• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.626-629
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• 2000

The Pseudomonas aeruginosa BYK-2(KCTC 18012p) produced three kinds of glycolipids on olive oil as a substrate and purified two types of major glycolipids(Rf=0.48, BS-1; Rf=0.65, BS-2) using silica gel chromatography, TLC, HPLC, etc. From the analysis of the chemical structure, the glycolipid of BS-1 was estimated as rhamnolipid($2-O-{\alpha}-L-rhamnopyranosyl- {\alpha}-L-rhamnopyranosyl-{\beta}-hydroxyldecanoyl-{\beta}-hydroxydecanoic$ acid; M.W. 650) and BS-2 was detected as rhamnolipid methyl ester($2-O-{\alpha}-L-rhamnopyranosyl-{\alpha}-L-rhamnopyranosyl-{\beta}-hydroxyldecanoyl-{\beta}-hydroxydecanoic$ acid methyl ester; M.W. 664) by FT-IR, FAB Mass spectrometry, $^1H-NMR$, $^{13}C$ FT-NMR, DEPT, 2D-NMR (TOCSY, RELAY, NOESY, HSQC, HMBC). In particular, It was found that a marine bacterium Pseudomonas aeruginosa BYK-2(KCTC 18012P) remarkably produced rhamnolipid and rhamnolipid methyl ester simultaneously.

• 한국환경보건학회지
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• 제22권3호
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• pp.98-102
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• 1996

This study investigated performance of the phenol degradation and reaction characteristics according to variation of phenol volumetric loading rates and dilution rates in suspension and PACT reactors using Pseudomonas sp. B3. 1. Removal efficiencies of the PAC unit indicated about 100 % with phenol volumetric loading rates from 0.4 phenol $kg/ma^3\cdot d$ to 1.2 phenol $kg/m^3\cdot d$, however, which of the suspension reactor showed about 100% with from 0.2 phenol $kg/m^3\cdot d$ to 0.75 phenol $kg/ma^3\cdot day$. 2. The cell density slightly was decreased from 298.2 mg/l to 272 mg/l, when dilution rate for suspension was reactor increased from 0.4 to 1.41 1/d, and also the cell density suddenly was decreased to 145.5 mg/l and was washed out at the dilution rate higher than 1.60 1/d. But the cell density for the PAC unit was linearly decreased with dilution rate of from 0.8 to 3.0 1/d, and showed 220.75 mg/l at maximum dilution rate. 3. The phenol utilization rate was increased from 0.008 to 0.031 phenol g/l$\cdot$h, when dilution rate for suspension reactor was increased from 0.4 to 1.5 1/d, however, the rate for the PAC unit was linearly increased from 0.017 to 0.061 phenol g/l$\cdot$h as variation changes from 0.017 to 0.061 phenol g/l$\cdot$h dilution rate.

• 대한화학회지
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• 제39권6호
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• pp.453-458
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• 1995

Pb^{2+}$을 Pseudomonas alcaligenes 5S rRNA의 구조 분석에 응용하였다. Pb^{2+}$이 5S rRNA를 절단하는 방식은 5S rRNA의 삼차구조의 조사에 이용할 수 있을 것으로 기대되는 몇 가지 특징들을 보였다. Pb^{2+}$은 안정한 나선형 줄기들에는 작용하지 않는다. 단일가닥으로된 구역들 또는 내밀린 부분들은 그들의 분자내에서의 위치에 따라 다른 민감도로 작용을 받는다. 불안정한 d 나선은 전혀 작용을 받지 않는다. 불안정한 C 줄기에서는 3'쪽의 가닥만이 작용을 받고 5'쪽의 가닥은 작용을 받지 않는다. Pb^{2+}$에 의한 절단과 Xanthomonas celebensis 5S rRNA의 구조분석의 결과들에 기초하여 우리는5S rRNA에서의 삼차상호작용에 대한 작업가설을 제안하였다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2010년도 추계학술대회
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• pp.663-665
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• 2010

By the curing and transformation experiment, it was found thatthe genes of Pseudomonas sp.KU171(pKU19) for MCPA-degrading were located on a plasmid pKU19. Also the plasmid had degradative gens for 2,4-D, 3CB, and DCP. Molecular size of pKU19 was measured to be 31.2Kb. The restriction pattern were analyzed with Eco RI, BglII,XhoI, and the restriction map was generated.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2009년도 추계학술대회
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• pp.1091-1094
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• 2009

In order to find the characteristics of selected powerful Pseudomoanas sp. KU171(pKU19) degrading MCPA, many physiological and genetic tests were accomplished. By the curing and transformation experiment, it was found that the genes of Pseudomonas sp.KU171(pKU19) for MCPA-degrading were located on a plasmid pKU19. Also the plasmid had degradative gens for 2,A-D, 3CB, and DCP. Molecular size of pKU19 was measured to be 31.2Kb.

• 미생물학회지
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• 제32권1호
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• pp.1-6
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• 1994

광합성 세균과 비광합성 세균에 속하는 종들 사이의 유연관계를 파악하기 위해 DNA 혼성화 방법을 실시하였다. 혼성화도는 종내 균주들 사이와 Rhodobacter capsulatus와 Rhodopseudomonas blastica 사이를(72-88%) 제외하고는 전체적으로 낮게 나타났다(2-35%). 광합성 세균 Rhodopseudommonas Palustris와 비광합성 세균 Pseudomonas aeruginosa ATCC 27853, Bradyrhizobium japonicu, 사이의 D%는 광합성 세균 사이의 D%보다 약간 높게 나타났다(26-33%). Rhodopseudomonas blastica와 Rhodobacter capsulatus 사이의 D%는 72%로 유전적 유연관계가 매우 높은 것으로 나타났다.

