• 대한임상검사과학회지
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• 제46권2호
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• pp.75-78
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• 2014

Green tea and tea polyphenols have been studied extensively as cancer chemopreventive agents in recent years. Epigallocatechin-3-gallate (EGCG) is widely recognized as a powerful antioxidant and a free radical scavenger. The purpose of this study was to evaluate the protective effects of green tea catechins (GTC) on the Bleomycin- and Cyclophosphamide-induced cytotoxicity. Cell viability was measured by MTT assay. In the protective effect of GTC, the cell viability was significantly increased by the treatment of GTC. Furthermore, GTC showed the higher protective effect than EGCG and vitamin E. These results suggest that GTC has the protective effect which is related to the prevention of cancer. Our studies show that the continuous presence of EGCG can reduce radical-induced DNA damage in Chinese hamster lung fibroblast cells (CHL cells).

• International Journal of Advanced Culture Technology
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• 제12권2호
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• pp.258-269
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• 2024

This study aims to examine the effects of child care teachers' job stress and personality on child abuse protective behaviors, which consist of perception and reporting intention of child abuse, and to verify the moderating effect of past child abuse experience. A survey was conducted on 246 child care teachers nationwide, and the collected data were analyzed through regression analysis and t-test. The results showed that job stress had a negative effect and personality had a positive effect on child abuse protective behaviors. In addition, there was a significant difference in the influence of job stress and personality on protective behaviors according to the presence or absence of past child abuse experience. Based on these results, practical implications for preventing child abuse by child care teachers were discussed.

• 대한약침학회지
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• 제10권1호통권22호
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• pp.121-135
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• 2007

Objectives : This study was carried out to examine protective effect of wild ginseng extract on HepG2 human hepatoma cell line against tert-Butyl hydroperoxide (t-BHP)-induced oxidative damage. Methods : To evaluate protective effect of wild ginseng extract against t-BHP induced cytotoxicity, LDH level and activity of glutathione peroxidase and reductase were measured. Gene expression was also measured using DNA microarray. Results : Wild ginseng extract showed a significant protective effect against t-BHP-induced cytotoxicity in HepG2 cell line. It is not, however, related with the activities of glutathione peroxidase and glutathione reductase. Analysis of gene expression using DNA chip, demonstrated that 28 genes were up-regulated in t-BHP only group. Five genes - selenoprotein P, glutathione peroxidase 3, sirtuin 2, peroxiredoxin 2, serfiredoxin 1 homolog - may be related with the protective effect of wild ginseng extract. Conclusions : Based on the results, a protective effect of wild ginseng extract against t-BHP-induced oxidative damage in HepG2 cell line is not associated with the activities of glutathione peroxidase and glutathione reductase, but with the expression of selenoprotein P, glutathione peroxidase 3, sirtuin 2, peroxiredoxin 2, and serfiredoxin 1 homolog.

• Biomolecules & Therapeutics
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• 제4권4호
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• pp.428-436
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• 1996

The protective effect of Carthamus tinctorius L. Semen on the carbon tetrachloride induced liver damaged rats were studied. First, methanol extract was prepared and the extract was fractionated with hexane, $CHCl_3$, BuOH and $H_2O$ respectively. Animals were divided into 6 groups and each group was treated with each fraction respectively. To investigate the hepato-protective effect of Carthamus tinctorius L. Semen AST, ALT, albumin, TP, cholesterol, TG, creatinine and total bilirubin values were measured in each treated group and compared with those of control group. GST activity was increased in BuOH group compared with the control group. In malondialdehyde levels, all fractions was decreased compared with the control group. In histopathologic examination, hexane and $H_2O$ fractions of Carthamus tinctorius L. Semen observed mild degree of ballooning degeneration. The results show the protective effect of Hexane,$CHCl_3$, BuOH and $H_2O$ fractions on hepatotoxicity of $CHl_4$ by decreasing ALT, AST, bilirubin, cholesterol, TG and BUN. It seems that the decrease of MDA are related to the recovery effect. The protective effects of Carthamus tinctorius L. Semen fractions in hepatotoxic pathogenesis by $CHl_4$ was suggested in blood chemistry analysis and histopathologic examination.

• Natural Product Sciences
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• 제12권3호
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• pp.134-137
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• 2006

Four lignan compounds including gomisin J (1), schizandrin (2), gomisin A (3), and angeloyl gomisin H (4) have been isolated from the MeOH extract of Schizandra chinensis fruits. The evaluation for protective effect of compounds 1-4 against tert-butyl hydroperoxide (t-BHP)-induced cytotoxicity in hippocampal HT22 cell line was conducted. Compound 1 showed significant protective effect with an $EC_{50}$ value of $43.3{\pm}2.3\;{\mu}M$, whereas compounds 2-4 were inactive. Trolox, one of the well-known antioxidant, used as a positive control, and also showed protective effect with an $EC_{50}$ value of $213.8{\pm}8.4\;{\mu}M$. These results suggest that compound 1 may possess the neuroprotective activity against oxidant-induced cellular injuries.

