• 제목/요약/키워드: Protein Yield

검색결과 2,170건 처리시간 0.029초

산과 알칼리 pH에서 어육 단백질의 용해를 이용한 수리미 제조 (Surimi Processing Using Acid and Alkali Solubilization of Fish Muscle Protein)

  • 박주동;정춘희;김진수;조득문;조민성;최영준
    • 한국식품영양과학회지
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    • 제32권3호
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    • pp.400-405
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    • 2003
  • 산과 알칼리 수리미 제조공정을 확립하기 위하여 단백질 가용화와 회수를 위한 최적 pH, 이온강도의 영향, 균질화 조건, 마쇄육에 대한 수도수의 비 및 수율을 검토하고, 산 및 알칼리 수리미 공정에 따른 폐수의 오염 저감 효과를 수세수리미와 비교하였다. 어육 단백질을 추출하기 위한 최적 pH는 2.5와 10.5부근이었으며, 가용성 단백질의 침전 회수를 위한 최적 pH는 5.0 부근이었다. 추출 용액의 이온강도 증가는 어육 단백질의 추출량을 현저히 감소시키는 것으로 나타났고, 균질화를 위한 최적 조건은 9500 rpm 이하에서 30초였다. 원심분리 부하량과 파괴강도 및 변형값을 고려한 최적수량은 어육에 대하여 6배량이었다. 산 혹은 알칼리 공정의 수리미 수율은 수세 공정에 비하여 높았으며, 폐수의 오염 부하량은 현저히 낮은 것으로 나타났다.

유채박 단백질의 추출 및 정제에 관한 연구 (Extraction and Purification of Rapeseed Protein)

  • 이장순;강동섭;강영주
    • 한국식품과학회지
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    • 제22권7호
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    • pp.780-785
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    • 1990
  • 유채박 단백질의 효과적인 추출 및 정제공정을 확립하기 위하여 추출용매를 비교했으며, 1% SHMP 용매로 추출한 후 등전침전, 산세척, UF농축 처리에 의하여 정제한 단백질의 수율 및 glucosinolate와 phytate 잔존량을 조사하였다. 추출조건은 1% SHMP(pH 8.0) 용매가 가장 적당하였으며, glucosinolate 및 phytate 제거를 위한 정제효과 면에서는 2회 산세척시와 1회 산세척 및 UF(100K) 농축 처리한 공정이 가장 효과적 이었다. 수율과 단백질 함량은 2회 산세척의 경우는 37.1% 및 75.3%이었으며, 1회 산세척과 UF(100K) 농축의 경우는 42.1% 및 72.4%였다. 따라서 glucosinolate와 phytate 제거효과와 단백질의 수율면을 고려한 결과 가장 효과적인 정제공정은 등전침전${\rightarrow}$1회 산세척(pH 3.5)${\rightarrow}$중화(pH 7.5)${\rightarrow}$ UF(100K) 농축 처리하는 방법이다.

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The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment

  • Law, Fang Lin;Zulkifli, Idrus;Soleimani, Abdoreza Farjam;Liang, Juan Boo;Awad, Elmutaz Atta
    • Asian-Australasian Journal of Animal Sciences
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    • 제31권8호
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    • pp.1291-1300
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    • 2018
  • Objective: This experiment was conducted to investigate the effects of dietary crude protein (CP) level and exogenous protease supplementation on growth performance, serum metabolites, carcass traits, small intestinal morphology and endogenous protease activity in broiler chickens reared under a tropical climate. Methods: A total of 480 day-old male broiler chicks were randomly assigned to eight dietary treatments in a $4{\times}2$ factorial arrangement. The main effects were CP level (21.0%, 19.7%, 18.5%, or 17.2% from 1 to 21 days and 19.0%, 17.9%, 16.7%, or 15.6% from 22 to 35 days) and protease enzyme supplementation (0 ppm or 500 ppm). All experimental diets were fortified with synthetic feed-grade lysine, methionine, threonine and tryptophan to provide the minimum amino acid recommended levels for Cobb 500. Results: Reducing dietary CP linearly reduced (p<0.05) growth performance, serum albumin, total protein, and carcass traits and increased (p<0.05) serum triglycerides and abdominal fat. There was no consistent effect of reducing dietary CP on morphological parameters of the intestine and on the pancreatic and intestinal endogenous protease activity (p>0.05). Protease supplementation improved (p<0.05) feed conversion ratio, body weight gain, carcass yield and intestinal absorptive surface area. Conclusion: Protease supplementation, as measured by growth performance, intestinal morphology and carcass yield, may alleviate the detrimental effects of low protein diets in broiler chickens.

