• Journal of Ginseng Research
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• v.36 no.2
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• pp.211-217
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• 2012

Endophytic fungi were isolated from various tissues (root, stem, petiole, leaf, and flower stalk) of 3- and 4-year-old ginseng plants (Panax ginseng Meyer) cultivated in Korea. The isolated endophytic fungi were identified based on the sequence analysis of the internal transcribed spacer (ITS), 1-5.8-ITS 2. A morphological characterization was also conducted using microscopic observations. According to the identification, 127 fungal isolates were assigned to 27 taxa. The genera of Phoma, Alternaria and Colletotrichum were the most frequent isolates, followed by Fusarium, Entrophospora and Xylaria. Although 19 of the 27 taxa were identified at the species level, the remainder were classified at the genus level (6 isolates), phylum level (Ascomycota, 1 isolate), and unknown fungal species (1 isolate). Endophytic fungi of 13 and 19 species were isolated from 3- and 4-year-old ginseng plants, respectively, and Phoma radicina and Fusarium solani were the most frequently isolated species colonizing the tissues of the 3- and 4-year-old ginseng plants, respectively. The colonization frequency (CF%) was dependant on the age and tissue examined: the CFs of the roots and stems in the 3-year-old ginseng were higher than the CF of tissues in the 4-year-old plants. In contrast, higher CFs were observed in the leaves and petioles of 4-year-old plants, and endophytic fungi in the flower stalks were only detected in the 4-year-old plants. In conclusion, we detected diverse endophytic fungi in ginseng plants, which were distributed differently depending on the age and tissue examined.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.4
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• pp.125-134
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• 2021

This study aimed to explore the factors affecting the foreign direct investment (FDI) intentions of investors into Quang Ninh province, located in the north-eastern of Viet Nam. Researchers used two main methods, namely, Exploratory Factors Analysis (EFA) and the Structural Equation Model (SEM) based on partial least squares structural equation modeling (PLS SEM) to explore and measure the impact of factors affecting the investors' FDI intentions into Quang Ninh province. The empirical analysis used data from the survey of 206 domestic and foreign investors into Quang Ninh province, including representatives of the Board of Directors, members, and management representatives at the department level, with reliable tools (SPSS 26 and SmartPLS 3.0 software). The research results identified the following factors affecting investment into Quang Ninh: FDI attraction policies have the strongest impact on the investors' FDI intentions; it is followed by infrastructure, public services and human capital with strong effects on intentions of investors' FDI; and finally the standards of living that affects the investors' FDI intentions. There is also a positive relationship between all the factors and the investors' FDI intentions. Several recommendations are further suggested to enhance attraction of foreign direct investment into Quang Ninh province.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.2
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• pp.144-150
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• 2014

Yams (Dioscorea spp), which are edible or medicinal tuber crops, are a important crop in South Korea. Yams require a high level of soil fertility. The various cultural practices such as fertilizing and plowing were tested for marketable tuber production in Chinese yam (Dioscorea opposita). Tuber yield was also affected by organic matters in soil. Application higher level of organic matters result in increased each tuber weight and tuber yield per unit area. The nutrient absorption quantity of the plant such as nitrogen, phosphoric acid, calcium and potassium was increased from 100~120 days after planting, which time to begin tuber enlargement. The tuber yield was increased when the fertilization increased in quantity. Total yield and marketable ratio were the highest in 31~32 kg/10a of nitrogen fertilizer. From above result, income become larger with increase of marketable yield and quality improvement at 63% (27 kg/10a) level of conventional N fertilization (43 kg/10a). The tuber yield was not significantly different between with in various application level of potash fertilizer. Tuber size and weight decreased accordingly to decreased fertilizing level, so the rates of small tubers increased greatly at cultivation without chemical fertilizer. In considering the accumulation rates of allantoin in Chinese yam tubers, the apt harvest season was after October. The allantoin quantity of it was not influenced with nitrogen fertilizing. Moreover it was advantageous with decrease of chemical fertilizer and appropriate fertilizing in soil environment protection. Commercial tuber's number and yield were increased in trenching before planting with trencher compared with rotavating with tractor.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.1
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• pp.215-220
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• 2014