• 동의생리병리학회지
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• 제24권6호
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• pp.1042-1052
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• 2010

This study aimed to evaluate the protective effects of Mundongcheongpye-eum (MCE) on elastase-induced lung injury. The extract of MCE was treated to A549 cells and elastase-induced lung injury mice model. Then, various parameters such as cell-based cyto-protective activity and histopathological finding were analyzed. MCE showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, protein level of cyclin B1, Cdc2, and Erk1/2, and gene expression of TNF-${\alpha}$ and IL-$1{\beta}$ in A549 cells. MCE treatment also revealed the protective effect on elastase-induced lung injury in mice model. This effect was evidenced via histopathological finding including immunofluence stains against elastin, collagen, caspase 3, and protein level of cyclin B1, Cdc2, and Erk1/2 in lung tissue. These data suggest that MCE has a pharmaceutical properties on lung injury. This study would provide an scientific evidence for the efficacy of MCE for clinical application to patients with chronic obstructive pulmonary disease.

• 한국치위생학회지
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• 제22권4호
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• pp.259-272
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• 2022

Objectives: To identify the factors influencing the turnover intention of registered South Korean dental hygienists and analyze the effect sizes. Methods: Overall, 54 studies based on criteria of PICO from 1 January 2000 to 11 January 2022 were extracted. The factors related to turnover intention were applied to the ecological system theory and categorized. Subsequently, the effect size of the correlations was comprehensively meta-analyzed by dividing it into protective and risk factors depending on the negative or positive correlation direction. Results: For the overall effect size, the protective factor (ES_r=-0.458) was higher than the risk factor (ES_r=0.352). In the protective factors, organizational commitment and perceived organizational support belonging to the microsystem yielded the largest effect size. Furthermore, as for risk factors, burnout, job stress, work harassment, role conflict, and emotional labor belonging to the microsystem showed a moderate effect size. Conclusions: Factors belonging to the microsystem demonstrated a large effect size in both protection and risk factors for dental hygienists' turnover intention. Additionally, the factor showing the largest effect size was protective factor categorized into a microsystem.

• 디지털융복합연구
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• 제15권12호
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• pp.59-70
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• 2017

본 연구는 중도입국 청소년의 학교적응에 영향을 미치는데 있어 위험요인에 대한 보호요인의 조절효과가 있는지를 알아보고자 하였다. 이를 위해 2015년 전국다문화가족실태조사 대상 중 만 9~24세 청소년 자녀 6,079명 데이터에 가중치를 적용한 69,720명을 분석하였다. 분석결과 첫째, 중도입국 청소년의 위험요인인 차별경험, 학교폭력, 우울은 학교적응에 모두 부적인 영향을 미쳤다. 보호요인인 부모관계, 자아존중감, 한국어 능력, 국적취득은 학교적응에 모두 정적인 영향을 보였다. 둘째, 중도입국 청소년의 학교적응에 있어 3개의 위험요인 가운데 우울을 조절하는 보호요인 중에는 자아존중감과 국적취득 변인, 학교폭력을 조절하는 보호요인은 부모관계, 자아존중감, 한국어 능력, 국적취득 변인으로 분석되었다. 본 연구의 분석결과를 토대로 중도입국 청소년에 대한 우울감소, 학력폭력 대처, 다양한 역량강화 프로그램 개발과 지원을 주요 사회복지적 실천방안으로 제시하였다.

• 공업화학
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• 제29권1호
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• pp.49-55
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• 2018

본 연구에서는 그라비올라의 주성분인 nicotiflorin을 분리하고 그 아글리콘 성분인 kaempferol을 얻어 세포 보호 효과 및 그 보호 메커니즘을 규명하였다. L-Ascorbic acid 및 (+)-${\alpha}$-tocopherol을 대조군으로 하여, $^1O_2$로 유도된 세포 손상에 대해 nicotiflorin 및 kaempferol의 보호 효과를 측정한 결과 nicotiflorin < (+)-${\alpha}$-tocopherol < kaempferol 순으로 보호 효과가 증가하였다. L-Ascorbic acid는 세포 보호 효과를 보이지 않았다. 이들의 세포 보호 효과 메커니즘을 밝히기 위해 singlet oxygen 소광 속도 상수, 자유라디칼 소거 활성, ROS 소거 활성 및 적혈구 세포 침투율을 측정하였다. 실험 결과, kaempferol과 그 배당체인 nicotiflorin의 세포 보호 효과에 있어서 큰 차이는 세포막에의 침투가 가장 큰 요인으로 확인되었다. 대조군 L-ascorbic acid가 항산화능은 크지만 실험 조건에서 세포막에 침투가 잘 안되어 세포 보호 효과가 나타나지 않은 것으로 확인되었다. Kaempferol과 (+)-${\alpha}$-tocopherol의 비교를 통해 세포 침투뿐만 아니라 라디칼 소거활성 및 ROS 소거 활성도 세포 보호 효과에 기여하는 것으로 나타났다. 결론적으로, 광증감 반응으로 유도된 세포막 파괴에 대한 보호작용은 항산화제들의 세포 침투, 자유라디칼 및 ROS 소거 활성이 큰 영향을 미치는 것으로 확인되었다.

• 한국위험물학회지
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• 제6권2호
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• pp.55-61
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• 2018

In this study, the molten metal protective performance of various molten metal protective clothing materials such as herringbone twill laminated aluminium foils, oxydized-polyacrylonitrile laminated with aluminium deposited polyethylene terephthalate films, twill fabric laminated with aluminium deposited polyethylene terephthalate films and nonwoven laminated with aluminum deposited polyethylene terephthalate films, were evaluated according to modified EN ISO 9185. The results showed that the molten metal protective performance of tested samples improved with the increase in fabric structure density, weight and thickness. In addition the effect of the thickness of aluminum foil on the molten metal protective performance is not significant. It was found the fabric is more important in the molten metal splash protective clothing.