• Food Science and Preservation
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• v.21 no.6
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• pp.808-814
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• 2014

The nutrient contents of pumpkins depend on many factors, including the species, climate, soil type, and plant part. The nutritional compositions of five pumpkin cultivars (red boujjang, mini hong, mini gold, boujjang, and yakhobak) were investigated. To analyze the nutrient contents of the edible parts, the other parts (seeds, skin, and stem) of each pumpkin were removed. The results showed that the proximate compositions of the edible parts of the pumpkins ranged from 74 to 87 g per 100 g for moisture, 1.6 to 3.2 g per 100 g for crude protein, 10.1 to 20.7 g per 100 g for carbohydrate content, and 2.0 to 3.4 g per 100 g for total dietary fiber. The proximate contents of the pumpkin samples were not significantly different. The thiamine contents of the pumpkins, except for red boujjang, varied from 0.03 to 0.15 mg per 100 g, but that of red boujjang had the highest level (0.60 mg per 100 g). The vitamin C contents of the pumpkins, except for boujjang, ranged from 13.0 to 14.0 mg per 100 g, but that of boujjang was the highest (49.0 mg per 100 g). In particular, yakhobak showed the highest total carotenoid and folate levels (279.5 and $74.0{\mu}g$ per 100 g, respectively). These results will be useful for coming up with better pumpkin varieties through breeding, and established the nutrient compositions of pumpkins.

• Journal of Life Science
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• v.25 no.10
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• pp.1148-1155
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• 2015

Commercial complete feeds contain enough nutrients to support animal growth and it is easy to be spoiled under proper temperature and humid conditions. The aim of this study was to investigate microbiological and chemical changes on complete feed for milking cow under open-air exposure with moisture 33% at 30℃ during 15 days. pH decreased 6.29 to 4.66 and water activity decreased gradually 0.99 to 0.95. Bacteria increased 6.2×10⁶~1.6×10⁷ to 2.1×10⁹ CFU/g at 5 days and showed 10⁸ CFU/g until 15 days. Fungi increased 10³ CFU/g to 8.0×10⁴ CFU/g. During the processing of spoilage, bacteria such as Acinetobacter oleivorans, Pediococcus acidilactici, Acinetobacter oleivorans, Weissella cibaria, and Methylobacterium komagatae were identified and fungi such as Fusarium sp. and Mucor sp. were also identified. Moisture content increased until 10 days (p<0.01). Crude protein was not changed so much whereas crude fat decreased 6.0% to 5.5% (p<0.01). Crude fiber and crude ash changed 2.0~ 3.0% and 4.5~ 4.8% levels with no significance, respectively. Gross energy was not almost changed at 4,400 kcal/g. During spoilage, lactate and propionate increased whereas acetate was not detected. Protease and lipase activities increased significantly during spoilage (p<0.01). Zearalenone content increased 59.2 μg/kg to 623.8 μg/kg, showing 10.5 times more production. During feed spoilage, pH decreased with microbial growth and various chemical changes were occurred.

• Journal of Applied Biological Chemistry
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• v.59 no.3
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• pp.179-188
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• 2016

This study is aimed to evaluate the protective effect of Gastrodiae rhizoma and steamed, dried & fermented Gastrodiae rhizoma on Lipopolysaccharide (LPS)-induced hepatic injury in the mice model. Sample was selected to GR0F0 (not processed gastrodia rhizome) and GR6F4 (fermented with Saccharomyces cerevisiae before steamed and dried 6 times) based on 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid, and High-performance liquid chromatography analysis. Mice were randomly divided into 4 groups - Normal group, vehicle group (LPS treated), GR0F0 group (fed GR0F0 before LPS treated) and GR6F4 group (fed GR6F4 before LPS treated) with 6 mice in each group. GR0F0 group and GR6F4 group were fed each extract 200 mg/kg/day during 8 days. LPS 20 mg/kg injected to the experimental groups as abdominal injection. We measured aspartate aminotransferase, alanine amino-transferase in serum. GR0F0 and GR6F4 showed a significant decrease compared to the vehicle group. As a result of measuring the ROS, GR6F4 group showed a significant reduction in both the serum and liver tissues compared to the vehicle group. GR0F0 group showed a significant reduction only in the liver tissues. Activator protein-1, cyclooxygenase-2, and Inducible nitric oxide synthase were significantly decreased GR0F0 group and GR6F4 group. But tumor necrosis factor alpha only showed a significant reduction in GR6F4 group. GR0F0 and GR6F4 groups against liver damage in mice with LPS. That showed significant effects on anti-oxidant and anti-inflammatory action. The effects of GR6F4 group showed superior results compared to GR0F0 group. Therefore, Steamed, dried & fermented Gastrodia rhizoma was might have a protective effect on liver injury.

