• Korean Journal of Plant Taxonomy
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• v.41 no.4
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• pp.365-369
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• 2011

In this paper, a new hybrid species, Pulsatilla ${\times}$ yanbianensis H.Z. Lv, is named and described from the Yanbian area of Jilin Province in China. Molecular data confirm that this taxon is a result of natural hybridization between P. dahurica and P. cernua. As intermediate and mosaic phenotypes are expected for an interspecific hybrid, the leaves of P. ${\times}$ yanbianensis are fully expanded at the anthesis, resembling one of the parental species, P. daurica. However, the hybrid taxon is distinct from P. daurica by having reddish violet sepals and sterile fruits. Pulsatilla ${\times}$ yanbianensis also resembles the other parental species, P. cernua, in that it has dark red sepals and styles. The new hybrid, however, can be distinguished from P. cernua because it has leaf margins with short hairs and fully expanded leaves at the anthesis.

• Natural Product Sciences
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• v.19 no.2
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• pp.150-154
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• 2013

A new oleanane-type triterpenoid saponin together with six known saponins were isolated from the roots of Pulsatilla cernua. Their structures were elucidated on the basis of spectroscopic data, including 2D NMR spectra and chemical evidence. Compounds 1 and 6 are reported from this genus for the first time.

• Korean Journal of Pharmacognosy
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• v.42 no.1
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• pp.6-8
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• 2011

'BaegDuOng(白頭翁)' is one of the Chinese crude drugs used mainly as a pruritus, arthritis, cough and abdominal pain. The botanical origin of the crude drug has never been studied pharmacognostically. To clarify the botanical origin of 'BaegDuOng', the morphological and anatomical characteristics of Pulsatilla species growing in Korea, i.e. Pulsatilla koreana Nakai and P. cernua (Thunb.) Spreng. were studied. As a result, BaegDuOng from Korea was proved to be the root of Pulsatilla koreana.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.490-493
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• 2004

Karyotypes were established in five Pulsatilla species from Korea : P. cernua, P. davurica, P. koreana, P. chinensis and P. tongkangensis. The somatic chromosome numbers of five species were all 2n=2x=16 with the basic number of x=8. The chromosome complement of P. cernua consisted of 5 pairs of metacentric, 1 pair of submetacentric and 2 pairs of subtelocentric. P. davurica, P. koreana and P. chinensis consisted of 5 pairs of metacentric and 3 pairs of subtelocentric. P. tongkangensis consisted of 5 pairs of metacentric, 2 pairs of submetacentric, and 1 pair of subtelocentric. Karyotype formulas of P. davurica, P. koreana, and P. chinensis were the same as K (2n) = 2x = 16 = 10m + 6st, while those of P. cernua was K (2n) = 2x = 16 = 10m + 2sm + 4st and P. tongkangensis was K (2n) = 2x = 16 = 10m + 4sm + 2st, respectively.

• Archives of Pharmacal Research
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• v.12 no.1
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• pp.42-47
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• 1989

From the roots of Puisatiila koreana, three monodesmosides(pulsatilla saponins A, B and D) and two bisdesmosides(pulsatilla saponins F and H) were isolated. The structure of these saponins have been determined as hederagenin 3-O-${\beta}$-L-rhamnopyranosyl($1{\to}2$)- ${\alpha}$-L-arabinopyranoside(A), hederagenin 3-O-${\beta}$-D-glucopyrano syl($1{\to}4$) - ${\alpha}$-L-arabinopyranoside(B), hederagenin 3-O- ${\alpha}$-L-rhamnopyranosyl ($1{\to}2$)-[${\beta}$-D-glucopyranosyl($1{\to}4$]-${\alpha}$-L-arabinopyranoside(D), 3-O-${\alpha}$-L-rhamnopyranosyl($1{\to}2$)-{${\alpha}$-L-arabinopyranosyl hederagenin 28-O-${\alpha}$-L-rhamnopyrano syl($1{\to}4$)-${\beta}$-D-glucopyrano syl($1{\to}6$)-${\beta}$-D-glucopyranosyI ester (F) and 3-O-${\alpha}$-L-rhamnopyranosyl($1{\to}2$)-[${\beta}$-D-glucopyranosyl($1{\to}4$)]- ${\alpha}$-L-arabinopyranosyl hederagenin 28-O-${\alpha}$-L-lharnnopyranosyl($1{\to}4$)-${\beta}$-D-glucopyranosyl($1{\to}6$)-${\beta}$-D-glucop yranosyl ester(H) on the basis of chemical and spectral studies. Pulsatilla saponin B is the first report of its presence in plants but saponins A, D, F, and H have recently been isolated from the same genus p. cernua.

• The Korean Journal of Ecology
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• v.14 no.4
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• pp.379-398
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• 1991

In order to investigate the ecotypic variations of pulsatilla koreana nakai distributed in korea, 8 local populations of the plant were selected in accordance with latitude and compared on the differences of leaf shape, elongstion, stomatal distribution and dhlorophyll content. The incised angle of the plant is 38。32' in cheju, showing a great difference as compared with 23。77'-30。 32' of the other sites. as to the incised rate, mt. togyu and cheju representsed relatively high values of 49.04% and 48.21% the parted leaved individuals were only observed along the warm current coastal area including cheju, sokcho and sunchon and were 54(62.8%), 2(2.5%) and 3(3.3%) in the sites, respectively. Especially, the individuals consisting of only parted learves were 5(5.8%) in cheju alone and none of the other sites showed such individuals at all. So p. cernua distributed in cheju and p. koreana in inland area can be considered to beecotypic variations of the same species from the upper investigations. According to the elongations of leaf and rachis, the local populations could be classified into 3 types, such as warm current coastal area type(cheju,sunchon and sokcho),southern inland area type(andong, chungju and mt. togyu)and central inland ara type(mt. komdan and hongchon). The dentate numbera of 39.80-43.73 in the warm current area were also different from those of 87% in mt.togyu and 15% in hongchon, while opposite leaflets account for 100% in the other sites. The content of chlorophyll ranged from 0.609 mg/g. d.w.(cheju)to 0.924 mg/g.d.w.(hongchon), showing an increasing trends as the latitudes grow higher. The number of stomata on the low epidermis is much larger than that on the upper epidermis in the whole area. But both aspects showed an increasing tendency, too as the latitude becomes higher.

• Korean Journal of Plant Resources
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• v.21 no.5
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• pp.362-367
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• 2008

In order to investigate the effect of plant growth regulators on effective in vitro micropropagation, apical meristems of Pulsatilla cernua var. koreana were cultured on Murashige and Skoog's (MS) medium with 2,4-D, NAA, TDZ and BA. Media containing 2,4-D and kinetin, 2,4-D and TDZ, NAA and TDZ were not effective on callus induction. However, embryogenic or organogenic callus was obtained on media containing NAA and BA. Especially, on MS medium with 0.5mg/L NAA and 1.0mg/L BA was optimal for a high frequency (62%) of shoot or shoot bud obtained from callus. Callus proliferation, shoot multiplication and elongation were significantly increased by adding 10% coconut water on MS media with 0.5mg/L NAA and 1.0mg/L BA. Repeated subculturing of in vitro grown shoots resulted in propagation rate of 12.9 shoots per explant every 30 days. Root formation from the adventitious shoots was not easily achieved. However, roots were only produced through callus on MS medium with 2.0mg/L NAA alone or 0.5mg/L NAA and 1.0mg/L BA. These roots were used materials for callus and shoot production repetitively.