• Asian-Australasian Journal of Animal Sciences
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• 제12권5호
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• pp.728-734
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• 1999

Thirty new born crossbred (Bos taurus${\times}$Bos indicus) calves, divided randomly in a $3{\times}2$ factorial design, were fed calf starters containing one of three protein sources i.e., groundnut cake (GN), cottonseed meal (CS) and meat and bone meal (MB) along with either raw (M) or gelatinized maize (MG) for 90d. Milk was fed upto 56d of age. Green oats and respective calf starters were offered from 14d of age onwards ad lib. Clinical profile of serum suggested significantly (p<0.05) higher albumin and lower alanine aminotransferase activity due to CS feeding. Alklaine phosphatase activity varied significantly (p<0.05) among dietary treatments showing interaction between protein and starch sources. Inclusion of gelatinized maize resulted in significantly higher concentration of serum globulin (p<0.05) and alkaline phosphatase activity (p<0.01). reduced (p<0.05) ruminal pH was accompanied by a significant decrease (p<0.01) in $NH_3-N$ concentration in the strained rumen liquor (SRL) of MG fed calves. Ruminal amylase activity was lower (p<0.05) on MG diets. Alanine aminotransferase activity in the rumen exhibited a significant (p<0.01) interaction between protein and starch sources. While feeding of CS significantly (p<0.01) reduced alanine aminotransferase activity, inclusion of thermally processed maize reduced (p<0.01) both aspartate and alanine aminotransferase activities in the rumen. The overall blood picture was similar among treatments, whereas rumen metabolites especially enzyme activities, seems to be altered with source of degradable protein an starch.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.1040-1046
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• 2006

Studies were carried out with a repeated $4{\times}4$ Latin square design with eight cannulated pigs fed four experimental diets to investigate the effect of dietary fiber and starch sources on apparent ileal and fecal amino acid digestibility. Each period lasted 15 d, with diet acclimation from d 1 to 7, feces collection for 48 h on d 8 to 9 and ileal sample collection for 12 h on d 13 to 15. The four experimental diets consisted mainly of cooked rice with the addition of protein sources (CON), partial replacement of cooked rice with either potato starch (PS), sugar beet pulp (SBP) or wheat bran (WB). Chromic oxide was used as an indigestible marker. With the exception of histidine, lysine and tryptophan, no differences were observed in the apparent ileal digestibility of amino acids between diets. The inclusion of potato starch did not affect the ileal and fecal amino acid digestibility. In comparison with diet CON, a decreased (p<0.05) ileal digestibility of histidine was found in pigs fed diet SBP, while the ileal digestibilities of histidine, lysine and tryptophan were decreased (p<0.05) by the inclusion of wheat bran. Inclusion of fiber sources (sugar beet pulp and wheat bran) caused a reduction (p<0.05) in the fecal amino acid digestibility and the net disappearance of amino acids in the large intestine. Of the indispensable amino acids, there was a 'net synthesis' for methionine in the large intestine of pigs when diets were supplemented with dietary fiber. The decrease in fecal amino acid digestibility with the addition of dietary fiber indicates an increase in the synthesis of bacterial protein in the large intestine.

• Asian-Australasian Journal of Animal Sciences
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• 제13권9호
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• pp.1239-1244
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• 2000

In order to investigate the effect of incorporation of thermally processed cereal (maize) grain and differently degradable protein sources in the calf starter, twenty four newly born crossbred $(Bos\;taurus{\times}Bos\;indicus)$ calves were assigned at random to six diets in a $3{\times}2$ factorial design involving three protein sources viz. groundnut meal (GN), cottonseed meal (CS) and meat and bone meal (MB), each along with two differently processed grain, namely ground raw (R) and pressure cooked (P) maize. The corresponding calf starters with green oats (Avena sativa) were given free-choice from 14 d onwards till the end of the 90 d experimental feeding. A restricted milk diet was fed till the age of weaning at 60 d. Total DM intake was not affected by cereal or protein sources. However, daily intake of DM (59.23 vs 66.45 g) and CP (12.38 vs 14.10 g) per kg $W^{0.75}$ was reduced (p<0.05) due to cereal processing. Better (p<0.05) feed and protein efficiencies after weaning and during entire period in calves fed processed maize resulted in a trend of higher $(p{\leq}092)$ growth rate especially when GN was the source of protein. In comparison among protein sources, calves fed MB diets tended to grow faster $(p{\leq}098)$ concurrent with a higher CP intake before weaning. It is thus evident that thermal processing of maize in the calf starter seems to improve calf performance. Moreover, results indicated that feeding of protein and starch sources of matching ruminal degradability may prove beneficial for early growth of crossbred calves.