• 한국식품영양과학회지
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• 제24권4호
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• pp.631-635
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• 1995

In order to develop effective manufacturing method and to improve quality of low-salt fermented squid(10% of table salt), we investigated the effects of temperature, salinity and pH on the growth of Staphylococcus xylosus, Micrococcus varians, Pseudomonas diminuta and Pseudomonas D2 isolated from of low-salt fermented squid and the growth characteristics of these bacteria during fermentation were elucidated. All bacteria showed good growth during the process of low-salt fermented squid(pH 6~7 ; concentration of NaCl, 7~10% ; temperature, 7~1$0^{\circ}C$) and their cell numbers increased as fermentation proceeded under the same fermentation condition.

• 대한화학회지
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• 제42권2호
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• pp.209-213
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• 1998

(η6-mesitylene) manganese (Ⅰ) tricarbonyl hexafluorophosphate[MTH-Mn (Ⅰ)]과 황산 이메틸, 피로탄산 이에틸, 과망간산 칼륨 따위 화학탐침들을 사용하여 Pseudomonas alcaligenes 5S rRNA의 고차원 구조를 분석하였다. 5S rRNA의 삼차구조에서 MTH-Mn (Ⅰ)이 강하게 절단하는 자리들은 a고리의 $G_{12}AUGG_{16}$, b-C 구역의 3'쪽 가닥, 즉$G_{51}AAGUGAAGC_{60}$, B-a구역의 $U_{65}-AGCG_{69}$, d고리의 5'쪽 가닥의 $G_{72}AUGG_{76}$ 연속부분 들이다. MTH-Mn(Ⅰ)과 그밖의 화학 탐침들을 사용하여 얻은 절단 양식들에서 우리는, MTH-Mn(Ⅰ)으로 강하게 절단되는 연속부분들이 $tRNA^{Phe}$의 L자 구조의 모서리 부분에서와 같은 주머니 구조를 이룰 것이며, 이러한 구조를 형성할 때 b-C구역과 d고리가 돌쩌귀 구실을 하는 것으로 추정한다.

• 한국토양비료학회지
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• 제29권4호
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• pp.396-402
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• 1996

식물생육촉진 유용미생물을 지리산 일대의 침엽수와 활엽수 밑의 부엽토(腐葉土)에서 선발하고, 고추에 접종하였을때 고추 묘소질과 생육 및 수량에 미치는 영향에 대한 결과를 요약하면 다음과 같다. 1. 혼합배양결과 Pseudomonas P배지는 $25^{\circ}C$에 Micrococcus sp., Enterobacter agglomerans, Pseudomonas putida, Pseudomonas fluorescens등 4균주, Sabouraud dextrose배지에서는 $35^{\circ}C$에서 Bacillus subtilis, Cellulomonas sp., Bacillus megaterium, Staphylococcus xylosus등 4균주의 생육이 양호하였다. 2. 혼합균주 처리구는 무처리구에 비해 초장, 분지수등은 증가하였고, EC 수준이 높아질수록 초장은 작아진 반면, 분지수는 혼합균주 처리 58일(日) 이후 EC 2.9ds/m에 비해 EC 8.6 dS/m에서 많아지는 경향이었다. 수량은 혼합균주 처리구가 무처리구에 비해 13% 증수되었고, EC 수준이 높아질수록 감소하는 경향이었다. 3. 시험후 토양의 EC, $P_2O_5$, K, Ca, Mg, $NO_3-N$은 EC 수준이 높아질수록 현저하게 증가하는 경향이었으며, 혼합균주 처리구는 무처리구에 비해 OM 및 $NH_4{^+}-N$은 낮은 반면, EC, $P_2O_5$, K, Ca, Mg, $NO_3-N$은 높은 경향이었다. 4. 시험후 혼합균주 처리구는 무처리구에 비해 수분율이 높은 경향이었으며, EC 수준이 높아 질수록 가밀도 수분율, 공극율 등은 증가하는 경향이었다. 5. 혼합균주 처리구는 무처리구에 비해 과실과 엽에서 T-N, $P_2O_5$, $K_2O$, CaO, MgO함량이 높았으며, EC 수준이 높아질수록 $P_2O_5$, $K_2O$, MgO 함량은 증가한 반면, CaO 함량은 낮아지는 경향이었다.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.812-821
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• 2007

The ecosystems of certain abandoned mines contain arsenic-resistant bacteria capable of performing detoxification when an ars gene is present in the bacterial genome. The ars gene has already been isolated from Pseudomonas putida and identified as a member of the membrane transport regulatory deoxyribonucleic acid family. The arsenite-oxidizing bacterial strains isolated in the present study were found to grow in the presence of 66.7 mM sodium arsenate($V;\;Na_2HAsO_4{\cdot}7H_2O$), yet experienced inhibited growth when the sodium arsenite($III;\;NaAsO_2$) concentration was higher than 26 mM. Batch experiment results showed that Pseudomonas putida strain OS-5 completely oxidized 1 mM of As(III) to As(V) within 35 h. An arsB gene encoding a membrane transport regulatory protein was observed in arsenite-oxidizing Pseudomonas putida strain OS-5, whereas arsB, arsH, and arrA were detected in strain OS-19, arsD and arsB were isolated from strain RW-18, and arsR, arsD, and arsB were found in E. coli strain OS-80. The leader gene of arsR, -arsD, was observed in a weak acid position. Thus, for bacteria exposed to weak acidity, the ars system may cause changes to the ecosystems of As-contaminated mines. Accordingly, the present results suggest that arsR, arsD, arsAB, arsA, arsB, arsC, arsH, arrA, arrB, aoxA, aoxB, aoxC, aoxD, aroA, and aroB may be useful for arsenite-oxidizing bacteria in abandoned arsenic-contaminated mines.