• 제목/요약/키워드: Protein requirement

검색결과 354건 처리시간 0.026초

Quantitative Analysis of Protein-RNA Interaction in A Class I tRNA Synthetase by Saturation Mutagenesis

  • Kim, Sung-Hoon
    • BMB Reports
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    • 제28권4호
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    • pp.363-367
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    • 1995
  • E. coli methionyl-tRNA synthetase is one of the class I tRNA synthetases. The Tryptophane residue at the position 461 located in the C-terminal domain of the enzyme is a key amino acid for the interaction with the anticodon of $tRNA^{Met}$. W461 was replaced with other amino acids to determine the chemical requirement for the interaction with the anticodon of $tRNA^{Met}$. Saturation mutagenesis at the position 461 generated a total of 12 substitution mutants of methionyl-tRNA synthetase. All the mutants showed the same in vivo stability as the wild-type enzyme, suggesting that the amino acid substitutions did not cause severe conformational change of the protein The mutants containing tyrosine, phenylalanine, histidine and cysteine substitutions showed in vivo activity while all the other mutants did not. The comparison of the in vitro aminoacylation activities of these mutants showed that aromatic ring structure, Van der Waals volume and hydrogen bond potential of the amino acid residue at the position 461 are the major determinants for the interaction with the anticodon of $tRNA^{Met}$.

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Proteomic Analysis of Circadian Clock Mutant Mice

  • Lee Joon-Woo;Kim Han-Gyu;Bae Kiho
    • 대한의생명과학회지
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    • 제11권4호
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    • pp.493-501
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    • 2005
  • Circadian rhythms, time on a scale of about 24 hours, are present in a number of organisms including animals, plants, and bacteria. The control of the biochemical, physiological and behavioral processes is regulated by endogenous clocks in the suprachiasmatic nucleus (SCN). At the core of this timing mechanism is molecular machinery that are present both in the brain and in the peripheral tissues throughout the body, and even in a single cultured cell. In this study, we performed two-dimensional gel electrophoresis to figure out any correlation between protein expression patterns and the requirement of two canonical clock proteins, either mPER1 or mPER2, by comparing global protein expression profiles in livers from wildtype or mPer1/mPer2 double mutant mice. We could identify several differentially expressed protein candidates with respect to time and genotypes. Further analysis of these candidate proteins in detail in vivo will lead us to the better understanding of how circadian clock functions in mammals.

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Effects of Dietary Energy Level and Feeding Ration on Growth and Body Composition of Nile Tilapia, Oreochromis niloticus (L.)

  • Cho, Sung-Hwoan;Jo, Jae-Yoon
    • 한국어업기술학회:학술대회논문집
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    • 한국어업기술학회 2000년도 춘계수산관련학회 공동학술대회발표요지집
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    • pp.271-273
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    • 2000
  • Recently, possibility of low protein diet with high energy level for improvement of fish production has been proposed in several fish species, especially under restricted feeding rate, fish responded better on high energy diet than low energy diet McGoogan and Gatlin (1999) stressed another benefit of low protein diet with high energy for fish production, in terms of reduction of nitrogen waste from fish metabolism However, others reported no effect of high lipid in feed on performance of fish (Jover et al. 1999). This was probably because that energy availability o requirement for fish varied based on fish species, protein content or quality in feed, rearing water temperature condition and feeding ration. (omitted)

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자유로운 식이와 활동을 유지하는 한국 여대생의 에너지와 단백질 대사에 대한 연구(1) : 에너지 섭취와 평형 (Nutritional Status and Requirements of Protein and Energy in Female Korean College Students Maintaining Their Usual Diet and Activity(1) : Energy Intake and Balance)

  • 김주연
    • Journal of Nutrition and Health
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    • 제27권4호
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    • pp.336-346
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    • 1994
  • balance and to estimate daily energy requirement in 43 Korean female college students maintaining their usual diet and activity level. Energy intake and expenditure were measured in two separate periods about one month apart, each period lasting for 3 days. All the subjects participated in both periods. Energy intake was assessed by two methods ; weighed diet record and duplicate portion analysis of diet minus fecal and urinary excretion. Mean daily energy intake level calculated from diet records was 28.5 kcal/kgB.W(1, 476 kcal/day), and similar to the level of 27.8 kcal/kgB.W(1, 438 kcal/days) obtained from the chemical analysis of duplicate portions. Mean daily energy expenditure, calculated from activity records of each subject, was 34.6 kcal/kgB.W, or 1.39 times BMR, which corresponds to light activity level. Mean daily energy balance of subjects was -5.9 kcal/kgB.W. Mean daily requirement of energy, calculated from the activity records of the subjects, was 34.6 kcal/kgB.W, similar to the level of the Korean RDA for light activity level. The results of this study indicate that 1) the activity level of the study subjects corresponds to the sedentary level ; 2) present Korean RDA for energy for light activity is adequate for the subjects ; and 3) the subjects should increase their energy intake since they are largely in negative energy balance.

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Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권12호
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).

