• 한국식물학회:학술대회논문집
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• 한국식물학회 2001년도 춘계학술발표대회:발표눈문요지록
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• pp.25-25
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• 2001

• 대한한의학회지
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• 제41권2호
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• pp.43-57
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• 2020

Objectives: The purpose of this study was to investigate the effect of Phellinus linteus cheonghyeol plus (PLCP) on antioxidant and inhibition of inflammatory factor expression associated with dyslipidemia in HUVEC. Methods: The scavenging activity of DPPH and ABTS radical of PLCP was measured in HUVEC. The expression levels of NF-κB, p-IκBα, ERK, JNK, and p38 proteins were measured after treating with TNF-α in HUVEC. The expression levels of MCP-1, ICAM-1, and VCAM-1 mRNA and biomarkers were measured after treatment with TNF-α in HUVEC Results: 1. PLCP increases DPPH and ABTS radical scavenging activity in a concentration dependent manner. 2. PLCP significantly decreased the concentration of NF-κB, p-IκBα, ERK, JNK protein compared to the control at concentrations of 100 ㎍/㎖ or more, and significantly decreased concentration of p38 protein at all concentrations. 3. PLCP significantly decreased MCP-1 mRNA expression levels at 100㎍/㎖ or more compared to the control. ICAM-1 and VCAM-1 mRNA expression levels were significantly reduced at all concentrations compared to the control. MCP-1, ICAM-1 protein expression levels were significantly reduced compared to the control at concentrations of 100 ㎍/㎖ or more, and VCAM-1 protein expression levels were reduced at all concentrations. Conclusions: These results suggest that PLCP has an antioxidant effect, and it has been experimentally confirmed that it can prevent or inhibit inflammatory diseases caused by dyslipidemia due to its inhibitory effect on inflammation-related factors in HUVEC.

• Molecules and Cells
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• 제38권7호
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• pp.597-603
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• 2015

Biosynthesis of the phytohormone ethylene is under tight regulation to satisfy the need for appropriate levels of ethylene in plants in response to exogenous and endogenous stimuli. The enzyme 1-aminocyclopropane-1-carboxylic acid synthase (ACS), which catalyzes the rate-limiting step of ethylene biosynthesis, plays a central role to regulate ethylene production through changes in ACS gene expression levels and the activity of the enzyme. Together with molecular genetic studies suggesting the roles of post-translational modification of the ACS, newly emerging evidence strongly suggests that the regulation of ACS protein stability is an alternative mechanism that controls ethylene production, in addition to the transcriptional regulation of ACS genes. In this review, recent new insight into the regulation of ACS protein turnover is highlighted, with a special focus on the roles of phosphorylation, ubiquitination, and novel components that regulate the turnover of ACS proteins. The prospect of cross-talk between ethylene biosynthesis and other signaling pathways to control turnover of the ACS protein is also considered.

• 동아시아식생활학회지
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• 제10권5호
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• pp.394-403
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• 2000

The functional properties of sesame protein concentrate(SPC) using different size of ultrafiltration(UF) membranes were examined and compared with those of conventional acid-precipitated sesame protein concentrate. The protein contents of SPC by UF with molecular size of 10K, 30K and look dalton membranes were 84.2%, 82.7%, and 76.4%, respectively, and that of acid-precipitated SPC was 88.7%. The nitrogen solubility of SPC by UF was higher than that of conventional SPC at various pH levels. Especially, it showed three-fold increase at near isoelectric point. However, water absorption capacity and fat absorption capacity of SPC by UF were decreased. For emulsion and foam properties, there were no significant differences between SPC by acid precipitation and SPC by UF method. At various pH levels, SPC by membrane with pore size of 30K dalton showed the highest emulsion properties. The SPC by UF had slightly higher viscosity than defatted sesame flour and SPC by acid precipitation.

