• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.622-624
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• 1998

During the course of study on plant parasitic nematodes associated with medicinal herbs in Korea, high population of different stages of citrus nematode, Tylenchulus semipentrans was found in association with Pyrola japonia Klenze in Imsil, Chunbuk province. The second stage larvae and males were isolated from rhizosphere soil ; immature and mature females were detached from the roots of infested plants of Pyrola japonica. Besides citrus it has been reported to parasitize on roots of Syringa vulgaris, Diospyros lotus, olive, grapevine, and pear in different parts of the world. This is the first record of T. semipenetrans parasitized on a medicinal plant, Pyrola japonica.

• Korean Journal of Pharmacognosy
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• v.38 no.1
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• pp.45-49
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• 2007

Chinese crude drug "Lu-ti-cao"(鹿蹄草) has been used as a remedy for beriberi, cut bleeding, snake bite, etc. In Korea, the drug is called "Nok Je Cho" and has been used as a folk medicine of the same effect as that of Chinese equivalent. The original plant of this drug has not yet been as certained. To clarify the botanical origin of "Nok Je Cho", the morphological and anatomical characteristics of the leaves of Pyrola species growing in Korea, i.e. P. incarnata, P. japonica, P. minor, P. renifolia, P. secunda were studied. As a result, it was clarified that "Nok Je Cho" was the leaf of Pyrola japonica and Pyrola minor.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.193-200
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• 2007

Pollen grains of seven taxa on the family Pyrolaceae, known to be distributed in Korea (Pyrola dahurica(Andreses) Kom., P. incarnata (DC.) Fisch. ex Kom., P. japonica Klenze ex Alefeld, P. minor L., P. renifolia Max., Orthilia secunda (L.) House, Chimaphila japonica Miq.), were examined by the light and the scanning electron microscope in order to evaluate their taxonomic significances. The pollen units of genus Pyrola, Orthilia, Chimaphila were tetrad, monad, polyad respectively. This characteristics was good character for delimiting the genus, and Orthilia secunda (L.) House was more proper scientific name than Pyrola secunda L. based upon this character. Pollen size, tricolporate length, surface sculpturing pattern were different slightly among the treated taxa, and surface sculpturing patterns of Pyrola dahurica, P. incarnate, P. renifolia, Chimaphiza japonica were different from previous reports. Evolutionary trends of pollen grains in Pyrolaceae were inferred from the pollen units and aperture shapes.

• The Korean Journal of Ecology
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• v.22 no.6
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• pp.349-354
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• 1999

The spatial distributions of allelic frequencies and ecological traits by randomization were studied in the natural population of two species (Pinus rigida and Pyrola japonica). Both species showed significant positive spatial autocorrelation as measured by Moran's I. In P. rigida, the genetic similarity was shown in individuals within up to a scale of 18 m distance and this is partly due to combination of pollen and seed dispersal by wind or men. In P. japonica, significant spatial autocorrelation was consisted of a scale of 8 m intervals. These population structure in the distribution of allelic frequencies is related to mating systems such as outcrossing and vegetative spread. The results also indicate that positive species associations between P. rigida and P. japonica can occur when both species select the same habitat or require the same environmental conditions.

• Journal of Life Science
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• v.16 no.6
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• pp.989-993
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• 2006

The antimicrobial substance from Pyrola japonica were extracted and isolated. Eighty percent ethanol extract of dried Pyrola japonica was fractionated to hexane, diethyl ether, ethyl acetate and aqueous layer. The hexane-soluble fraction showed the highest inhibitory activity against Bacillus subtilis. Moreover the hexane layer was fractionated into 5 groups by silica gel column chromatography. From the results, group No. 2 ($18{\sim}40$ fractions) showed the highest antimicrobial activity. The group was re-separated to 10 fractions by preparative thin layer chromatography and the peak I as active fraction was isolated by HPLC.

• Archives of Pharmacal Research
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• v.24 no.6
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• pp.522-523
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• 2001

A new naphthoquinone, 5,8-dihydro-2,7-dimethyl-[1,4]naphthoquinone (1), which was named 5,8-dihysrochimaphilin, isolated from an ethyl acetate soluble fraction from the root of Pyrola japonica, together with chimaphilin (2). Compound 1 was transformed rapidly to 2 upon exposure to air by HPLC analysis. This fact supported that chimaphilin (2) may be an artifact from 1 .

• The Korean Journal of Ecology
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• v.13 no.1
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• pp.59-66
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• 1990

Changes in water potential, lethal temperature and carbohydrate content in the leaves of wintergreen (Pyrola japonica) during overwintering were investigated. Leaf water potential was kept at -2 bars in the tender stage before October, decreased to -46 bars in the dormancy stage and increased to -2 bars again after dehardening Lethal temperatures of the leaf tissue were $-7^{\circ}C$ in the tender stage and $-7^{\circ}C$ in the dormancy stage, but did not recover up to that of the tender stage during dehardeding. Peak of soluble sugar content coincided with the nadir of the leaf water potential. There were close relationships among daily minimum air-temperature, leaf water potential and lethal temperature in changing patterns during overwintering.

• YAKHAK HOEJI
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• v.40 no.2
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• pp.225-229
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• 1996

The cytotoixic effect of Pylorae Herba (Pyrola japonica Klenze) against L1210 and K-562 cells was studied in vitro. The methanolic extract of Pylorae Radix was add ed to the culture of L1210 cells and K-562 cells for the cytotoxic activity and the ED50 values of hexane, ethylacetate, buthanol and water fractions from methanolic extract were determined using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay. The active constituent isolated by bioassay guided fractionation followed by purification gave rise to a yellow needle crystal and was clarified to be chimaphilline by the comparison with the published data. The average life spans with it were not prolonged significantly on tumor growth in hybrid female mouse (BDF1-KIST) inoculated subcutaneously with P388 cells.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.256.2-256.1
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• 2003

Six known phenolic glycosides. hyperin(2), androsin(3), homoarbutin(4), isohomoarbutin(4a), pirolatin(7) and monotropein(6), together with two new compounds, (1)[mp. 215 - 217$^{\circ}C$, C$\sub$23/H$\sub$32/O$\sub$11/] and (5)[mp. 121 -123$^{\circ}C$, C$\sub$18/H$\sub$26/O$\sub$8/] were isolated from the BuOH fraction of Pyrola japonica(Pyrolaceae). The structures of the known compounds were determined by chemical and spectroscopic methods. (omitted)

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.291.1-291
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• 1997

No Abstract, See Full Text