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Distribution of Indicator Organisms and Influence of Storage Temperature and Period in Commercial Plant Food (시판 식물성 식품의 오염지표세균 분포 및 저장온도, 기간별 오염지표세균의 변화)

  • 이용욱;박석기
    • Journal of Food Hygiene and Safety
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    • v.14 no.1
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    • pp.1-8
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    • 1999
  • There were few data for the distribution of the indicator organisms in the commercial plant foods, and for the normal flora and for the foodborne agents within the country. First of all it must be investigated the distribution of the indicator organisms. And also it is very important to prepare the sanitation criteria for the plant foods through the microbiological examination and the investigation of tendency to change of the indicator organisms according to the storage temperature and period. The average number of total viable counts for grains was 2.9$\times$105/g, psychrophilic bacteria 2.9$\times$105/g, heterotrophic bacteria 3.1$\times$105/g, heat-resistant bacteria 2.1$\times$103/g, Pseudomonas aeruginosa 23/g. That for beans was 6.3$\times$102/g, psychrophile 34/g, heterotroph 1.7$\times$102/g. That for sesames was 1.4$\times$105/g, coliform 350/g, psychrophile 7.4$\times$104/g, heterotroph 5.8$\times$104/g, Pseud. aeruginosa 2.3$\times$103/g. heat-resistant bacteria 150/g. That for potatoes was 2.0$\times$107/g, coliform 5.0$\times$104/g, psychrophile 1.8$\times$107, heterotroph 1.4$\times$107/g, heat-resistant bacteria 3.3$\times$104/, Staphylococcus 2.7$\times$105/g, fecal streptococcus 4.5$\times$103/g, Pseud. aeruginosa 7.0$\times$103/g. That for mushrooms was 1.2$\times$108/g, psychrophile 9.4$\times$107/g, heterotroph 1.0$\times$109/g, heat-resistant bacteria 1.6$\times$105/g, Pseud. aeruginosa 1.3$\times$103/g. That for vegetables was 5.9$\times$1011/g, coliform 1.8$\times$106g/, Staphylococcus 1.1$\times$1012/g, heterotroph 8.4$\times$1011/g, heat-resistant bacteria 7.6$\times$106/g, Staphylococcus 1.1$\times$107/g, fecal streptococcus 1.1$\times$104/g, Pseud. aerugniosa 5.2$\times$104/g. That for nuts 3.9$\times$104/g, coliform 3.9$\times$103/g, psychrophile 4.0$\times$104/g, heterotroph 3.2$\times$104/g, heat-resistant bacteria 400/g. In commercial grains and beans, SPC, psychrophile, heterotroph and heat-resistant bacteria stored at 1$0^{\circ}C$, 2$0^{\circ}C$, 3$0^{\circ}C$ were constant. Staphylococcus, coliform, Pseud. aeruginosa were decreased a little n grains, but were not detected in beans. In mushrooms, all indicator organisms were increased as time goes on and were increased rapidly at 2$0^{\circ}C$. In sesames, coliform was not detected at all temperature. psychrophile was increased for 7 days, the others were constant. In potatoes, SPC, psychrophile, heat-resistant bacteria, heterotroph had a tendency to increase and the others were constant. In vegetables, indicator organisms were had a tendency to increase, psychrophile, heterotroph were rapidly increased after 7 days. In nuts, SPC, coliform, psychrophile heterotroph, heat-resistant bacteria, Pseud. aeruginosa were constant, staphylococcus and fecal streptococcus were not detected.

