• 한국가금학회지
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• 제30권2호
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• pp.73-81
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• 2003

본 연구는 산란종계 육성 사료 내의 에너지 및 단백질 수준이 육성기 성장에 미치는 영향을 규명하고자 실시하였다 .8일령의 Hy-Line Variety Brown 갈색 산란종계 암평아리 360수를 5개 처리에 처리당 4반복, 반복당 18수씩 완전 임의 배치하여 에너지와 단백질 수준이 다른 실험 사료를 9주간 급여하였다 .실험 기간 중 2주 간격으로 사료섭취량과 공시계의 생체중, 정강이 길이를 개체별로 측정하였고, 성장 균일도를 조사하였다. 실험 종료 시에 반복별로 평균체중에 해당하는 개체를 2수씩 희생시킨 후 우측 경골을 적출하여 경골 중량, 길이, 파쇄 강도 및 경골 내 회분 함량 등을 조사하였다 .실험종료 시, 증체량과 정강이 길이에서는 대조구에 비해 단백질 함량을 증가시킨 처리구에서 유의하게 증가하는 (P<0.05) 결과가 관찰되었다 사료섭취량에서는 처리간에 큰 차이가 없는 것으로 나타났다. 경골 길이 및 중량은 성장 성적의 증가와 상응해 증가하는 결과가 관찰되었고, 경골 강도 및 경골 내 회분 함량 역시 에너지 및 단백칠 수준의 증가에 따라 증가하는 결과 (P<0.05) 가 나타났다. 성장 균일도는 에너지 및 단백질 수준이 높은 처리구에서 실험 종료시인 10주째 증가하는 경향이 관찰되었으나, 처리간에 통계적 유의성 있는 차이는 인정되지 않았다. 본 연구에서 국내의 산란종계 병아리의 육성에서 NRC(1994) 사양표준의 권장 수준보다 대사에너지와 단백질 수준을 높인 육성 사료 또는 종계회사의 권장 수준에 가까운 에너지 및 단백질 수준의 육성 사료가 산란종계 병아리의 성장에 가장 적합한 영양 수준인 것으로 나타났다. 즉 Phase I 은 에너지 2800 ∼ 2950 kcal/kg, 단백질 19% 정도인 T3 구가 육성초기 가장 적합한 영양수준이고, Phase II에서도 T3, 즉 에너지 2800 ∼ 2950 kcal/kg, 단백질 16%수준이 가장 육성중기 병아리 발달에 가장 적합한 영양수준인 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제28권6호
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• pp.1022-1029
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• 2018

Tuberculosis (TB) remains a serious health issue around the word. Adenovirus (Ad)-based vaccine and modified vaccinia virus Ankara (MVA)-based vaccine have emerged as two of the most promising immunization candidates over the past few years. However, the performance of the homologous and heterologous prime-boost immunization regimens of these two viral vector-based vaccines remains unclear. In the present study, we constructed recombinant Ad and MVA expressing an Ag85B-TB10.4 fusion protein (AdH4 and MVAH4) and evaluated the impact of their different immunization regimens on the humoral and cellular immune responses. We found that the viral vector-based vaccines could generate significantly higher levels of antigen-specific antibodies, $IFN-{\gamma}$-producing splenocytes, $CD69^+CD8^+$ T cells, and $IFN-{\gamma}$ secretion when compared with bacillus Calmette-$Gu{\acute{e}}rin$ (BCG) in a mouse model. AdH4-containing immunization regimens (AdH4-AdH4, AdH4-MVAH4, and MVAH4-AdH4) induced significantly stronger antibody responses, much more $IFN-{\gamma}$-producing splenocytes and $CD69^+CD8^+$ T cells, and higher levels of $IFN-{\gamma}$ secretion when compared with the MVAH4-MVAH4 immunization regimen. The number of $IFN-{\gamma}$-producing splenocytes sensitive to $CD8^+$ T-cell restricted peptides of Ag85B (9-1p and 9-2p) and Th1-related cytokines ($IFN-{\gamma}$ and $TNF-{\alpha}$) in the AdH4-MVAH4 heterologous prime-boost regimen immunization group was significantly higher than that in the other viral vector-based vaccine- and BCG-immunized groups, respectively. These results indicate that an immunization regimen involving AdH4 may have a higher capacity to induce humoral and cellular immune responses against TB in mice than that by regimens containing BCG or MVAH4 alone, and the AdH4-MVAH4 prime-boost regimen may generate an ideal protective effect.

• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1629-1634
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• 2008

A trial was conducted to determine the effect of low crude protein (CP) diets with constant metabolizable energy to crude protein (ME:CP) ratio on growth, body composition and nutrient utilization of broiler chicks from 1 to 26 days of age. Four dietary treatments having four levels of CP and ME as 23, 22, 21 and 20% and 3,036, 2,904, 2,772 and 2,640 kcal/kg, respectively, were formulated and a ME:CP ratio of 132 was maintained in all the diets. Digestible lysine was maintained at 1.10 of the diet. A total of 1,760 day-old Hubbard broiler chicks were randomly divided into 16 experimental units and each diet was offered to four experimental units at random. Feed intake was increased (p<0.05) while weight gain and feed conversion ratio were adversely affected (p<0.05) when the diets with low CP and ME were fed to broilers. Total protein intake and total ME intake were linearly decreased (p<0.05) and protein efficiency ratio and energy efficiency ratio were lower (p<0.05) than in the chicks fed dietary regimen with 22% CP and 2,904 kcal/kg ME. The whole body analysis of the birds revealed that chicks fed the lowest dietary regimens retained less (p<0.05) nitrogen and more ether extract than chicks fed the control diet, however, body dry matter, total body ash and fat free body protein were not affected. Similarly, protein and energy utilization were also unaffected by the dietary treatments. In summary, chicks fed low CP diets with constant ME:CP ratio grew slower, used feed less efficiently and retained less protein and more body fat than chicks fed the control diet.

• Clinical and Experimental Pediatrics
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• 제56권4호
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• pp.159-164
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• 2013

Purpose: Indoxyl sulfate and p-cresyl sulfate are important protein-bound uremic retention solutes whose levels can be partially reduced by renal replacement therapy. These solutes originate from intestinal bacterial protein fermentation and are associated with cardiovascular outcomes and chronic kidney disease progression. The aims of this study were to investigate the levels of indoxyl sulfate and p-cresyl sulfate as well as the effect of probiotics on reducing the levels of uremic toxins in pediatric patients on dialysis. Methods: We enrolled 20 pediatric patients undergoing chronic dialysis; 16 patients completed the study. The patients underwent a 12-week regimen of VSL#3, a high-concentration probiotic preparation, and the serum levels of indoxyl sulfate and p-cresyl sulfate were measured before treatment and at 4, 8, and 12 weeks after the regimen by using fluorescence liquid chromatography. To assess the normal range of indoxyl sulfate and p-cresyl sulfate we enrolled the 16 children with normal glomerular filtration rate who had visited an outpatient clinic for asymptomatic microscopic hematuria that had been detected by a school screening in August 2011. Results: The baseline serum levels of indoxyl sulfate and p-cresyl sulfate in the patients on chronic dialysis were significantly higher than those in the children with microscopic hematuria. The baseline serum levels of p-cresyl sulfate in the peritoneal dialysis group were significantly higher than those in the hemodialysis group. There were no significant changes in the levels of these uremic solutes after 12-week VSL#3 treatment in the patients on chronic dialysis. Conclusion: The levels of the uremic toxins p-cresyl sulfate and indoxyl sulfate are highly elevated in pediatric patients on dialysis, but there was no significant effect by probiotics on the reduction of uremic toxins in pediatric dialysis patients. Therefore, studies for other medical intervention to reduce uremic toxins are also necessary in pediatric patients on dialysis.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.1979-1985
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• 2016

