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Production of Aminolevulinic Acid by Recombinant Escherichia coli Co-expressing hemA and otsBA Using Crude Glycerol as Carbon Source (폐글리세롤을 탄소원으로 hemA와 otsBA를 공동 발현하는 재조합대장균 배양을 통한 아미노레블린산 생산)

  • Yan, Jingmei;Pham, Diep Ngoc;Kang, Dae-Kyung;Kim, Sung Bae;Kim, Chang-Joon
    • Microbiology and Biotechnology Letters
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    • v.44 no.3
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    • pp.341-348
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    • 2016
  • This study aimed to develop a microbial process for producing aminolevulinic acid (ALA) using crude glycerol. In the culture of ALA-producing cells (Escherichia coli/pH-hemA) in a medium containing crude glycerol, the cell density and production were 1.8-fold and 1.2-fold lower than those obtained from pure glycerol, respectively. However, the cell growth and production were improved by supplementing the medium with trehalose (30 or 100 g/l). Engineered cells (E. coli/pH-hemA/pS-otsBA) were constructed to express otsBA and their culture performance was compared with that of control cells (E. coli/pH-hemA/ pSTV28). The effects of isopropyl β-D-1-thiogalactopyranoside (IPTG) concentration and the time of induction were examined to improve the cell growth and ALA production in engineered cells cultured using crude glycerol. When 0.6 mM of IPTG was added at the beginning of the exponential growth phase, the ALA produced by cells was 2,121 mg/l, which was comparable to that from pure glycerol. The results demonstrate that otsBA expression endowed cells with the capacity to tolerate the toxicity of crude glycerol for direct use.

Development of a Fuel Cell System Model for a Small Ship (소형 선박용 연료전지 시스템 모델 개발)

  • Bang, Eun-Shin;Kim, Young-Min;Kim, Myoung-Hwan;Park, Sang-Kyun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.26 no.5
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    • pp.569-575
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    • 2020
  • In this study, a fuel cell system model for ship power was developed and verified by comparing the experimental results obtained by supplying pure oxygen. To verify the proposed model, the fuel cell output characteristics when oxygen was supplied were compared with those when air was supplied using an air compressor. In addition, the effect of the change in the thermal properties of the fuel cell system on the output of the stack was examined. Within the experimental range of this study, when pure oxygen was supplied as the cathode supply gas, the calculated and experimental voltages and outputs obtained through modeling were almost the same over the entire load range. When air was supplied instead of oxygen for the cathode supply at a constant load of 560 A, each stack voltage was approximately 14 V, the stack output was approximately 8 kW, and the stack efficiency was approximately 3 %. It was confirmed that the overall system efficiency was reduced by approximately 8 %. Among the thermal properties examined in this study, the heat transfer coefficient of the coolant to the stack was found to have the greatest effect on the output of the stack.

Efficient shoot regeneration using cotyledon explants and Agrobacterium tumefaciens - mediated transformation of bottle gourd (Lagenaria sicraria Standl.) (박 자엽조직을 이용한 효율적인 식물체 분화와 Agrobacterium에 의한 형질전환)

  • Kim, Soo-Yun;Ahn, Yul-Kyun;Huh, Yun-Chan;Lee, Hye-Eun;Kim, Do-Sun
    • Journal of Plant Biotechnology
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    • v.41 no.3
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    • pp.140-145
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    • 2014
  • This study were carried out for selection of proper transformation variety and development of efficient regeneration and transformation methods. The number of shoot in commercial varieties of gourd plant were 0 ~ 7.3. and fusarium wilt resistant pure lines were 2.0 ~ 6.5 per dish containing on MS medium supplemented with 3 mg/L BA. The shoot regeneration frequency of fusarium wilt resistant pure lines were wide variation on the deviation. The expression of GFP was high 67% and 100% at the co-cultivation with Agrobacterium. The effective shoot regeneration plant hormone were combination BA and 2,4-D. The number and elongation condition of shoot was good after 4 weeks change with MS medium supplemented with 1 mg/L BA. Effective callus production plant hormone were combination of 3 mg/L BA and 0.1 mg/L 2.4-D.

