• 한국환경보건학회지
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• 제42권2호
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• pp.92-101
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• 2016

Objectives: Outdoor workers are exposed to thermally stressful work environments. In this study, heat stress indices for harbor workers in summer were calculated to evaluate thermal comfort based on a human heat balance model. These indices are Physiological Subjective Temperature (PST), Dehydration Risk (DhR), and Overheating Risk (OhR) according to respective stage of cargo work in a harbor. In addition, we constructed a forecast system to provide heat stress information. Methods: Thermophysiological indices in this study were calculated using the MENEX model (i.e. the human heat balance model), which used as inputs the meteorological parameters, clothing insulation, and metabolic rate for each stage of cargo work in the harbor of Masan over the course of seven days, including a four-day heat wave. The forecast heat stress information constructed for Masan harbor was based on meteorological data supported by the Dong-Nae Forecast from the KMA (Korea Metrological Administration) and other input parameters. Results: According to higher metabolic rate, thermophysiological indices showed a critical level. In particular, PST was evaluated as reaching the 'Very hot' or 'Hot' level during all seven days, despite the heat occurring over only four. It is important in a regard to consider the work environment conditions (i.e. labor intensity and clothing in harbor). On a webpage, the forecast thermophysiological indices show as infographics to be easily understand. This webpage is comprised of indices for both current conditions and the forecast, with brief guidance. Conclusion: Thermophysiological indices show the risk level to health during a heat wave period. Heat stress information could help to protect the health of harbor workers. Further, this study could extend the applicability of these indices to a variety of outdoor workers in consideration of work environments.

• 한국미생물·생명공학회지
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• 제24권4호
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• pp.415-422
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• 1996

A gene coding for a pyruvyl transferase enzyme involved in exopolysaccharide biosynthesis of Zoogloea ramigera 115SLR was isolated and sequenced. A 4.5 kb of BamHI DNA fragment was isolated from chromosomal DNA using a probe derived from ketal pyruvyl transferase gene of Xanthomonas campestris. The nucleotide sequence of 2.66 kb Pst1/HindIII DNA fragment which was homology with a probe revealed the existence of two complete open reading frames (ORF2 and ORF3) and two partial open reading frames (ORFI and ORF4). The deduced amino acid sequence of ORF3 was homologous to the ketalase (GumL product) of X campestris with 49.5% of similarity and 21.6% of identity. ORF2 on the other hand showed the higher identity with the ketalase (ExoV product) of Rhizobium meliloti (36%) as well as the ketalase of X campestris (23%) than that of ORF3. A gene product of ORF2 was determined with a bacteriophage T7 RNA polymerase/promoter system in E. coli. The molecular weight of protein was 33,500 dalton.

• 한국연초학회지
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• 제13권2호
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• pp.34-41
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• 1991

To explore the possibility of introducing Zea mays transposable element Ac(activator) which can be used as a mutagen and gene tag in tobacco plants other than maitre, we tried to introduce a cloned Ac element into tobacco cells by an Agrobacterium tumefaciens binary vector system. Transformation of N. babacum cv. Burley 21 tissues and regeneration to whole plant were carried out. The frequency of the transformed callus induced in shoot induction media was higher than that of transformed callus induced in callus induction media. However, the calli were not grown in the second selection media, and became yellow senescent calli. Regenerated tobacco plantlets with foreign gene were also obtained in shoot induction media containing 100 $\mu\textrm{g}$/ml kanamycin and 100$\mu\textrm{g}$/ml carbenicillin. The leaf tissues of transformant was also resistant to 1000 $\mu\textrm{g}$/ml kanamycin. The chromosomal DNAs of transformant and normal plant of N. tabacum were digested by EcoR I and Hind III but not by Pst I.

• 미생물학회지
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• 제29권2호
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• pp.117-122
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• 1991

In order to improve the removal ability of phosphate, Spheroplast fusions were performed among auxotrophic mutants of Aeromonas hydrophila isolated from waste water, named A13 and A14, Aci37 auxotrophic mutant of Acinetobactercalcoaceticus, and auxotrophic E. coli HR262/pCE27 carring pit gene. Eight fusants obtained from this experiment showed different biochemical characteristics. When the rate of phosphate uptake among fusants (F1-F8) was investigated in Phosphate Uptake Medium (PUM), F8 strain showed the highest rate for phosphate removal, 7 times as much as control after two hours incubation. The role of cations ($Mg^{++}$ ,$Ca^{++}$ , $K^{+}$ in phosphate uptade by F8 was also investigated in PUM without each salt. $K^{+}$ seemed to be crucial. Being compared with phosphate untake rate in PUM, that in PUM without $K^{+}$ was reduced 1.5 times. Therefore, by applying F8 strain and $K^{+}$ in practical environmental system, the increased efficiency in phosphate removal can be derived.