• Korean Journal of Poultry Science
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• v.36 no.1
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• pp.69-75
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• 2009

The effects of different packaging methods such as aerobic, vacuum, and wrap packaging, on quality characteristics of fresh chicken breast meat during cold storage were investigated. The results show that crude fat content in aerobic packaging method was significantly (P<0.05) higher compared to the others, whereas moisture, protein and ash contents were not significantly different. pH in wrap packaging was increased with storage time and reached the highest values at 10 days. Both lightness and redness of the meat were increased with storage time, but lightness was significantly (P<0.05) higher in wrap packaging than in the others. Thiobarbituric acid (TBA) and volatile basic nitrogen (VBN) values were increased in all treatments at 10 days. VBN at 5 days were over 20 mg%, and TBA values at 10 days were between 0.82~1.05 mg malondialdehyde/kg meat. TBA showed significantly (P<0.05) lower in vacuum packaging compared to the other methods. Therefore, our results suggested that vacuum packaging decreases lipid oxidation of chicken breast meat, thereby enhancing the shelf life, compared to aerobic and wrap packaging methods.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.177-186
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• 2006

This study was conducted to investigate the effects of the types and rates of application of animal manure on productivity of silage corn and environmental pollution in silage corn cultivation soil. The experiment was confirmed in lysimeter which was constructed with 0.30m diameter, and 1 m depth. This study was arranged in split plot design. Main plots were the types of cattle slurry (CS), swine manure fermented with sawdust (SMFS) and chemical fertilizer (CF), Subplots were the application rates of animal manure, as urea, such as 100, 200 and 400 kg N $ha^{-1}$. Dry matter(DM) and nitrogen yields of silage corn enhanced as increased application rates of CS, SMFS and CF (p<0.05). DM yield reveals that there is an decrease in order of CF>CS>SMFS (p<0.05). Crude protein (CP) contents of the whole silage corn increased as increased application rates of CS, SMFS and CF. IN addition, $NO_{3^-}N$ content in leaching water by application of animal manure reveals that there is an decrease in order SMFS>CF>CS (p<0.05). However, $NH_{4^-}N$ content was hardly influenced by application of animal manure, and $NH_{4^-}N$ content increased with application rates increased. $PO_{4^-}P$ content in leaching water by application of animal manure reveals that there is an decrease in order of SMFS>CF>CS. $PO_{4^-}P$ increased as increasing application rates (p<0.05), whereas $PO_{4^-}P$ in leaching water maintained a low levels.

• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.2
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• pp.71-77
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• 1986