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Identification of 4-Chlorobenzoate Dechlorinating Bacteria by Simple Plate Assay (평판배지법에 의한 4-chlorobenzoate 탈염소화 세균의 검색)

  • Chae, Jong-Chan;Kim, Chi-Kyung;Min, Kyung-Hee;Park, Yong-Keun
    • Microbiology and Biotechnology Letters
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    • v.23 no.1
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    • pp.104-109
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    • 1995
  • The gene responsible for dechlorination of 4-chlorobenzoate (4CBA) was cloned in E. coli XL1-Blue from Pseudomonas sp. DJ-12. The cloned cell of E. coli Cjl had the hybrid pBluescript SK(+) plasmid, into which about 9.5 kb genomic DNA fragment of PseudOmonas sp. DJ-12 was inserted. The subclone of pCJlOl was constructed by inserting the 3.4 kb EcoRI-HindIII fragment of pCJl into the vector. Those cloned cells could be simply selected by halo formation around the colonies which was the precipitate of AgCl produced by reaction of AgNO$_{3}$ and chloride ion liberated by bacterial dechlorination of 4CBA- Such a plate assay method was standardized by the procedure that the colonies grown for 2 days on the Cl$^{-}$-free plate medium containing 1 mM 4CBA were flooded with 0.1 M AgNO$_{3}$ solution.

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On the Utilization of the Allometric Method for an Estimation of the Productivity of the Terrestrial Plants (육상식물의 생산력추정을 위한 상대상장법의 이용에 대하여)

  • 김준호
    • Journal of Plant Biology
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    • v.13 no.1
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    • pp.47-55
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    • 1970
  • In order to approach a reasonable method for the indirect estimation of the productivity in terrestrial plants, allometric method was studied with herbs, such as Boenmeria frutescens and Hibiscus mutabilis, and scrubs, such as Robinia pseud oacacia and Morus alba f. Hchiroe. Allometric relations between D230H, square of the stem(or shoot) diameter(D30) on 30cm height above ground multiplied by stem length(H), and the amount of the stem(Ws), orof the leaves per stem, were fitted to each material plant with linear regression. The allometric coefficient in relation of D230H to Ws was approximated between scrubs having different life form, and the allometric function in relation of DF2 to WL was common between herbs in spite of having different growth habitat. As the allometries between D230H and Ws or WL were changeable with the seasonal change it funciton should be calculated repeatedly whenever investigating the standing crop of such community. It is able to utilize an indirect value of D30 obtained from the allometry between D30 and H because direct measurement of D30 in the field is accompanied by troublesome and waste of much time.

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Unrecorded causal organisms of Korean Powdery (한국산 미기록 백섭병균류에 관한 연구)

  • 이호준;이배함
    • Korean Journal of Microbiology
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    • v.5 no.1
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    • pp.24-33
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    • 1967
  • Fifty-two specimens of the infected plants were collected from areas through the country during the year of 1965. We report here 14 unrecorded specoes which were identified in this work. The results are as follows: 1. Erysiphe cichoracearum DC. (Host: Artemisia princeps PAMPAN. var. orien-talls HARA.) 2. Erysiphe cichoracearum DC. (Host: Plantago sp.) 3. Erysiphe cichoracearum DC. (Host: Sonchus oleraceus L.) 4. Erysiphe pisi DE CANDOLLE. (Host: Lespedeza sp.) 5. Erysiphe polygoni DE CANDOLLE. (Host: Clematis patens MORR et DECAIS.) 6. Microsphaera alphitoides GRIFFITHS et MAUBLANCE (Uncinula bifurcata GERARD.) (Host: Quercus serrate GERARD) 7. Microsphaera baumleri MAGNUS. (Host: Robinia pseud-acacia L.) 8. Microsphaera coryli HOMMA. (Host: Corylus heterophylla FISCH. var. japonica KOIDZUMI) 9. Podosphaera tridactyla (WALLROTH) DE BARY. (Host: Prunus leveilleana KOEHN. var. typica NAKAI) 10. Sphaerotheca fuliginea (SCHLECHTENDAHL) POLLACI. (Host: Impatiens balsamina L.) 11. Sphaerotheca fuliginea (SCHLECHTENDAHL) POLLACI. (Host: Zinnia elegans JACQ) 12. Uncinula aceris SACCARDO (Host: Acer negundo L.) 13. Uncinula fraxini MIYABE(U. salmon SYDOW) (Host: Fraxinus rhynchophyllus HANCE.) 14. Uncinula salicis (DE CANDOLLE) WINTER. (Host: Salix gracilistyla MIQ.)