Objective: To determine the expression of E-cadherin, ${\beta}$-catenin, and transcription factor 4 (TCF4) proteins in gastric diseases with relation to Helicobacter pylori infection. Methods: A total of 309 patients including 60 with superficial gastritis (SG), 57 with atrophic gastritis (AG) and 192 with gastric cancer (GC), were enrolled. The expression of E-cadherin, ${\beta}$-catenin, TCF4 proteins in the gastric mucosa was detected by immunohistochemistry and H. pylori infection by immunohistochemistry and PCR. Results: The expression rates of E-cadherin were significantly higher in SG and AG than in GC (P<0.01), while those of ${\beta}$-catenin in the nucleus were significantly lower in SG and AG than in GC (P<0.05). In GC cases, the expression rates of E-cadherin, ${\beta}$-catenin and TCF4 were significantly higher in the intestinal type than in the diffuse type (P<0.05). In GC patients, the expression rate of E-cadherin was significantly higher in the presence of H. pylori than in the absence of infection (P=0.011). Moreover, the expression level of TCF4 and ${\beta}$-catenin protein was significantly higher in the nucleus and cytoplasm in H. pylori positive than in H. pylori negative GC patients, especially in those with the intestinal type (all P < 0.05). Conclusion: The expression of E-cadherin and ${\beta}$-catenin progressively decreases during the process of GC tumorigenesis, while overexpression of TCF4 occurs. H. pylori infection is associated with a significant increase in the expression of E-cadherin and ${\beta}$-catenin in the cytoplasm and nucleus in GC patients, especially those with the intestinal type.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.8
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• pp.3791-3794
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• 2012

CD44v, especially splice variants containing exon v6, has been shown to be related closely to development of different tumors. High levels of CD44v6/v7 have been reported to be associated with invasiveness and metastasis of many malignancies. The objective of this study was to detect expression of CD44v6-containing variants in patients with acute promyelocytic leukemia (APL) and evaluate the potential of CD44v6/v7 for risk stratification. Reverse transcription polymerase chain reaction (RT-PCR) followed by PCR product purification, ligation into T vectors and positive clone sequencing were used to detect CD44 v6-containing variant isoforms in 23 APL patients. Real-time quantitative PCR of the CD44v6/v7 gene was performed in patients with APL and in NB4 cells that were treated with all-trans retinoic acid (ATRA) or arsenic trioxide ($As_2O_3$). Sequencing results identified four isoforms (CD44v6/v7, CD44v6/v8/v10, CD44v6/v8/v9/v10, and CD44v6/v7/v8/v9/v10) in bone marrow mononuclear cells of 23 patients with APL. The level of CD44v6/v7 in high-risk cases was significantly higher than those with low-risk. Higher levels of CD44v6/v7 were found in three patients with central nervous system relapse than in other patients inthe same risk group. Furthermore, in contrast to ATRA, only $As_2O_3$ could significantly down-regulate CD44v6/v7 expression in NB4 cells. Our data suggest that CD44v6/v7 expression may be a prognostic indicator for APL.

• Korean Journal of Weed Science
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• v.32 no.2
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• pp.127-132
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• 2012

Field survey on the weed occurrence and the use of herbicides was carried out targeting 214 rice cultivating farmers at Gyeongsangbuk-do. 96.2% of farmers did machine planting in late May and this is 68.0% of total cultivating area. Spring plowing was first with 54.6%, followed by spring-fall and fall plowing with 24.8% and 15.0%, respectively. 75% of the farmers treated herbicide twice. 57.5% of them treated herbicide before transplanting and treated at post-emergence again. In case of only application, 84% of the farmers treated herbicide before transplanting. Twenty-three species were occurred at Gyeongsangbuk-do and Echinochloa spp. was the most dominant with 20.8%, Sagittaria trifolia was second with 10.4%, followed by Monochoria vaginalis and Sagittaria pygmaea with 9.9% and 9.7%, respectively. Sagittaria pygmaea was the most difficult to control followed by Echinochloa spp., M. vaginalis, and S. trifolia.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.9
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• pp.1313-1319
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• 2013