사료 내 단백질 함량 및 합성아미노산 첨가에 따른 육계의 사양성적 및 가슴육 성상에 미치는 영향 (Effects of Reduced Dietary Crude Protein Diet with Synthetic Amino Acid Supplements on the Growth Performance and Histochemical Characteristics of the Breast Meat of Male Broilers)

  • 안수현;김관응;안병기;공창수
    • 한국가금학회지
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    • 제49권1호
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    • pp.33-43
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    • 2022
  • The present study was conducted to determine the effect of reduced dietary crude protein (CP) with a synthetic indispensable amino acids (AA) supplement on the growth performance, economics, and breast meat quality. A total of 450 male broilers (Ross 308) were used from the age of 7 to 28 days. On d 7, the birds were individually weighed and randomly assigned to three treatment diets with six replicate pens for each treatment in a randomized complete block design. The experimental diets were: (1) a control diet, (2) a diet with a reduction of 1% of the dietary CP with synthetic AA supplements to meet the indispensable AA requirement (OAA) of broiler chickens, and (3) a diet with a reduction of 1% of the dietary CP with synthetic Lys, Met, and Thr supplementation to exceed 5% of the indispensable AA requirement (HAA). There were no differences among the treatments on the final body weight, weight gain, and feed intake. However, the HAA diet impaired the feed conversion ratio at d 21 and during the overall feeding periods (P<0.05). The predicted total feed intake and feed price required to reached 1.5 kg BW was higher than for the birds fed the HAA diet (P<0.05). The breast meat muscle fiber cross sectional area and fiber density varied between the treatments (P<0.05). However, there were no differences in breast meat weight. In conclusion, reducing 1% of dietary CP had no adverse effects on the growth performance or breast meat yields.

Recombinant human BMP-2/-7 heterodimer protein expression for bone tissue engineering using recombinant baculovirus expression system

  • Park, Seung-Won;Goo, Tae-Won;Kim, Seong Ryul;Choi, Kwang-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • 제32권2호
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    • pp.49-53
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    • 2016
  • Bone morphogenetic proteins (BMPs) are essential growth factors for bone formation, skeletal development and bone regeneration. The BMP-2/7 heterodimer is known to have remarkable effects on osteogenic induction that are even stronger than the BMP-2 or BMP-7 homodimers. We designed a recombinant human BMP-2/7 (rhBMP-2/7) heterodimer protein with four glycine residues between BMP-2 and BMP-7 protein to facilitate free bond rotation of domains. The Baculovirus Expression Vector System (BEVS) is routinely used to produce recombinant proteins in the milligram scale. In this study, the BEVS was used to express the rhBMP-2/7 protein whrer the recombinant baculovirus was recovered in the host Sf9 cells. To confirm the biological activity of rhBMP-2/7 protein secreted from the BEVS as an osteogenic differentiation and induction factor, we measured the BMP-induced ALP activity. rhBMP-2/7 could be used as an alternative to BMPs to overcome limitations like short half-life and requirement for high concentrations. Furthermore, rhBMP-2/7 may be an efficient tool for various application studies such as bone regeneration and skeletal development.

유색육용계의 성장과 혈액성상에 사료단백질 및 에너지가 미치는 영향 (Effect of Dietary Energy, Protein on Growth and Blood Composition of Cross Bred Chicks)

