• Journal of Animal Science and Technology
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• 제45권4호
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• pp.523-528
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• 2003

본 연구는 PvuII PCR-RFLP를 이용하여 Yorkshire종 돼지에서 Estrogen Receptor의 유전적 다형과 산자수간의 관련성을 분석하기 위하여 수행되었다. 무창 돈사에서 사육중인 242두의 종빈돈으로부터 혈액을 채취하여 PvuII PCR- RFLP로 ER 유전자형을 결정하였다. ER 유전자 좌위에서 유전자 빈도는 각각 0.39(A)와 0.61(B)였다. ER 유전자형별 산자수에 대한 효과는 최소제곱평균을 설명하는 고정모형을 설정하여 추정하였다. 분석결과 복당 총산자수와 실산자수에서 특정 ER 대립유전자(B)에서 산자수 증진효과가 관찰되었다. 따라서 돼지 ER 좌위의 유전적 변이는 번식돈의 산자수 증대와 관련된 marker-assisted selection(MAS)에 응용될 수 있을 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권9호
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• pp.1223-1226
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• 2004

Previously candidate gene approach revealed estrogen receptor (ESR) locus was associated with increased litter size. In this study, PvuII polymorphisms of ESR gene was detected by PCR-RFLP, and ESR locus was evaluated for its association with reproductive tracts components in the Large $White{\times}Meishan$ ($LW{\times}M$) F2 offspring. Ninety seven gilts with reproductive tracts components records and 136 offspring with performance traits records were genotyped and the results were used to estimate allele substitution effects. The results showed that two alleles (A and B) were identified, and 121 bp fragments were observed for the AA genotype and 65 bp and 56 bp fragments for the BB genotype; the length of uterine body (LUB) of BB gilts were significantly shorter than AA gilts', the additive effect was -1.762 cm; the uterine weight (UW) of AB gilts were significantly lighter than AA gilts' with the additive effect -18.058 g; no significant associations of ESR alleles with ovulation rate (OR), length of uterine horn (LUH), length of uterine cervix (LUC), weight of two ovaries (OW), volume of uterine lumen (VUL), length of oviduct (LO) were observed. BB genotypes gilts need significantly less days to 100 kg ($D_{100kg}$) than AA genotypes (p<0.01), the additive effect was per copy of B allele. Allele B is also favorable for average daily gain (ADG), with additive effect 0.015 kg/d (p<0.05). There was no difference between genotypes for backfat thickness at the 13th rib (SF13), loin meat height (ELMH), and loin meat percentage was estimated (ELMP), individual birth weight (IBW) and teat number (TN).