• Journal of Environmental Science International
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• v.6 no.2
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• pp.173-182
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• 1997

The bacterial strains, which utilizes 2,4,4'-trichloro-2'-hydroxydiphenyl ether(TCHDPE) as a sole carbon source, were isolated by selective enrichment culture from soil samples of industrial waste deposits. The bacterium that showed the highestt biodegradation activity was designated as EL-O47R The isolated strain EL-O47R was Identified as the genus Pseudomonas from the results of morphological, cultural, and biochemical tests. The optimum conditions of medium for the growth and the degradation of TCHDPE were TCHDPE 500 ppm, (NH4)2SO4 0.1% as the nitrogen source, initial pH 7.0±0.1, and 37℃, respectively. In this conditions, the regradation rate of TCHDPE was about 97%. Pseudomonas sp. EL-O47R was tested for resistance to several metal compounds and antibiotics. Pseudomonas sp. EL-O47R was moderately grown to Cd(NO3)2, ZnCl2, AgSO4, CuSO4 and HgCl2. This strain was sensitive to rifampicin and kanamycln but resistant to ampicillin, penicillin, tetracyclin and chloramphenlcol. Pseudomonas sp. EL-O47R was grown structurally related com- pounds and potential metabolites of TCHDPE, and has the stability on TCHDPE biodegradation.

• Microbiology and Biotechnology Letters
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• v.26 no.4
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• pp.303-308
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• 1998

A bacterial strain which utilizes phenol under denitrifying condition was isolated from the industrial waste water collected from the Chong-ju Industrial Complex. The strain was identified as Pseudomonas species from the morphological, physiological, and biochemical characteristics and designated as HL100. The strain can utilize phenol as the sole source of carbon and energy when nitrate is provided as the terminal electron acceptor. The isolated strain completely degraded 3 mM of phenol within 110 hour with concomitant reduction of nitrate to nitrite. The observed maximum doubling time was 20 hours. Under appropriate condition, complete reduction of nitrate to atmospheric N$_2$ was observed indicating that the isolated strain could perform complete steps of denitrification. The strain showed optimal growth at pH 7.0 and temperature of 37$^{\circ}C$ under denitrifying phenol-degrading condition. The strain can also utilize toluene as the sole carbon and energy source under the same growth condition. However, no growth was detected on xylene and benzene.

• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.14-19
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• 1992

The xylanase producing microorganisms occurring on rotten woods were selectively isolated on the modified Czapek-Dox medium supplemented with 0.5% xylan as a sole carbon source. Among more than three-hundred isolates of xylanase producing microorganisms, only two bacterial isolates were turned out to be more potent xylanase producer than the reference strain of xylanase producer, Aureobaszdium pullulans NRRL Y-2311. The exo-xylanase producer, bacterial isolate No. 33 was identified as a strain of Pseudomonas sp. on the basis of morphological and biochemical characterizations as well as cellular fatty acid composition. Optima of pH and of temperature for enzyme reactions of xylanase were 5.5 and $50^{\circ}C$ respectively. The enzyme was stable in a range of pH 5.0~7.0 and below $45^{\circ}C$. Among the number of carbohydrate substrates, xylose was turned out to be a potent inducer of Pseudomonas sp. No.33 exo-xylanase. Among the raw materials tested, rice straw was the best material for xylanase production by Pseudomonas sp. strain No. 33.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.428-433
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• 1993

A strain that inhibited the growth of Pellicularia sasakii was isolated from the soil and identified as Pseudomonas aeruginosa 3120. A dark brownish antibiotic, MRL3120 isolated and purified from the culture broth of P. aeruginosa 3120 was soluble in ethylacetate, chloroform and methanol, and it was active against gram-positive and negative bacteria as well as fungi. The structure of MRL3120 was identified as a chelate compound consisting of two N-methyl-N-thioformyl-hydroxylamine and a copper ion by the analysis of UV, IR, and EI-MS spectra and other physico-chemical properties and supposed to have a structure of fluopsin C related compound. Addition of $CuSO_4$ into the fermentation medium containing soybean meal increased antifungal activity but no activity was found in the presence of EDTA (0.1%, v/v). However antibiotic MRL3120 was not produced in the fermentation medium containing soytone instead of soybean meal but it was rapidly produced by the addition of $CuSO_4$.

• Korean Journal of Microbiology
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• v.32 no.1
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• pp.60-64
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• 1994

Pseudomonas syringae pv. tabaci produces tabtoxin and causes wildfire disease on tabacco and bean plants. In this study, bacteriophage of P. syringae pv. tabaci were isolated from sewage by top agar overlay method, and physiological and genetical characteristics of the phage were investigated. Plaques of isolated phage were turbid and ranged in size from 1 to 2 mm. The stability range of pH was between 6.0 and 9.0, and stability of temperature was up to 30${\circ}C$ and inactivated at 70${\circ}C$. The adsorption rate of phage was about 85% for 30min. The latent period and mean burst size as dertermined in one step growth experiments were 3 hrs and 200 PFU/bacterium, respectively. Genomic material of isolated phage was dsDNA of which size was about 30kb.

• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.124-130
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• 1998

An alkalophilic bacterium, the strain AC-711 as a potent producer of alkaline cellulase, was selected among many isolates from soil environments. Morphological, physiological and chemical characteristics of the strain AC-711 suggested that it belongs to the genus Pseudomonas according to the Bergey's Manual of Systematic Bacteriology, however the G+C mol% (54.43) of its chromosomal DNA is lower than the normal values of the genus. The major cell wall fatty acids were determined as 15:0 and 17:0 anteiso. The production of alkaline CMCase by the strain was maximal when grown on the mediun containing 1% carboxymethyl cellulose, 0.1% $KH_2PO_4$, 0.02% $CoCl_2$, 0.02% Tween 80, 0.5% $Na_2CO_3$, 0.8% yeast extract, pH 10.3 at $30^{\circ}C$ for 3 days, and the most of enzyme was excreted into culture mediun.

• Korean Journal of Microbiology
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• v.30 no.1
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• pp.47-52
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• 1992

This study describes isolation and identification of a soil bacterium which is degradable of phosphinothricin and improvement of the isolated strain by using mutagenesis and spheroplast fusion. The experiment was performed to search for a possibility of development of a new strain which is both PPT-degradable and glyphosate-resistant by using interspecies cell fusion between the PPT-degrading bacterium. Pseudornonu.\ puucimohlis and a glyphosate -resistant strain, Pseudornonu.~ cc,pucicl. Auxotrophic mutants were obtained by the treatement of P. puucimohili.\ with ethylmethanosulfate, and used to cell fusion. Lysozyme and EDTA were used to spheroplast formation and regeneration rates :)f the spheroplast were 6.5'%1 in P. pauc.irnohili.\ and 8.8% in P.ci,j~u[,i(lr, espctively. Polyethylenglycol 5.000 was used to cell fusion as fusogen. The fusant\ulcorner F1. F2. F\ulcorner and F4 werc- obtained by the intra- and interspecies cell fusion. The fusant Fl of intraspecies cell fusion was higher to the wild type by 1 I'%l in PPT degrading ability, and the fusant F3 of inierspesis cell fusion developed plyphosatc-resistant and PPI-dcgrading ability which were propertics of two parental strains.

• KSBB Journal
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• v.15 no.1
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• pp.27-34
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• 2000

A marine bacterium having a high oil-degrading activity was isolated form the oil-polluted southern sea of Korea, and was identified as Pseudomonas aeruginosa and was named Pseudomonas aeruginosa BYK-2. The optimal tmeperatur, culture time, pH and NaCl concentration for biosurfactant production and cell growth showed $25^{\circ}C$, 48h, 7.0 and 0%(w/v), respectively. After cultivation at $25^{\circ}C$, 180 rpm in 250 mL erlenmeyer flask for 7days, 1%(w/v) arabian light crude oil and bunker C oil which are considered to be hardly degradable compounds were degraded 92.1%(w/w) and 76%(w/w) respectively. And then, cell adherence was measured on various carbon sources. The cell adherence indicated over 80% on hydrocarbons(arabian light crude oil, kuwait curde oil, bunker C oil, n-paraffine, n-hexadecane, n-tetradecane) as carbon sources. Lecithin among fatty acids(oleic acid, olive oil, lecithin) showed highest cell adherence of 91.5%. The cell adherence of sugars(arabinose, trehalose, dextrose, galactose, lactose, fructose, maltose, sorbitol, sucrose) observed to be less than 70% except for arabinose, galactose, sorbitol and sucrose.

• Journal of Food Hygiene and Safety
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• v.37 no.2
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• pp.55-62
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• 2022

The bacterial soft-rot disease is one of the most critical diseases in vegetables such as Chinese cabbage. The researchers isolated two bacteria (Pseudomonas kribbensis and Pantoea vagans) from diseased tissue samples of Chinese cabbages and confirmed them as being the strains that cause soft-rot disease. Lactic-acid bacteria (LAB), were screened and used to control soft-rot disease bacteria. The researchers tested the treatments with hypochlorous acid water (HAW) and LAB supernatant to control soft-rot disease bacteria. The tests confirmed that treatments with the HAW (over 120 ppm) or LAB (Lactobacillus plantarum PL203) culture supernatants (0.5 mL) completely controlled both P. kribbensis and P. vagans.

• Fisheries and Aquatic Sciences
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• v.9 no.2
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• pp.57-63
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• 2006

A bacterial strain capable of hydrolyzing carrageenan was isolated from the coast of Busan in Korea. The isolated strain (HS5322) is aerobic, gram-negative, rod-shaped, and motile. Comparison of the 16S rDNA of the isolate with that of known Pseudomonas sp. showed that sequence similarity was at most 95%, implying that the isolate is a new Pseudomonas species. The organism grew optimally at NaCl concentrations of 2.0 to 2.5%. The optimum temperature and pH for carrageenase production in a 72-h flask culture containing 1% carrageenan was $20^{\circ}C$ and pH 8.5, respectively. Of the synthetic substrates tested, the highest enzyme activity was obtained with p-nitrophenyl ${\beta}$-D-galactopyranoside.