• 한국환경과학회지
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• 제30권2호
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• pp.185-189
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• 2021

This study was conducted to determine and to standardize the time of emergence of Protaetia brevitarsis adults, through four breeding experiments, including one which selected the dominant individuals of Protaetia brevitarsis. The breeding experiments were divided into two groups with hibernating and non-hibernating Protaetia brevitarsis by placing 100, 150, 200, 250 and 300 Protaetia brevitarsis in each breeding box (50 cm × 35 cm × 15 cm) filled with 12 L of fermented sawdust. Breeding tests were with four replicates each for 3 months. The test period was classified into four stages: Exp. 1 (Sep 1, 2019 to Nov 30, 2019), Exp. 2 (Dec 1, 2019 to Feb 29, 2020), Exp. 3 (Mar 1, 2019 to May 31, 2020) and Exp. 4 (Jun 1, 2020 to Aug 31, 2020). The survey items included average dead number, average emergence number, average mortality, and average emergence rate. In Exp. 1 and 2, the changes in Protaetia brevitarsis adults were similar. In Tables 1 and 2, the allegorical changes of adult white-spotted flowers were similar. In addition, the average dead number, average emergence number, average mortality, and average emergence rate showed better results in the hibernation state than in both Exp. 1 and 2. Exp. 3 and 4 showed a pattern of change different from the results of Exp. 1 and 2 and these characteristics were superior in the hibernation states compared to the non-hibernation states. In conclusion, this result was found to be most appropriate as a standardization of the emergence time of 200 Protaetia brevitarsis treatments through four breeding experiments in the hibernating state.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권3호
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• pp.180-187
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• 2021

This study was performed to assess Protaetia brevitarsis larva excrement as an organic fertilizer for corn cultivation. Furthermore, the study investigated insect communities in each treatment. In 2009, the corn growth rate was worst in soil treated with P. brevitarsis larva excrement and in 2010, the corn growth rate was worst in untreated soil. From the outcome of the study, P. brevitarsis larva excrement could be utilized as organic fertilizer for corn cultivation.

• 한국환경과학회지
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• 제28권7호
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• pp.639-643
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• 2019

We aimed to evaluate antithrombotic efficacy of Protaetia brevitarsis extract during 21 days. Rats (SPF rat, weight 240~260 g) were divided into 16 groups (5 rats per group), they were: control group and Protaetia brevitarsis extract groups with dose of 0.1, 0.5, 1, 2.5, 5, 10, 25, 50, 75, 100, 200, 250, 500, 750, 1,000 mg/kg kg of body weight. Thromboplastin time (PT) and activated partial thromboplastin time (aPPT) as antithrombotic efficacy were tested in this animal experiment (at 7, 14 and 21 days). Overall, the admistration dose of Protaetia brevitarsis extract over 50 mg/kg at 7, 14 and 21 days for PT and over 25 mg/kg at 7, 14 and 21 days for aPPT tented to be longer than that of other groups. In addition, the optimal admistration doses of Protaetia brevitarsis extract to improves antithrombotic efficacy were 75, 100, 200 and 250 mg/kg at 7, 14 and 21 days for PT (p<0.05) and 50 and 100 mg/kg at 7 days, 75 mg/kg at 14 days, or 50, 100, 200 and 250 mg/kg at 21 days for aPPT (p<0.05). It can be concluded that Protaetia brevitarsis extract at optimal levels have antithrombotic efficacy.

• 대한의생명과학회지
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• 제26권4호
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• pp.267-274
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• 2020

The larva of Protaetia brevitarsis Lewis (P. brevitarsis), edible insect, is traditionally consumed as alternative source of nutrients and has various health benefits. However, the exact pharmaceutical effects of P. brevitarsis on inflammatory response are still not well understood. Thus, we investigated the anti-inflammatory effects and mechanisms of P. brevitarsis in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. We investigated the effects of P. brevitarsis on the expression levels of inflammatory-related genes, including inflammatory cytokines, prostaglandin E2 (PGE2), cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) in LPS-stimulated RAW264.7 cells. To understand the anti-inflammatory mechanism of P. brevitarsis, we explored the regulatory effect of P. brevitarsis on nuclear factor (NF)-κB and caspase-1 activation. The findings of this study demonstrated that P. brevitarsis inhibits the LPS-induced inflammatory cytokine and PGE2 levels, as well as COX-2 and iNOS expression. Moreover, we confirmed that the anti-inflammatory effect of P. brevitarsis occurs via suppression of the activation of NF-κB and caspase-1. Conclusively, these findings provide experimental evidence that P. brevitarsis may be useful candidate for the treatment of inflammatory-related diseases.