활성화된 Sepharose Gels에 공유결합으로 고정화된 Urokinase를 이용한 융합단백질 절단반응 (Fusion Protein Cleavage by Urokinase Covalentley Immobilized to Activated Sepharose Gels)

  • 서창우;강관엽;이효실;안상점;이은규
    • KSBB Journal
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    • 제15권1호
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    • pp.42-48
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    • 2000
  • 본 연구에서는 고정화 UK를 이용한 융합단백질의 절단방응에 대해 UK의 고정화, 고정화 UK의 특성과 절단방응, 절단반응 후의 분리정제 그리고 고정화 UK으 재생에 대해 실험하였다. 고정화 수율은 99% 이상이였고 고정화 후의 효소활성은 80%를 유지하였다. 융합단백질 전단반응에서 액상 UK와 고정화 UK를 이용한 회분식 반응 모두 약 70%의 절단반응을 얻었고, 특히 고정화 UK의 사용시 부반응이 매우 낮은 이점이 있었다. 컬럼식 절단반응에서는 기절의 주입속도에 따라 절단수율은 크게 변화하였다. 최적의 유속은 50%의 절단수율을 얻은 1 bed volume/h로 설정하였다. 고정화 효소반응의 이점인 안정성과 반복사용 측면에서는 액상 UK 대비 고정화 UK가 높은 열안정성을 보였고 낮은 pH에서는 10% 이상 높은 활성을 유지하였다. 반복사용을 위해 6M GuHCl을 사용하여 인위적으로 풀림, 재접힘을 한 경우 98%의 활성을 얻음으로 타당성이 있음을 제시하였다. 또한 목적 단백질의 분리를 위하여 산침전 후 expanded bed adsorption 크로마토그래피를 이용함으로써 연속화된 고수율의 정제공정을 가능하게 하였다. 이러한 고정화 UK를 이용한 절단방응 및 정제시스템을 구축함으로써 융합단백지의 생산공정에 매우 유용하게 사용될 것으로 생각되어진다.

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Composite genotypes of progestogen-associated endometrial protein gene and their association with composition and quality of dairy cattle milk

  • Kolenda, Magdalena;Sitkowska, Beata;Kamola, Dariusz;Lambert, Barry D.
    • Animal Bioscience
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    • 제34권8호
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    • pp.1283-1289
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    • 2021
  • Objective: The progestogen-associated endometrial protein (PAEP) gene encodes the main whey protein in milk, β-lactoglobulin. The aim of the study was to investigate polymorphism in the PAEP gene and its association with milk yield, composition, and quality. Methods: Test-day records for 782 dairy cows were analysed. A total of 10 single nucleotide polymorphisms (SNP) within the PAEP gene were investigated. The following parameters were recorded: milk yield (MY, kg/d), percent milk fat (%), protein (PP, %), dry matter (DMP, %) and lactose (LP, %), urea content (UC, mg/L) as well as natural logarithm for somatic cell count (LnSCC, ln). Effect on genomic estimated breeding values accuracy was evaluated with pedigree and single step model. Results: Results show that only three SNPs were polymorphic, creating 5 composite genotypes: P1 to P5. Differences in MY between composite genotypes were noted in the two tested herds. Cows with P5 composite genotypes were characterised by the highest PP and LnSCC and the lowest LP and UC (p<0.05). P4 was linked to an increased DMP and UC, while P3 to an increase in LP and decrease in PP and LnSCC. Both factors are important markers in herd management and have high influences on the herds economics. For 5 out of 7 traits the accuracy of prediction was improved by including the haplotype as a fixed effect. Conclusion: Presented results may suggest a new way to optimise breeding programmes and demonstrate the impact of using genomic data during that process.