Background: Complementary and alternative medicine has been highly appreciated as a supportive regimen for classical treatment strategies. Here we offer a nutrition-based adjuvant therapy for liver fibrosis, a major risk factor for cirrhosis and hepatocellular carcinoma. Aim of the study: To evaluate the possible hepatoprotective effects of Jerusalem artichoke tubers (JAT) in combination with interferon and ribavirin. Materials and Methods: Twelve groups of rats were administered JAT, interferon and ribavirin either separately or in combination from day one of $CCL_4$ administration until the end of the study. Animals were killed after 8 weeks of $CCL_4$-induced hepatotoxicity. Results: Hepatocytes from rats treated with triple combination of interferon, ribavirin, and JAT showed more less normal architecture compared to $CCL_4$-treated rats. We also detected significantly higher hepatic protein expression levels of p53, BAX and transforming growth factor-${\beta}$ (TGF-${\beta}$) in the $CCL_4$-intoxicated group compared to normal controls, as evidenced by immunohistochemical staining and western blotting analyses. Addition of JAT as a supportive regimen improved response to ribavirin and interferon and effectively participated in retaining normal histopathological and biochemical criteria and significantly lowered protein expression of p53, BAX, and TGF-${\beta}$. Conclusions: We suggest that addition of JAT as a supportive r egimen to interferon and ribavirin effectively potentiates their anti-fibrotic effects.

• Journal of Microbiology and Biotechnology
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• 제25권4호
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• pp.526-536
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• 2015

Leptospirosis is a worldwide zoonotic disease caused by pathogenic Leptospira, a genus of which more than 250 serovars have been identified. Commercial bacterin vaccines are limited in that they lack both cross-protection against heterologous serovars and long-term protection. This study investigated in mice the immunogenicity of an anti-leptospirosis vaccine, using the outer membrane proteins LipL32 and Loa22 as antigens. The immunogenicity of this vaccine formulation was compared with those induced by vaccines based on LipL32 or Loa22 alone. A DNA-encapsulated chitosan nanoparticle was used for in vivo DNA delivery. Using a unique DNA plasmid expressing both lipL32 and loa22 for vaccination, higher antibody responses were induced than when combining plasmids harboring each gene separately. Therefore, this formulation was used to test the immunogenicity when administered by a heterologous prime (DNA)-boost (protein) immunization regimen. The specific antibody responses against LipL32 (total IgG and IgG1) and Loa22 (IgG1) were higher in mice receiving two antigens in combination than in those vaccinated with a single antigen alone. Although no significant difference in splenic CD4⁺ T cell proliferation was observed among all groups of vaccinated mice, splenocytes from mice vaccinated with two antigens exhibited higher interferon-γ and IL-2 production than when using single antigens alone upon in vitro restimulation. Taken together, the immunogenicity induced by LipL32 and Loa22 antigens in a heterologous primeboost immunization regimen using chitosan as a DNA delivery system induces higher immune response, and may be useful for developing a better vaccine for leptospirosis.

• 약학회지
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• 제33권6호
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• pp.345-349
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• 1989

This paper was attempted to investigate effect of angiotensin inhibitor (loading dose 25, 50, $100{\mu}g/kg$ and maintenance dose 12.5, 25, $50{\mu}g/kg/hr$) on the pharmacokinetics of furosemide (5 mg/kg i.v) in rabbit. The plasma concentrations of furosemide increased by angiotensin inhibitor and the relative bioavailability of furosemide increased from 118.1% to 193.2% by the inhibitor. The protein binding of furosemide decreased by angiotensin inhibitor in bovine serum albumin ($2.17\;{\times}\;10^{-4}M$) by equilibrium dialysis method. Consequently, dosage regimen of furosemide might be adjusted carefully when furosemide is administered with angiotensin inhibitor.

• Asian-Australasian Journal of Animal Sciences
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• 제14권5호
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• pp.655-667
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• 2001