Experimental Study of Removing Epoxy Resin from Iron Object using Nd:YAG Laser Cleaning System (철제유물에 사용된 에폭시수지 제거를 위한 Nd:YAG 레이저 클리닝 실험 연구)

  • Lee, Hye-Youn;Cho, Nam-Chul;Lee, Jong-Myoung;Yu, Jae-Eun
    • Journal of Conservation Science
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    • v.27 no.3
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    • pp.301-312
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    • 2011
  • Epoxy resin has superior durability and adhesive strength and proper physical strength so that it is used to diversity materials for multi-purposes. However epoxy resin is hardly removed after hardening specially once it is applied to artefacts, it is difficult to remove them under re-conservation. This paper is an experimental study on removing epoxy resin applied to iron objects using Nd:YAG laser cleaning system. Tests conducted in this study investigated how increasing laser energy and pulses would give effect on samples. The samples were prepared in a way that epoxy resin, itself pure and one which was mixed with pigment and they were applied to iron coupons and corroded iron coupons respectively. As a result of experiment, pure epoxy resin applied to corroded iron coupons was ablated at high laser energy but epoxy resin applied to iron coupons and mixing with pigment were not ablated but discolored and bubbled due to laser-induced heat generation. Results of FT-IR showed no component alteration of shifted resins and no residues on the surfaces ablated by laser irradiation. From SEM-EDS for removed surfaces, the debris from epoxy resin and melting iron was observed. Therefore, this study demonstrated the possibilities and limitations for laser cleaning to remove epoxy resin from iron objects.

The Quantitative Effects of ERP Systems in Korean Manufacturing Industry (국내 제조기업의 ERP 시스템 도입의 정량적 성과에 관한 연구)

  • Chang, Hwal-Sik;Park, Kwang-Oh;Choi, Woo-Hyeok;Han, Jung-Hee
    • Management & Information Systems Review
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    • v.26
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    • pp.27-60
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    • 2008
  • Researches on the introduction of ERP system kept on examining the critical successful factors (CSFs) that focus on factors to achieve effectively successful projects, and trying to measuring the actual effectiveness of the introduction of ERP system. However, most of the preceding researches on the effectiveness of the introduction of ERP system that was searching devoted effects has been ceased, and actually even researches on the economical results have just done the basic cognitive evaluation of result indicators by many questionnaires instead of objective measuring values, because of the difficulty of measuring the evaluation of the result. Moreover, researches on positive effects of the introduction of ERP on enterprise results and researches that failed to give advantageous effects showed different results each other. And a part of researches reported that only a part of result indicators were partially affected. In this research, we investigated Korean large enterprises or middle-sized enterprises in manufacture industry that introduces SAP R/3 and Oracle package to compare their quantitative financial results after the introduction of ERP system, in order to measure the effects of the ERP system. First, we evaluated the difference of the quantitative financial results before and after the introduction of the ERP system. Second, we evaluated the opportunities shown by the effects after the introduction of the ERP system. Third, we removed the sample of the exchange crisis (IMF) and executed the additional analysis to reflect the average increasing and decreasing rate in the industry, so that pure evaluation can be achieved. Inherent limits of precedent researches are removed and practical effects of the pure introduction of the ERP system are evaluated, so the research of this research is significant. The result of this research is as follows. Because of the introduction of ERP, the rate of turnover of inventory property has increased and sales of preparation inventory property have decreased so that more effective inventory property management has been achieved. Moreover, preparation sales of labor costs and preparation sales of the number of employees have decreased to show the effect of the reduction of labor costs. However, it could no be concluded that we could increase the profit due to the introduction of ERP system. Due to the introduction of ERP, although we concluded that the return on assets (ROA) and the additional value of one-person employee statistically showed obvious differences and increased, the return on equity failed to show obvious differences after the process of introduction of ERP.