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Application of Pseud-superplastic PM Process to Ti-Al Intermetallic Compound for MEMS Parts

  • Miyano, Naoki;Kumagai, Yusuke;Yoshimoto, Masayoshi;Nishimura, Yuta;Tanaka, Shigeo;Ameyama, Kei
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09b
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    • pp.1292-1293
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    • 2006
  • A non-equilibrium powder metallurgy processing such as an MA/SPS (Mechanical Alloying / Spark Plasma Sintering) process is examined in a Ti-48moll%Al. TiAl intermetallic compound is a potential light-weight/high-temperature structural material. One of the major problems, however, limiting the practical use of the material is its poor workability. From this point, the powder metallurgy (PM) processing route has been attractive alternative of the conventional processing for such material The MA/SPS process is able to apply to a LIGA process. Optimization of the pseudo-superplasticity enables to fabricate micro-parts made of fine grained ceramics composites of TiAl by the LIGA process.

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Numerical Simulation of Pseudo-Shock Waves with Different Confinement Parameters (서로 다른 Confinement parameter를 가지는 의사충격파의 전산유동해석)

  • Kang, Kyungrae;Choi, Jong Ho;Song, Seung Jin;Do, Hyungrok
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2017.05a
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    • pp.336-340
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    • 2017
  • When supersonic flow is through an internal duct, there forms a flow structure called pseudo-shock. Pseudo-shock is a result of shockwave-boundary layer interaction(SBLI) and to simulate pseudo-shock correctly, one needs to correctly anticipate not only the strength of the shock but also the boundary layer behavior as well. In this study, pseud-shockwave structure at a rectangular duct will be numerically simulated using dedicated inlet boundary conditions to obtain accurate solution in terms of its structure and pressure rise pattern.

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Purification and Properties of .$\beta$-1, 3-Glucanase from Pseudomonas stutzeri KF13 (Pseudomonas-stutzeri KF13의 ..$\beta$-1, 3-Glucanase 정제 및 성질)

  • 방광웅;송형익;김재근;유대식;정기택
    • Korean Journal of Microbiology
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    • v.25 no.1
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    • pp.1-8
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    • 1987
  • An extracellular $\beta$-1, 3-glucanase from Pseudomonas stutzeri KF 13 was purified about 390 with 26% recovery. The purified enzyme revealed a single band by polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis. The enzyme was stable in a pH 6.0 to 9.0, and relatively thermostable. The optimal pH and temperature on the enzyme activity were found to be 5.8 and 45.deg.C, respectively. The activation energy was calculated to be 16,130 cal per mole. The Km value for laminarin was found to be 3ng per ml and the molecular weight was determined to be 28,000 by gel filtration and 26,000 daltons by SDS-acrylamide gel electrophoresis. The enzyme was inhibited by 1.0mM of $Hg^{2+}$, and strongly inhibited by 1.0mM of p-chloromercuribenzoic acid.

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Chemical Composition and Antimicrobial Activities of Houttuynia cordata Thunb. (어성초의 화학성분 및 항미생물 활성)

  • Kim, Keun-Young;Chung, Dong-Ok;Chung, Hee-Jong
    • Korean Journal of Food Science and Technology
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    • v.29 no.3
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    • pp.400-406
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    • 1997
  • Chemical composition and antimicrobial activity of Houttuynia cordata Thunb. were investigated to develop a natural food preservative from it. Aspartic acid, glutamic acid, glycine and arginine were major amino acids of Houttuynia cordata Thunb., but were present in a trace amount. Free sugars were composed of glucose, fructose, sucrose and maltose and major fatty acids were linolenic acid, linoleic acid, oleic acid, and palmitic acid. Mineral elements were potassium, calcium, magnesium, iron, zinc, and copper. Antimicrobial activities were shown in acidic, neutral and phenolic fraction of Houttuynia cordata Thunb., but not in basic fraction. Among the four fractions, neutral fraction showed the strongest antimicrobial activities against microorganisms tested, such as Pseudomnas aeruginosa, Micrococcus luteus, Bacillus subtilis, Aspergillus parasiticus and Aspergillus flavus. Minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) of the neutral fraction varied according to microoganisms tested. The lowest values of MIC (0.0075 g eq./mL) and MLC (0.10 g eq./mL) were obtained from Pseudomonas aeruginosa.