This study investigated the effects of low levels of water-soluble pentosans (WSP), alkaline-extractable pentosans (AEP), and xylanase on the growth and organ development of broiler chicks. Three hundred and fifty 1-d-old female broiler chicks were randomly allocated into seven experimental groups of five pen replicates, with ten chicks per replicate. The control group consumed a corn-soybean meal-based diet. Six dietary treatment groups consumed the basal diet supplemented with one of the following: WSP at 50 mg/kg (WSP50) or 100 mg/kg (WSP100); AEP at 50 mg/kg (AEP50) or 100 mg/kg (AEP100); or xylanase at 3 mg/kg (Xase3) or 6 mg/kg (Xase6). Data including the body weight, digestive organ weights, gut length, rectal digesta viscosity, and gut microflora and pH were collected on d 5, 10, and 15. When compared to the control group, WSP50 promoted body weight gain and organ growth throughout the study, calculated as 3-d averages (p<0.05). WSP100 increased weight gain and enhanced organ development (proventriculus, gizzard, and gut) on d 10 (p<0.05), but the 3-d averages were not different from the control group except for the weight of gizzard. Both Xase3 and Xase6 increased the 3-d average weight gain and the growth of the gizzard (p<0.05). WSP50 increased the digesta viscosity compared to Xase3 on d 10 and 15 (p<0.05). WSP50, Xase3, and Xase6 increased the concentration of Lactobacillus in the rectum when compared to the control group (p<0.05), but only Xase3 lowered the digesta pH in the ileum and cecum on d 10 and 15. AEP had minimal influence on the growth and organ development of broilers. The results showed that low levels of WSP, AEP, and xylanase had different effects and underlying mechanisms on the growth and organ development of broiler chicks. WSP50 could increase the growth performance of broilers fed a corn-soybean meal-based diet.

• Korean Journal of Medicinal Crop Science
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• v.10 no.1
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• pp.24-28
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• 2002

Effect of pretreatment processing (peeling time, drying temperature, sample type) on the quality of hot air drying peony roots was investigated. Peony roots were peeled for 0, 10, 20, 30, 60, 90 and 120 minute in the peeling machine after water washing. Sample types were whole or cut (thickness of 3mm). Temperatures of hot air drying were 30, 40, 50 and $60^{\circ}C$ The level of water contents in dried sample was $14{\sim}15%$. Drying time was muth reduced as the peeling time, cutting sample types and higher temperature. Contents of paeoniflorin were maintained highest amount (3.48%) in the non-peeling cut sample dried at $30^{\circ}C$ and lowest amount (2.29%) in the 120min-peeling whole one dried at $60^{\circ}C$. Skin color of dried peony roots was heavily discolored in the sample of non-peeling whole type dried at $60^{\circ}C$ and the color was dark brown $({\Delta}E\;was\;46.1)$. When considering shortening of drying time, preservation of active compounds and maintenance of color, the best pretreatment method of drying in peony roots was non-peeling, cut type processing.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.6
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• pp.387-394
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• 2002

This study was conducted to compare an econamical efficiency of a recommended rate of fertilizer with the criterion of the sustainable agriculture direct income support system(SADISS). Red pepper was cultivated with different fertilizer application levels of the standard amount of fertilizer for red pepper(SAFR), fertilizer recommended by soil testing for red pepper(FRST) and decrement of 20, 40, 60 and 80 percent of fertilizer recommended by soil testing for red pepper(DFRST 20, 40, 60, 80) and that of farmer's habitual practies fertilization (FHPF). The changes by different levels of applied fertilizer was compared with those of SADISS. The results were summirized as follows: No yield difference was found among the FHPF, SAFR, FRST and DFRST20, but yield decrease in FRST40, 60 and 80. T-N, $P_2O_5$ and $K_2O$ in FHPF were 24, 9 and 53 percent respectively, and it were the lowest ones among the treatments. Fertilizer efficiency of red pepper was decreased with application rate of fertilizer. Fertilization ratio of N and P in DFRST(40, 60, 80 percent) was not sufficiency for red pepper. Economical efficiency of FRST and DFRST20 showed sufficiently in comparison with FHPF. Therefore, it could be possible that FRST and DFRST20 were recommended as methods for SADISS in red pepper cultivation.

• Korean Journal of Environmental Agriculture
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• v.1 no.2
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• pp.116-122
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• 1982

Investigation on the water quality of Hwangguji River and experiment on the effects of nitrogen and soil improvements were carried out in the paddy field irrigated with polluted water of the river. The obtained results are as follows: 1) Amount of COD and $NH_4-N$ in water of the river were 54 ppm, 65 ppm, during the seeding time, and were 52 ppm, 512 ppm during the transplanting time respectively. Their concentrations were over the standard levels. It seemed that the water pollution was mainly caused by organic waste matters. 2) It seemed that the effective nitrogen level was $7{\sim}8$kg/10a in the paddy field irrigated with polluted water of the river. 3) The rice yields of potassium twice quantity application plot with N.P.K. fertilizer, the calcium application plot with N.P.K. fertilizer and the combined plot with potassium, wallarstonite, calcium and fresh straw, were increased 4, 5 and 8%, respectively, than that of the N.P.K. fertilizer standard level plot.