• Title/Summary/Keyword: Protein Retention

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The Effect of Soybean Galactooligosaccharides on Nutrient and Energy Digestibility and Digesta Transit Time in Weanling Piglets

  • Zhang, Liying;Li, Defa;Qiao, Shiyan;Wang, Jituan;Bai, Lu;Wang, Zongyi;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.11
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    • pp.1598-1604
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    • 2001
  • Eight $12.4{\pm}0.6kg$ initial body weight crossbred barrows were used to determine the effect of soybean galactooligosaccharides on nutrient and energy digestibility, and digesta transit time. Four dietary treatments were utilized in this trial. Treatment one was a corn-soybean meal based diet (SBM) containing raffinose and stachyose at the levels of 0.16% and 0.75%, respectively. Treatment two (control) was a corn-HP300 (soybean concentrate protein) diet. In treatments three and four, 1.1% and 2.2% commercial stachyose was added to the control diet to provide total dietary stachyose at the levels of 1% and 2%, respectively. The soybean galactooligosaccharides (raffinose + stachyose) level in treatment one was slightly lower compared to that in treatment three. Three collection periods were run with two pigs for each treatment/period. There was a 4 d adjustment period followed by a 3 d collection period. The results showed that the nitrogen retention (86.79%) of pigs fed treatment two diet was higher than that of pigs fed treatment one by 5.2% (p<0.05). The nitrogen retention of treatment three was intermediate 83.09%. The apparent fecal digestibility of all amino acids in treatment two was numerically highest, followed by treatments three and four. However, there were no significant difference among groups (p>0.05). The dry matter (DM), organic matter (OM), crude protein (CP), and crude fiber (CF) digestibility numerically decreased as the soybean galactooligosaccharides level increased, but were not significantly different (p>0.05). Chromium content in feces (from the inclusion of 0.3% chromic oxide in the diets) differed among treatments (p<0.05) at 15 h, 18 h, and 21 h after eating. This showed that the digesta transit time was differed significantly among treatments. Treatment four was the shortest, followed by treatment three, SBM and control. The results demonstrated that in the absence of antinutritional factors and soybean antigen protein, inclusion of 1% and 2% stachyose in corn-HP300 diet has no significant effect on the digestibility of DM, OM, CP, CF and amino acids. When the soybean galactooligosaccharide level in diet one and diet three were adjusted to be almost the same, antinutritional factors such as trypsin inhibitor and soybean antigen protein could decrease the nutrient digestibility and nitrogen retention rate of diet. High levels of soybean galactooligosaccharides shortened the digesta transit time in the intestinal tract. This trial suggested that the total level of soybean galactooligosaccharides (stachyose+raffinose) in the weanling piglet diet is better not to exceed 1% when common soybean meal is used as main protein source.

Effect of Dietary Protein and Cysteine Levels on Cadmium Toxicity in Rats (식이 단백질과 cysteine 수준이 흰쥐의 Cadmium 중독에 미치는 영향)

  • 김미경
    • Journal of Nutrition and Health
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    • v.29 no.5
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    • pp.461-471
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    • 1996
  • This study was performed to investigate the effect of dietary protein and cysteine levels on cadmium toxicity in rats. Seventy-two male rats of Sprague-Dawley strain weighting 171$\pm$3g were blocked into 12 groups according to body weight, and were raised for 30 days. cadmium chloride was given at levels of 0 or 400ppm, protein at levels of 7, 15 and 40%, and cysteine was added(total dietary cysteine contents : 0.45%) to diet or not. The results are summarized as follow. Food intake, weight gain, food were lower than those of cadmium free group. But, these were increased with increasing dietary protein level and cysteine addition. Fecal cadmium excretion was remarkably increased in high protein (40%) groups. Thus, cadmium retention rates were decreased in high protein groups. Metallothionein concentrations in liver and kidney were increased in cysteine addition, and cadmium administration. Especially, these were remarkably increased in cadmium and cysteine added groups. Urinary calcium excretion was increased with cadmium administration, but urinary protein excretion and creatinine clearance were not changed in these animal. In conclusion, food intake, weight gain and organ weights were decreased with administration. Cadmium toxicity was alleviated by increasing fecal cadmium excretion, while cysteine addition increased metallothionein concentrations in liver and kidney. From these results, it was shown that cadmium toxicity was alliviated by synergistic effect of high protein level and cysteine addition.