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The Effect of Hydrogen Reduction Treatment on Properties of Ba-ferrite (환원처리가 Ba-ferrite의 물성에 미치는 영향)

  • 홍양기;정홍식;김현준
    • Journal of the Korean Magnetics Society
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    • v.7 no.1
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    • pp.25-30
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    • 1997
  • Physical and magnetic behaviors of reduced Co-Zn-Ti-Sn substituted Ba-ferrite particles with hydrogen are different from those of reduced-pure Ba-ferrite particles. The coercivity of substituted Ba-feffite particles shows a peaking effect with the reduction temperature ranging from 250 to 520 $^{\circ}C$, while the coercivity of pure Ba-ferrite decreases monotonically. The reduction process of substituted Ba-ferrite has been found to be devided into three steps. At the first and second steps, the magneto-plumbite structure maintained. When the reduced-substituted Ba-ferrite particles are reoxidized, the coercivity is reversible at the first step but irreversible at the second step. During the third step of reduction process above 410 $^{\circ}C$. The magneto plumbite structure was collapsed with formation of $\alpha$-Fe and $BaFeO_{3-x}$ phases and consequently the coercivity distribution is broaden and the coercivity irreversible. The coercivity and saturation magnetization decreases and increases up to 130 emu/g respectively. In this study, it is found that the substituted elements prevent the magneto-plumbite structure from collapse during the reduction process and furthermore migrate from the magnetic sites of $2a+4f_{IV}$, 2b, and 12k to $4f_{VI}$ and 12k'. An increase in the coercivity before the collapse of magneto-plumbite structure is attributed to the migration of cations in hexagonal Ba-ferrite structure.

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Mechanical Properties of Natural Rubber/Acrylonitrile-Butadiene Rubber Blends and Their Adhesion Behavior with Steel Cords (Natural Rubber/Acrylonitrile-Butadiene Rubber 블렌드의 기계적 물성과 강선과의 접착거동)

  • Sohn, Bong-Young;Nah, Chong-Woon
    • Elastomers and Composites
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    • v.36 no.2
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    • pp.111-120
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    • 2001
  • Mechanical properties and their adhesion behavior with zinc- and brass-plated steel cords of natural rubber/acrylonitrile-butadiene blend compounds were investigated as a function of blend ratio. The Mooney viscosity and stress relaxation time were found to be lowered with increasing NBR content. Tensile modulus generally increased with increasing NBR content. Tensile stress at break stayed constant up to about 40 phr and showed minimum at $50{\sim}60 phr$, and thereafter increased with increasing NBR content. Strain at break decreased linearly below 50 phr, and above the level it showed nearly constant value. Based on the abrupt drops in elastic modulus and tan ${\delta}$ peak, the glass transition temperature of NR and NBR were found to be -55 and $-10^{\circ}C$, respectively. In the case of NR/NBR blend compounds, two distinct transition points were observed and each transition position was not affected by NBR level indicating an incompatible nature of NR/NBR blend system. The pullout force and rubber coverage decreased to the level of about 40% to that of pure m compound, when the 50 phr of NR was replaced by NBR. However, the pure NBR compound showed the comparable adhesion performance with NR(${\sim}90%$). The sulfur concentration was found to become lower with the increased NBR content at the adhesion interface based on the Auger spectrometer results, representing a lack of adhesion layer formation, and this was explained for a possible cause of low adhesion performance with adding NBR.

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Fabrication and Characterization of High Luminance WOLED Using Single Host and Three Color Dopants (단일 호스트와 3색 도펀트를 이용한 고휘도 백색 유기발광다이오드 제작과 특성 평가)

