• Title/Summary/Keyword: Pseudomonas putida.

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Production of Indigoid Pigments by Persolvent Fermentation with Pseudomonas putida BCNU 106 (Pseudomonas sp. BCNU 106의 persolvent fermentation에 의한 인디고이드계 색소 생산)

  • Choi, Hye Jung;Kwon, Gi-Seok;Joo, Woo Hong
    • Journal of Life Science
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    • v.24 no.1
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    • pp.81-85
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    • 2014

  • Pseudomonas sp. BCNU 106 isolated from industrial wastewater was able to produce indigo from indole by utilizing various organic solvents. BCNU 106 produced indigo effectively when grown in the presence of a large volume of p-xylene, propylbenzene, and mesitylene and a high level of indole. The present study demonstrated that the maximal yield was achieved with 20% (v/w) p-xylene and 4 g/l indole. Under these conditions, the indigo yield and the transformation efficiency of indole were 315.5 mg/l and 97%, respectively. The results suggest that Pseudomonas sp. BCNU 106 might be a potential candidate for industrially important indigo production.

  • https://doi.org/10.5352/JLS.2014.24.1.81 인용 PDF KSCI KPUBS HTML

Isolation and Identification of a Histamine-degrading Barteria from Salted Mackerel (자반고등어에서 histamine 분해능을 가진 세균의 분리 동정)

  • Hwang Su-Jung;Kim Young-Man
    • Journal of Life Science
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    • v.15 no.5 s.72
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    • pp.743-748
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    • 2005

  • Histamine can be produced at early spoilage stage through decarboxylation of histidine in red-flesh fish by Proteus morganii, Hafnia alvei or Klebsiella pneumoniae. Allergic food poisoning is resulted from the histamine produced when the freshness of Mackerel degrades. Conversely it has been reported that there are bacteria which decompose histamine at the later stage. We isolated histamine decomposers from salted mackerel and studied the characteristics to help establish hygienic measure to prevent outbreak of salted mackerel food poisoning. All the samples were purchased through local supermarket. Histamine decomposers were isolated using restriction medium using histamine 10 species were selected. Identification of these isolates were carried out by the comparison of 16S rDNA partial sequence; as a result, we identified Pseudomonas putida strain RA2 and Halomonas marina, Uncultured Arctic sea ice bacterium clone ARKXV1/2-136, Halomonas venusta, Psychrobacter sp. HS5323, Pseudomonas putida KT2440, Rhodococcus erythropolis, Klebsiella terrigena (Raoultella terrigena), Alteromonadaceae bacterium T1, Shewanella massilia with homology of $100\%,{\;}100\%,{\;}99\%,{\;}99\%,{\;}99\%,{\;}99\%,{\;}100\%,{\;}95\%,{\;}99\%,{\;}and{\;}100\%$respectively. Turbidometry determination method and enzymic method were employed to determine the ability of histamine decomposition. Among those species Shewanella massilia showed the highest in ability of histamine decomposition. From these results we confirmed various histamine decomposer were present in salted mackerel product in the market.

  • https://doi.org/10.5352/JLS.2005.15.5.743 인용 PDF KSCI

Diversity and Phylogenetic Analysis of Fluorescent Pseudomonads Isolated from Soil-Root System of Red Pepper in Greenhouse (비닐하우스 고추재배지의 토양과 근계로부터 분리된 형광성 Pseudomonads의 계통 분류 및 다양성)

  • Kwon, Soon-Wo;Kim, Jong-Shik;Song, Jae-Kyeong;Ryu, Jin-Chang
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.4
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    • pp.275-282
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    • 2000

  • Among the fluorescent pseudomonad isolates from soil- root system of red pepper in Chinju, Kyunsangnam-Do, the phylogenetic analysis for 35 isolates were conducted. The partial 16S ribosomal DNA sequences were used as taxonomic key for phylogenetic analyses, and these sequences were enabled to identification of the fluorescent pseudomonad isolates on the species level. The 17 isolates among them were classified into Pseudomonas putida group, and consisted of the strains isolated mainly from soil. This group were subdivided into 4 subgroups (I, II, III, and IV). The subgroup I and IV were unique ones which were relatively remotely related with subgroup II and III including the type strain of P. putida. The 15 isolates among 35 isolates were grouped along with the type strain of Pseudomonas fluorescens, and 3 isolate were characterized as intermediates of P. fluorescens and Pseudomonas chlororaphis. Most of strain isolateds from the rhizosphere soil and rhizoplane of red pepper were identified as P. fluorescens and closely related with each other. In this study, root of red pepper was supposed to be colonized by a specific strain or strains of P. fluorescens.

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