• 한국균학회지
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• 제15권4호
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• pp.231-237
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• 1987

1. Cassava의 전분을 이용하여 검정곰팡이(Aspergillus niger)를 $30^{\circ}C$에서 4일 동안 진탕배양 하였을 때 배지 중 Cassava의 전분의 함량이 4%, 6%, 8% 및 10%일 때 96시간 배양된 각각의 균체량은 13.35mg/ml, 15.78mg/ml, 14.48mg/ml 및 9.93mg/ml이었다. 2. Cassava의 전분을 이용하여 검정곰팡이를 $30^{\circ}C$에서 4일 동안 진탕배양하여 얻어진 균체를 Lowry 방법에 의하여 그 균체단백을 측정한 바 배지 중의 Cassava의 전분의 함량이 4%, 6%, 8% 및 10%일 때 각각의 균체단백은 2.6mg/ml, 2.7mg/ml, 2.5mg/ml 및 1.7mg/ml이었으며 6% 함유구에서 균체단백의 양이 가장 많았다. 3. 배지 중의 Cassava의 전분량을 6%로 맞추고 질소원의 종류를 $NaNO_3,\;(NH_4)_2SO_4$ 및 Urea를 사용하고 질소량을 Czapeck-Dox 배지에 있는 $NaNO_3$의 질소량의 50%, 100%, 200%, 400% 및 $800^{\circ}C$되게 변화시키면서 진탕배양한 바 균체생산량에는 차이가 없었다.

• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.15.1-15.5
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• 2016

Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, nitrogen-free extract, and energy in selected carbohydrate sources including wheat flour (WF), ${\alpha}-potato$ starch (PS), ${\alpha}-corn$ starch (CS), Na alginate (AL), dextrin (DEX), and carboxymethyl cellulose (CMC) were determined for olive flounder. The olive flounder averaging $150{\pm}8.0g$ were held in 300-L tanks at a density of 30 fish per tank. Chromic oxide was used as the inert marker. Feces were collected from the flounder by a fecal collector attached to a fish rearing tank. Apparent dry matter and energy digestibilities of flounder fed WF, PS, CS, and DEX diets were significantly higher than those of fish fed AL and CMC diets. Apparent crude protein digestibility coefficients of flounder fed PS and CS diets were significantly higher than those of fish fed AL, DEX, and CMC diets. Apparent crude lipid and nitrogen-free extract digestibility coefficients of flounder fed PS and DEX diets were significantly higher than those of fish fed WF, CS, AL, and CMC diets. The present findings indicate that PS and DEX could be effectively used as dietary carbohydrate energy compared to WF, CS, AL, and CMC for olive flounder.

• Asian-Australasian Journal of Animal Sciences
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• 제26권3호
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• pp.378-385
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• 2013

The aim of this study was to examine the effect of variety and growing conditions of wheat on broiler performance and nutrient digestibility. One hundred and sixty-four wheat samples, collected from a wide range of different sources, locations, varieties and years, were analyzed for a range of chemical and physical parameters. Chemical and physical parameters measured included specific weight, thousand grain weight (TG), in vitro viscosity, gross energy, N, NDF, starch, total and soluble non-starch polysaccharides (NSP), lysine, threonine, amylose, hardness, rate of starch digestion and protein profiles. Ninety-four of the wheat samples were selected for inclusion in four bird trials. Birds were housed in individual wire metabolizm cages from 7 to 28 d and offered water and feed ad libitum. Dry matter intake (DMI), live weight gain (LWG) and gain:feed were determined weekly. A balance collection was carried out from 14 to 21 d for determination of apparent metabolizable energy (AME), ME:gain, DM retention, oil and NDF digestibility. At 28 d the birds were sacrificed, the contents of the jejunum removed for determination of in vivo viscosity and the contents of the ileum removed for determination of ileal DM, starch and protein digestibility. The wheat samples used in the study had wide-ranging chemical and physical parameters, leading to bird DMI, LWG, gain:feed, ME:GE, AME content and ileal starch and protein digestibility being significantly (p<0.05) affected by wheat sample. A high level of N fertilizer application to the English and NI wheat samples tended to benefit bird performance, with increases of up to 3.4, 7.2 and 3.8% in DMI, LWG and gain:feed, respectively. Fungicide application also appeared to have a positive effect on bird performance, with fungicide treated (+F) wheat increasing bird DMI, LWG and gain:feed by 6.6, 9.3 and 2.7%, over the non-fungicide treated (-F) wheats. An increase (p<0.1) of 9.3% in gain:feed was also observed at the low seed rate of 40 compared to 640 seeds/$m^2$. It was concluded that the type of wheat sample and environmental growing conditions significantly affects bird performance when fed wheat-based diets.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1400-1410
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• 2004