• 한국응용곤충학회지
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• 제44권4호
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• pp.283-286
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• 2005

우리나라에서 한약재로 활용되고 있는 흰점박이꽃무지(Protaetia brevitarsis)와 장수풍뎅이(Allomyrina dichotoma)의 산란과 발육에 관여하는 환경요인을 밝히고자 본 시험을 실시하였다. 부엽토수분함량은 두 종 모두에서 과습이나 과습은 산란을 저하시켰고 산란 시 최적의 수분함량은 $65{\sim}75%$로 추정되었다. 부엽토 수분함량은 흰점박이꽃무지와 장수풍뎅이의 알 및 유충의 생존율과 발육기간, 그리고 유충의 섭식활동에 크게 영향을 주는 것으로 이들의 생육에 관여하는 가장 중요한 요인이었다.

• 생명과학회지
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• 제23권5호
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• pp.664-668
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• 2013

본 연구에서는 흰점박이꽃무지 유충의 식 약용으로써의 가능성을 제시하기 위해 영양성분분석 및 유해물질 검사를 실시하였다. 일반성분은 건량 기준 조단백질 함량이 57.86%로 매우 높았으며, 조지방 16.57%, 조섬유 8.36%, 조회분 5.38%로 구성됨을 확인하였다. 또한 불포화 지방산 중 혈압강하작용 및 여러 약리기전이 알려져 있는 oleic acid (omega-6)가 전체지방산 중 60% 이상을 차지하고 있었고, linoleic acid (omega-6) 5.22%, arachidonic acid (omega-6) 0.25% 함유되어 있었다. 유해물질의 분석 결과, 중금속의 일종인 수은이 0.07 mg/kg으로 존재하였으나 일반식품(어패류의 경우, 0.5 mg/kg) 허용 기준치 이하의 미량인 것으로 확인되었으며, 병원성 미생물인 대장균 O157:H7과 살모넬라균은 검출되지 않았으므로 식용으로 사용하기에 문제가 없는 것으로 판단되었다. 따라서 흰점박이꽃무지 유충은 고함량의 단백질과 필수아미노산 및 생체 내에서 합성할 수 없는 영양소들을 다량 함유하고 있으므로 육류나 어류를 대신할 수 있는 대체식품으로 활용 가능할 것으로 판단되며 안전한 식품소재로의 기준 및 규격마련을 통해 식 약용으로 이용 가능할 것이라 기대된다.

• 대한방사선기술학회지:방사선기술과학
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• 제44권2호
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• pp.117-122
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• 2021

This study is desinged to examine for radiation protection effect of Protaetia Brevitarsis Larvae extracts on the blood and prostate of male rat as a natural radiation protection agent. 5 groups were classified using 90 male rat as experimental animals. Each group was clssified as normal control group (NC Group), the group administered protaetia brevitarsis larvae extracts (PBE Group), irradiated group (IR Group), irradiated group after administration of protaetia brevitarsis larvae extracts (PBE+IR Group), the group administered protaetia brevitarsis larvae extracts after irradiaton (IR+PBE Group). In IR Group, 7 Gy/h of Co-60 gamma ray was irradiated to SD rats. In PBE+IR Group, protaetia brevitarsis larvae extacts wewe injected at 200 mg/kg/day for 14 days before irradiation, In IR+PBE Group, protaetia brevitarsis larvae extract was injeted after irradiation. On the 1, 7 and 21 days after irradiation, the experimental animals were sacrificed to evaluate the changes in blood cell component, superoxide dismutase (SOD) activity, histopathological evaluation of the liver and prostate gland. As a result, the PBE+IR Group and IR+PBE Group showed a significantly recovery of white blood cell (p<0.01, p<0.01), platelet (p<0.01, p<0.01) than the IR Group. It was also confirmed that SOD activity of PBE+IR Group (p<0.01) and IR+PBE Group (p<0.01) was significantly increased than the IR Group. Also PBE+IR Group and IR+PBE Group showed less inflammatory reactions of cystoplasm in the prostate gland than the IR Group. In conclusion, the protaetia brevitarsis larvae have radioprotection effect against blood and prostate gland. It is expected to be useful for research of radiation protection agent.