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Effect of Close-up Dry Period Protein Level on Preparturiental Nitrogen Balance and Lactating Performance of Primigravid and Multiparous Holstein Cows

  • Adachi, N.;Kusuhara, T.;Nonaka, I.;Terada, F.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.6
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    • pp.831-836
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    • 2006
  • This study compared the effects of two levels of crude protein (CP) supplementation during a close-up dry period on the productive performance, nitrogen balance, and blood profiles of Holstein cows. Eighteen cows (8 primigravid and 10 multiparous), 21 days prior to calving, were divided into four groups and fed a diet containing a low (11.5 to 12.7% CP) or high (14.1 to 15.3% CP) level of protein. Following parturition, all cows were offered the same diet. Nitrogen balance was measured 12 d to 10 d prior to the expected calving date. Whole feces and urine were collected. The higher close-up CP did not improve milk production during the early lactation period (14 w) in multiparous cows but tended to increase production by 2 kg of milk per day in primigravid cows. There were no differences in the weight of calves, the weight of the placenta, or the incidence of calving difficulty. Nitrogen intake, urine nitrogen and nitrogen retention were increased by the higher CP diet in primigravid and multiparous cows. All groups of cows showed a positive nitrogen balance. In particular, higher nitrogen retention was observed in primigravid cows fed the higher CP diet. With the exception of plasma urea nitrogen (PUN), non of the blood profiles were influenced by the CP level. The higher close-up CP increased the PUN during the period studied in primigravid and multiparous cows. These results indicate that a CP level of 14% during a close-up dry period is optimal for primigravid cows, and 12% CP level is sufficient for multiparous cows.

Effect of Solvent in the Dyeing System of Acid Dye/Human Hair (산성염료/모발 염색계에 있어서 용매의 효과)

  • Lee, Jin;Lim, Sun-Nye;Jung, Nam-Young;Lee, Woong-Eui;Choi, Chang-Nam
    • Textile Coloration and Finishing
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    • v.23 no.4
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    • pp.250-256
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    • 2011
  • In this work, the effects of surfactant and solvents, such as benzyl alcohol and n-propyl alcohol, on the human hair dyeing with acid dye was investigated including the amount of dyes in hair according to dyeing time, temperature, and pH. And the damage of dyed hair was assessed by water retention, protein release, and SEM images of the dyed hairs. By adding benzyl alcohol in acid dyeing, the dyeing rate was increased and the dyeing equilibrium was established at early stage. The water retention of the dyed hair was increased and the protein release of the dyed hair was decreased. Therefore the hair was damaged less during the dyeing.

Effect of Solvent in Human Hair Dyeing with Natural Dye(II) (천연염료에 의한 모발염색에서 용매의 효과(II))

  • Choi, Chang-Nam;Yang, Hye-Youn;Jung, Nam-Young;Lim, Sun-Nye;Lee, Woong-Eui;Jang, Mi-Hwa
    • Textile Coloration and Finishing
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    • v.22 no.2
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    • pp.110-117
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    • 2010
  • Recently, there is a trend to utilize natural dyes in many dyeing fields. In this work, the effects of benzyl alcohol in human hair dyeing with cochineal, a natural dye, was investigated. We investigated the K/S value and color value of dyed hair, water retention of dyed hair, protein release-ability of dyed hair, and wash fastness of dyed hair according to dyeing time and temperature. The shade of dyed hair was reddish. By adding benzyl alcohol in cochineal dyeing, the dyeing rate was increased and the dyeing equilibrium was established at early stage. The water retention of dyed hair was increased and the protein release-ability of dyed hair was decreased, meaning that the hair was less damaged during dyeing, The dyed hair showed a good wash fastness.