The field experiment was conducted to evaluate the effect of herbicide treatment (Banvel: 100, 200, U-46: 150, 300, Hedonal: 150, 300, Simazin: 100, 200g/10 a) on change in the botanical composition and dry matter productivity of ladino clover (Trifolium repens L.) dominated pasture mixtures. The experiment was laid down as a randomized block design with 4 replications at experimental field of Livestock Experiment Station in Suweon from 1983 to 1985. The results obtained are summarized as follows: 1. Against ladino clover in mixie grass-clover swards Banvel, U-46 and Hedonal gave good control. The percentage of ladino clover under herbicide tratment decreased to about 1-2%(Banvel), 11-18%(U-46) and 22-31%(Hedonal), respectively, while it increased to 71% in untreated pastures. However, in the second year this trend stated to reverse and occurred clover dominance again in U-46 and Hedonal application, while those remained approximately constant until end of the years under Banvel treatment. Simazin is to be not recommended. 2. The best time for herbicide treatment was found to be late summer before autumn sown 20-25 days. When it applied in early summer weed infestigation by Digitaria spp., Echinochloa spp. and other species was a severe problem. 3. Emergence and early development of introduced pastures were less satisfactory, if it oversown immediatley after herbicide treatments due to its phytotoxical damage. Residual chemicals remained about 7-10 days in topsoils. Perennial ryegrass and orchardgrass were slightly less tolerant than the other species. 4. In dry matter, taken as average of three year results, Banvel applied pastures produced the remarkably high yield of 1023 kg/10 a, which is as much as 44% higher than that of untreated plot. Dry matter yields under U-46 and Hedonal treatment were 842 and 811 kg/10 a, respectively. 5. Weender components and net energy concentration were affected by change in the botanical composition. Crude protein and NEL value were slightly higher in clover dominance than those in herbicide treatments. Total yields of net energy lactation, however, were the highest in Banvel application with 5401 MJ and the lowest in untreatment with 3889 MJ-NEL/10 a DM.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.3
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• pp.155-160
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• 2007

This experiment was conducted to compare the agronomic characteristics and productivity in induced vetch cultivars at paddy field of Cheonan (National Institute of Animal Science) and Naju (Jeollanam-Do Agricultural Research and Extension) from 2004 to 2006. The vetch cultivars used in this study were 6 varieties ('VV4712', 'Welta', 'Penn-02', 'Ostsaat', 'Oregon com.', 'Sander 2'). At the Naju region, the most fast flowering date variety was Chinese milk vetch, Sander 2 was very late as 20th May. The average dry matter (DM) content was 21.7%. Vetch cultivars were severely different. 'Oregon com.' showed the highest DM yield by 5,406 kg/ha but the DM yield of 'Ostsaat' was low by 4,852 kg/ha. At the Cheonan region, the most fast flowering date variety was Korean milk vetch, Sander 2 was very late as 25th May. The average dry matter (DM) content was 16.9%. Vetch cultivars were severely different. 'Welta' and 'Ostsaat' showed the highest DM yield by 2,173 kg/ha and 1,883 kg/ha respectively, but the DM yield of 'VV4712' was low by 1,079 kg/ha. Crude protein (CP) content of vetch was 11.5%. 'Sander 2' showed the highest CP content by 14.4% and Chinese milk vetch showed the highest content by 19.3%. Average acid detergent fiber (ADF), neutral detergent fiber (NDF) and total digestible nutrient (TDN) content were 26.8%, 42.2% and 67.5%, respectively. The results of this experiments indicated that the productivity of vetch cultivar varied from winter hardness, therefore we will consider the winter hardness as main factor when induce vetch cultivars. According to the results obtained from this study, it is suggested that 'Oregon com.' at the Naju region and 'Welta' and 'Ostsaat' at the Cheonan region would be recommendable for fall sown 'vetch' for dry matter production.

• Applied Biological Chemistry
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• v.17 no.1
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• pp.63-80
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• 1974