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Structure-Activity Relationships of Peptide Antibiotics with Improved Bacterial Cell Selectivity of Pseudin

  • Lee, Yeongjoon;Jeon, Dasom;Kim, Jin-Kyoung;Kim, Yangmee
    • Journal of the Korean Magnetic Resonance Society
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    • v.21 no.3
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    • pp.78-84
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    • 2017
  • Pseudin is a naturally occurring 24 amino-acid-residue antimicrobial peptide derived from the skin of paradoxical frog Pseud's paradoxa. It shows potency against the bacteria and antibiotic-resistant bacteria strain, but has high cytotoxicity against mammalian cell. In our previous study, substitution of $Pro^{11}$ for Gly (Ps-P) increased bacterial cell selectivity but decreased the antibacterial activity of pseudin. In this study, we designed pseudin analogue, Ps-4K-P with increased cationicity up to +7 in Ps-P by substituting Glu14, Gln10, Gln24, and Leu18 with Lys. Ps-4K-P showed improved potent antibacterial activity with high bacterial cell selectivity. We determined the tertiary structure of Ps-4K-P in the presence of DPC micelles by NMR spectroscopy and it has a hinge structure at $Pro^{11}$ followed by three turn helices from $Pro^{11}$ to $Val^{23}$ at the C-terminus. Amphipathicity with increased cationicity as well as helix-hinge-helix structural motif provided by introduction of a Pro at position $Gly^{11}$ are the crucial factors which confer antibacterial activity with bacterial cell selectivity to Ps-4K-P.

Synthesis of Copolymeric PHA by Hydrogenophaga pseudoflava and Ralstonia eutropha H16 from Vari-ous Lactones and Their Microstructural Studies (락톤류로부터 Hydrogenophaga pseudoflava와 Ralstonia eutropha H16 두 세균에 의한 공중합 PHA의 합성 및 미세구조적 특성 연구)

  • Jang, Young-Ok;Nam, Won;Choi, Mun-Hwan;Song, Jae-Jun;Yoon, Sung-Chul
    • Microbiology and Biotechnology Letters
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    • v.28 no.2
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    • pp.71-79
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    • 2000
  • Two typess of copolyesters, poly(3-hydroxybutyric acid-co-4-hydroxy-butyric acid)[P(3HB-co-4HB] and poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid)[P(3HB-co-3HV)], with various monomer ratios and different degree of microstructural heterogeneity were synthesized from Ralstonia eutropha H16 and Hydrogenophaga pseudoflava by using ${\gamma}$-butyrolactone and ${\gamma}$-valerolactone, respectively. The two bacteria showed a large difference in the utilization of ${\gamma}$-butyrolactone for cell growth and PHA synthesis. H. pseudoflava synthesized P(3HB-co-4HB) copolyesters with a wide range of 4HB content from 13 to 96 mol% depending on culture conditions, whiel R. eutropha H16 was able to synthesize the copolyesters containing less than 20 mol% of 4HB. An increase in the 4HB content in the P(3HB-co-4HB) copolyesters synthesized by H. pseud-oflava induced an lowering of their melting temperatures as well as their enthalpies of fusion. The increase in the 4HB content, however, increased the rate of degradation by an extracellular P(3HB) depolymerase. NMR spectros-copy and differential scanning calorimetry showed that the P(3HB-co-4HB) copolyesters from H. pseudoflava were generally microstructurally heterogeneous. The P(3HB-co-4HB) copolyesters) synthesized by R. eutropha H16 were rather random copolymers showing less microstructural heterogeneity than those synthesized by H. pseudoflava. The NMR D value analysis suggested that the monomer distribution of the P(3HB-co-3HV) copolymers from the two bacteria were relatively random.

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