• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.883-883
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• 2005

• Journal of Life Science
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• 제11권2호
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• pp.92-93
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• 2001

Bacillus sp. KYJ 963, which was isolated from Korean salt-fermented anchovy (anchovy-jeot), produces an extracellular ${\beta}$-amylase. The analysis of the digestion products of substrates by thin layer chromatography from the purified protein revealed that the enzyme could not hydrolyze maltose or ${\alpha}$-cyclodextrin. In the amino acid composition analysis, the major characteristic of the ${\beta}$-amylase was the high proportion of amino acids that possess short side chain such as glycine and alanine.

• Journal of Animal Science and Technology
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• 제61권3호
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• pp.183-183
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• 2019

• Asian-Australasian Journal of Animal Sciences
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• 제12권7호
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• pp.1075-1079
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• 1999

Adult German cross $(German{\times}British{\times}Russian)$ angora rabbits (one year age), 32 in number were divided randomly into four groups $(T_1-T_4)$ with equal sex ratio and fed diets containing $T_1$ groundnut cake (GNC); $T_3$, soyaflakes (SF); $T_4$, sunflower cake (SFC) and $T_2$, a mixture of all the three cakes along with green forage as roughage for a period of 9 months. Nine per cent protein was added from each protein source. Fibre level was maintained by adjusting the level of rice phak in the diets. The diets were iso-nitrogenous and contained similar level of fibre. DMI through roughage was not affected due to source of protein in the diet, however, DMI through concentrate was higher $(p{\leq}0.05)$ with SFC diet, which resulted in higher total feed intake in the group $(T_4)$. Body weights increased up to second shearing, thereafter it decreased due to summer depression. Diet containing soyaflakes sustained higher wool yield whereas, it was lowest $(p{\leq}0.05)$ on SFC diet. Wool attributes (staple length, medullation, fibre diameter) were not affected due to source of protein in the diet. Digestibility of fibre and its fractions (ADF, cellulose, hemicellulose) decreased $(p{\leq}0.05)$ with incorporation of SFC in the diets. Balance of calcium was lowest whereas, that of nitrogen was highest with SFC diet $(T_4)$. Biological value of N and net protein utilization was better when different protein sources were mixed together $(T_2)$. Protein quality of soyaflakes proved better for wool production followed by groundnut cake and mixture of three protein sources. Sunflower cake alone or in combination decreased wool production which may be checked by supplementation of amino acids and energy.

• Asian-Australasian Journal of Animal Sciences
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• 제13권11호
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• pp.1609-1618
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• 2000

Thin stillage and distillers' grains are byproducts remaining after alcohol distillation from a fermented cereal grain mash. Both byproducts are used as energy and protein sources for ruminants. Due to its liquid nature, more than 50% of thin stillage bypasses the rumen. Thin stillage can be fed alone or in combination with distillers' grains. However, a better utilization by beef cattle is anticipated when thin stillage replaces water as a fluid source. Ruminal undegraded protein content of distillers' grains is greatly affected by type of cereal grain and by drying. Corn distillers' grains have a higher ruminal undegraded protein content than wheat distillers' grains while dried distillers' grains have a higher ruminal undegraded protein content than the wet distillers' grains. Wet and dried distillers' grains can replace up to 50% of corn grain in beef cattle diets without affecting animal performance. The estimated NEg of corn distillers' grains for beef cattle ranges from 100 to 169% of that of corn. In general, wet corn distillers' grains have a higher NEg value than dried corn distillers' grains and the addition of thin stillage improves the NEg of distillers' grains. Improved performance of ruminats fed distillers' byproducts can be attributed to high digestible fiber content, improved rumen environment and a shift in organic matter digestion from the rumen to the small intestine.

• Animal cells and systems
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• 제9권2호
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• pp.95-100
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• 2005

Essential hypertension results from the complex interaction between genetic and environmental factors. A C825T polymorphism of the gene encoding G-protein ${\beta}3$ subunit (GNB3), associated with enhanced G-protein coupled signaling and increased $Na^+-H^+$ exchanger, has been implicated in the development of essential hypertension in several human populations, especially in Caucasian population. We examined the disease relevance of this candidate gene by performing an association study in a study group of Korean heritage. Participants comprised 109 essential hypertensives and 109 normotensives, respectively. Genotyping was performed with PCR-BsaJI restriction digestion method. Observed genotype frequencies were in Hardy-Weinberg equilibrium in all groups. Genotype and allele frequencies did not differ significantly between normotensives and essential hypertensives (P>0.05). However, the serum total cholesterol (TC) and LDL-cholesterol levels were significantly higher in subjects with the TT genotype compared to those with the CC or CT genotypes in normotensives of our study subjects (P<0.05). Thus, these results suggest that GNB3/C825T polymorphism might be significantly associated with abnormality in serum lipid metabolism.