The chemical components of Citrus junos produced in Korea were divided into two parts; common and special components respectively. In the former the relation between the physiological effects of the plant and its ripening process was observed periodically while the latter was analyzed the ripening fruits for their effective utilization as food. The results are summarized as follows: 1. The analytical result of seasonal change showed that the rind ratio was higher than the flesh ratio and on a regional basis, the rind ratio was higher in the islands than on land areas. 2. In the experiment the moisture was increased until the third period, but afterwards it was made constant. While the content of crude fat, cellulose, ash, total acid and soluble non-nitrogen material were decreased until the third period and the cotent of cellulose and total acid were continuousely redused until the last period. In con trast with the above the content of reducing sugars was increased but the content of crude fat, cellulose, ash, crude protein and soluble non-nitrogen material were increased until last period. 3. The content of vitamin C was richer in the rind than in the flesh, in the Korean species than in the Japanese. 4. Free sugars; xylose, fructose, glucose were richer in the rind than in the flesh. 5. The content of volatile organic acids was richer in the rind than in the flesh. Among them, volatile acids, acetic acid, formic and n-valeric acid were found in the rind and formic acid, acetic acid and propionic acid were deteceed in the flesh. 6. The total content of non-volatile acids was richer in the flesh than in the rind. In the kind of non-volatile acids, citric acid,glutaric acid, malic acid, tartaric acid, oxalic acid, malonic acid, succinic acid and an unknown acid were found in the rind and citric acid, malic acid, succinic acid, oxalic acid, glutaric acid and malonic acid in the flesh. 7. Three kinds of aromatic components: D-limonene, ${\alpha}-pinene$, p-cymene and seven other kinds of unknown aromatic components were detected in neutral essential oils. Among them, D-limonene seemed to be main aromatic component in the fruits. 8. From the above results it is confirmed that both rind and flesh of the ripened fruit could be utilized for food effectively, and unripened fruits are suitable for producing citric acid, ripened fruits are also useful for producing juice.

• The korean journal of orthodontics
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• v.28 no.5 s.70
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• pp.699-716
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• 1998

This study was designed to evaluate the expression of non-collagenous protein in periodontal tissue during the experimental movement of rat incisors, by LSAB(labelled streptavidine biotin) immunohistochemical staining for osteonectin and osteocalcin. Twenty seven Sprague-Dawley rats were divided into a control group(3 rats) and 6 experimental groups(24 rats) where 75g of force was applied from helical springs across the maxillary incisors. Rats of experimental groups were sacrificed at 12 hours, 1, 4, 7, 14 and 28 days after force application, respectively. And the tissues of a control group and experimental groups were studied immunohistochemically and histologically. The results were as follows : 1. Until 28 days after force application, periodontal fibers had been strectched on tension side and compressed in pressure side of all the experimental groups, and the arrangement of periodontal fibers had not been recovered yet. 2. The expression of osteonectin in control group was rare in dentin, cementum and osteocyte, and was mild in odontoblasts and matrix of alveolar bone. 3. The expression of osteocalcin in control group was negative in gingiva, osteoblasts, osteocyte and cementum, and was rare in predentin, capillaries in pulp and periodontal ligament and the matrix of alveolar bone. 4. There was no difference in the expression of osteocalcin or osteonectin in dentin, cementum, pulp, odontoblasts, between of control and of experimental groups. 5. The expression of osteonectin in intermaxillary suture got the peak in 7-day and was declined after 14-day. The expression of osteocalcin remained in a same degree since it became mild in 14-day. 6. The expression of osteonectin in pressure side of periodontal ligament of experimental group was rare, which was similar to control group. But in tension side, it was increased until 14-day aftrer which it was declined. 7. The expression of osteocalcin in periodntal ligament was rare in 12-hour to 14-day, but became severe in 28-day, which was greater in tension side than in pressure side, and in the periodontal fiber next to alveolar bone than to tooth surface. 8. The expression of osteocalcin in alveolar bone was rare until 14-day in pressure side, but became moderate in 28-day. The expression of osteonectin was increased from 7-day by time dependency, which was greater in tension side than in pressure side.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.6
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• pp.920-926
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• 2010

Marine alga, Chlorella vulgaris, was extracted by chloroform-methanol (2:1, v/v) solvents for lipid extraction at $35^{\circ}C$ for five hours (HCM-35) and its process was compared with conventional lipid extraction condition such as chloroform-methanol (2:1, v/v) at $65^{\circ}C$ for one hour (CM-65). This low temperature extraction process showed that 80% of total lipid was extracted and its residues contained relatively unchanged amounts of intact proteins and other minerals as well as amino acid profiles. Interestingly enough, the weight fraction of carbohydrate in the residues slightly increased due to less denaturation at low process temperature. The biological activities of the residues such as cytotoxicity and immune cell growth activation were not much changed after being extracted. The sensory evaluation were found to be very favorable for being used as a food additive and/or food supplement. This result could also help to maintain the economic feasibility of utilizing marine resources in food and other relevant industries.