• 한국식품영양과학회지
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• 제27권6호
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• pp.1166-1172
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• 1998

A method for the isolation and purification of DNA from a red algae, Porphyra was innovated. The innovation of the method consists mainly of three steps that include sodium acetate treatment, chloroform extraction, and 0.2 volume isopropanol precipitation step. The sodium acetate treatment was designed to remove polysaccharide contamination, and the isopropanol step to remove proteins and salts contaminents. Genomic DNA,s of several species(for example, P. tenera, P. yezoensis, P. seriata, and P. pseudolinearis) was successfully isolated by the innovated method. The amount of DNA purified from one g of sample material with the innovated method was 53 g in average. The resulting DNA was characterized to include high molecular weight and showed no nuclease activity. The DNA was pure enough to be digested directly by various restriction enzymes without any difficulties. Porphyra DNA was pure enough and adequate for amplification reaction through the polymerase chain reaction (small nuclear rDNA PCR amplification).

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2000년도 추계학술대회논문집
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• pp.65-68
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• 2000

Polyethylene, has long history and is widely used, was researched due to good electrical properties by many authors. But PE under stress has the critical defects of space charge accumulation and tree growth, so various methods such as catalyst, additives and blend to improve these problems have been execute, of which we selected blending method. As in our previous papers we investigated electrical conduction, dielectric and AC dielectric breakdown characteristics, we did DC dielectric breakdown characteristics in this paper. We selected pure LLDPE, pure EVA and LLDPE films mixed with EVA as specimens, which were mixed with the weight percentages of 50, 60, 70 and 80[wt%] to be thin film. DC applying voltage speed was 500[V/sec]. The relation between dielectric breakdown characteristics and the variations of super structure due to mixing was investigated, and especially trap level at amorphous region, threshold energy increment of conductive electron at free volume were considered.

• 대한기계학회논문집
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• 제19권1호
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• pp.193-201
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• 1995

The role of thermal contact resistance between a casting and a metal mold as well as natural convection in the melt during solidification of a pure metal is numerically studied. Numerical simulation is performed for a rectangular cavity using the coordinate transformation by boundary-fitted coordinate and pure aluminum is used as the phase- change material. The influences of thermal contact resistance on the interface shape and position, solidified volume fraction, temperature field and local heat transfer are investigated.

• 한국식품위생안전성학회지
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• 제10권3호
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• pp.169-174
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• 1995

Piperine, component of pure ground black pepper, has strong stimulative and hot. Analytical method for piperine was developed by high performance liquid chromatography. Analytical conditions are as follows, mobile phase is 70% methanol, detector UV 343 nm (0.05 AuFs), column is Novapak 5 C18 (15 cm $\times$ 4.6mm), flow rate is 1.0ml/min, chart speed is 0.25 cm/min and injection volume is 20 ul. Analytical results are as follows that relative standard deviation is 1.15%, calibration curve is y=170473.1x-7848.5 (R2=0.999) that shows good linearity. Standard solution of piperine is stable up to 10 hr and content of piperine in pureground black pepper is 4.97$\pm$0.86% Retention time of piperine in HPLC method is about 7 min. Therefore, the developed HPLC method including simple pretreatment of sample will be contribute to quality mangement.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제6권2호
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• pp.110-115
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• 2006

In pure P2P environment, it is possible to provide service by using a little real-time information without using accumulated information. But in case of using only a little information that was locally collected, quality of recommendation service can be fallen-off. Therefore, it is necessary to study a method to improve qualify of recommendation service by using users' context information. But because a great volume of users' context information can be recognized in a moment, there can be a scalability problem and there are limitations in supporting differentiated services according to fields and items. In this paper, we solved the scalability problem by clustering context information per each service field and classifying it per each user, using SOM. In addition, we could recommend proper services for users by quantifying the context information of the users belonging to the similar classification to the service requester among classified data and then using collaborative filtering.

• 대한기계학회논문집B
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• 제31권1호
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• pp.16-20
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• 2007

In this paper, convective heat transfer and flow characteristics of $Al_2O_3$ nanoparticles suspended in water flowing through uniformly heated tubes are experimentally investigated under laminar flow regime. The heat transfer coefficient and the pressure drop of nanoparticles suspended in water are experimentally presented according to the pumping power. In addition, the heat transfer coefficient and the pressure drop of $Al_2O_3$ nanoparticles suspended in water are compared with those of pure water under the fixed pumping power. It is shown that the heat transfer coefficient of $Al_2O_3$ nanofluids with 0.1% volume fraction is enhanced by about 12% although the increment of the pressure drop of those is 4% compared with those of pure water.

• Structural Engineering and Mechanics
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• 제50권6호
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• pp.723-740
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• 2014

A fracture criterion based on the strain energy density (SED) over a control volume, which embraces the notch edge, is employed in the present paper to assess the fracture loads of some U-notched Brazilian disk (UNBD) specimens. The specimens are made of commercial graphite and have been tested under pure mode I, pure mode II and mixed mode I/II loading. The results show that the SED criterion allows to successfully assess the fracture loads of graphite specimens for different notch tip radii and various mode mixity conditions with discrepancies that fall inside the scatter band of ${\pm}20%$.

• 대한기계학회논문집A
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• 제32권5호
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• pp.379-386
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• 2008

Densification behavior of dissimilar material powder (copper and pure iron powder) under die compaction was investigated. Experimental data were obtained for copper and pure iron powder compacts with various volume ratios under die compaction. Dissimilar material powder was simultaneously compacted into a jointed cylindrical compact with different powder materials in inner and outer part, respectively. To simulate densification behavior of dissimilar material powder, elastoplastic constitutive equation proposed by Shima and Oyane was implemented into a finite element program (ABAQUS) under die compaction. Finite element results were compared with experimental data for densification, deformed geometry and density distribution of powder compacts under die compaction.

• 소성∙가공
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• 제22권8호
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• pp.450-455
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• 2013

In the current study, a new dynamic recrystallization model for predicting high temperature flow stress is developed based on a physical model and the mean field theory. In the model, the grain aggregate is assumed as a representative volume element to describe dynamic recrystallization. The flow stress and microstructure during dynamic recrystallization were calculated using three sub-models for work hardening, for nucleation and for growth. In the case of work hardening, a single parameter dislocation density model was used to calculate change of dislocation density and stress in the grains. For modeling nucleation, the nucleation criterion developed was based on the grain boundary bulge mechanism and a constant nucleation rate was assumed. Conventional rate theory was used for describing growth. The flow stress behavior of pure copper was investigated using the model and compared with experimental findings. Simulated results by cellular automata were used for validating the model.

• 대한기계학회논문집
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• 제10권6호
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• pp.923-928
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• 1986

본 논문에서는 열전도율이 큰 금속의 스크랩과 파라핀을 혼합한 축열 매질에 있어서 스크랩의 체적 혼합율에 따르는 열전도율의 변화를 모델링과 실험으로 구해서 축열 계산에 필요한 열전도율 자료를 제시하고자 한다.