This experiment was conducted to investigate the effect of feeding a low CP diet supplemented with synthetic amino acids on performance, nutrient utilization and carcass characteristics of finishing pigs fed under a three-phase feeding regimen. Ninety-six finishing pigs (Landrace$\times$Large White$\times$Duroc), $55.75kg{\pm}0.65$ of initial body weight, were blocked by weight and sex and allotted to four dietary treatments in a randomized block design. There were six pens per treatment and four pigs per pen. Pigs were fed a 16%-14%-12% CP (for phase I-II-III, respectively), sequence of diets. Dietary treatments were 1) Control, 2) Con+L (a sequence of diets reduced in CP by l percentage unit with lysine (L) supplementation, 3) Con+LMT (a sequence of diets reduced in CP by 2 percentage unit with LYS, methionine (MET) and threonine (THE) supplementation) and 4) Con+LMTT (a sequence of diets reduced in CP by 3 percentage unit with LYS, MET, THR and tryptophan (TRP) supplementation). The finishing period (55 to 105 kg) was divided into three phases (55 to 72 kg, 72 to 90 kg and 90 to 105 kg). Pigs fed either the control or Con+L diet grew faster (p<0.05) than pigs fed the Con-LMT or Con+LMTT diet. There was no difference in ADFI among dietary treatments. Phosphorus (P) digestibility was lowest in the control group and highest in the Con+LMTT group (p<0.05). Within each phase, no significant differences in dry matter (DM) and CP digestibilities were found. Although some amino acid digestibilities were affected by dietary treatments, digestibilities of essential amino acids (EAA), non-essential amino acids (NEAA) and total amino acid were not significantly influenced by dietary treatments. For the entire experiment periods, Con+L, Con+LMT and Con+LMTT treatments resulted in 13.4, 18.8 and 21.6% lower total N excretion compared with the control. Con+LMT and Con+LMTT treatments showed significantly lower BUN concentration compared with the control and Con+L treatment (p<0.05), but there was no significant difference in BUN concentration between pigs fed the control and Con+L treatment or between pigs fed Con+LMT and Con+LMTT treatments (p>0.05). Carcass length, backfat thickness and carcass grade were not significantly affected by dietary treatments (p>0.05). In conclusion, reducing dietary CP level by 1 percentage unit and supplementing only LYS at each phase could be a very beneficial feeding strategy for finishing pigs fed under a three phase feeding regimen in terms of both environmental and economical aspects.

• Clinical and Experimental Reproductive Medicine
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• 제47권3호
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• pp.186-193
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• 2020

Objective: The effectiveness of Staphylococcus protein A (SPA) in improving the penetration ability of sperm and reducing antisperm antibody (ASA) titers in immunologically infertile males was evaluated. Methods: Seminal fluid samples were obtained from 15 infertile men, and ASA titers were assessed with the latex agglutination test. Identification of immunoglobulin (Ig) classes and characterization of the antigens involved in the immune response were performed using indirect immunofluorescence. Local ASAs typically present as a mixture of IgG and IgA classes. The capillary tube penetration method was used to assess the capability of spermatozoa to penetrate the cervical mucus (CM). Results: ASAs associated with the neck region of sperm showed a significantly lower migration distance in the CM of infertile females than ASAs associated with the head or tail segments. ASA-positive seminal fluid exhibited significant increases in the mean migration distance (2.6 ± 1.4 cm vs. 1.54 ± 1.1 cm, respectively; p< 0.001) and sperm concentration (174 ± 121.0 × 10³/mL vs. 101 ± 93.7 × 10³/mL, respectively; p= 0.033) after treatment with SPA compared to pre-treated samples. A significant reduction (p< 0.01) in the recorded ASA titer was detected. Conclusion: These results indicate that SPA can be used as a sorting regimen for insemination programs. However, further studies are warranted to assess its influence on pregnancy rate.

• 농업과학연구
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• 제45권2호
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• pp.169-176
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• 2018

This study evaluated the effects of Glycine soja (GS) and hairy vetch supplementation on the quality of corn silage. The feeding regimen consisted of a corn silage (Control) and a control supplemented either with 20% GS or 20% hairy vetch with four replicates. All experimental diets were stored for 40 days at room temperature ($20-25^{\circ}C$). The control had a higher (p < 0.05) dry matter level but had a lower level (p < 0.05) of crude protein, crude fat, acid detergent fiber and neutral detergent fiber than any other treatments. Hairy vetch had a higher level (p < 0.05) of acetic acid and butyric acid than any other groups but had a lower level (p < 0.05) of lactic acid than that of the control. A lower silage pH was observed (p < 0.05) in the control compared to the other treatments. The control had lower (p < 0.05) sucrose and fructose concentrations compared to the other treatments. Corn silage supplemented with hairy vetch showed a higher level (p < 0.05) of crude protein and buffer solution protein and a higher ratio of soluble protein to insoluble protein than any of the other groups. Thus, the results of the present study suggest that GS and hairy vetch supplementation of corn silage would be beneficial for maintaining quality and elevating protein levels when compared to the control.