• Mycobiology
• /
• v.38 no.4
• /
• pp.323-327
• /
• 2010

This study contains descriptions and illustrations of three new and noteworthy taxa of the genus Psathyrella from Korea. Among them, Psathyrella pervelatoid, P. koreana, and P. utriformcystis are new to science. All collections cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

• YAKHAK HOEJI
• /
• v.45 no.6
• /
• pp.617-622
• /
• 2001

A protein-polysaccharide fraction of a Korean wild mushroom Psathyrella velutina, PVP, was prepared and its antitumor immunomodulatory activity was investigated. When PVP was administered once daily for seven days from day 1 to day 7 into male ICR mice implanted with 1 $\times$ 10$^{5}$ cells of sarcom 180 tumor cells into the peritoneum on day 4, it inhibited the growth of sarcoma 180 cells by 92.8%. In XTT assay, PVP also exhorted in vitro anti-proliferation activity on U-937, a human monoblastoid cell line, as well as sarcoma 180 cells. PVP showed marked stimulatory activity on the immune system in that it induced the accumulation of PEC (the stimulation index, Sl=4.90 at 100 mg/kg), stimulated the BALB/c mouse splenic lymphocytes to form lymphoblasts (Sl=5.75 at 100$\mu\textrm{g}$/ml), and upergulated the expression of CD25 molecules. All these results strongly support that PVP exhorts its antitumor activity through stimulation of the immune system as well as anti-proliferative activity on the tumor cells.

• YAKHAK HOEJI
• /
• v.48 no.5
• /
• pp.261-265
• /
• 2004

In the previous report, we described the marked antitumor and immunomodulatory activities of PVp, a protein-polysaccharide fraction of a Korean wild mushroom Psathyrella velutina. In this study, a protein-polysaccharide fraction, PVMP, was prepared from the shake-cultured mycelia of the same mushroom and its immunoactivities as well as chemical compositions were investigated. At 200 $\mu\textrm{g}$/ml, PVMP weakly stimulated the BALB/c mouse splenic lymphocytes to form lymphoblasts and upregulated the expression of CD25 molecules, but failed to stimulate peritoneal macrophages. In chemical analysis these two protein-polysaccharide fractions were found to be quite different in that the carbohydrate contents of PVMP and PVP, respectively, was 85.3% and 41.2%. These results reveals that PVMP, unlike PVP, is a moderate immunostimulator on the immune system.

• The Korean Journal of Mycology
• /
• v.27 no.4 s.91
• /
• pp.274-279
• /
• 1999

The internal transcribed spacer regions(ITS) of the ribosomal DNA gene repeat from Coprinus and Psathyrella spp. were amplified using polymerase chain reaction (PCR) and sequenced. Sequences from 11 species including Coprinus comatus, C. atramentarius, C. micaceus, C. cinereus, C. rhizophorus, C. radians, C. echinosporus, C. disseminatus, Psathyrella candolleana, P. spadiceogrisea and Stropharia rugosoannulata were compared. The spacer region I and II were $258{\sim}301\;bp\;and\;253{\sim}275\;bp$ in length respectively and partially contained 17S, 5.8S and 25S. The reciprocal homologies of ITS sequences among these strains were in the range of $43.9{\sim}96.0%$. According to the analysis of ITS sequences, Coprinus and Psathyrella spp. were classified into three clusters. Cluster I consisted of Coprinus lagopus, C. cinereus, C. echinosporus, C. rhizophorus, and C. atramentarius. Cluster II comprised C. micaceus, C. radians, C. disseminatus, Psathyrella candolleana, and P. spadiceogrisea. On the other hand C. comatus is in Cluster III with Stropharia rugosoannulata even though this species is belonging to the section Coprinus in morphological aspect. These results suggest that taxonomic position of Psathyrella would better be inculded in genus Coprinus. Coprinus comatus, the type species of Coprinus, gives a doubt on monophyletic evolution and is assumed to be paraphyletic or polyphyletic.