• 동의생리병리학회지
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• 제19권5호
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• pp.1337-1343
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• 2005

The hepato-protective effects of the extract from Protaetia brevitarsis against hepatotoxicity by carbon tetrachloride ($CCI_4$) were studied in rats. The rats were orally treated with $CCI_4$ (50% in corn oil) at initial dose of $1\;m{\ell}/kg$ followed by $0.5m{\ell}/kg$ four times during 2-week period. The extract of P. brevitarsis (50, 100 or 200 mg/kg) or its vehicle was administered day after day from 1 week before $CCI_4$ Injection during five weeks. $CCI_4$ induced hepato-celluar degeneration and necrosis induced to increase in serum aspartate amintransferase (AST) and alanine aminotransferase (ALT) levels. In biochemical analyses, thiobarbituric acid-reactive substances (TBARS) and antioxidant enzymes such as superoxide dismutase (SOD) and catalase in hepatic tissues were remarkably increased by $CCI_4$ treatment. Not only increases in serum AST and ALT, but also induction of lipid peroxidation and antioxidant enzymes in hepatic tissues caused by $CCI_4$ were significantly attenuated by the P. brevitarsis extract in a dose-dependent manner. Such hepato-protective effects of P. brevitarsis extract were confirmed by histopathological examinations, wherein only mild hepatocytic vacuolations were observed in the liver of rats treated with a high dose (100 mg/kg) of P. brevitarsis extract in comparison with severe hepatocytic degenerations administered with $CCI_4$ alone. From these results, it is suggested that the extract of Protaetia brevitarsis could be a promising candidate for the protection of liver injury, based on the preventive effects against morphological cellular injuries, lipid peroxidation and serum biochemical parameters.

• 한국환경과학회지
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• 제30권2호
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• pp.191-194
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• 2021

This study aimed to provide information and data for the management of insect breeding farms by identifying the appropriate density when rearing Protaetia brevitarsis larvae. The breeding box of the insects was filled with 2 L of fermented sawdust on a 50 × 35 × 15 cm sheet of plastic, and the density of the treatments was 200, 300 and 400 P. brevitarsis in the first, second, and third larval stages. Each treatment was repeated five times, and the sawdust medium was replaced three times (10 intervals). The experiments were conducted for a total of 30 days from March 1, 2020. Overall, 200 P.brevitarsis in the first, second, and third larval stages reared in the breeding box had a higher average survival rate and lower average mortality rate, thereby contributing to efficient production. In conclusion, this result suggests a way to increase production efficiency through the environmental management of insect breeding farms.

• 한국환경과학회지
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• 제29권7호
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• pp.785-788
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• 2020

We investigated the oviposition characteristics of Protaetia brevitarsis seulensis, focusing on zone breeding throughout the year in terms of the development and utilization of insect resources. To select Protaetia brevitarsis seulensis individuals laying a large number of eggs, the number of eggs laid for each individual was measured for each individual after emergence as adults from larvae grown at room temperature in five farmhouses. The five study zone included Gongju (Tancheon), Gyeonggi (Himsen), Chungnam (Gyeryong), Taean (Wonbuk), and Siheung (Baekse). The average oviposition tendency during a 12-week period obtained from the five regions was somewhat different; however, there was no remarkable difference in overall tendency. The maximum oviposition in Protaetia brevitarsisseulensis in the five regions occurred between 6 and 7 weeks. Compared toother regions, the average cumulative number of eggs laid during the 12-week period was higher (40%-60%) in Gongju (Tancheon) and Gyeonggi (Himsen). These results indicate that in two regions, zone breeding is selected as a system for increasing the demand for insects.