• The Korean Journal of Mycology
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• v.40 no.3
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• pp.174-176
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• 2012

We report morphological and physiological characteristics of newly isolated six kinds of yeasts which are new in Korean mycoflora. The yeasts were isolated from wild flowers in Daejeon city, Korea and identified by molecular analysis of the amplified internal transcribed space rDNA sequence or partial 18S rDNA sequence. Species of isolates were identified as Kuraishia capsulate, Lodderomyces elongisporus, Pseudozyma antarctica, Starmerella bombicola.

• Korean Journal of Microbiology
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• v.51 no.3
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• pp.187-193
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• 2015

Candida (also known as Pseudozyma) antarctica lipase B (CAL-B) has been intensely studied in academic and industrial fields. However, the research related to its homologous enzymes has been rarely reported. In the current investigation, protein sequence similarity search of CAL-B has been conducted and six homologous protein sequences were identified. After the syntheses of their codon-optimized genes, the synthetic genes have been cloned into a periplasmic expression vector to express in Escherichia coli. Among six homologous sequences, four sequences were successfully expressed in E. coli. The hydrolytic activities of the expressed proteins towards 4-nitrophenyl acetate and 4-nitrophenyl butyrate were measured and compared with those of CAL-B to identify whether the expressed proteins work as a hydrolase. It has been revealed that the expressed proteins can hydrolyze the substrates and the specific activities were determined as $(1.3-30){\times}10^{-2}{\mu}mol/min/mg$, which are lower than those of CAL-B. Among these homologous enzymes, Pseudozyma hubeiensis SY62 exhibits the comparable enantioselectivity to that of CAL-B towards the hydrolysis of (${\pm}$)-1-phenylethyl acetate.

• The Korean Journal of Mycology
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• v.43 no.1
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• pp.64-67
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• 2015

Several kinds of wild yeasts were isolated and identified from some cereals. A total of twenty six yeast strains were isolated from eleven kinds of cereals. Among twenty six yeast strains, Saccharomyces cerevisiae were five strains and Pseudozyma antarctica were four strains. Five species of Cryptococcus including Cryptococcus magnus were also isolated. Pseudizyma aphidis were isolated from black bean, and Saccharomyces cerevisiae, Cryptococcus flavescens, Cryptococcus magnus and Hannaella zeae were also isolated from glutinous millet.