  • Kim, Min Young;Lee, Jun Ho;Jang, Ji Geun
    • Korean Journal of Materials Research
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    • v.26 no.3
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    • pp.117-122
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    • 2016
  • White organic light-emitting diodes with a structure of indium-tin-oxide [ITO]/N,N-diphenyl-N,N-bis-[4-(phenylm-tolvlamino)-phenyl]-biphenyl-4,4-diamine [DNTPD]/[2,3-f:2, 2-h]quinoxaline-2,3,6,7,10,11-hexacarbonitrile [HATCN]/1,1-bis(di-4-poly-aminophenyl) cyclo -hexane [TAPC]/emission layers doped with three color dopants/4,7-diphenyl-1,10-phenanthroline [Bphen]/$Cs_2CO_3$/Al were fabricated and evaluated. In the emission layer [EML], N,N-dicarbazolyl-3,5-benzene [mCP] was used as a single host and bis(2-phenyl quinolinato)-acetylacetonate iridium(III) [Ir(pq)2acac]/fac-tris(2-phenylpyridinato) iridium(III) $[Ir(ppy)_3]$/iridium(III) bis[(4,6-di-fluoropheny)-pyridinato-N,C2] picolinate [FIrpic] were used as red/green/blue dopants, respectively. The fabricated devices were divided into five types (D1, D2, D3, D4, D5) according to the structure of the emission layer. The electroluminescence spectra showed three peak emissions at the wavelengths of blue (472~473 nm), green (495~500 nm), and red (589~595 nm). Among the fabricated devices, the device of D1 doped in a mixed fashion with a single emission layer showed the highest values of luminance and quantum efficiency at the given voltage. However, the emission color of D1 was not pure white but orange, with Commission Internationale de L'Eclairage [CIE] coordinates of (x = 0.41~0.45, y = 0.41) depending on the applied voltages. On the other hand, device D5, with a double emission layer of $mCP:[Ir(pq)_2acac(3%)+Ir(ppy)_3(0.5%)]$/mCP:[FIrpic(10%)], showed a nearly pure white color with CIE coordinates of (x = 0.34~0.35, y = 0.35~0.37) under applied voltage in the range of 6~10 V. The luminance and quantum efficiency of D5 were $17,160cd/m^2$ and 3.8% at 10 V, respectively.

Molecular Docking Affinity Comparison of Curcumin and Nano-micelled Curcumin with Natural Sea Salt on Transthyretin (울금의 주요 성분인 커큐민과 나노 마이셀링 기법 적용 염화 커큐민의 트랜스타이레틴 활성 부위에 대한 결합 친화도 비교분석)

  • Kim, Dong-Chan;Song, Pyo
    • Journal of Life Science
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    • v.26 no.2
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    • pp.253-258
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    • 2016
  • In this study, nano-micelled curcumin was produced with natural sea salt with a view to comparing the in silico molecular binding affinity of pure curcumin compound to the active site of transthyretin. Using an optical light microscope and an electron microscope, it was found that the structure of the surface and the cross-section of nano-micelled curcumin was significantly different from natural sea salt. In particular, the crystal structure and nano-components in the nano-micelled curcumin were united, and the layer was more strongly stabilized than untreated salts. In the virtual 3D structure, in silico molecular docking study, the ligand binding affinity of nano-micelled curcumin to the transthyretin active site was found to be higher than that of pure curcumin. In addition, a nano-micelled curcumin formula interacted with more amino acid residues of transthyretin domains. The pharmacophore feature of the nano-micelled curcumin also showed more condensed and constrained features than normal curcumin. These results suggest that nano-micelled curcumin may effectively bind to and stabilize transthyretin, thereby regulating transthyretin-related physiological diseases. Collectively, the nano-micelled curcumin process suggests that normal curcumin can be modified more efficiently into the novel bio-functional chemical formula to stabilize the transthyretin structure. Therefore, the nano-micelled curcumin process can be applied to the field of the regulation of Alzheimer's disease.

Estimation of Ultimate Lateral Resistances of Piles Using CPT Cone Resistance in Sand (사질토지반에서 콘관입저항치 $q_c$에 의한 단말뚝의 극한수평단위지지력 평가)

  • Kim, Min-Kee;Kyung, Doo-Hyun;Hong, Jung-Moo;Lee, Jun-Hwan
    • Journal of the Korean Geotechnical Society
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    • v.24 no.11
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    • pp.71-77
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    • 2008
  • In this study, CPT-based methodology for estimating the ultimate lateral resistance, $p_u$, is proposed and verified for lateral loaded piles in sandy soil. Preexistent methods estimating the ultimate lateral resistance, $p_u$, and the ultimate lateral capacity, $H_u$, of pile have been based on the vertical effective stress, relative density, and the coefficient of lateral earth pressure. Similarly, cone resistance $q_c$ in pure sandy soil is expressed by those essential factors. As correlation between $p_u$ and $q_c$ are normalized with average effective stress ${\sigma}_m$, estimation methodology for the lateral loaded pile of $p_u$ in sandy soil is proposed. The method is verified by calibration chamber test results in pure sand. The standard derivation of estimated $p_u$ is 0.279, and COV (Coefficient Of Variation) of estimated $p_u$ is 0.272. These results showed that the estimated pus by the method are analogous with the measured $p_us$ in calibration chamber test.