• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.335-341
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• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

• 한국정보과학회논문지:시스템및이론
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• 제32권9호
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• pp.465-474
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• 2005

고집적 SoC 설계시에 버스방식의 온칩 통신은 대역폭이 제한되는 문제점이 있고 NoC (Network-on-Chip) 방식에서는 구현의 복잡도가 증가하는 문제점이 있다. 본 논문에서는 이러한 문제점을 극복하는 새로운 온칩 통신 규격인 SNP(Soc Network Protocol)를 소개한다. SNP는 기존 버스의 신호선들을 세 가지 그룹인 제어(control), 주소(address), 데이타(data)로 나눈 뒤 하나의 채널을 통해 전송함으로써 신호선의 수를 줄인다. SNP 채널은 대칭구조로 사용되기 때문에 마스터-슬레이브 통신 방식뿐만 아니라 마스터-마스터 통신도 효율적으로 지원한다. 하나의 전송에 필요한 신호 그룹의 진행 규칙을 SNP 규격으로 정의하고, 동일한 정보가 반복적으로 전달되는 것을 방지하는 페이즈 복원 기능을 제안하여 통신대역을 효율적으로 사용할 수 있도록 한다. 산업계 표준 규격인 AMBA AHB와 비교한 결과 멀티미디어 타입의 데이타 전송시에 $54\%$의 신호선수만으로도 대등한 대역폭을 지원할 수 있음을 보인다.

• 한국음향학회지
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• 제33권1호
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• pp.75-80
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• 2014

실시간 VoIP 네트워크는 지연, 지터 그리고 패킷손실과 같은 네트워크 장애요소로 인해 품질저하가 발생한다. 본 논문은 VoIP 음질 향상을 위해 잡음환경에 강인한 음성분류기반의 패킷손실 은닉 알고리즘을 제안한다. 제안된 방식에서는 음성신호로부터 추출된 다양한 특징들을 분석하고 이를 기반으로 획득된 적응적인 문턱값을 사용하여 수신단에 도착한 패킷을 분류한다. 정확한 신호분류 결과는 패킷손실 은닉에 사용된다. 그리고 선형 예측 기반의 손실패킷 은닉은 연속적으로 패킷을 은닉하거나 손실된 패킷복원 시 발생하는 메탈릭 아티펙트를 제거함으로써 고품질의 음성을 제공한다.

• Journal of Communications and Networks
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• 제10권3호
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• pp.351-361
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• 2008

This paper presents a delay-margin based traffic engineering (TE) approach to provide end-to-end quality of service (QoS) in multi-protocol label switching (MPLS) networks using differentiated services (DiffServ) at the link level. The TE, including delay, class, and route assignments, is formulated as a nonlinear optimization problem reflecting the inter-class and inter-link dependency introduced by DiffServ and end-to-end QoS requirements. Three algorithms are used to provide a solution to the problem: The first two, centralized offline route configuration and link-class delay assignment, operate in the convex areas of the feasible region to consecutively reduce the objective function using a per-link per-class decomposition of the objective function gradient. The third one is a heuristic that promotes/demotes connections at different links in order to deal with concave areas that may be produced by a trunk route usage of more than one class on a given link. Approximations of the three algorithms suitable for on-line distributed TE operation are also derived. Simulation is used to show that proposed approach can increase the number of users while maintaining end-to-end QoS requirements.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제11권3호
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• pp.216-223
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• 2005

컴퓨터 클럭은 자체의 불안정한 요소, 물리적 특성, 외부 환경의 요인, 사용자의 개입 및 시스템의 오류 요소로 인해 정확도와 안정도에 한계를 갖는다. 따라서 정밀한 시각 관련 처리가 필요한 시스템의 경우 표준 시각에의 동기화가 요구된다. 시각 동기의 목적은 분산 처리 시스템 상에 전역 시각 기저를 제공하는데 있다. 일단 이러한 시각 기저가 존재하게 되면, 분산 시스템 상에서 처리되는 일련의 작업들은 시각을 바탕으로 제어가 가능하기 때문이다. 본 논문은 인터넷 기반 분산 환경에서 시각 동기를 위한 임베디드 시스템의 설계 및 구현에 대해 제시한다. 시스템은 참조 시각원으로 GPS(Global Positioning System)를 사용하고, NTP(Network Time Protocol)를 통해 표준시(UTC: Universal Time Coordinated)를 제공함으로써 분산 시스템의 시각 동기를 이룬다. 이를 위해 안정적인 시각 유지 및 정확하고 정밀한 표준 시각 제공 요건을 만족하는 클럭 모델을 설계, 적용하였다. 네트워크 관리를 위해SNMP(Simple Network Management Protocol) 기반 private MIB(Management Information Base)를 정의하였고, 전체 시스템의 구현 및 성능 평가 결과도 제시하였다.