• Bulletin of the Korean Chemical Society
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• 제35권10호
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• pp.2985-2989
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• 2014

Autophagy is a self-digestion process in which intracellular structures are degraded in response to stress. Notably, prolonged autophagy leads to cell death. In this study, we investigated whether CX-4945, an orally available protein kinase 2 (CK2) inhibitor, induces autophagic cell death in human cervical cancer-derived HeLa cells and in human prostate cancer-derived LNCaP cells. CX-4945 treatment of both cell lines resulted in the formation of autophagosomes, in the conversion of microtubule-associated protein 1 light chain 3 (LC3), and in down-regulation of the Akt-mammalian target of rapamycin (mTOR)-p70 ribosomal protein S6 kinase (S6K) signaling cascade. Thus, pharmacologic inhibition of CK2 by CX-4945 induced autophagic cell death in human cancer cells by down-regulating Akt-mTOR-S6K. These results suggest that autophagy-inducing agents have potential as anti-cancer drugs.

• Applied Microscopy
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• 제31권2호
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• pp.199-206
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• 2001

완두 자엽세포에 대한 저장단백질 과립의 발달은 단백질 저장 액포의 가장자리에 축적된 단백질의 fragmentation에 의해서 이루어지는 것으로 알려져 왔다. 그러나 최근 이 외에도 terminal dilation, transformation, denovo development등의 독립적인 과정이 각각 다른 시기에 관찰되므로서 단백과립의 발달은 이러한 여러 과정이 모두 나타나는 복합과정임이 알려졌다. 이러한 과정과는 별도로 종자발달의 이른시기에 소포체 내강에 축적되는 저장단백질과 여기서 발달하는 단백과립에 대하여 규명하고자 발달중인 종자의 자엽으로부터 단일 세포를 얻어서 면역세포 화학적 반응을 실시하였다. 그 결과 종자발달의 이른 시기에는 legumin이, 중간시기 이후 에는 vicilin이 축적되므로서 단백질이 축적된 소포체가 단백과립으로 발달하는 것으로 나타났다 소포체 내강에 존재하는 단백질인 $\alpha-Tip$은 비교적 늦은시기에, toneplast membrane protein인 PPase는 이른시기에 각각 면역 세포화학적 반응이 관찰되었다.

• 한국식품영양과학회지
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• 제14권2호
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• pp.202-213
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• 1985

The protein nutritional quality of foods has become an important factor to food processors with the advent of nutritional labeling regulations for foods. Then, as is true today, the officially approved assay for protein nutritional quality was the rat based protein efficiency ratio(PER) bioassay. The PER bioassay requires a minimum of 28 days to performe, and is therefore not applicable to routine quality assurance use by the food industry. Within the past ten years there has been a research emphasis placed on the development of rapid, inexpensive, biological and/or chemical based assays for protein nutritional quality. It was hoped that if a rapid assay could be developed and thoroughly tested, it could be used in lieu of the PER bioassay in the day-to-day quality assurance screening of food ingredients and products. The rapid assays developed in the hope of attaining this goal have been based on microorganisms, proteolytic enzymes, and amino acid profiles, as well as combinations of the above. In this review, it will be described and briefly discussed many of procedures which had contributed conceptually as well as practically to the development of in vitro methods for the evaluation of protein quality. Special emphasis will be placed on the C-PER(computed protein efficiency ratio) assay which combines data from in vitro protease digestion and amino acid composition to predict protein nutritional quality designed by Satterlee et al. (1980), and the DC-PER(discriminant computed PER) which is a method of estimating protein quality based on rat assay and in vitro digestibility obtained using solely essential amino acid data will be also introduced.

• Asian-Australasian Journal of Animal Sciences
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• 제24권1호
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• pp.103-109
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• 2011

The objective of this study was to investigate the effect of enzymolytic products of soybean meal (ESBM), as one of the protein sources in the diet, on the growth performance, nutrient digestibility and immune function of weaned piglets. Soybean meal produced by bioprocessing with fermentation and enzymolysis contains reduced anti-nutritional factors and improved protein utilization. A total of 240 weaned piglets (Duroc${\times}$Landrace${\times}$Yorkshire, $9.01{\pm}0.22\;kg$ body weight) were randomly allocated to 4 treatments with 6 pens per treatment and 10 piglets per pen. The diets were based on corn-soybean meal and ESBM partially replaced soybean meal and soybean protein isolate at the inclusion level of 5, 10 or 15% in the basal diet. Feed intake and body weight were measured weekly. On days 24 to 27, faeces of each replicate were proportionally collected to determine the nutrient digestibility. On day 28 of the experiment, one piglet from each replicate was slaughtered humanely to collect immune organs. The results showed that inclusion of ESBM increased (p<0.05) the final weight, daily feed intake and daily gain of weaned pigs compared with the control diet, and ESBM at the inclusion levels of 10 and 15% improved (p<0.05) the feed/gain compared with the control diet. There were no differences (p>0.05) in daily feed intake among the levels of ESBM, but increasing the levels of ESBM from 5 to 15% improved (p<0.05) the final weight, average daily gain of pigs and feed/gain. The inclusion of ESBM at 5 to 15% increased (p<0.05) the digestibility of crude protein (CP) by 5 to 16%, and ESBM at 15% increased (p<0.05) the digestibility of digestible energy (DE), Ca and P compared with the control diet. ESBM increased (p<0.05) the relative weights of thymus and mandibular lymph nodes by 57.7 and 29.6%, respectively. The percentages of T lymphocytes, CD4+ and CD8+ in peripheral blood of weaned piglets were also increased (p<0.05) by feeding ESBM. The results suggest that ESBM can be a better protein source in improving growth performance, nutrient digestibility and immune function of weaned piglets.