Determination of Seeding and Harvesting Time in Snap Bean

  • Lee, Sang-Soon;Lee, Jeong-Dong;Hwang, Young-Hyun
    • 한국작물학회지
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    • 제46권1호
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    • pp.64-67
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    • 2001
  • Snap bean is a new corp in Korea but believed to have a great deal of potentials for both domestic and overseas markets. The present study was performed to obtain the basic information about growth- and quality-related characteristics and to determinate the optimum seeding date and harvesting time for snap bean. Pod yield was significantly affected by seeding date. The highest pod yield was obtained from March 20 for determinate type and April 4 for indeterminate one, respectively, with the range of 13.0-23.7 t/ha. The pod length of indeterminate type was over 13cm, and the pod length was over 5 grams. The pod width for tested varieties was less than 1.0cm. Considering the pod growth characters such as pod length, pod width, and pod weight, the optimum harvesting time for immature pods of snap bean was supposed to be from 15 to 20 days after flowering. The daily yield of snap bean was begun to sharply increase from 15 days after the first flowering and the maximum yield was recorded at 30 days after flowering. For the accumulated yield, nearly 90% of total yield was obtained in 42 days after flowering.

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초지경년이 건물수량 , 식생비율 및 사료가치에 미치는 영향 (effect of Pasture ages on the Dry Matter Yield , Botanical Composition and Forage Quality of Pasture Mixtures)

  • 한인규;김동암;조무환;이필상
    • 한국초지조사료학회지
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    • 제14권4호
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    • pp.264-270
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    • 1994
  • This experiment was undertaken to determine the effect of pasture ages on the total production and seasonal yield trends, botanical composition and forage quality of pasture mixtures. This experiment was conducted at the Livestock Experiment Station, RDA, Suweon. The results obtained were as follows : 1. Total dry matterDM) yield of pasture mixtures was the highest in the first year, and thereafter no significant reduction in the 2nd through the 4th year, but decreased by 30% in the 5th year. 2. The 34.4% of total DM yield was produced at the first cut, and decreased from then on. Total DM yield of pasture mixtures varied by year and climatic facton. In 1992, Total DM and TDN yields of pasture mixtures were 13.4 and 8.5 IvlT/ha, respectively. 3. The content of crude protein(CP), in virro DM digestibility(IVDMD) and TDN of pasture mixtures were 12-14, 70-80, and 62-65%, respectively. The highest forage quality was obtained at the 4th cutting. 4. The botanical compositions of legumes and weeds were increased as the year of establishment advanced, and highly increased at the 3rd cut after summer growth. 5. Based on the results of this study it appears that economic forage production could be expended to the 4th year with proper management, and the special renovation techniques may be needed for maintenance of pasture yield from the 5th year.

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바다새 (Diplachne fusca L.) 의 사료작물화 가능성에 관한 연구 II. 바다새의 생육특성, 사초수량 및 사료가치 (Studies on the Possible Utilization of Diplachne fusca L. as a Forage Crop II. Growth Characteristics, Forage Yield and Quality of Diplachne fusca L.)

