• Microbiology and Biotechnology Letters
• /
• v.32 no.1
• /
• pp.52-59
• /
• 2004

When both fructose and galactose were added to a production medium as carbon sources, the productivity of PAFS (Psedomonas Antifungal Substance) biosynthesized by Pseudomonas aeruginosa was observed to be reduced significantly due to the well-known phenomenon of catabolite repression. In order to overcome this phenomenon by use of fermentation bioprocess, fed-batch cultivation method was examined. In addition, a high producer mutant strain, AP-20 obtained by a rational screening method was tested for its productivity of PAFS in both batch and fed-batch fermentation processes. Notably fed-batch operation showed approximately 4 fold higher PAFS productivity than traditional batch operation process. It was appeared that galactose was utilized principally for the cell growth of Pseudomonas aeruginosa whereas large portion of fructose was used for the biosynthesis of PAFS. Furthermore it was observed that composition and feeding rate of production media should be optimized even in the fed-batch fermentation bioprocess. As an example, very slow feeding of carbon sources gave rather negative effect on the production of PAFS due to significant limitation of carbon and energy sources available for the producer microorganism.

• Korean Journal of Microbiology
• /
• v.24 no.3
• /
• pp.302-307
• /
• 1986

In order to investigate the properties of cephalospornase, a strong antibiotic resistant strain(H 112) was isolated from Pseudomonas aeruginose. The extracted enzyme had the following characteristics. Optimum temperature was 45.deg.C and unstable over $55^{\circ}C$, and optimum pH was 8.5. Cephalosporinase activity was not inhibited by metal ions such as $Mn^{++},\;Cu^{++}\;Fe^{++},\;Fe^{+++}$, and EDTA. But it was inhibited by some antibiotics such as carbenicillin, cefoxitin, cefotaxime, cefamandole, cefoperazone and SDS. The Vmax values of the enzyme were 100 at cephaloridine and 2.8 at cefoperazone, respectively. The molecular weight of cephalosporinase was estimated to be about $37.500{\pm}3,000$ by high performance liquid chromatography and nitrocefin reaction.

• Korean Journal of Microbiology
• /
• v.50 no.4
• /
• pp.384-386
• /
• 2014

Pseudomonas aeruginosa is an opportunistic pathogen that inhabits various natural and artificial environments, such as pathogenesis, water, soil and air. They can cause serious problems, such as pathogenic infection. In this study, 220 colonies were isolated from water and soil environment that assumed to be P. aeruginosa using a membrane filter method based on International Organization for Standardization (ISO/NP 16266). Identification of the isolates was determined by physiobiochemical characteristics using newly modified ISO method which includes the resistance to 1,10 phenanthroline test. Only one of 220 presumed P. aeruginosa strains isolated from effluence water using a drain swab was determined as P. aeruginosa-positive by the ISO/NP 16266 method. Subsequently, the resistance to 1,10 phenanthroline test, which was newly proposed by ISO in 2014 and applied in this study, was considered as more precise and improvable method for identification of P. aeruginosa.

• Microbiology and Biotechnology Letters
• /
• v.22 no.1
• /
• pp.92-98
• /
• 1994

The bacteria which secrete surface-active agent and decrease the surface tension of culture broth were isolated from soil samples. Among them, biosurfactant producing strain KK-7 was selected and emulsification was also detected. The KK-7 produced biosurfactant not only lipid but also glucose by using carbon source. Taxonomical characterization tests have demostrated the strain KK-7 to be Pseudomonas aeruginosa. The media composition of the P. aeruginosa KK-7 for the biosurfactant production was 1% glucose, 0.5% tryptone, 0.2% yeast extract, 0.15% potas sium phosphate mono-dibasic, 0.05% MgSO$_{4}$, initial pH 8.5, at 30$\circ $C for 2 days. In this condition, the concentration of biosurfactant was reached CMC 5 in the culture broth. Surface active material was produced maximum at stationary8 phase, but emulsification power was higher at log phase than stationary phase. It was considered that P. aeruginosa KK-7 produced biosurfactant more than one type having defferent properties and each maximum production time was different. The minimun surface tension of biosurfactant in 50 mM Tris buffer (pH8.0) was 28 dyn/cm, and CMC was 1 g/L.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.8
• /
• pp.1539-1548
• /
• 2017

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that commonly causes fatal infections in cystic fibrosis and burn patients as well as in patients who are hospitalized or have impaired immune systems. P. aeruginosa infections are difficult to treat owing to the high resistance of the pathogen to conventional antibiotics. Despite several efforts, no effective prophylactic vaccines against P. aeruginosa are currently available. In this study, we investigated the activity of the CIA06 adjuvant system, which is composed of alum and de-O-acylated lipooligosaccharide, on a P. aeruginosa outer membrane protein (OMP) antigen vaccine in mice. The results indicated that CIA06 significantly increased the antigen-specific IgG titers and opsonophagocytic activity of immune sera against P. aeruginosa. In addition, the antibodies induced by the CIA06-adjuvanted vaccine exhibited higher cross-reactivity with heterologous P. aeruginosa strains. Finally, mice immunized with the CIA06-adjuvanted vaccine were effectively protected from lethal P. aeruginosa challenge. Based on these data, we suggest that the CIA06 adjuvant system might be used to promote the immunogenicity and protective efficacy of the P. aeruginosa OMP vaccine.