Eight crossbred (75% Holstein Friesian) cows in mid-lactation were randomly assigned to a switchback design with a 2x2 factorial arrangement to evaluate two nonstructural carbohydrate (NSC) sources (corn meal and cassava chips) with different rumen degradability and used at two levels of NSC (55 vs. 75%) with protein source (supplied by urea in the concentrate mix). The treatments were 1) Low degradable low level of corn (55%) 2) Low degradable high level of corn (75%) 3) High degradable low level of cassava (55%) and 4) High degradable high level of cassava (75%). The cows were offered the treatment concentrate at a ratio to milk yield at 1:2. Urea-treated rice straw was offered ad libitum as the roughage and supplement with 1 kg/hd/d cassava hay. The results revealed that total DM intake, BW and digestion coefficients of DM were not affected by either level or source of energy. Rumen fermentation parameters; NH3-N, blood urea nitrogen and milk urea nitrogen were unaffected by source of energy, but were dramatically increased by level of NSC. Rumen microorganism populations were not affected (p>0.05) by source of energy, but fungal zoospores were greater for cassava-based concentrate than corn-based concentrate. Milk production and milk composition were not affected significantly by diets containing either source or level of NSC, however concentrate than corn-based concentrate averaging (4.4 and 4.2, respectively). Likewise, income over feed, as estimated from 3.5% FCM, was higher on cassava-based concentrate than corn-based concentrate averaging (54.0 and 51.4 US$/mo, respectively). These results indicate that feeding diets containing either cassava-based diets and/or a higher of oncentrates up to 75% of DM with NPN (supplied by urea up to 4.5% of DM) can be used in dairy rations without altering rumen ecology or animal performance compared with corn-based concentrate.

• Journal of Animal Science and Technology
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• 제45권3호
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• pp.421-432
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• 2003

옥수수 알곡의 무처리(통 옥수수 2종) 및 가공방법(분쇄, flake 3.8mm, 2.8mm 및 1.5mm)이 in sacco 방법에 있어 반추위 내에서의 전분 및 단백질 분해율에 미치는 영향을 측정하고자 반추위에 캐뉼라(cannulae)가 장착된 홀스타인종 건유우 3두를 공시축으로 하여, 본 시험을 수행하였는데 그 결과를 요약하면 다음과 같다. 1)48시간대에서의 전분 분해율은 1.5mm, 2.8mm flake, 분쇄 옥수수, 3.8mm flake, density 660g/$\ell$, density 740 g/$\ell$인 통 옥수수 순으로 높았다. Flake의 가공정도를 증진시키면, 즉 3.8mm에서 2.8mm로 얇게 가공하면 전분 분해율이 유의하게 증가되었으며, 1.5mm로 더욱 얇게 가공하면 2.8mm 보다 증가하는 경향을 보였으나 유의적인 차이는 없었다(P<0.05). 12시간대에서 24시간대까지는 density가 낮은 통 옥수수가 높은 것보다 많이 분해되는 경향을 보였으나, 유의적인 차이가 없었고, 48시간대에서는 유의하게 높았다(P<0.05). 2) 단백질 분해율은 반추위내 배양시간이 경과함에 따라 모두 증가하는 경향이었다. 48시간대에서의 단백질 분해율은 분쇄 옥수수, 1.5 mm, 2.8mm, 3.8mm flake의 순으로 높았다(P<0.05). Flaking 처리시의 단백질 분해율은 분쇄 옥수수보다 유의하게 낮아졌으나, 8${\sim}$48시간대에서는 2.8mm, 1.5mm로 가공정도를 증진시킬수록 높아지는 경향이었다. 3) “a”값, 즉 물에 녹는 수용성 부분을 계산한 결과, 단백질의 “a”값은 flake가 분쇄 옥수수보다 유의하게 낮았다. 반면에 전분의 “a”값은 flake가 분쇄 옥수수보다 높았다. 곡류를 flaking 처리하면 전분이 젤라틴화 되어, 반추위 내에서 전분 분해속도는 증가되고, 단백질 분해속도는 감소되기 때문이라고 생각된다. 이와 같이 사료원료의 물리적 특성은 반추위내 배양시간별 영양소 이용율에 양향을 미친다. 그러므로, 반추가축 사료에 이용되는 원료들의 이와 같은 분해특성을 적절히 고려하면 단백질원과 탄수화물원의 반추위내 발효 동기화를 보다 최적화할 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권2호
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• pp.263-270
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• 2004