• Mycobiology
• /
• v.38 no.4
• /
• pp.249-255
• /
• 2010

We identified Lacrymaria velutina of the Coprinaceae in Korea. The unusually large and sturdy fruiting body, fibrillose to fibrillose-scaly cap and stalk without a volva with an obscure superior hairy ring zone or hairy annulus, and blackish brown, warted spores distinguished this species from closely related Psathyrella species. An illustrated account of the microscopic traits is presented. Fruiting bodies with obtusely hemispherical caps, 2.5~6 cm, becoming convex with age; surface dry, densely fibrillose-scaly with split margin; stipe, 4.5~6 cm, equal, hollow, fibrillose, dry, whitish above the superior ring zone, light brown below; crowded gills, adnexed, dark black at maturity. Pileipellis typically cellular with the gill edge appearing white and beaded. Blackish brown basidiospores that discolor in concentrated sulfuric acid. Spores elliptical, warted, $9\sim11{\times}6\sim8{\mu}m$, with prominent snout-like germpores. Cheilocystidia abundant, $57\sim68{\times}19\sim25{\mu}m$, and narrowly elongated clavate, often clustered in threes or fours. Pleurocystidia rarely present, $45\sim47.5{\times}12\sim13{\mu}m$, and clavate to utriform. This trait distinguishes our sample as L. velutina from other Psathyrella spp. of the Coprinaceae, which have smooth spores. This taxon was clarified by the observation that Psathyrella spores fade in concentrated sulfuric acid. A molecular phylogenetic study revealed that our specimen was Lacrymria velutipes, which is closely related to Lacrymaria lacrymabunda. Moreover, those two species are clearly distinguishable from other Psathyrella species, which agreed with the morphologically distinctive traits described above. We believe that this is the first report of this taxon, which has not been described in Korea.

• The Korean Journal of Mycology
• /
• v.22 no.3
• /
• pp.209-215
• /
• 1994

In order to investigate the fungal Flora in Mt. Chilgap, which located in the middle west of Korea. Authors had collected wild mushrooms, during the mushroom-growing season, June, July and October, 1991. Six species new to the Korean flora are identified as follows: Callistosporium luteoolivaceum (Berk. & Curt.) Sing; Volvariella subtaylori Hongo; Psathyrella subatrata (Batsch) Gill.; Volvariella surrecta (Knapp) Sing.; Boletellus elatus Nagasawa; Xerocomus parvulus Hongo, and Callistosporium Sing. is also new genus to the Korea. All the collections cited are deposited in ASIK herbarium.

• Proceedings of the PSK Conference
• /
• 2001.10a
• /
• pp.197.1-197.1
• /
• 2001

• The Korean Journal of Mycology
• /
• v.9 no.2
• /
• pp.73-76
• /
• 1981

One hundred specimens of higher fungi were collected at Mt. Mudeung area from April to October, 1980. These higher fungi were identified and classified into 2 classes, 2 subclasses, 7 orders, 14 families, 21 genera and 33 species. Among them, Baeospora proved to be new genus in Korea. And Baeospora myosura (Fr.) Sing., Marasmius purpureostriatus Hongo, Psathyrella gracilis (Fr.) Quel., lnocybe sphaeospora Kobayasi and Trametes coccinea Fr. were found to be new in Korea.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2003.10b
• /
• pp.60-60
• /
• 2003

큰눈물버섯(Psathyrella velutina)의 균사생장 실험을 감자배지, 고구마배지, 대두박 배지를 이용하여 펠릿형성 상태를 관찰한 결과는 다음과 같다. 감자배지에서 펠릿의 수가 가장 뛰어났으며 밀도 역시 1.3g/$\ell$로 가장 높게 나타났다. 고구마 배지에서의 펠릿의 수가 가장 저조했으며 밀도 또한 0.3g/$\ell$로 가장 낮게 나타났다. 대두박 배지에서 펠릿의 수는 비교적 양호한 상태를 보였고, 그 밀도는 0.9g/$\ell$ 이었다. 공중균사의 굵기는 세 액체배지에서 5mm로 비슷한 결과를 보였지만, 고구마 배지의 공중균사는 색깔의 변색을 보여 이산화탄소가 과다 발생했음을 알 수 있었다. 큰눈물버섯의 균사생장은 감자로 만든 액체배지에서 가장 좋은 발육상태를 보였다.

• The Korean Journal of Mycology
• /
• v.7 no.2
• /
• pp.75-82
• /
• 1979

Several higher fungi were collected at Andong during the summer and fall of 1976. About 100 higher fungi were collected at Mt: Sobaek and Youngju during the same period in 1978. These higher fungi were identified. As the results, Macrocystidia proved to be new genus in Korea. And the following are also newly-found species in Korea: Hygrocybe turunda (Fr.) Karst., Hygrophorus lucorum Kalchbr., Macrocystidia cucumis (Fr.) Heim var. latifolia (Lange) Imazeki et Hongo, Agaricus subrufescens Peck, Psathyrella hydrophila (Bull. ex Fr.) A.H. Smith, Stropharia rugosannulata Farlow f. lutea Hongo, Inocybe bresadolae Mass., Inocybe multicoronata A.H. Smith, Russula farinihes Romell and Lactarius sakamotoi Imai.