• The Korean Journal of Ceramics
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• 제1권2호
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• pp.81-85
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• 1995

PST-series spcimens with stoichimetric and nonstoichimtric compositins were prepared and the effects of compositionl modification on phase formation and dielectric presponse were investigated. The phases formed on calcination were mainly perovskite and trace amount of phyrochlore(s), with an increase of the latter phase(s) as the composition became more ononstoichiometric. The sintered samples showed thermal hysteresis and diffuseness in phase transition with a small degree of frequency relaxation. Temperatures corresponding to maximum values of dielectric constant and loss were relatively insensitive to the composition change while the maximum values were very sensitive to that.

• 복식문화연구
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• 제9권1호
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• pp.45-60
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• 2001

The purpose of this research is to offer basic data for the study of men's suit by investigating purchase behavior and fit of men's ready-made suit for Koran adult men. The subjects were male who aged 25∼39 and resided in Seoul, Kyunggi and Chungnam regions. The results of the research are summarized as the following : 1. Male consumers purchase suit most frequency at department, specially stores. As the consumers grow older dependency on their spouses'opinion for purchase decision increases. The standards of purchasing are stated to e design, price and color. 2. the male consumers are generally knowledgeable about the current sizing system. Yet, the knowledge is confused with the pst and present systems. 3. When the male consumers purchase, they consider mainly for the fit of th d\\body part are shoulders for the jacket and waist, hip for the pants. 4. The attitude to toward the combination of sizes for the jacket is comparatively satisfaction than pants. Specially, most negative part of jacket is the length of sleeve.

• 대한수의학회지
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• 제47권1호
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• pp.59-65
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• 2007

Pasteurella multocida Pasteurella multocida toxin (PMT) is a causal pathogenin atrophic rhinitis in pigs. To investigate the protective immunity and vaccination effect of recombinantPMT, the gene for PMT was isolated from the infective P. multocida D:4. The 2.3 kb XhoI/PstI fragment(PMT2.3) of PMT gene was expressed in E. coli BL21 (DE3) using the induced expression vector system.The recombinant protein of PMT2.3 having molecular weight of 84 kDa was purified by Ni-afinitycolumn chromatography. The PMT2.3 raised slightly less anti-PMT antibody titer than formalin-killedwhole cel, however, it showed more protective imunity against P. multocida D:4 infection in vaccinationand chalenge.

• 미생물학회지
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• 제22권4호
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• pp.249-255
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• 1984

To develop the host-vector system for industrial Coryneform bacteria that seemed to be the most suitable microorganisms for molecular breeding of genes involved in the production of amion acids, nucleotides, and other products of industrial interest, broad host range E. coli plasmid R 1162 DNA was transformed into Brevibacterium ammoniagenes and the plasmids pKU6 isolated from a transformant was physically characterized. All other plasmids from the transformed cells except pKU6 exsisted as multimeric forms in Brevibacterium ammoniagenes. The plasmid DNA was retransformed into Corynebacterium glutamicum with a high frequency ($1.32{\times}10^{-1}$ per cell) and maintained stably both in Brevibacterium ammoniagenes and Corynebacterium glutamicum after 100 generations of cultures with 25-30 copy number per cell. The size of both plasmid pKU6 and plasmid R1162 were the same and restriction maps by EcoR I, Ava I, Pst I, Pvu II and Hinc II were also similar.

• 한국실내디자인학회논문집
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• 제28호
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• pp.158-166
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• 2001

e-revolution makes a lot of changes in the methodology all over the world. That is, the theory of real time showing helps people to access audio and visual wherever and whenever they are. In the pst computers were considered as only tools which could make us work easily. However, the meaning of computer is changing with e-revolution nowadays. Computers are not just computers as they were; they have done a lot of things which we thought impossible and they will do in the future as well. This new wave encourages people who are teaching the design to use computers whatever they do. For example, instead of using pencil and a drafting board, most people in the design field work with monitors, mouse and plotter. Therefore, most people who are in the design field need to have the ability of computer skills. They have to use computers not only in their class but also in their office. However, if we use computers for visual presenting in the class, it will not be enough to catch the e-revolution. That is, we should work with computers in the creative and conceptual design such as the using of the design information and the applying digital techniques in the early stage of the work. The purpose of this study is to show how to work with computers in the spacial design process especially th using of the DIS(Design Information System) and the applying digital techniques in the early stage of the work.

• 조명전기설비학회논문지
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• 제16권2호
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• pp.45-53
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• 2002

본 논문은 두 지역 간에 직렬로 연결된 HVDC 연계시스템의 전력계통 안정화장치와 전력변환기의 적정 파라메터 선정기법을 다루었다. PSS파라메터 선정기법은 구하고자 하는 진상이 되도록 극좌표를 이동시키고, 충분한 댐핑을 얻도록 이득을 조절하는 고전적인 기법이다. 전력변환기의 적정 파라메터는 근궤적기법을 근거로 하여 기준 값의 변화와 시스템의 동요에 충분한 속응력과 안정도를 고려하여 산정한다. 이들 기법으로 구판 제정수로 소신호 및 과도안정도 분석결과는 사례연구시스템의 고유동요 주파수에 적절한 댐핑 효과를 보여주었다. 본 논문에서 사용한 프로그램은 MATLAB을 근거로 한 PST이다.