  • 정용대;류경선
    • 한국가금학회지
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    • 제35권3호
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    • pp.291-302
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    • 2008
  • 국내에서 사육되는 유색육용계 성장에 적합한 에너지와 단백질 수준을 구명하여 사료 급여 체계 확립에 필요한 기초자료를 제공하고자 두 차례의 사양 실험을 수행하였다. 공시동물은 유색육용계 1일령 암, 수컷 1,404수를 사용하였고, 펜당 26수씩 배치하였다. 사료내 에너지 수준은 사육전기 5주간 3,000, 3,100 kg/kcal이며, 단백질 함량은 $0{\sim}2$주에 20, 21, 22%를 급여하였으며, $3{\sim}5$주에 18, 19, 20, 21, 22%로 처리하였다. 조사 항목은 체중, 사료 섭취량을 측정하여 생산성을 계산하였으며, 실험 종료시 혈액을 채취하여 분석하였다. 실험 1 : $0{\sim}2$주간 사료 ME 함량 차이에 따른 생산 능력은 처리구간에 차이가 없으며 CP 함량 차이에 기인한 증체량과 사료 섭취량도 차이가 없었지만 사료 요구율은 CP 21, 22% 처리구에서 CP 20% 처리구에 비하여 통계적으로 개선되었다(P<0.05). 실험 개시후 $3{\sim}5$주간에 사료 ME 3,100 kcal/kg 처리구에서 ME 3,000 kcal/kg 처리구에 비하여 사료 요구율은 개선되었다(P<0.05). 이 기간에 사료내 CP 22% 처리구는 낮은 수준의 처리구보다 증체량이 유의하게 증가하였고(P<0.05) 사료 요구율은 CP가 높은 수준의 사료를 급여시에 낮은 수준의 CP 처리구에 비하여 개선되었지만 사료 섭취량은 처리구간에 차이가 없었다(P<0.05). 이 기간에 증체량과 사료 요구율은 ME와 CP간 상호 작용하였다(P<0.05). 전 실험기간 5주간에 사료 요구율은 ME 3,100 kcal/kg 처리구에서 ME 3,000 kcal/kg 처리구보다 개선되었으며, CP (20)18% 처리구는 다른 처리구에 비하여 높게 나타났다(P<0.05). 증폐량은 CP (22)22% 처리구에서 CP (21)21%, (20)18% 처리구보다 증가하였으며, ME와 CP간에 상호 작용은 증체량과 사료 요구율에서 나타났다(P<0.05). 혈중 알부민, 총콜레스레롤 함량은 ME 3,100 kcal/kg 처리구에서 ME 3,000 kcal/kg 처리구보다 증가하였지만 중성지방은 감소되었다(P<0.05). 한편 총콜레스테롤은 CP (22)21 처리구에서 CP (22)20, (20)18% 처리구에 비하여 증가하였다(P<0.05). 시험 2 : $0{\sim}2$주간 증체량은 ME 3,000 kcal/kg 처리구에 비하여 ME 3,100 kcal/kg 처리구에서 높게 나타났으며, CP 22% 처리구에서 CP 20% 처리구보다 유의하게 개선되었으며, CP과 ME는 상호 작용을 보였다(P<0.05). 사료 요구율은 CP 21, 22% 처리구에서 CP 20% 처리구보다 개선되었으나(P<0.05), $3{\sim}5$주 및 전체 사육 기간에 처리구간에 생산성은 차이가 없었다. 혈액 성상도 처리구간에 차이를 나타내지 않지만 ME와 CP 처리구간 총콜레스테롤 함량에 대한 상호 작용은 <.0001 수준에서 인정되었다. 따라서 교잡육용계 생리에 적합한 사료내 단백질 수준은 $0{\sim}2$주령에 22% 또는 그 이상의 수준으로 급여하고, $3{\sim}5$주의 단백질 수준은 21, 20% 수준으로 급여가 생산성의 극대화에 효과적일 것으로 생각되며, 사료내 에너지는 부분적으로 처리구간에 생산에 차이를 보였지만 일관성이 없었으므로 에너지 수준을 세분화하여 급여하는 추후의 시험이 필요할 것으로 사료된다.

Methionine의 첨가수준이 돈육의 품질에 미치는 영향 (Effects of Methionine Addition Level on Quality of Pork Carcasses)

  • 이순자
    • 한국식품영양학회지
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    • 제4권1호
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    • pp.69-74
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    • 1991
  • This study was carried out to investigated the influence of methionine addition on quality of pork carcasses, 48 crossbred pigs were alloted to 4 treatments, NRC methionine requirement(A), additions of 0.035%(B), 0.07%(C) and 0.105%(D) to NRC methionine requirement. The results obtained are summarized as follows : 1. Cut meats were not significantly affected by methionine levels. But the treatment C showed slightly higher than the other treatments. 2. Chemical compositions of cut meats were not different from treatments. However, the treatment D showed slightly higher moisture, crude fat ands ash contents than other treatments. And crude protein content was better in treatment C than the other treatments. 3. There was no significant difference the among treatments, the best value of water bolding capacity was recorded with the treatment C, and pH value of the treatment A and D Increased as compared with the treatment B and C.

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Milk Protein Production and Plasma 3-Methylhistidine Concentration in Lactating Holstein Cows Exposed to High Ambient Temperatures

  • Kamiya, Mitsuru;Kamiya, Yuko;Tanaka, Masahito;Shioya, Shigeru
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권8호
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    • pp.1159-1163
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    • 2006
  • This experiment was performed to examine the influences of high ambient temperature on milk production, nutrient digestibility, energy and protein sufficiency ratio, and plasma metabolites concentration in lactating cows. In a $2{\times}2$ crossover design, four multiparous lactating Holstein cows were maintained in a chamber under treatment of constant moderate ($18^{\circ}C$) ambient temperature (MT) or high ($28^{\circ}C$) ambient temperatures (HT). The DMI and milk protein yield were significantly lower in HT (p<0.05). The milk yield, milk lactose yield, and milk SNF yield tended to be lower in HT (p<0.10). No statistical differences for 4% fat-corrected milk and milk fat yield were observed. Rectal temperatures were significantly higher in HT than MT (p<0.05). The apparent DM, OM, ether extract, CF, and ash digestibility did not differ between treatments. On the other hand, the apparent CP digestibility was increased significantly (p<0.05) and nitrogen free extract tended to increase (p<0.10) in HT. The sufficiency ratio of ME and DCP intake for each requirement tended to be lower in HT than in MT (p<0.10). Concentrations of total protein (TP), albumin, and urea nitrogen in plasma did not differ between treatments. Plasma 3-methylhistidine (3MH) concentration as a marker of myofibrillar protein degradation tended to be higher in HT (p<0.15). In conclusion, high ambient temperature was associated with a lower energy and protein sufficiency ratio, and decreased milk protein production, even though the body protein mobilization tended to be higher.