Growth, Feed Utilization and Nutrient Retention of Juvenile Olive Flounder (Paralichthys olivaceus) Fed Moist, Semi-moist and Extruded Diets

  • Kim, J.-D.;Shin, S.-H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.5
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    • pp.720-726
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    • 2006
  • In an attempt to develop an artificial diet for growing olive flounder (Paralichthys olivaceus), weight gain, feed utilization and nutrient retention were investigated in fish fed moist (MP), semi-moist (SMP) and extruded pellets (EP). Excretion of nitrogen and phosphorus was also estimated based on their whole body gain and intake. EP and MP composed of raw fish and SMP made of formulated powder feed with water were prepared to have the same energy contents on a dry matter basis. A total of 240 fish with an average initial weight of 120 g were randomly distributed to each (20 fish/tank) of 12 circular plastic tanks (4 tanks/treatment) and fed experimental diets for 8 weeks. Fish groups fed EP (209 g) or SMP (209 g) recorded higher final weight than those fed MP (176 g), while dry feed consumption was highest in SMP groups (106 g), followed by MP (71 g) and EP groups (62 g). As a consequence, fish groups fed EP showed much lower feed conversion ratio than the other two groups. Protein efficiency ratio was also significantly higher in fish groups fed EP (2.55) than in those fed MP (1.44) and SMP (1.31). Fish groups fed EP, which showed the highest nitrogen retention of 43.9%, resulted in the lowest excretion of nitrogen of 35.5 g per kg gain. Also, the lowest phosphorus excretion of 6.0 g per kg gain was found in the EP groups with the highest P retention (37.0%) among treatments. Although the EP groups had the lowest dietary energy intake, they retained the highest energy in the whole body among treatments. The present results showed that EP could be more advantageous than MP or SMP in terms of growth, feed utilization and excretion of nitrogen and phosphorus for olive flounder.

Effect of Dietary Cadmium Levels on Nutrient Digestibility and Retention of Iron, Copper and Zinc in Tissues of Growing Pigs

  • Han, X.Y.;Xu, Z.R.;Wang, Y.Z.;Tao , X.;Li, W.F.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.7
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    • pp.1007-1013
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    • 2004
  • This experiment was conducted to investigate the effect of cadmium levels on weight gain, nutrient digestibility and the retention of iron, copper and zinc in tissues of growing pigs. A total of one hundred and ninety-two crossbred pigs (barrows, Duroc$\times$Landrace$\times$Yorkshine, 27.67$\pm$1.33 kg of average initial body weight) were randomly allotted to four treatments. Each treatment had three replicates with 16 pigs per pen. The corn-soybean basal diets were supplemented with 0, 0.5, 5.0, 10.0 mg/kg cadmium respectively, and the feeding experiment lasted for eight-three days. Cadmium chloride was used as cadmium source. The results showed that pigs fed the diet containing 10.0 mg/kg cadmium had lower ADG and FCR than any other treatments (p<0.05). Apparent digestibility of protein in 10.0 mg/kg cadmium-treated group was lower than that of other groups (p<0.05). There was lower iron retention in some tissues of 5.0 mg/kg and 10.0 mg/kg cadmium treatments (p<0.05). However, pigs fed the diet 10.0 mg/kg cadmium had higher copper content in most tissues than that of any other groups (p<0.05). There was a significantly increase of zinc retention in kidney of 10.0 mg/kg cadmium additional group (p<0.05) and zinc concentrations in lymphaden, pancreas and heart of 10.0 mg/kg cadmium treatment were lower than those of the control (p<0.05). This study indicated that relatively high cadmium level (10.0 mg/kg) could decrease pig growth performance and change the retention of iron, copper and zinc in most tissues during extended cadmium exposure period.

Comparison of Nutrient Content and Retention Rate among Chuichung Cucumber, White Dadagi Cucumber, and Yellowish Overripe Cucumber according to Different Cooking Methods (취청오이, 백다다기오이, 노각의 조리방법에 따른 영양 성분 및 잔존율 연구)