• Asian-Australasian Journal of Animal Sciences
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• 제18권4호
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• pp.502-511
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• 2005

The effects of different levels of crude protein (CP) and cottonseed meal replacing for soybean meal in cassava chips and rice straw-based diets for mid-lactating cows (100-150 day in milk [DIM]), were studied using 32 multiparous Holstein Fresian crossbred dairy cows. Diets containing 10.5, 12.5, 13.7, 14.4% CP of the rations and 0, 12.1, 14.9, 17.8% cottonseed meal were fed to cows for 60 days. Thirty two cows were randomly divided into four dietary treatments using a Randomized complete block design. Four dietary treatments were offered in the form of total mixed ration (TMR) with concentrate to roughage (chopped rice straw) at 60:40 and offered ad libitum. Dry matter (DM) and neutral detergent fiber (NDF) intakes tended to linearly increase with increasing dietary CP levels. Intakes and digestibility of crude protein increased linearly with increasing dietary CP level (p<0.01). Crude protein digestibility of the 10.5% CP diet was lower (p<0.05) than that in diets with higher levels of CP, while there were no significant differences among the other three levels of CP (12.5, 13.7 and 14.4%). Daily milk yield tended to increase with increased CP from 10.5 to 14.4%. Income over feed in terms of US$/kg of milk increased with increased CP from 10.5 to 13.7% and decreased when the CP level was higher than 13.7% (quadratic effect p<0.09). Milk composition was not significantly affected by increasing level of CP, however there were relatively high contents of protein and fat among treatments. The proportion of milk-urea N (MUN), ammonia-N ($NH_3$-N) and bloodurea N (BUN) were closely correlated and increased linearly with increasing CP levels (p<0.01). Balanced diet was found in diet containing 12.5 and 13.7% CP of the rations when BUN and MUN were used as indicators of the protein to energy ratio in the diet. Conclusions can be made that increasing dietary CP levels from 10.5 to 13.7% using cottonseed meal as the main source to completely replace soybean meal was beneficial to cows consuming rice straw and cassava chips based-diets. Increasing the CP level above 13.7% of total ration did not additionally improve milk yield and composition or net income.

• 한국가금학회지
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• 제15권4호
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• pp.261-268
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• 1988

단백질 수준이 각각 18%(초생추 사료)와 12% (비육우 사료)인 사판 사료를 사료 입자 크기로 나누어서 1.1mm이하가 전사료에 대한양외 80%와 40%인 것으로 만든 다음 각각 다른 단백질 수준과 입자 크기가 배합 사료내에 함유된 항곰광이제(0.1% W/W)의 효능에 어떻게 영향을 미치는지에 관하여 연구하였다. 시험 사료내에 함유된 수분의 함량은 12.6% (초생추 사료)와 12.7%(비육우 사료)였다. 시험 사료를 각 처리 별로 1kg씩 나누어 플라스틱통에 넣은 후 뚜껑을 약간 열어둔 상태로 상대습도가 85% 저장 온도를 $29{\pm}$$1^{\circ}C$ 되게 유지시킨 채 5일에서 40일 동안 보전한 후 곰팡이수, 생성된 탄산 가스양 그리고 생성된 아플라 톡신의 양을 측정하였다. 형성된 곰팡이 수(P＜0.05)와 탄산가스 발생양(P＜0.01)은 항곰팡이제가 가해졌을 때 사료의 입자 크기가 1.1mm이하인 것이 전사료의 40%인 구에서 현저히 높았으나 단백질 수준의 차이는 항곰팡이제의 작용에 영향을 미치지 못했다. 그러나 아플라 톡신의 생성은 사료중의 단백질 수준이 높을 수록 많았고(P＜0.05) 또 입자가 큰 것이 많은 사료일수록 현저히 높았다(P＜0.05).

• 한국가금학회지
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• 제14권1호
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• pp.15-24
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• 1987

본 시험은 육계사료의 단백질수준 및 급여시기가 육계의 생산성에 미치는 효과를 구명하기 위하여 전기와 후기사료의 단백질수준을 각각 22%와 20%, 20%와 18%로 하고 전후기사료의 교체시기를 2, 4 및 6주령으로 하는 6개처리에 육계초생추 216수를 공시하여 1984년 12월 14일부터 1985년 2월 8일까지 8주간에 걸쳐 축산시험장에서 시험을 실시한 결과를 요약하면 다음과 같다. 1. 증체량은 단백질수준이 높을수록 증가하였으며, 전후기사료의 교찬시기가 빠를수록 점차 감소하였지만 사료교체시기에서 4주령과 6주령 간에는 차이가 없었다. 2. 사료섭취량은 처리간에 차이가 없었으며, 사료요구율은 전후기 사료의 단백질수준 22% 및 20%를 4주령 또는 6주령에 교체했을 때 가장 유리하였다. 3. 단위증체당 단백질요구량은 단백질수준이 높아질수록 증가하였다. 4. 육성율은 단백질수준이나 교찬시기에 의하여 영향을 받지 않았다. 5. 도체율은 처리간에 차이가 없었지만 복강지방 축적율은 단백질수준이 증가할수록, 그리고 교체시기가 늦어질수록 점차 감소하였다. 6. 수당소득은 전후기사료의 단백질수준 22% 및 20%를 4주령에 교체한 처리에서 가장 높았다. 이상의 결과를 종합해서 볼 때 육계 전기 및 후기사료의 단백질수준은 각각 22%와 20%, 교체시기는 4주령이 적합할 것으로 사료된다.