A repeated $4{\times}4$ Latin square design was conducted with eight ileal cannulated castrates to examine the effect of source of starch and fiber on nutrient balance and energy metabolism. Pigs were fed on one of the four experimental diets: Control diet (C) mainly based on cooked rice; and diets P, S and W with the inclusion of either raw potato starch, sugar beet pulp or wheat bran supplementation, respectively. With the exception of an increased (p<0.05) energy loss from methane production with diet S observed, no significant differences (p>0.05) in the ratio of metabolizable energy (ME)/digestible energy, the utilization of ME for fat deposition and for protein deposition, energy loss as hydrogen and urinary energy were found between diets. The efficiency of utilization of ME for maintenance was lower (p<0.05) with diets P and S than with diet C. The inclusion of fiber sources (sugar beet pulp or wheat bran) or potato starch reduced the maintenance energy requirement. The fecal energy excretion was increased (p<0.05) with either sugar beet pulp or wheat bran supplementation, while it was unaffected (p>0.05) by addition of potato starch. In comparison with diets C and P, a lowered ileal or fecal digestibility of energy with diets S and W was observed (p<0.05). Feeding sugar beet pulp caused increased (p<0.05) daily production of methane and carbon dioxide and consequently increased energy losses from methane and carbon dioxide production, while it did not influence the daily hydrogen production (p>0.05). An increased (p<0.05) proportion of NSP excreted in feces was seen by the supplementation of wheat bran. Higher NSP intake caused an increased daily amount of NSP in the ileum, but the ileal NSP proportion as a percentage of NSP intake was unaffected by diets. Feeding potato starch resulted in increased daily amount of starch measured in the ileum and the proportion of ileal starch as a percentage of starch intake, while no significant influence on fecal starch was found. Higher (p<0.05) daily amount of fecal starch and the proportion of fecal starch as a percentage of starch intake were found with fiber sources supplementation compared with diets C and P. By increasing the dietary NSP content the fecal amount of starch increased (p<0.01).

• Animal Bioscience
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• 제36권10호
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• pp.1568-1577
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• 2023

Objective: A study was conducted to determine the chemical composition of banana meal and rice bran from Australia or South-East Asia and test the hypothesis that there are no differences in rice bran produced in different countries, but there are differences between full-fat and defatted rice bran. Methods: Two sources of banana meal and 22 sources of rice bran (full-fat or defatted) from Australia or South-East Asia were used. All samples were analyzed for dry matter, gross energy, nitrogen, amino acids (AA), acid hydrolyzed ether extract (AEE), ash, minerals, total starch, insoluble dietary fiber, and soluble dietary fiber. Banana meal was also analyzed for sugars including glucose, fructose, maltose, sucrose, stachyose, and raffinose. Results: Chemical analysis demonstrated that banana meal from the Philippines is primarily composed of starch. Full-fat rice bran from Australia had greater (p<0.05) concentrations of AEE, lysine, and glycine than samples from the Philippines and Vietnam. Full-fat rice bran from Australia and Thailand had greater (p<0.05) concentrations of gross energy and most AA than rice bran from Vietnam. Full-fat rice bran from Australia had greater (p<0.05) concentrations of tryptophan and manganese than all other sources, but full-fat rice bran from the Philippines contained less (p<0.05) zinc than all other sources of rice bran. Gross energy, AEE, and copper were greater (p<0.05) in full-fat rice bran compared with defatted rice bran, but defatted rice bran contained more (p<0.05) crude protein, ash, insoluble dietary fiber, total dietary fiber, AA, and some minerals than full-fat rice bran. Conclusion: Banana meal is a high-energy source that can be used as an alternative ingredient in livestock diets. Full-fat rice bran from Australia and Thailand contained more concentrations of AEE and AA than samples from the Philippines or Vietnam. Full-fat rice bran had more gross energy and AEE than defatted rice bran, whereas defatted rice bran contained more crude protein, ash, and total dietary fiber.