• Title/Summary/Keyword: Psychrotrophic bacterial counts. Coliform counts

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A Study on the Deterioration of Raw Milk Quality by the Growth of Psychrotrophic Bacteria (원유의 저온성 세균의 증식에 의한 유질변화에 관한연구)

  • 정충일;강국희;이재영
    • Journal of Food Hygiene and Safety
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    • v.1 no.2
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    • pp.151-156
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    • 1986
  • A trial was carried out to check the seasonal variation in total bacterial counts (T.B.C.) and psychrotrophic bacterial counts (P.B.C.) and to investigate the quality deterioration of raw milk collected from dairy farms in Kyunggi area, Korea. T.B.C. of raw milk exceeded $10^{7}/ml$ in summer and $10^{6}/ml$ in winter 1980, but they have gradually decreased from 1983. In 1985, the counts showed less than $2{\times}10^{6}/ml$ through the whole year. The same tendency of improvement in coliform counts was shown. The counts were higher than $10^{6}/ml$ in summer and $10^{5}/ml$ in winter 1982, but they were kept lower than $1{\times}10^{5}/ml$ in 1985 through the whole year. Free fatty acids and free amino acids were increased in raw milk stored at $5^{\circ}C\;and\;10^{\circ}C$, by the growth of psychrotrophic bacteria.

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The Conductance Determination of Total, Coliform and Psychrotrophic bacteria Counts in Raw Milk by Using Malthus (Malthus를 이용한 원유(原乳)내의 총균수, 대장균군수, 저온성균수 측정)

  • Nam, Eun-Sook;Chung, Choong-Il;Kang, Kook-Hee;Jeong, Dong-Kwan
    • Korean Journal of Food Science and Technology
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    • v.26 no.6
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    • pp.764-769
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    • 1994
  • This study was performed to obtain fast, consistant and reliable estimation system of bacterial counts of raw milk, which effectively related to the quality of sanitaion and the condition of production at the farm. This study compared regression equation and correlation coefficient relationship between standard plate counts and data of Malthus conductance method for the detection time of total, psychrotrophs, coliform bacterial counts in raw milk. Regression equation (RE) between conductance detection time (Y) and total bacterial log counts (X) was Y=18.27651 - 2.07550X, with correlation coefficient -0.95(n=201). In coliform, RE was Y=9.320848 - 1.15598X with correlation coefficient -0.90 (n=207). Psychrotrophs had the RE of Y=29.96008-3.02487 with correlation coeffecient -0.9 (n=201). This conductance method gave results more quickly and was less labor-intensive than traditional standard plate count method.

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A STUDY ON PSYCHROTROPHIC COLIFORM AND BACTERIAL FLORA IN SHELLFISH (패류중의 저온성 대장균군 및 세균 Flora에 관한 연구)

  • SHIH Suk U;KANG Sung Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.12 no.1
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    • pp.19-26
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    • 1979
  • To evaluate the sanitary conditions of four species of commercial shellfish (Crassostrea gigas, Tapes japonica, Mytilus coruscus and Anadara granosa), the psychrotrophic coliforms and the bacterial flora were monthly examined from January to December, 1978. Viable cell counts of the samples submitted were $1.3\times10^3\~2.5\times10^6/g$ and the numbers of coliform and E. coli were above $0\~5.5\times10^2/g\;and\;2\~9.2\times10^2/100g$ respectively. Among the 373 coliform bacteria isolated from the samples submitted, 298 strains were identified as coliform by IMViC test, $44^{\circ}C$ culture and gelatin liquefaction test. E. coli type I and K. aerogenes type I were the most predominant ones among the identified coliform as 138 strains and 71 strains respectively. The psychrotrophic coliform growing at $5^{\circ}C$ were 186 $strains(50\%)$ among 373 strains of isolated coliform. Vibrio and Pseudomonas were the most predominant groups. Among 453 strains of isolated bacteria, Vibrio was 240 $strains(53\%)$ and Pseudomonas was 91 $strains(20\%)$. Moraxella, Acinetobacter, Enterobacteriaceae and Flavobacterium-Cytophage were scantly detected. In seasonal variation of general bacterial flora, a lot of Vibrio appeared from May to October, did Moraxella during the winter season and the other genuses showed no seasonal change.

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Comparison of the Keeping Quality of UHT Pasteurized Milks in Korea (국내 시판 우유의 보존성 비교)

  • Chung, Chung-Il;Kim, Kwag-Tae;Cho, Nam-Yong;Jung, Min-Jung;Oh, Hyun-Suk;Lee, Gil
    • Food Science of Animal Resources
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    • v.22 no.3
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    • pp.247-251
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    • 2002
  • This study was carried out to investigate the keeping quality of market milks collected from several dairy processing companies. Microbiological, chemical, and sensory properties of Intra-high temperature(UHT) treated milks stored at 5, 10, 15t were evaluated during the storage of 154days. No significant difference during the storage was shown in bacterial counts, pH and titratable acidity. Standard plate count(SPC) was remained less than 20,000 cfu/ml after 15 day storage at 5, 10, 15$^{\circ}C$. Also, Psychrotrophic bacterial count and Coliform count were not detected or detected at low level. The pH ranged from 6.55 to 6.83 and the titratable acidity ranged from 0.13% to 0.16%. In sensory evaluation, UHT milks showed normal flavor until 10days, but thereafter off-flavor was slightly developed. Consequently, UHT milks remained in good quality when stored at 5$^{\circ}C$,10$^{\circ}C$t until 15days except sensory properties. According to this experiment, the shelf-life of UHT milk can be extended to 10 days, even though maintaining the storage temperature strictly below 10 t in milk market is not easy.