to be completed in Ming Dynasty through Jin, Yuan Dynasty. Chinese Ming Dynasty, specially 樓英($l{\acute{o}}u$ $y{\bar{i}}ng$), in "醫學綱目($y{\bar{i}}xu{\acute{e}}$ $g{\bar{a}}ngm{\grave{u}}$)", first enumerated prescription in detail by separating postpartum headache. and proposed treatment of headache especially due to postpartum sepsis(敗血-$b{\grave{a}}i$ $xu{\grave{e}}$). 許浚($x{\check{u}}$ $j{\grave{u}}n$) accepted a variety of views without impartial opinion in explaining one kind of headache in "東醫寶鑑($d{\bar{o}}ng-y{\bar{i}}$ $b{\check{a}}oji{\grave{a}}n)$" 張景岳($zh{\bar{a}}ng$ $j{\check{i}}ng$ $yu{\grave{e}}$), in "景岳全書($j{\check{i}}ng$ $yu{\grave{e}}$ $qu{\acute{a}}nsh{\bar{u}}$)", established his own unique classification system-新舊表裏($x{\bar{i}}nji{\grave{u}}$ $bi{\check{a}}ol{\check{i}}$)-, and offered a clear way even in treatment. Acupuncture treatment of headache in the choice of meridian has been developed as a single acupuncture point. Using the classification of headache to come for future generation as a way of locating acupoints were developed. Chinese Ming Dynasty, there are special treatments like 導引按蹻法($d{\check{a}}o$ y ${\check{i}}n$ ${\grave{a}}n$ $ji{\check{a}}o$ $f{\check{a}}$), 搐鼻法($ch{\grave{u}}$ $b{\acute{i}}$ $f{\check{a}})$, 吐法($t{\check{u}}$ $f{\check{a}}$), 外貼法($w{\grave{a}}i$ $ti{\bar{e}}$ $f{\check{a}}$), 熨法($y{\grave{u}}n$ $f{\check{a}}$), 點眼法($di{\check{a}}n$ $y{\check{a}}n$ $f{\check{a}}$), 熏蒸法($x{\bar{u}}nzh{\bar{e}}ng$ $f{\check{a}}$), 香氣療法($xi{\bar{a}}ngq{\grave{i}}$ $li{\acute{a}}of{\check{a}}$). Most of this therapy in the treatment of headache, it is not used here, but if you use a good fit for today's environment can make a difference.

Content Analysis of the Health Counseling by the Intranet in University : 2000-2004 (일개 대학교 5년간 인트라넷 건강상담 내용분석)

  • Kim, Hyeung-Dae;Bae, Seok-Hwan;Na, Bak-Ju;Kim, Keon-Yeop;Kim, Chul-Woung;Kang, Moon-Young;Kim, Dae-Kyung;Oh, Kyung-Hee;Lee, Moo-Sik
    • Journal of agricultural medicine and community health
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    • v.32 no.2
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    • pp.75-86
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    • 2007
  • Objectives: The research was aimed at analyzing the contents of university intranet for systematically execution of the healthy information provision and healthy consultation services from 2000 January to 2004 December. Methods: We have analyzed 300(28.3%) the instances of accomplished health consultation cases from the whole 1,059 instances which were the replied in a university intranet. Results: According to the contents of health consultation in ICPC code, a general symptom 91 cases(30.3%) was most, muscle-skeletal system 44 cases(14.7%), and digestive system 43 cases(14.3%) in order of cases of health counselling. The symptoms and complaint with 155 cases(51.7%) were most in the distribution of the health counselling contents by 17 charter of ICPC. The most common reason of counselling by 17 charter of ICPC were as follows; questions about the symptom and diagnosis(118 cases, 39.3%), the preventive and treatment methods(91 cases, 30.0%), and medical fee(20cases, 6.7%) in order. We mainly answer on the content of health counselling were as follows; make an offer of medical information and knowledge(48.3%), recommend visit clinic or hospital(23.7%), guide to treatment(12.7%), and so on. Conclusions: This research showed that the program of health counselling may not meet completely the high quality and adequate distribution of health counselling by the intranet in a university by content analysis. The finding suggests that health counselling by intranet in a university may be used to supplement of systemic improvement on the intranet Q/A format from current lack of essential health information and security for the quality of the health counselling.

A Research Relative to the Subjective View on the Occupational Consciousness of the Dental Coordinator (Dental Coordinator의 직업의식에 관한 주관성 연구)

  • Han, Gyeong-Soon
    • Journal of Korean society of Dental Hygiene
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    • v.2 no.1
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    • pp.97-113
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    • 2002
  • This research is an attempt to establish a basic material in discovering the subjective types of the dental coordinators occupational consciousness, utilizing the Q-Method Theory, which is capable of measuring the subjective nature of human being by scientific and systematic method. The characteristics of each type were analyzed so as to have the dental coordinator to possess the desirable understanding of the occupational view together for them to have self-confidence as a lifetime specialty occupation. Upon completion of the study, it was found that there are three types of subjective views pertaining to the occupational consciousness on the dental coordinator. The result on the analysis of the characteristics is as follows : Type 1 is the Taking Serious View on the Patient Service, which priority is placed on kindness and looking after any difficulties of the patient, namely, it is the type that puts the importance on the high level medical examination and treatment based on good service. Because of the kind service to the patient, those under this category considered their job satisfaction have been improved. It goes without saying that they were developing the services, which satisfy themselves, and it was confirmed that they thought that they should make things smooth for any development of problems that may arise between the patient and the dentist. Type 2 is the Taking Serious View on Career and Organizational Strength. This is a type that aims on the systematic nature of the internal job, namely, organizational strength itself viewed from the stand point thai either self-confident or work merit is proportional to experience. This type is to actively treat the problem and endeavor to solve the issue when dissatisfaction is held by the dental hospital system, which is either followed according to the work responsibility or by an organization having an appropriate system on remuneration, Type 3 is the Placing of Importance on the Overall Circumstances. This is the type that places importance on the overall now of the dental hospital, and it is the type, that most greatly recognizes the importance of the role of the dental coordinator. The dental coordinator should provide the sense of reliability to both the dentist and to the staff of the medical examination and treatment and should be able to adjust smoothly the overall now of the medical treatment. The dental coordinator is expected to be a medical service specialist, who is capable to solve the problems of the staff of the medical treatment intuitively and to adjust the relation between the employees. With the above result, it was found that although there was no mutual exclusiveness between the respective types, there are particular characteristics among each type. Therefore, this research has discovered and analyzed the subjective view types relative to the occupational consciousness of the dental coordinator for the proper understanding on the characteristics of each type to enable the dental sanitary students and the graduates of the Dental Sanitary Department, who are aspiring to become dental coordinator, as the basic guide material to be actively utilized.

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