• 한방비만학회지
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• 제15권2호
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• pp.131-136
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• 2015

Objectives: The prevalence of obesity and metabolic syndrome is increasing worldwide. Regulation of cellular energy metabolis has the potential to be manipulated therapeutically to serve as a target for obesity and insulin resistance. Skeletal muscle is regarded as a target for regulation of energy metabolism and insulin resistance. In this study, the authors investigated the regulatory effect of (Cinnamomum cassia Blume, CCB), Aconitum carmichaeli Debx (ACD), and Benth (Pueraria lobata Benth, PLB) on energy and glucose metabolism in C2C12 myotubes. Methods: The water extracts of CCB, ACD, and PLB (0.5 mg/ml) were treated in differentiated C2C12 myotubes. The expressions of adenosine monophosphate-activated protein kinase (AMPK) and phosphorylation AMPK were detected with western blotting. Glucose metabolism was investigated with glucose uptake assay and glucose consumption assay, total adenosine triphosphate (ATP) content was also analyzed. Results: CCB, ACD, and PLB activated the phophorylation of AMPK, they also increased the glucose metabolism and total ATP contents in C2C12 myotubes. Conclusions: This study suggests that CCB, ACD, and PLB have the potential to increase energy and glucose metabolism in skeletal muscle.

• 한방비만학회지
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• 제17권2호
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• pp.119-126
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• 2017

Objectives: This study investigated the effects of the mixed herbal extract from Cinnamomum cassia Blume, Atractylodes macrocephala Koidzumi, Pueraria lobata Benth, and Aconitum carmichaeli Debx (CAPA) on obesity and glucose tolerance in obese mice. Methods: Animals were divided in 6 groups, normal diet, high fat diet (HFD), HFD with CAPA 100 mg/kg (CAPA 100), CAPA 300 mg/kg (CAPA 300), and metformin 200 mg/kg or lorcaserin 10 mg/kg as positive controls, and treated for 16 weeks. Body weight, food intake, fasting blood glucose, and body temperature were checked every week and then organs, blood serums were collected after treatment. The oral glucose tolerance test was also carried out after treatment. Results: Compared to HFD, CAPA extract treated mice showed significant decreases in body weight, adipose tissue weight, lipid accumulations in liver and serum lipid levels without a reduction of food intake. And fasting glucose and glucose tolerance were all improved in the CAPA treated mice. Conclusions: Our results suggest that CAPA extract can prevent diet induced obesity and glucose intolerance without a reduction of energy intake in obese mice.

• 혜화의학회지
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• 제13권2호
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• pp.179-186
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• 2004

Now many sustitution and false articles is used in korea instead of gegen. To use gegen correctly, we will make a quilitative certificational plan of gegen to investigate all of lieraturea, records and documents. And we could reach conclusions as folloews. 1) Source As a source of gegen, we think all of Pueraria lobata Ohwi and Pueraria thomsonii Benth can be used in korea and china. 2) Harvesting time. According to a data, content of effective components is high when harvested in January and February. Though there is no problem because domestic harvesting and working time is between January and February, we think it need to sure well-founded datas. 3) Standars of goods According to cutting methods, gegen divide into 'jiaogegen' and 'piangegen' from users So there is no problem at cutting methods because it is not a affective factor. 4) Processing A processing method is a best important case in express quility of herb. According to a data, effective components of peeled gegen is much less than that of non-peeled geeen. A tested samples is non-peeled gegen at a researh, they received suitable judgments in all of items. Especially we got a result content of puerarin is much more than 2.0%. But they were not washed in water, it is a problem that there is bleaching effect by almost drying at a briquat. So a processing method is to wash in water with unavoidable turnning to brown, to prevent this grgrn must be dried in bulk at low temporature. And we think that content of puerarin must be looked upward.