  • 김창호;양주훈;이효원
    • 한국초지조사료학회지
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    • 제18권3호
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    • pp.179-186
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    • 1998
  • 이용율을 높이고 부존식물을 이용 조관료 생산 기반 구축에 필요한 기초 자료를 얻고자 바다새의 생육특성, 사초수량 및 사료가치를 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. 바다새의 생육은 출온기에 거의 완료되어 초장, 엽장, 엽폭, 경직경, 분얼수, 개체당 생체중 및 건물 중은 각각 137.5cm, 42.6cm, 4.65mm, 2.48mm, 15.3개, 4 44.6g 및 15.3g 이었다. 2. 생초수량은 출수기에 가장 많아 4,460kg/10a이었으며 건물수량은 출수기에 1,530kg/10a 이었던 것이 출수후에도 계속 증가하여 출수후 20일에는 1,630kg/10a이었다. 또한 5cm와 10cm의 예취높이 수준간 생초수량은 예취시기에 따라 유의성은 인정되었지만 총생초수량에서는 유의성이 없었다. 그러나 총건물수량에서는 유의성이 있어 10cm 예취높이구가 수량이 더 많았다. 3. 조단백질, 가급태단백질, 가소화단백질 및 TDN 함량은 생육이 진전됨에 따라 감소하여 각각 12.3~3.7%, 12.3~3.70, 10.8-3.6% 및 65.2~60.7% 범위였고, 조단백질, 가급태단백질 및 가소화단백질 수량은 출수기에 TDN 수량은 출수후 20일에 가장 높았다. ADF와 NDF 함량은 생육이 진전됨에 따라 증가하여 각각 출수전 20일에서 출수후 20일 사이에 3 36.4~50.0% 및 62.7~80.5%의 범위였다. 4. P, Ca, K 및 Mg 같은 무기물 함량은 생육이 진전됨에 따라 감소하여 각각 0.31~0.20%, 0.70~0.52%, 1.74~1.28% 및 0.19~0.18% 범위 이었으나 P, Ca 및 K의 수량은 출수기에 Mg 수량은 出수후 20일에 가장 많았다. 5. ENE, NEL, NEM 및 NEG 같은 정미에너지 함량도 생육이 진전됨에 따라 감소하여 각각 1.42~1.29, 0 0.68~0.62, 0.68~0.61 및 0.40~0.35Mcal/lb 범위 이었으며 이들 정미에너지 수량은 출수후에도 계속 증가하여 출수후 20일에 가장 높았다. 6. 바다새의 채식율은 출수전은 96.6% 출수후는 95.3%으로 나타나 대체로 기호성이 높았다.

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Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권12호
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).

Influence of flaxseed with rumen undegradable protein level on milk yield, milk fatty acids and blood metabolites in transition ewes

  • Ababakri, Rahmat;Dayani, Omid;Khezri, Amin;Naserian, Abbas-Ali
    • Journal of Animal Science and Technology
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    • 제63권3호
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    • pp.475-490
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    • 2021
  • An experiment was conducted to determine the effects of two levels of rumen undegradable protein (RUP) without or with whole or extruded flaxseed on milk yield, milk component, milk fatty acids (FAs) profile and plasma metabolites in transition ewes. Three weeks before and after lambing, seventy-two Baluchi ewes were used in a completely randomized design with a 3 × 2 factorial arrangement of treatments. The treatments contained 1) no flaxseed + 20% RUP (no flaxseed, low RUP [NFLR]); 2) no flaxseed + 40% RUP (no flaxseed, high RUP [NFHR]); 3) 10% whole flaxseed + 20% RUP (whole flaxseed, low RUP [WFLR]); 4) 10% whole flaxseed + 40% RUP (whole flaxseed, high RUP [WFHR]); 5) 10% extruded flaxseed + 20% RUP (extruded flaxseed, low RUP [EFLR]), and 6) 10% extruded flaxseed + 40% RUP (extruded flaxseed, high RUP [EFHR]). Ewes fed 10% extruded flaxseed exhibited higher (p < 0.001) dry matter intake (DMI) and colostrum yield (p < 0.1) compared to other treatments. Two types of flaxseed and RUP levels had no significant effect on milk yield, but milk fat and protein contents decreased and increased in diets containing 40% RUP, respectively. Ewes fed extruded flaxseed produced milk with lower concentrations of saturated fatty acids (SFA) and higher α-linolenic and linoleic acids and also polyunsaturated fatty acids (PUFA) compared to other groups (p < 0.05). During post-lambing, the ewes fed diets containing flaxseed exhibited higher concentration of serum non-esterified FAs (NEFA) compared to diets without flaxseed (p < 0.01). The concentration of serum β-hydroxybutyric acid (BHBA) decreased in the diets containing flaxseed types at pre-lambing, but increased in diets containing extruded flaxseed at post-lambing (p < 0.01). The serum glucose concentration of ewes (pre and post-lambing) which consumed diets containing extruded flaxseed or 40% RUP increased, but blood urea concentration was elevated following supplementation of diet with whole flaxseed or 40% RUP (p < 0.001). In conclusion, utilization of 10% extruded flaxseed in the diets of transition ewes had positive effects on animal performance with favorable changes in milk FAs profile. However, there is no considerable advantage to supply more than 20% RUP level in the diet of transition dairy sheep.