• 한국정보처리학회논문지
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• 제6권3호
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• pp.729-741
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• 1999

ATM networks are widely deployed as the network that is capable of supporting multimedia services efficiently now. To date, a large portion of ATM connections, particularly in the WAN environment, have been of a permanent virtual circuits-requiring management intervention for set-up and tear-down. However, switched virtual circuits are required to support a range of desired characteristics on demand, to a reachable end user. To establish, maintain and release on-demand call/connections, the user-network interface(UNI) and node-node interface(NNI) signalling capabilities are required. Two protocols have been specified for NNI signalling within a public network: the broadband integrated-services user part(B-ISUP) protocol specified by the ITU-T, and the private network-network interface(PNNI) protocol specified by the ATM Forum. PNNI offers different type of internetwork or internodal interface from the traditional B-ISUP approach favored to date public network operators. In spite of its name, PNNI may find its place in network service provide networks as well as in private networks. Thus many public network operators and ATM equipment manufacturers are still unable to choose the NNI interface architecture of their system. In this paper, we survey the characteristics of the B-ISUP and PNNI protocols, and investigate the applicability issue of these specifications to the ATM-based Information Infra-Network of Korea. Analyzing the characteristics of the two protocols and clarifying the NNi requirements of the ATM-based Information Infra-Network of Korea, we propose that the B-ISUP protocol is more suitable than PNNI.

• 방송공학회논문지
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• 제23권5호
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• pp.682-692
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• 2018

본 논문에서는 MMT(MPEG Media Transport) 프로토콜 기반의 대용량 멀티미디어 전송에서 다중쓰레드를 활용한 ARQ 패킷 오류 제어 기법을 제안한다. 송신 측에서는 영상을 구성하는 각 프레임을 MMT 프로토콜을 기반으로 패킷 단위로 잘라 패킷의 헤더(Header)에는 패킷이 포함된 프레임의 순서, 표현 시간 정보 등을 저장하고 페이로드(Payload)에는 프레임을 구성하는 직접적인 정보를 저장하여 IP(Internet Protocol) 망으로 전송한다. 수신 측에서는 수신한 패킷의 오류 발생 여부를 판단하여 오류가 발생한 경우 재전송을 통해 오류를 제어하고 수신한 패킷의 헤더에 저장된 정보에 따라 패킷을 프레임으로 재구성한다. 이때 다중쓰레드 기반의 전송 방식을 설계 및 적용하여 각 쓰레드가 하나의 프레임을 맡아 패킷화(packetization)하고 전송함으로써 대용량 멀티미디어의 전송 효율을 높인다. 또한 오류가 발생한 패킷을 재전송 할 경우 단일쓰레드를 사용할 때 나타날 수 있는 문제점을 해결함으로써 다중쓰레드 전송 방식의 효율성을 검증한다.

• International journal of advanced smart convergence
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• 제4권1호
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• pp.18-30
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• 2015

As the speed of networks that subscribers can use has greatly increased, demand for high-quality broadcast content, such as from Internet Protocol Television (IPTV) and Video on Demand (VoD), is likewise increasing. Therefore, while broadcasters are increasing content and channels, they are striving to improve consumer quality of experience (QoE) to differentiate themselves from competitors, including by producing higher physical-quality content. Recently, subjective measurement methods have been internationally standardized as the most reliable approach for measuring and evaluating IPTV QoE. However, a majority of these methods are performed in experimental environments and are based on the extremely brief viewing period of approximately ten seconds using original reference videos. It is actually difficult to apply standard evaluation methods based on a ten-second viewing interval to assess real broadcast watching of IPTV or other services that involve a longer time (i.e., more than thirty minutes). In this paper, we therefore propose a method that accommodates actual viewing environments. Using the mean opinion score, we experimentally analyze the effects of evaluation interval changes under actual conditions in which IPTV service is provided. In addition, we propose improvements by applying the results into actual live broadcast IPTV service and by analyzing consumer service QoE.

• Journal of Computing Science and Engineering
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• 제4권3호
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• pp.225-239
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• 2010

This paper introduces a new approach to modeling relative distance among nodes under a variety of communication rates, due to node's mobility in Mobile Ad-hoc Networks (MANETs). When mobile nodes move to another location, the relative distance of communicating nodes will directly affect the data rate of transmission. The larger the distance between two communicating nodes is, the lower the rate that they can use for transferring data will be. The connection certainty of a link also changes because a node may move closer to or farther away out of the communication range of other nodes. Therefore, the stability of a route is related to connection entropy. Taking into account these issues, this paper proposes a new routing metric for MANETs. The new metric considers both link weight and route stability based on connection entropy. The problem of determining the best route is subsequently formulated as the minimization of an object function formed as a linear combination of the link weight and the connection uncertainty of that link. The simulation results show that the proposed routing metric improves end-to-end throughput and reduces the percentage of link breakages and route reparations.

• 한국지능시스템학회논문지
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• 제24권2호
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• pp.209-217
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• 2014

Of several approaches for future Internet, separating two properties of IP address into locator and identifier, is being considered as a highly likely solution. IETF's LISP (Locator ID Separation Protocol) is proposed for this architecture. In particular, the LISP model easily allows for device mobility through simple update of information at MS (Mapping Server) without a separate protocol. In recent years, some of the models supporting device mobility using such LISP attributes have emerged; however, most of them have the limitation for seamless mobility support due to the frequent MS information updates and the time required for the updates. In this paper, PMIPv6 (Proxy Mobile IPv6) model is applied for mobility support in LISP model. PMIPv6 is a method that can support mobility based on network without the help of device; thus, this we define anew the behavior of functional modules (LMA, MAG and MS) to fit this model to the LISP environment and present specifically procedures of device registration, data transfer, route optimization and handover. In addition, our approach improves the communication performance using three tunnels identified with locators between mobile node and corresponding node and using a route optimized tunnel between MN's MAG and CN's MAG. Finally, it allows for seamless mobility by designing a sophisticated handover procedure.