• Proceedings of the Korean Institute of Industrial Safety Conference
• /
• 1998.05a
• /
• pp.171-176
• /
• 1998

공업의 고도 성장과 이로 인하여 유출되는 산업폐기물의 함유된 각종 중금속류가 무한한 잔류독성을 가지므로 이를 제거하려는 관심이 높아지고 있다. 오염원 중 특히 카드뮴, 수은, 니켈, 크롬, 납등에 의한 환경오염은 자연의 생태계를 변화시켜 우리의 생활권까지 위협을 주고 있어 더욱 문제가 되고 있다. (중략)

• Proceedings of the Korean Institute of Industrial Safety Conference
• /
• 1998.05a
• /
• pp.153-158
• /
• 1998

산업발달과 더불어 우리의 일상 생활과 밀접한 관계를 갖게된 각종 중금속은 직접적으로는 직업병을 유발함음 물론 간접적으로는 식품류, 수질, 대기 및 토양 등을 오염시켜 만성적 혹은 급성적로 인체에 피해를 가져온다. 이와 관련해서 특히 문제시되는 중금속류로는 납, 카드뮴, 수은, 니켈, 크롬, 비소 등이 있다. 이중에서도 카드뮴에 의한 중독은 대표적인 예의 하나이다. (중략)

• Korean Journal of Veterinary Service
• /
• v.36 no.3
• /
• pp.171-180
• /
• 2013

This study was carried out to investigate the antimicrobial resistance pattern and distribution of resistance gene in 44 Enterobacteriaceae and 21 Pseudomonas (P) aeruginosa isolated from hospitalized dogs and cats in animal hospital from 2010 to 2011 in Daegu. Among Enterobacteriaceae, Escherichia (E) coli was highly resistant to ampicillin (56.7%), followed by tetracycline (53.3%), cephalothin, streptomycine, sulfamethoxazole/trimethoprim, gentamicin and norfloxacin (40.0~43.3%). The remaining isolates of Enterobacteriaceae had high resistance to ampicillin (64.3%) and streptomycin (42.9%). Whereas, P. aeruginosa was low resistant to all antimicrobials tested (less than 15%). int I 1 gene was detected in 20 (57.1%) of 35 antimicrobial resistant Enterobacteriaceae and 2 (9.5%) of 21 P. aeruginosa., but int I 2 gene was not detected in all isolates. The eight resistance genes were found either alone or combination with other gene (s): $bla_{TEM}$, aadA, strA-strB, clmA, tetA, tetB, sul I and sul II. About 78% of integron-positive isolates were resistance to more than four antimicrobial agents. The findings suggest that class I integrons are widely distributed in E. coli among Enterobacteriaceae from dogs and cats and multi-drug resistance related to the presence of class I integrons. The prudent use of antimicrobials and continuous monitoring for companion animals are required.

• Korean Journal of Clinical Laboratory Science
• /
• v.39 no.2
• /
• pp.68-75
• /
• 2007

After reports on regression of cancer in humans and animals infected with microbial pathogens date back more than 100 years, much effort has been spent over the years in developing a wild type or attenuated bacterial and purified bacterial proteins for the treatment of cancer. Pseudomonas aeruginosa exotoxin A (ETA) is known to inhibit cell growth and trigger significant cell death in various cancer cells. Although ETA induces apoptosis of cancer cells, its exact mechanism of action is not known yet. Four different assays were performed in this study: morphological assessment of apoptotic cells, cell cytotoxity, annexin-V binding assay, and cell cycle analysis. The proliferation and survival of the K-562 cells treated with ETA were decreased in a dose dependent manner. In addition, the apoptotic body of K-562 cells was induced by ETA treatment in a dose dependent manner. The ETA-induced apoptosis was confirmed by annexin-V binding assay. Flow cytometric analysis was examined to ascertain whether ETA could arrest the cell cycle at the sub-G1 phase. Our results suggest that P. aeruginosa ETA inhibits cell growth and induces apoptosis in human leukemia K-562 cells.

• Journal of Life Science
• /
• v.11 no.5
• /
• pp.483-488
• /
• 2001

The antimicrobial substance produced by Pseudomonas aeruginosa KLP-2 strain was purified and identified. The substance was identified as a pyocyanine by the fast atom bombardment mass(FAB-MS). In physic-chemical properties, the pyocyanine was dark blue needles, and was soluble in various organic solvents such as chlorogorm, methanol, ethanol and ethyl acetae. The pyocyanine possessed a ultraviolet absorbance spectrum in methanol, 0.1 M HCl, and chlorogorm. The maximum absorption peak of the pyocyanine showed at 318 mm in methanol. The molecular formula of the pyocyanine was determined to the $C_{13}$ H$_{10}$ N$_{2}$O and protonate molecular ion species (M+H)$^{+}$ was observed at m/z 211 by FAB-MS. The pyocyanine showed antimicrobial against Bacillus cereus, Bacillus subtilis, Micrococcus luteus, Rodococcus equi, Staphylococcus aureus, Streptococcus faecalis, E. col, Legionella pneumophila, Shigella flexneri Shigella boydii, shgella sonnei, NAG Vibrio cholerae, Vibrio parahaemolyticus, Vibro vulnificus, Yersinia enterocolitica, and Saccharomyces cerevisiae. However, Salmonella spp. Shigela dysenteriae, 3 strains of Pseudomonas aeruginosa, Klebsiela pneumoniae, and Aspergillus niger were resistant to the pyocyanine. The pyocyanine showed the highest antimicrobial activity aganist Legionella pneumophila based on the size of inhibition zone by the disk contained 0.5 $\mu\textrm{g}$ of the pyocyanine.e.