  • Kim, Honggyun;Chung, Heajung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.11
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    • pp.1350-1357
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    • 2017
  • General component and mineral contents according to species of Capsicum annuum were investigated. In addition, chuichung cucumber, white dadagi cucumber, and yellowish overripe cucumber were cooked by using seven different methods (boiling, steaming, pan-cooking, roasting, pan-frying, deep-frying, and microwaving), and retention rates of general components and minerals according to species were evaluated. Weight loss was observed in all cucumber species using all cooking methods. Most general component and mineral contents of C. annuum samples were significantly different according to species, and cooking method (P<0.05), whereas Fe and Cu contents were not significantly affected by species and cooking method, respectively. As a result, the highest protein, fat, ash contents were detected in deep-fried C. annuum, and high moisture contents were detected in raw, boiled, and steamed C. annuum. High mineral contents were detected in roasted and microwaved chuichung cucumber, deep-fried white dadagi cucumber and yellowish overripe cucumber. Retention rates of C. annuum by cooking method, ranged from 30~100% for protein, 10~100% for fat, 35~90% for moisture, and 30~100% for ash. High retention rates were observed for minerals in boiled, roasted, and microwaved chuichung cucumber, and deep-fried white dadagi cucumber and yellowish overripe cucumber. However, in yellowish overripe cucumber, mineral retentions rate were remarkably lowered by boiling and steaming, resulting in rates of 20~45%. These results were the data used as the basis for development of cucumbers.

Increasing the Flow of Protein from Ruminal Fermentation - Review -

  • Wallace, R.J.;Newbold, C.J.;Bequette, B.J.;MacRae, J.C.;Lobley, G.E.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.6
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    • pp.885-893
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    • 2001
  • This review summarizes some recent research into ways of improving the productivity of ruminal fermentation by increasing protein flow from the rumen and decreasing the breakdown of protein that results from the action of ruminal microorganisms. Proteinases derived from the plant seem to be of importance to the overall process of proteolysis in grazing animals. Thus, altering the expression of proteinases in grasses may be a way of improving their nutritive value for ruminants. Inhibiting rumen microbial activity in ammonia formation remains an important objective: new ways of inhibiting peptide and amino acid breakdown are described. Rumen protozoa cause much of the bacterial protein turnover which occurs in the rumen. The major impact of defaunation on N recycling in the sheep rumen is described. Alternatively, if the efficiency of microbial protein synthesis can be increased by judicious addition of certain individual amino acids, protein flow from ruminal fermentation may be increased. Proline may be a key amino acid for non-cellulolytic bacteria, while phenylalanine is important for cellulolytic species. Inhibiting rumen wall tissue breakdown appears to be an important mechanism by which the antibiotic, flavomycin, improves N retention in ruminants. A role for Fusobacterium necrophorum seems likely, and alternative methods for its regulation are required, since growth-promoting antibiotics will soon be banned in many countries.

Optimal Dietary Protein and Lipid Levels for Growth of Long-nosed Barbel, Hemibarbus longirostris

  • Kim, Yi-Oh;Hwang, Gyu-Deok;Lee, Sang-Min
    • Fisheries and Aquatic Sciences
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    • v.12 no.4
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    • pp.311-316
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    • 2009
  • A 10-week feeding trial with four dietary protein levels (22%, 32%, 42% and 52%) and two dietary lipid levels (8% and 17%) was conducted to investigate the optimum dietary protein and lipid levels for growth of long-nosed barbel fingerlings. Survival rate of fish was not affected by either the dietary protein or the dietary lipid level. Weight gain and feed efficiency were affected by the dietary protein level (P<0.01), but not by the lipid level, and increased with the dietary protein level at the both lipid levels. Weight gain and feed efficiency of fish fed the 52% protein diets with 8-17% dietary lipids were not significantly different from those of fish fed the 42% protein diets with 8-17% dietary lipids and 32% protein diet with 17% dietary lipid. Daily feed intake of fish was not affected by either dietary protein or dietary lipid level. Protein efficiency ratio and protein retention rate of fish fed the 32% protein diet with 17% dietary lipid were significantly higher than those of fish fed the 52% protein diets with 8-17% dietary lipids. Moisture content of fish fed the diets containing 8% lipid were higher than those of fish fed the diets containing 17% dietary lipid at each protein level. Crude lipid content of fish fed the diets containing 17% dietary lipid were higher than that of fish the fed the diet containing 8% dietary lipid at each protein level. The results of this study indicated that 32% protein and 17% lipid could be the optimum dietary level for growth of juvenile long-nosed barbel.