• Preventive Nutrition and Food Science
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• 제10권3호
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• pp.231-238
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• 2005

A standard $1\%$ w/v solution of CM-chitosan made from squid pen was added to milk at levels of $0.5\sim3\%$ (v/v) to improve the yield and rheological properties of cottage cheese by whey protein retention. Cheese curd did not form at levels higher than $3\%$ (v/v) CM-chitosan standard solution. Yield and total protein of cottage cheese increased up to $2\%\;by\;11\;to\;42\%\;and\;17\;to\;38\%$ respectively, compared to control cheese. Whey protein losses were decreased by 11 to $42\%$ and thus accounted for all of the increase in yield. Anomalous results were obtained at the $0.8\%$ level, which neither improved yield or whey protein retention nor stabilized rheological parameters, and at the $0.5\%$ level, which improved yield and total protein without increasing whey protein retention. Elasticity and cohesiveness of CM-chitosan-containing cheese were generally improved and stabilized during storage. Monitoring of cheese chromaticity values for four weeks revealed a delay in the onset of yellowing in cheeses with CM-chitosan compared to the controls, while the concentration of added CM-chitosan had little influence on cheese chromaticity. The addition of CM-chitosan solution could be applied directly to industrial scale cottage cheese-making without the need for any modification of the production process.

• IMMUNE NETWORK
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• 제11권4호
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• pp.196-202
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• 2011

Background: B cell-activating factor belonging to the TNF family (BAFF) is primarily expressed by macrophages and dendritic cells, and stimulates B cell proliferation, differentiation, survival, and Ig production. In the present study, we explored the effect of activin A on BAFF expression by APCs. Methods: To investigate the effect of activin A on BAFF expression by mouse APCs, we measured the level of BAFF expression at the transcriptional and protein levels using RT-PCR and ELISA. Results: Activin A markedly enhanced BAFF expression in mouse macrophages and dendritic cells at both the transcriptional and protein levels. SB431542, an activin receptor-like kinase 4 (ALK4) inhibitor, completely abrogated activin A-induced BAFF transcription. Furthermore, overexpression of DN-Smad3 abolished activin-induced BAFF expression at the transcriptional and protein levels. Conclusion: These results demonstrate that activin A can enhance BAFF expression through ALK4-Smad3 pathway.

• 한국식품영양과학회지
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• 제22권1호
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• pp.15-18
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• 1993

식이성단백이 methanethiol에 의한 간기능에 어떠한 영향을 미치는지를 검토코자 흰쥐를 저단백식이(LP : casein 7%) 및 고단백식이 (HP : casein 20%)로 1개월간 성장시킨 뒤 methanethiol을 투여한 다음, 간무게, 혈청 ALT 활성을 측정하여 간기능 정도를 두 군간에 비교하였다. 동시에 이에 대한 원인을 규명하는 일환으로 간조직 중 glutahione함량과 glutathione 포함효소의 일종인 glutathione S-transferase (GST)활성을 측정하여 다음과 같은 실험 결과를 얻었다. Methanethiol 투여로 인한 대조군에 대한 간무게와 혈청 ALT활성 증가율은 LP군이 HP군보다 높았으며, 간 glutathione함량과 간 GST활성이 HP군 보다 LP군이 낮게 나타났다. 이상 성적을 종합해 볼 때 methanethiol에 의한 간 독성은 식이 중 단백함량감소에 비례해서 증가되며 이는 LP군이 HP군 보다 methanethiol해독에 관여하는 간 glutathione함량과 GST활성의 감소에 기인되기 때문인 것으로 생각된다.